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The map label for this gene is whiG [H]

Identifier: 78221650

GI number: 78221650

Start: 476380

End: 477132

Strand: Direct

Name: whiG [H]

Synonym: Gmet_0429

Alternate gene names: 78221650

Gene position: 476380-477132 (Clockwise)

Preceding gene: 78221649

Following gene: 78221651

Centisome position: 11.92

GC content: 61.09

Gene sequence:

>753_bases
ATGAACTGTCTGGTAAAGGCATACGAGCAGGAGGCTCAGCGTTCGTCGGCCCAGTCCCGCGACGAGCTGATCGTTACCCA
TCTCCCCCTGGTGAAATTTCTGGTGGGACGGATCGCGACCCAGTTACCCCCCCACCTGGACCAGGATGATCTCATGAGCG
CCGCCGTTATCGGTCTCATCACCGCCGCCGAGCGGTTCGACCCATCCCGGGGGGTGCAGTTCAAGACCTTTGCCGAACAG
CGGATCAGGGGGACCATCATCGACGAGCTCCGTTCCCAGGACTGGCTCACCCGGTCCCTGCGGGACAAGTTCAAGAAGCT
GGAGCGGGAGTTCGTGATGCTGGAGCACCGGCTCGGCCGCAACCCAACCAGCGAAGAGGTGGCCAAGTCCCTGGAAATCA
GCGTGGACGACTACCACCACATGCTGGAGGAGATTCACCTCCTCTCCTTCGTTAGTCTCGACGAGTCGTGGGAGGACGAG
GACGGCAGCCCCTTCGGGCTTCTGGATATCCTGGAGGATAAGGGGGTCGAGAATCCCCAGAGCCAGCTCATGGCCCGGCA
GATGCTCGATACGCTCACCGAGGCCATCGAGGGACTCCCCGAGAAAGAGCGGCTCGTGATCACCTTCTACTACTACGAGG
AACTGAACCTGAAGGAGATCGGCGCGGTGCTTGATCTCTCCGAGTCGCGCATCTCCCAGCTCCACAGCCAGGCCATCGTC
CGTCTGCGGGCGAAGATGAAGAACATGAGGTGA

Upstream 100 bases:

>100_bases
CCCGGCGGATCCTGGAAAACGGCGGCGAGCACCGGCTCAAGGGCAACGTGCAGTTCTTCTTCAGGAGATTCCTCAGCGCC
GACGCCGAAATGGAGAACGT

Downstream 100 bases:

>100_bases
ATCCATGAACAGCGGTATCTATTCGGCACTCACGGGGAACGTCGCGGCCATGAAACGGCTCGATGTGCTGTCCAACAACC
TGGCCAACGTCAACACCCCT

Product: sigma 28 (flagella/sporulation)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNCLVKAYEQEAQRSSAQSRDELIVTHLPLVKFLVGRIATQLPPHLDQDDLMSAAVIGLITAAERFDPSRGVQFKTFAEQ
RIRGTIIDELRSQDWLTRSLRDKFKKLEREFVMLEHRLGRNPTSEEVAKSLEISVDDYHHMLEEIHLLSFVSLDESWEDE
DGSPFGLLDILEDKGVENPQSQLMARQMLDTLTEAIEGLPEKERLVITFYYYEELNLKEIGAVLDLSESRISQLHSQAIV
RLRAKMKNMR

Sequences:

>Translated_250_residues
MNCLVKAYEQEAQRSSAQSRDELIVTHLPLVKFLVGRIATQLPPHLDQDDLMSAAVIGLITAAERFDPSRGVQFKTFAEQ
RIRGTIIDELRSQDWLTRSLRDKFKKLEREFVMLEHRLGRNPTSEEVAKSLEISVDDYHHMLEEIHLLSFVSLDESWEDE
DGSPFGLLDILEDKGVENPQSQLMARQMLDTLTEAIEGLPEKERLVITFYYYEELNLKEIGAVLDLSESRISQLHSQAIV
RLRAKMKNMR
>Mature_250_residues
MNCLVKAYEQEAQRSSAQSRDELIVTHLPLVKFLVGRIATQLPPHLDQDDLMSAAVIGLITAAERFDPSRGVQFKTFAEQ
RIRGTIIDELRSQDWLTRSLRDKFKKLEREFVMLEHRLGRNPTSEEVAKSLEISVDDYHHMLEEIHLLSFVSLDESWEDE
DGSPFGLLDILEDKGVENPQSQLMARQMLDTLTEAIEGLPEKERLVITFYYYEELNLKEIGAVLDLSESRISQLHSQAIV
RLRAKMKNMR

Specific function: The level of this sigma factor may be crucial in determining the developmental fate of hyphae [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=223, Percent_Identity=47.085201793722, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789098, Length=255, Percent_Identity=28.6274509803922, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789448, Length=204, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28840; Mature: 28840

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCLVKAYEQEAQRSSAQSRDELIVTHLPLVKFLVGRIATQLPPHLDQDDLMSAAVIGLI
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TAAERFDPSRGVQFKTFAEQRIRGTIIDELRSQDWLTRSLRDKFKKLEREFVMLEHRLGR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPTSEEVAKSLEISVDDYHHMLEEIHLLSFVSLDESWEDEDGSPFGLLDILEDKGVENPQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHH
SQLMARQMLDTLTEAIEGLPEKERLVITFYYYEELNLKEIGAVLDLSESRISQLHSQAIV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHH
RLRAKMKNMR
HHHHHHHCCC
>Mature Secondary Structure
MNCLVKAYEQEAQRSSAQSRDELIVTHLPLVKFLVGRIATQLPPHLDQDDLMSAAVIGLI
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TAAERFDPSRGVQFKTFAEQRIRGTIIDELRSQDWLTRSLRDKFKKLEREFVMLEHRLGR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPTSEEVAKSLEISVDDYHHMLEEIHLLSFVSLDESWEDEDGSPFGLLDILEDKGVENPQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHH
SQLMARQMLDTLTEAIEGLPEKERLVITFYYYEELNLKEIGAVLDLSESRISQLHSQAIV
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHH
RLRAKMKNMR
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2507166; 12000953 [H]