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The map label for this gene is mraW

Identifier: 78221625

GI number: 78221625

Start: 445791

End: 446732

Strand: Direct

Name: mraW

Synonym: Gmet_0404

Alternate gene names: 78221625

Gene position: 445791-446732 (Clockwise)

Preceding gene: 78221624

Following gene: 78221626

Centisome position: 11.15

GC content: 62.53

Gene sequence:

>942_bases
GTGGCCGAGTTTGGCCATCTGTCGGTCATGCCGGCGGAAGTGCTTGCCCTGCTCTCCCCCCGGCCGGGCGGAGTTTATGT
GGATGGGACCCTGGGAGGCGCCGGTCATGCCCGGATGATTCTTGAGGCGTCTTCGCCCGATGGCGTTCTGGTCGGGTTCG
ACCGGGACCCGGAGGCGTTGGCCGCGGCCAGCAAGCGGATCGCCTCCTTCGGGGACCGGGTAAGGCTCGTTCGATCAAAT
TTTGCAGCCATGGACGAGTCGCTTGCCACGCTGGGGATTGACACCATCGACGGCTTCCTCCTGGATCTTGGTGTTTCATC
CCACCAGCTTGATACGGCGGAGCGGGGGTTCAGTTTTCAGGTGGATGCACCCCTTGATATGAGGATGGACCCGGAAACCG
GCGAGACTGCTGCCGATCTGGTGAATGAACTTTCTGCAGACGAGCTGGCTCGAATAATCCGAGAGTACGGAGAAGAACGG
TGGGCGAAGCGGATCGCCTCGTTCGTCGTCAAGGCGAGGGAGCAGCACGGGCCCATAGAGAGTACGCTTCAGCTGGTTGA
TTGTGTGAAGGGGGCGATTCCCCGGGCAGCGTGGGAAGAGCGAATCCATCCGGCCACGCGGACGTTCCAGGCGCTCCGCA
TTGCTGTGAACGACGAACTCGGAAGCCTGGAGCGGGGCCTTGTGGCCGGTATCGAACGTCTGGCCCCGGGGGGGAGGGGG
GTGGTGATTTCGTTCCACTCCCTTGAGGATCGGATCGTCAAGACCGTTTTCCGCCGCTATGCCCAGGGATGCACCTGCCC
GAAAATGCTCCCCCGATGTGTATGCGGCAACAAACCTCGCGTCCGCATCCTTACCGGAAAGCCGGTAATGGCGGGAGGGG
ACGAGGTGGCGACAAACCCTAGGGCGCGGAGCGCCAAGGTGCGGGCAGTGGAAAAGCTGTGA

Upstream 100 bases:

>100_bases
AAGTGGAAATCTGGAGCCAGTCCGAATGGGATAAGGTGCGTGCCCATGATATGAAGGCGTTCCCCTCGGACTCCGAGGCG
GTGGCGGAGCTGGGTCTCTA

Downstream 100 bases:

>100_bases
GAGTTATTGGAGGGAGTATGGCGCAGGCGAGAACCGATTTTACCAAGGTGGCGGCACCGAAAAAGCTTGAGGCGATGTAT
GCCCAGAGGGGGGAGATGTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAEFGHLSVMPAEVLALLSPRPGGVYVDGTLGGAGHARMILEASSPDGVLVGFDRDPEALAAASKRIASFGDRVRLVRSN
FAAMDESLATLGIDTIDGFLLDLGVSSHQLDTAERGFSFQVDAPLDMRMDPETGETAADLVNELSADELARIIREYGEER
WAKRIASFVVKAREQHGPIESTLQLVDCVKGAIPRAAWEERIHPATRTFQALRIAVNDELGSLERGLVAGIERLAPGGRG
VVISFHSLEDRIVKTVFRRYAQGCTCPKMLPRCVCGNKPRVRILTGKPVMAGGDEVATNPRARSAKVRAVEKL

Sequences:

>Translated_313_residues
MAEFGHLSVMPAEVLALLSPRPGGVYVDGTLGGAGHARMILEASSPDGVLVGFDRDPEALAAASKRIASFGDRVRLVRSN
FAAMDESLATLGIDTIDGFLLDLGVSSHQLDTAERGFSFQVDAPLDMRMDPETGETAADLVNELSADELARIIREYGEER
WAKRIASFVVKAREQHGPIESTLQLVDCVKGAIPRAAWEERIHPATRTFQALRIAVNDELGSLERGLVAGIERLAPGGRG
VVISFHSLEDRIVKTVFRRYAQGCTCPKMLPRCVCGNKPRVRILTGKPVMAGGDEVATNPRARSAKVRAVEKL
>Mature_312_residues
AEFGHLSVMPAEVLALLSPRPGGVYVDGTLGGAGHARMILEASSPDGVLVGFDRDPEALAAASKRIASFGDRVRLVRSNF
AAMDESLATLGIDTIDGFLLDLGVSSHQLDTAERGFSFQVDAPLDMRMDPETGETAADLVNELSADELARIIREYGEERW
AKRIASFVVKAREQHGPIESTLQLVDCVKGAIPRAAWEERIHPATRTFQALRIAVNDELGSLERGLVAGIERLAPGGRGV
VISFHSLEDRIVKTVFRRYAQGCTCPKMLPRCVCGNKPRVRILTGKPVMAGGDEVATNPRARSAKVRAVEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=42.5655976676385, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI165377202, Length=210, Percent_Identity=43.3333333333333, Blast_Score=145, Evalue=4e-35,
Organism=Escherichia coli, GI1786270, Length=320, Percent_Identity=44.0625, Blast_Score=239, Evalue=1e-64,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=37.5375375375375, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=37.5375375375375, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_GEOMG (Q39YM7)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383372.1
- ProteinModelPortal:   Q39YM7
- STRING:   Q39YM7
- GeneID:   3739384
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0404
- NMPDR:   fig|269799.3.peg.432
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q39YM7
- ProtClustDB:   PRK00050
- BioCyc:   GMET269799:GMET_0404-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33839; Mature: 33708

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFGHLSVMPAEVLALLSPRPGGVYVDGTLGGAGHARMILEASSPDGVLVGFDRDPEAL
CCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEECCCCHHHH
AAASKRIASFGDRVRLVRSNFAAMDESLATLGIDTIDGFLLDLGVSSHQLDTAERGFSFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHCCCEEE
VDAPLDMRMDPETGETAADLVNELSADELARIIREYGEERWAKRIASFVVKAREQHGPIE
ECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
STLQLVDCVKGAIPRAAWEERIHPATRTFQALRIAVNDELGSLERGLVAGIERLAPGGRG
HHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCE
VVISFHSLEDRIVKTVFRRYAQGCTCPKMLPRCVCGNKPRVRILTGKPVMAGGDEVATNP
EEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCC
RARSAKVRAVEKL
CCCCHHHHHHHCC
>Mature Secondary Structure 
AEFGHLSVMPAEVLALLSPRPGGVYVDGTLGGAGHARMILEASSPDGVLVGFDRDPEAL
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEEECCCCHHHH
AAASKRIASFGDRVRLVRSNFAAMDESLATLGIDTIDGFLLDLGVSSHQLDTAERGFSFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCHHHCCCEEE
VDAPLDMRMDPETGETAADLVNELSADELARIIREYGEERWAKRIASFVVKAREQHGPIE
ECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
STLQLVDCVKGAIPRAAWEERIHPATRTFQALRIAVNDELGSLERGLVAGIERLAPGGRG
HHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCCCE
VVISFHSLEDRIVKTVFRRYAQGCTCPKMLPRCVCGNKPRVRILTGKPVMAGGDEVATNP
EEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHCCCC
RARSAKVRAVEKL
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA