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The map label for this gene is murD

Identifier: 78221631

GI number: 78221631

Start: 453140

End: 454498

Strand: Direct

Name: murD

Synonym: Gmet_0410

Alternate gene names: 78221631

Gene position: 453140-454498 (Clockwise)

Preceding gene: 78221630

Following gene: 78221632

Centisome position: 11.34

GC content: 64.53

Gene sequence:

>1359_bases
ATGGAAATTTCGGGAAAGAACATACTGGTCGTAGGCCTTGCCCGCACGGGCGTGGCGGTGGCCCGCTTTCTGGCGCGAAA
CGGGGCGCGGGTGACCGTGACCGACCTTCGCGACGAGTCCGCCCTGGCCGGTCCCCTCCGGGAGCTGGCAGGACTTTCCG
TGCGGTATGTGCTAGACCGCCACGATGAGGCTGATTTTGCTGCTGCCGACGTAGTGGTGGTGAGTCCCGGCGTCCCCCAG
GAGAGCCCCTATCTCCAGGCGGCCCGCAGGGCCGGACGTGAGGTGATTACCGAGATCGAACTGGCGTCCCGGTTCGTTAC
TGTCCCCATGGTGGCCATCACCGGGACCAACGGCAAGACCACCACCACGACCCTGACAGGGGAGATCTTCAGCGCCTGCG
GGTTCCGGACCTTCGTGGGGGGGAACATCGGCAATCCTCTCATCGAGCTTGTAGAGGGAGGCGAGACGGTGGAACGGGTG
GTGGTGGAGATCAGTTCCTTCCAGCTGGAATGGATCAGCTCCTTCCGCCCGCGGGTGGCGGTGCTCCTCAATATCACCGA
GGACCACCTGGACCGCTATGCTACGTTCCAGGAGTACATTGACGCCAAGGTGCGGATCTTCGAGAACCAGGAATCGACTG
ACTACGCCGTGCTGAACGTGGATGATCCCATCGTGGCCGGCATTGCCGGGCGGGTTGCCGCGACGGTATTCCCCATGAGC
CAACAGCGGGAGCTTGCCGAGGGGGTCTTCCACCGGGACGGCGTCATCACCTTCCGGCACCAGGGGCGTGAGGAACGCTT
CCCCACCGCCCGATTCCGGATCACCGGAGTCCACAATATCGAAAACATCATGGCCTCCCTCGCCGCGACCCTCCTCCTGG
GGTGCGACGCAAAACAGGCCCTTGCGGCCGTTGAAGGGTTCGGCGGGCTCCCCCACCGGATGGAGCTGGTGCGGGAGTTG
GCAGGCGTCCGTTACTACGAGGACAGCAAAGCCACCAACGTGGGAAGTGTGGAGAAGGCCCTGGCCAGTTTCAATGACAT
CACCCTCATTGCCGGAGGGAAGGACAAGGGGGGATCGTATGCACCCCTGGCCCCCCTGGTGACGGAGCGGGTGCGGCACA
TGGTCCTCATCGGCGAGGCAAAGGAACGGATGGCCCGGGAACTGGGGAGCCTTACCGACACCCGCATGGCCACGACCCTC
GAGGAAGCGGTCGAGCTGGCAGCGAGCCTCACGGAGCCGGGAGGGGTAGTCCTCTTCTCGCCAGCCTGCTCCAGTTTCGA
CATGTTCCGTGACTACGAGGAACGGGCCCAGCGTTTCCGCGCGGCCGTAAACGCCCTCGGCACGGGAGAGCGCCCGTGA

Upstream 100 bases:

>100_bases
GAGCTTAAGGGTGTGGCGGAGCCGAAGATCATCGTGCGGTTCTGGATCATCACCATTATCCTGGCCCTGGTGGCGATCTC
GACCTTGAAGCTGAGATAGC

Downstream 100 bases:

>100_bases
ACCTGAACTTCAAACTGAACCTGAAAGAGATCGAGCGTTACGACCTGGTCATACTCCTGATGGCGGTGGCCCTTACCTGC
TTCGGGGTGGTGATGGTCTA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEISGKNILVVGLARTGVAVARFLARNGARVTVTDLRDESALAGPLRELAGLSVRYVLDRHDEADFAAADVVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFVTVPMVAITGTNGKTTTTTLTGEIFSACGFRTFVGGNIGNPLIELVEGGETVERV
VVEISSFQLEWISSFRPRVAVLLNITEDHLDRYATFQEYIDAKVRIFENQESTDYAVLNVDDPIVAGIAGRVAATVFPMS
QQRELAEGVFHRDGVITFRHQGREERFPTARFRITGVHNIENIMASLAATLLLGCDAKQALAAVEGFGGLPHRMELVREL
AGVRYYEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAPLVTERVRHMVLIGEAKERMARELGSLTDTRMATTL
EEAVELAASLTEPGGVVLFSPACSSFDMFRDYEERAQRFRAAVNALGTGERP

Sequences:

>Translated_452_residues
MEISGKNILVVGLARTGVAVARFLARNGARVTVTDLRDESALAGPLRELAGLSVRYVLDRHDEADFAAADVVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFVTVPMVAITGTNGKTTTTTLTGEIFSACGFRTFVGGNIGNPLIELVEGGETVERV
VVEISSFQLEWISSFRPRVAVLLNITEDHLDRYATFQEYIDAKVRIFENQESTDYAVLNVDDPIVAGIAGRVAATVFPMS
QQRELAEGVFHRDGVITFRHQGREERFPTARFRITGVHNIENIMASLAATLLLGCDAKQALAAVEGFGGLPHRMELVREL
AGVRYYEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAPLVTERVRHMVLIGEAKERMARELGSLTDTRMATTL
EEAVELAASLTEPGGVVLFSPACSSFDMFRDYEERAQRFRAAVNALGTGERP
>Mature_452_residues
MEISGKNILVVGLARTGVAVARFLARNGARVTVTDLRDESALAGPLRELAGLSVRYVLDRHDEADFAAADVVVVSPGVPQ
ESPYLQAARRAGREVITEIELASRFVTVPMVAITGTNGKTTTTTLTGEIFSACGFRTFVGGNIGNPLIELVEGGETVERV
VVEISSFQLEWISSFRPRVAVLLNITEDHLDRYATFQEYIDAKVRIFENQESTDYAVLNVDDPIVAGIAGRVAATVFPMS
QQRELAEGVFHRDGVITFRHQGREERFPTARFRITGVHNIENIMASLAATLLLGCDAKQALAAVEGFGGLPHRMELVREL
AGVRYYEDSKATNVGSVEKALASFNDITLIAGGKDKGGSYAPLAPLVTERVRHMVLIGEAKERMARELGSLTDTRMATTL
EEAVELAASLTEPGGVVLFSPACSSFDMFRDYEERAQRFRAAVNALGTGERP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=36.8763557483731, Blast_Score=245, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_GEOMG (Q39YM1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383378.1
- ProteinModelPortal:   Q39YM1
- STRING:   Q39YM1
- GeneID:   3738165
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0410
- NMPDR:   fig|269799.3.peg.438
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q39YM1
- ProtClustDB:   CLSK829090
- BioCyc:   GMET269799:GMET_0410-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49050; Mature: 49050

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISGKNILVVGLARTGVAVARFLARNGARVTVTDLRDESALAGPLRELAGLSVRYVLDR
CCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECC
HDEADFAAADVVVVSPGVPQESPYLQAARRAGREVITEIELASRFVTVPMVAITGTNGKT
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCE
TTTTLTGEIFSACGFRTFVGGNIGNPLIELVEGGETVERVVVEISSFQLEWISSFRPRVA
EEEEECHHHHHHCCCHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VLLNITEDHLDRYATFQEYIDAKVRIFENQESTDYAVLNVDDPIVAGIAGRVAATVFPMS
EEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCH
QQRELAEGVFHRDGVITFRHQGREERFPTARFRITGVHNIENIMASLAATLLLGCDAKQA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCHHHH
LAAVEGFGGLPHRMELVRELAGVRYYEDSKATNVGSVEKALASFNDITLIAGGKDKGGSY
HHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCC
APLAPLVTERVRHMVLIGEAKERMARELGSLTDTRMATTLEEAVELAASLTEPGGVVLFS
CCHHHHHHHHHHHEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PACSSFDMFRDYEERAQRFRAAVNALGTGERP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEISGKNILVVGLARTGVAVARFLARNGARVTVTDLRDESALAGPLRELAGLSVRYVLDR
CCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEECC
HDEADFAAADVVVVSPGVPQESPYLQAARRAGREVITEIELASRFVTVPMVAITGTNGKT
CCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCE
TTTTLTGEIFSACGFRTFVGGNIGNPLIELVEGGETVERVVVEISSFQLEWISSFRPRVA
EEEEECHHHHHHCCCHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VLLNITEDHLDRYATFQEYIDAKVRIFENQESTDYAVLNVDDPIVAGIAGRVAATVFPMS
EEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCH
QQRELAEGVFHRDGVITFRHQGREERFPTARFRITGVHNIENIMASLAATLLLGCDAKQA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCHHHH
LAAVEGFGGLPHRMELVRELAGVRYYEDSKATNVGSVEKALASFNDITLIAGGKDKGGSY
HHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCC
APLAPLVTERVRHMVLIGEAKERMARELGSLTDTRMATTLEEAVELAASLTEPGGVVLFS
CCHHHHHHHHHHHEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
PACSSFDMFRDYEERAQRFRAAVNALGTGERP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA