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The map label for this gene is narH [H]

Identifier: 78221551

GI number: 78221551

Start: 368998

End: 370452

Strand: Direct

Name: narH [H]

Synonym: Gmet_0330

Alternate gene names: 78221551

Gene position: 368998-370452 (Clockwise)

Preceding gene: 78221550

Following gene: 78221552

Centisome position: 9.23

GC content: 64.67

Gene sequence:

>1455_bases
ATGGATGTCAGAGCACAACTGGTAATGGTGTTCAACCTGGACAAATGCATCGGCTGCCACGCCTGCAGCCTCTCCTGCAA
GAACATCTGGACCGACCGCAAGGGCGCCGAATACATGTGGTGGAACAACGTGGAGACGAAACCCGGCGTCGGCTACCCGA
AGAAGTGGGAAGACCAGGAGGAGTTCAAGGGGGGATGGGTCGCCGACGGGCATAACCTGCGGCTCAAGGCCATGGGGAAG
GGGCCGACGCTCCTCAACATGTTCTTCCAGCCCAACATGCCGAAGCTGGAGGACTACTACGAGCCCTTCGACTTCGACTA
CGGCAACCTCTACGGGGCCAAGGGGGGGGATGACCAGCCCGTGGCCGAGGCCTTTTCCCAGATCACCGGGGAGAAGATGG
CCCATATCAAGGCCGGCCCCAACTGGGACGACGACCTCTCCGGCTCCCAGGTCTACGCGTCGGCAGACGTGAACCTGGAG
GACAAAAAGATCATCGAGCAGTACAGCACGATGTTCATGCAGTACCTCCCCCGCATCTGCAACCACTGCGCGAACCCGGC
CTGCGTCGCCTCCTGCCCGTCCCGGGCCATCTACAAGCGGGGGGAGGACGGGGTGGTTCTGGTGGACCAGGAGGTCTGCA
AGGGGTGGCGGTTCTGCACGAGCGCCTGCCCCTACAAGAAGGTCTACTTCAACTGGAACAGCGGCAAGGCGGAGAAGTGC
ATCTTCTGCTTCCCCCGGACCGAGACCGGCCAGTGCAACGCCTGCGCCCACAGCTGCGTCGGCCGTATCCGCTACGTGGG
GGTCCTCCTCTACGACGCCGAGAAGGTGGAGCAGGCGCTCCTGAAGCCCGACAACCAGCTCGTCGAGGCCCATCGGGGGG
TGATCCTCGATCCCCGGGACGAGGCGGTCCGGGAAGGGGCGAGGAAGAATGGCGTCCCCGAGCAGTGGCTGGAGGCCGCC
GAGAAGTCGCCGGTCTATGCCTTGATCAAGGAGTTCGAACTCGCCCTGCCGCTCCACCCCGAGTTTCGGACCATGCCCAT
GGCCTACTACATCCCCTCCCTCTCGCCGGTTCTGACCTCCGGCGGCGAGCTCCACGAGCTGGTGGAGCACGGCACGATCC
CGACCCGGGAGGCCCTGCGCGCCCCCATCGGGTACCTGGCGAGCCTCTTCGGGGGCGGCAACCGGGACGTGGTGGCGGCG
GCCCTGGAGAAGCTCATCGCCCTTCGCATCCACATGCGGGCCAAGAACCTCAACCAGCCCACGGACGAGGCGAGCCTCGG
GAAGGCCGGGCTCGACGAGGCAGGGGCGAAACGTCTCTACCGTCTCTTCACCATCGGCGGCTACAACGAGCGGAACATCA
TTCCGCCCCAGCAGCGGGAAGAGCTCGATTCCCACACCCGCAAGGGGGCCAGCGGCTTCGGCATTCTCAAGAAGACGGGG
AGGGGAAAACGATGA

Upstream 100 bases:

>100_bases
GGGGGCTACGCCCAGTTCAGCTACTACTTCAACTACTGGGGACCGACCGGCGTGAACCGGGACAGCTACGTGGTCGTCAG
AAAGCTGAGGGGGTGAGACA

Downstream 100 bases:

>100_bases
CAGCGGATATCTATTCATCCCTCGCCCGGATGCTCGACTATCCCCAGGAGAAAAAGAGCCTCCTGTGGGCCATCGGCACC
GTCCAGAGGTATCTGAGCGA

Product: respiratory nitrate reductase beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDVRAQLVMVFNLDKCIGCHACSLSCKNIWTDRKGAEYMWWNNVETKPGVGYPKKWEDQEEFKGGWVADGHNLRLKAMGK
GPTLLNMFFQPNMPKLEDYYEPFDFDYGNLYGAKGGDDQPVAEAFSQITGEKMAHIKAGPNWDDDLSGSQVYASADVNLE
DKKIIEQYSTMFMQYLPRICNHCANPACVASCPSRAIYKRGEDGVVLVDQEVCKGWRFCTSACPYKKVYFNWNSGKAEKC
IFCFPRTETGQCNACAHSCVGRIRYVGVLLYDAEKVEQALLKPDNQLVEAHRGVILDPRDEAVREGARKNGVPEQWLEAA
EKSPVYALIKEFELALPLHPEFRTMPMAYYIPSLSPVLTSGGELHELVEHGTIPTREALRAPIGYLASLFGGGNRDVVAA
ALEKLIALRIHMRAKNLNQPTDEASLGKAGLDEAGAKRLYRLFTIGGYNERNIIPPQQREELDSHTRKGASGFGILKKTG
RGKR

Sequences:

>Translated_484_residues
MDVRAQLVMVFNLDKCIGCHACSLSCKNIWTDRKGAEYMWWNNVETKPGVGYPKKWEDQEEFKGGWVADGHNLRLKAMGK
GPTLLNMFFQPNMPKLEDYYEPFDFDYGNLYGAKGGDDQPVAEAFSQITGEKMAHIKAGPNWDDDLSGSQVYASADVNLE
DKKIIEQYSTMFMQYLPRICNHCANPACVASCPSRAIYKRGEDGVVLVDQEVCKGWRFCTSACPYKKVYFNWNSGKAEKC
IFCFPRTETGQCNACAHSCVGRIRYVGVLLYDAEKVEQALLKPDNQLVEAHRGVILDPRDEAVREGARKNGVPEQWLEAA
EKSPVYALIKEFELALPLHPEFRTMPMAYYIPSLSPVLTSGGELHELVEHGTIPTREALRAPIGYLASLFGGGNRDVVAA
ALEKLIALRIHMRAKNLNQPTDEASLGKAGLDEAGAKRLYRLFTIGGYNERNIIPPQQREELDSHTRKGASGFGILKKTG
RGKR
>Mature_484_residues
MDVRAQLVMVFNLDKCIGCHACSLSCKNIWTDRKGAEYMWWNNVETKPGVGYPKKWEDQEEFKGGWVADGHNLRLKAMGK
GPTLLNMFFQPNMPKLEDYYEPFDFDYGNLYGAKGGDDQPVAEAFSQITGEKMAHIKAGPNWDDDLSGSQVYASADVNLE
DKKIIEQYSTMFMQYLPRICNHCANPACVASCPSRAIYKRGEDGVVLVDQEVCKGWRFCTSACPYKKVYFNWNSGKAEKC
IFCFPRTETGQCNACAHSCVGRIRYVGVLLYDAEKVEQALLKPDNQLVEAHRGVILDPRDEAVREGARKNGVPEQWLEAA
EKSPVYALIKEFELALPLHPEFRTMPMAYYIPSLSPVLTSGGELHELVEHGTIPTREALRAPIGYLASLFGGGNRDVVAA
ALEKLIALRIHMRAKNLNQPTDEASLGKAGLDEAGAKRLYRLFTIGGYNERNIIPPQQREELDSHTRKGASGFGILKKTG
RGKR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=479, Percent_Identity=49.8956158663883, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1787740, Length=478, Percent_Identity=47.6987447698745, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI1787872, Length=108, Percent_Identity=43.5185185185185, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787122, Length=108, Percent_Identity=43.5185185185185, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789370, Length=119, Percent_Identity=31.9327731092437, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 54136; Mature: 54136

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRAQLVMVFNLDKCIGCHACSLSCKNIWTDRKGAEYMWWNNVETKPGVGYPKKWEDQE
CCCCEEEEEEEECHHHHCCHHHCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
EFKGGWVADGHNLRLKAMGKGPTLLNMFFQPNMPKLEDYYEPFDFDYGNLYGAKGGDDQP
HHCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCCCCEECCCCCCCCH
VAEAFSQITGEKMAHIKAGPNWDDDLSGSQVYASADVNLEDKKIIEQYSTMFMQYLPRIC
HHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NHCANPACVASCPSRAIYKRGEDGVVLVDQEVCKGWRFCTSACPYKKVYFNWNSGKAEKC
HHCCCCCEECCCCCHHHHHCCCCCEEEECHHHHCHHHHHHCCCCCEEEEEEECCCCCCEE
IFCFPRTETGQCNACAHSCVGRIRYVGVLLYDAEKVEQALLKPDNQLVEAHRGVILDPRD
EEEECCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHHCCCEECCCH
EAVREGARKNGVPEQWLEAAEKSPVYALIKEFELALPLHPEFRTMPMAYYIPSLSPVLTS
HHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCHHEECCCCCHHHHC
GGELHELVEHGTIPTREALRAPIGYLASLFGGGNRDVVAAALEKLIALRIHMRAKNLNQP
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TDEASLGKAGLDEAGAKRLYRLFTIGGYNERNIIPPQQREELDSHTRKGASGFGILKKTG
CCHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCC
RGKR
CCCC
>Mature Secondary Structure
MDVRAQLVMVFNLDKCIGCHACSLSCKNIWTDRKGAEYMWWNNVETKPGVGYPKKWEDQE
CCCCEEEEEEEECHHHHCCHHHCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
EFKGGWVADGHNLRLKAMGKGPTLLNMFFQPNMPKLEDYYEPFDFDYGNLYGAKGGDDQP
HHCCCEEECCCCEEEEEECCCCCEEEEEECCCCCCHHHHCCCCCCCCCCEECCCCCCCCH
VAEAFSQITGEKMAHIKAGPNWDDDLSGSQVYASADVNLEDKKIIEQYSTMFMQYLPRIC
HHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
NHCANPACVASCPSRAIYKRGEDGVVLVDQEVCKGWRFCTSACPYKKVYFNWNSGKAEKC
HHCCCCCEECCCCCHHHHHCCCCCEEEECHHHHCHHHHHHCCCCCEEEEEEECCCCCCEE
IFCFPRTETGQCNACAHSCVGRIRYVGVLLYDAEKVEQALLKPDNQLVEAHRGVILDPRD
EEEECCCCCCCCHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHHCCCEECCCH
EAVREGARKNGVPEQWLEAAEKSPVYALIKEFELALPLHPEFRTMPMAYYIPSLSPVLTS
HHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCHHEECCCCCHHHHC
GGELHELVEHGTIPTREALRAPIGYLASLFGGGNRDVVAAALEKLIALRIHMRAKNLNQP
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TDEASLGKAGLDEAGAKRLYRLFTIGGYNERNIIPPQQREELDSHTRKGASGFGILKKTG
CCHHHCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCC
RGKR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]