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The map label for this gene is ddl [H]

Identifier: 78221636

GI number: 78221636

Start: 459049

End: 460932

Strand: Direct

Name: ddl [H]

Synonym: Gmet_0415

Alternate gene names: 78221636

Gene position: 459049-460932 (Clockwise)

Preceding gene: 78221635

Following gene: 78221637

Centisome position: 11.48

GC content: 61.57

Gene sequence:

>1884_bases
ATGACCCGTGATGAACTGAAAACCAAGAAGATCGGCGTGCTTATGGGGGGGCTCTCGGCGGAACGGGAGATCTCTCTCTC
CAGCGGAGGAGCGGTGCACAAGGCGCTTCTGGCCCGGGGATATGACGCCGTTGCCATTGACGTGGACCGCGATCTGCCCC
AGGTCCTTGTCAGAGAGTGGATCGACGTGGCCTTCATCTGCCTCCACGGCCGCTACGGCGAAGACGGGGCGGTCCAGGGG
GTGCTGGAACTCATGGGAATCCCCTACACCGGCTCGGGGGTCCTTGCGAGCGCCCTGGCCATGAACAAGATTTTCGCCAA
GCAGGCCTTTGCCGCCGCAGGCCTCACGATAACCCCCCACCGGGTGCTCGTGCGGGGCGAAACCTTCGACGCCGTTGCGG
CCGGCATTGATCTTCCCGTAGTGGTGAAGCCCTCCCAGGAGGGGTCGTCGGTGGGGGTAAGCATCGTGAAGAAGGCGGAG
GAGCTTGCCGCCGCTCTGGAGGCCGCGTTCCGCTACGACAACGAAATCCTCGTGGAGAAGTTCGTCAAGGGGCGGGAGAT
TCAAGTAGGGATTCTCGACGACCGGGCCATGGGAGCCATCGAGATTGTGCCGAAGAAGGAGTTCTACGACTTCGAGGCCA
AATACACCGACGGCATGGCGGAGCACATCTGTCCGCCGGTTCTTCCCGCCGACCTGTACCGCAAGCTCCTCGCCGAAGGG
GAGAAGGCCCATCGGGCTTTGGGGTGCGCCGGGTACAGCCGGGTAGACTTCCTGGTGACCGAGGGGGGTGACTGTTTTCT
CCTCGAAGTGAATACCCTGCCGGGGATGACGGCCCTGAGCCTCCTGCCGGAGATCGCCCAGAAGGAGGCGGGGATCGCTT
TCGAGGAGCTGGTGGAGCGGATACTGGTTTCAGCTTCGCTGAAGATTCAGGGACCGGGGACCGGGGACCGGGTTCAAAAG
AACACTAGGAATCACAGGGGGAACGCTGGGCGTGGAGCTGGTTGTTTGTTTTTGCCGGTCCCTGGTCCCTGGTCCCCGAT
CACCGATTTTATGCGCGATTTACACGTAAAAACACGAATCCAGCCGGCCAACAAGAGCCGCAACCGGGTAAAGAAAGAGC
GGAAGCCGATCAACTGGCGTCCCCTCATCACCTGGGGGAGCCGGGCTGTGCTCGGCGCGTTGGCCGTGGCGGTCGTGGGG
GGCGCAGGCTACAAGGGGTACGCGTTCGCCTCCCGGTACGAGATTGCGGTACTGCAGGTGGAGGCCATCGAGGTGTCGAA
GTTGAGGCACCTGACTCGTGACGAAGTTCTTGGACAGGCCGGGGTGAGAAGGGGCGATTCCATGCTGGGGCTGCGGTTAC
GCCGCATCGGCGAACAACTGGCCAAGAACCCCTGGATCGAGAAGGTGCAGGTGCGCCGCTACTTCCCCCACACCATCCGC
ATCGAGGTGGTGGAGCGGGAACCGGTGGCTGTGGTGAACATGGGGTTTCTCTATTACCTGGATGCCAAGGGGGAGGTCTT
TAAGCCCCTTACCCAGGGTGACAGCCTCAATTTTCCGGTCATAACCGGCATCACCGAGGATGATCTGGCCCGGGATCCCA
AGGGGGCCAGGGAGATGCTCACCGGCGCCGTGGCCCTCATGGATATGCTGAAGAAGGGGCGCGCCTTTACCCTGGCCGAT
GTTTCGGAGGTTCACATCGACAAGGGATTCGGCCTCACTCTCTTCACCGCCGGTGGAGGCGTGCCGGTACGGCTCGGCAA
GGATGGGTACGAGACCAAACTGGCCCGTTTTGCCACGGTCTACGGGGAACTAAAGACGCAGATGACGGCAGTTGAGTACA
TCGATTGCGACTACCAGGACAAGATCATCGTCAAAAAAGGATAA

Upstream 100 bases:

>100_bases
GTAAAACAACGTACCGGCATAACTCTTGAAGAAGAGGTCAGGATTGTCGGTGAAGAGTGATTAAAGAACCGCTTCCAAGA
AGCGCTCAGGCGAAAACGCG

Downstream 100 bases:

>100_bases
AGCGGGGGAGGGGGAAACACTATGTCGGCACGAAGGGATAACCTGATCGTCGGGCTCGATATCGGCACAACCAAGATTTG
TGCCATCGTCGGCAACGTCA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MTRDELKTKKIGVLMGGLSAEREISLSSGGAVHKALLARGYDAVAIDVDRDLPQVLVREWIDVAFICLHGRYGEDGAVQG
VLELMGIPYTGSGVLASALAMNKIFAKQAFAAAGLTITPHRVLVRGETFDAVAAGIDLPVVVKPSQEGSSVGVSIVKKAE
ELAAALEAAFRYDNEILVEKFVKGREIQVGILDDRAMGAIEIVPKKEFYDFEAKYTDGMAEHICPPVLPADLYRKLLAEG
EKAHRALGCAGYSRVDFLVTEGGDCFLLEVNTLPGMTALSLLPEIAQKEAGIAFEELVERILVSASLKIQGPGTGDRVQK
NTRNHRGNAGRGAGCLFLPVPGPWSPITDFMRDLHVKTRIQPANKSRNRVKKERKPINWRPLITWGSRAVLGALAVAVVG
GAGYKGYAFASRYEIAVLQVEAIEVSKLRHLTRDEVLGQAGVRRGDSMLGLRLRRIGEQLAKNPWIEKVQVRRYFPHTIR
IEVVEREPVAVVNMGFLYYLDAKGEVFKPLTQGDSLNFPVITGITEDDLARDPKGAREMLTGAVALMDMLKKGRAFTLAD
VSEVHIDKGFGLTLFTAGGGVPVRLGKDGYETKLARFATVYGELKTQMTAVEYIDCDYQDKIIVKKG

Sequences:

>Translated_627_residues
MTRDELKTKKIGVLMGGLSAEREISLSSGGAVHKALLARGYDAVAIDVDRDLPQVLVREWIDVAFICLHGRYGEDGAVQG
VLELMGIPYTGSGVLASALAMNKIFAKQAFAAAGLTITPHRVLVRGETFDAVAAGIDLPVVVKPSQEGSSVGVSIVKKAE
ELAAALEAAFRYDNEILVEKFVKGREIQVGILDDRAMGAIEIVPKKEFYDFEAKYTDGMAEHICPPVLPADLYRKLLAEG
EKAHRALGCAGYSRVDFLVTEGGDCFLLEVNTLPGMTALSLLPEIAQKEAGIAFEELVERILVSASLKIQGPGTGDRVQK
NTRNHRGNAGRGAGCLFLPVPGPWSPITDFMRDLHVKTRIQPANKSRNRVKKERKPINWRPLITWGSRAVLGALAVAVVG
GAGYKGYAFASRYEIAVLQVEAIEVSKLRHLTRDEVLGQAGVRRGDSMLGLRLRRIGEQLAKNPWIEKVQVRRYFPHTIR
IEVVEREPVAVVNMGFLYYLDAKGEVFKPLTQGDSLNFPVITGITEDDLARDPKGAREMLTGAVALMDMLKKGRAFTLAD
VSEVHIDKGFGLTLFTAGGGVPVRLGKDGYETKLARFATVYGELKTQMTAVEYIDCDYQDKIIVKKG
>Mature_626_residues
TRDELKTKKIGVLMGGLSAEREISLSSGGAVHKALLARGYDAVAIDVDRDLPQVLVREWIDVAFICLHGRYGEDGAVQGV
LELMGIPYTGSGVLASALAMNKIFAKQAFAAAGLTITPHRVLVRGETFDAVAAGIDLPVVVKPSQEGSSVGVSIVKKAEE
LAAALEAAFRYDNEILVEKFVKGREIQVGILDDRAMGAIEIVPKKEFYDFEAKYTDGMAEHICPPVLPADLYRKLLAEGE
KAHRALGCAGYSRVDFLVTEGGDCFLLEVNTLPGMTALSLLPEIAQKEAGIAFEELVERILVSASLKIQGPGTGDRVQKN
TRNHRGNAGRGAGCLFLPVPGPWSPITDFMRDLHVKTRIQPANKSRNRVKKERKPINWRPLITWGSRAVLGALAVAVVGG
AGYKGYAFASRYEIAVLQVEAIEVSKLRHLTRDEVLGQAGVRRGDSMLGLRLRRIGEQLAKNPWIEKVQVRRYFPHTIRI
EVVEREPVAVVNMGFLYYLDAKGEVFKPLTQGDSLNFPVITGITEDDLARDPKGAREMLTGAVALMDMLKKGRAFTLADV
SEVHIDKGFGLTLFTAGGGVPVRLGKDGYETKLARFATVYGELKTQMTAVEYIDCDYQDKIIVKKG

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=46.2046204620462, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1786579, Length=344, Percent_Identity=32.2674418604651, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 68403; Mature: 68272

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDELKTKKIGVLMGGLSAEREISLSSGGAVHKALLARGYDAVAIDVDRDLPQVLVREW
CCHHHHHHHHHEEEECCCCCCCEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
IDVAFICLHGRYGEDGAVQGVLELMGIPYTGSGVLASALAMNKIFAKQAFAAAGLTITPH
HHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
RVLVRGETFDAVAAGIDLPVVVKPSQEGSSVGVSIVKKAEELAAALEAAFRYDNEILVEK
EEEEECCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FVKGREIQVGILDDRAMGAIEIVPKKEFYDFEAKYTDGMAEHICPPVLPADLYRKLLAEG
HHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHCCCCHHHCCCCCCCHHHHHHHHHCC
EKAHRALGCAGYSRVDFLVTEGGDCFLLEVNTLPGMTALSLLPEIAQKEAGIAFEELVER
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
ILVSASLKIQGPGTGDRVQKNTRNHRGNAGRGAGCLFLPVPGPWSPITDFMRDLHVKTRI
HHHHCEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEEE
QPANKSRNRVKKERKPINWRPLITWGSRAVLGALAVAVVGGAGYKGYAFASRYEIAVLQV
CCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEEE
EAIEVSKLRHLTRDEVLGQAGVRRGDSMLGLRLRRIGEQLAKNPWIEKVQVRRYFPHTIR
EHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEE
IEVVEREPVAVVNMGFLYYLDAKGEVFKPLTQGDSLNFPVITGITEDDLARDPKGAREML
EEEEECCCEEEEEECEEEEECCCCCEECCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHH
TGAVALMDMLKKGRAFTLADVSEVHIDKGFGLTLFTAGGGVPVRLGKDGYETKLARFATV
HHHHHHHHHHHCCCEEEEECHHHHEECCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
YGELKTQMTAVEYIDCDYQDKIIVKKG
HHHHHHHHEEEEEECCCCCCCEEEECC
>Mature Secondary Structure 
TRDELKTKKIGVLMGGLSAEREISLSSGGAVHKALLARGYDAVAIDVDRDLPQVLVREW
CHHHHHHHHHEEEECCCCCCCEEECCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
IDVAFICLHGRYGEDGAVQGVLELMGIPYTGSGVLASALAMNKIFAKQAFAAAGLTITPH
HHEEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCC
RVLVRGETFDAVAAGIDLPVVVKPSQEGSSVGVSIVKKAEELAAALEAAFRYDNEILVEK
EEEEECCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FVKGREIQVGILDDRAMGAIEIVPKKEFYDFEAKYTDGMAEHICPPVLPADLYRKLLAEG
HHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHCCCCHHHCCCCCCCHHHHHHHHHCC
EKAHRALGCAGYSRVDFLVTEGGDCFLLEVNTLPGMTALSLLPEIAQKEAGIAFEELVER
HHHHHHHCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
ILVSASLKIQGPGTGDRVQKNTRNHRGNAGRGAGCLFLPVPGPWSPITDFMRDLHVKTRI
HHHHCEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHEEE
QPANKSRNRVKKERKPINWRPLITWGSRAVLGALAVAVVGGAGYKGYAFASRYEIAVLQV
CCCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEEE
EAIEVSKLRHLTRDEVLGQAGVRRGDSMLGLRLRRIGEQLAKNPWIEKVQVRRYFPHTIR
EHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEE
IEVVEREPVAVVNMGFLYYLDAKGEVFKPLTQGDSLNFPVITGITEDDLARDPKGAREML
EEEEECCCEEEEEECEEEEECCCCCEECCCCCCCCCCCCEEECCCHHHHCCCCCHHHHHH
TGAVALMDMLKKGRAFTLADVSEVHIDKGFGLTLFTAGGGVPVRLGKDGYETKLARFATV
HHHHHHHHHHHCCCEEEEECHHHHEECCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
YGELKTQMTAVEYIDCDYQDKIIVKKG
HHHHHHHHEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA