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The map label for this gene is moaA [H]

Identifier: 78221524

GI number: 78221524

Start: 337131

End: 338111

Strand: Direct

Name: moaA [H]

Synonym: Gmet_0301

Alternate gene names: 78221524

Gene position: 337131-338111 (Clockwise)

Preceding gene: 78221523

Following gene: 78221525

Centisome position: 8.43

GC content: 66.16

Gene sequence:

>981_bases
ATGAAACTCGTTGACAGCCACGGGCGGAGGATCAACTATCTCCGCCTCTCGGTCACCGACCGCTGCAACCTCCGCTGCCG
CTACTGCATGCCGGCCGAGGGGGTGGAGATGTTGAGCCACGGCGAAATTCTCTCCTACGAGGAACTCTCCCGGATTGCCC
GGGCCGCGGTGGCCATCGGCATCGAGAAGATCCGGATCACCGGCGGCGAACCCCTGGTCCGCAGGGGGATCGTGCCGTTC
CTGGCCGGAATCGCGGCGCTGCCGGGACTGCGGCAGCTGGTCCTCACCACCAACGGCATCCTCCTGCCGGAGATGGCGGC
GGATCTGCGCCGGGCCGGGGTCCAACGCCTCAACATCAGCCTCGATTCCCTGAAACCGGAGACGTTCCGCGACATCACCC
GCACCGGCAATGTGGAGCGGGTCCTGGCCGGCATCGACGCGGCCGTGGTTGCCGGCTTTCCCCCCCCGAAGATCAACATG
GTGGTGATGCGCGGGGTGAACGACGACGAGGTGGTGGATTTCGCTCGCCTCACCCTCGACCACCCCTACACGGTCCGCTT
CATCGAATACATGCCGGCCACCCGGGAGGAAAACTGGCAACGGCTCTGCATCCCGGCCCAGGAGGTCCTCGACCGGATAG
CCGCCAGCCACGAACTGGAGCCGGTGGAAAGGGGGGCCATGGCCGGCCCGTCGCGGGATTTCCGCATCCGCGGCGCCGTC
GGGACCATCGGGGTCATCTCCGCTGTTTCGGGGCACTTCTGCGGTGAATGCAACCGGATCCGTGTCACCTCCACCGGCAT
GGCCAAGAGTTGCCTCTTTTCCGACGAGGGGCTTGACCTGAAACCGTTTCTGGGAGAGAACGCCCCGCCGGTGCTGGAGG
ATGCTCTGCGGCGGCTGGTGGGATGCAAGCCGGCACGCCATGGGATGGATGGGGAGAAGGTGGAGCATGCGGCCTTCTCC
ATGGCGAAGGTGGGGGGATAA

Upstream 100 bases:

>100_bases
GTCTTTGACCTGAACAATCCCGCCCAGGTGGCCGACTTTGTGGAGCGGACGTTCCTTGCCGCGGGTGCAGCCTGATGGAT
GGCATCTTTCGAGGAAACCG

Downstream 100 bases:

>100_bases
CAGAATTCAGGAGTCAGAATTCAGAATTCAGGAGAAAGGCAGGCTCCATTCCATGCTGACTACTATATTCCGACTTCTGA
CTCCTTCAAGGAGAGGTTCA

Product: radical SAM:molybdenum cofactor synthesis-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKLVDSHGRRINYLRLSVTDRCNLRCRYCMPAEGVEMLSHGEILSYEELSRIARAAVAIGIEKIRITGGEPLVRRGIVPF
LAGIAALPGLRQLVLTTNGILLPEMAADLRRAGVQRLNISLDSLKPETFRDITRTGNVERVLAGIDAAVVAGFPPPKINM
VVMRGVNDDEVVDFARLTLDHPYTVRFIEYMPATREENWQRLCIPAQEVLDRIAASHELEPVERGAMAGPSRDFRIRGAV
GTIGVISAVSGHFCGECNRIRVTSTGMAKSCLFSDEGLDLKPFLGENAPPVLEDALRRLVGCKPARHGMDGEKVEHAAFS
MAKVGG

Sequences:

>Translated_326_residues
MKLVDSHGRRINYLRLSVTDRCNLRCRYCMPAEGVEMLSHGEILSYEELSRIARAAVAIGIEKIRITGGEPLVRRGIVPF
LAGIAALPGLRQLVLTTNGILLPEMAADLRRAGVQRLNISLDSLKPETFRDITRTGNVERVLAGIDAAVVAGFPPPKINM
VVMRGVNDDEVVDFARLTLDHPYTVRFIEYMPATREENWQRLCIPAQEVLDRIAASHELEPVERGAMAGPSRDFRIRGAV
GTIGVISAVSGHFCGECNRIRVTSTGMAKSCLFSDEGLDLKPFLGENAPPVLEDALRRLVGCKPARHGMDGEKVEHAAFS
MAKVGG
>Mature_326_residues
MKLVDSHGRRINYLRLSVTDRCNLRCRYCMPAEGVEMLSHGEILSYEELSRIARAAVAIGIEKIRITGGEPLVRRGIVPF
LAGIAALPGLRQLVLTTNGILLPEMAADLRRAGVQRLNISLDSLKPETFRDITRTGNVERVLAGIDAAVVAGFPPPKINM
VVMRGVNDDEVVDFARLTLDHPYTVRFIEYMPATREENWQRLCIPAQEVLDRIAASHELEPVERGAMAGPSRDFRIRGAV
GTIGVISAVSGHFCGECNRIRVTSTGMAKSCLFSDEGLDLKPFLGENAPPVLEDALRRLVGCKPARHGMDGEKVEHAAFS
MAKVGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=327, Percent_Identity=42.5076452599388, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI22027536, Length=327, Percent_Identity=42.5076452599388, Blast_Score=243, Evalue=2e-64,
Organism=Escherichia coli, GI1786999, Length=295, Percent_Identity=36.6101694915254, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI32566329, Length=314, Percent_Identity=36.9426751592357, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=36.2459546925566, Blast_Score=210, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17561210, Length=314, Percent_Identity=35.3503184713376, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI28574490, Length=318, Percent_Identity=43.3962264150943, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI28574488, Length=320, Percent_Identity=43.125, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35679; Mature: 35679

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVDSHGRRINYLRLSVTDRCNLRCRYCMPAEGVEMLSHGEILSYEELSRIARAAVAIG
CCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCHHHHHCCCEECHHHHHHHHHHHHHHH
IEKIRITGGEPLVRRGIVPFLAGIAALPGLRQLVLTTNGILLPEMAADLRRAGVQRLNIS
HEEEEEECCCHHHHHCHHHHHHHHHHCCHHHHHHHHCCCEECHHHHHHHHHCCHHEEEEE
LDSLKPETFRDITRTGNVERVLAGIDAAVVAGFPPPKINMVVMRGVNDDEVVDFARLTLD
HHHCCCHHHHHHHHCCCHHHHHHCCCHHEECCCCCCCEEEEEEECCCCHHHHHHHHHHCC
HPYTVRFIEYMPATREENWQRLCIPAQEVLDRIAASHELEPVERGAMAGPSRDFRIRGAV
CCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCC
GTIGVISAVSGHFCGECNRIRVTSTGMAKSCLFSDEGLDLKPFLGENAPPVLEDALRRLV
CHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHH
GCKPARHGMDGEKVEHAAFSMAKVGG
CCCCHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLVDSHGRRINYLRLSVTDRCNLRCRYCMPAEGVEMLSHGEILSYEELSRIARAAVAIG
CCCCCCCCCEEEEEEEEECCCCCCEEEEECCCCCHHHHHCCCEECHHHHHHHHHHHHHHH
IEKIRITGGEPLVRRGIVPFLAGIAALPGLRQLVLTTNGILLPEMAADLRRAGVQRLNIS
HEEEEEECCCHHHHHCHHHHHHHHHHCCHHHHHHHHCCCEECHHHHHHHHHCCHHEEEEE
LDSLKPETFRDITRTGNVERVLAGIDAAVVAGFPPPKINMVVMRGVNDDEVVDFARLTLD
HHHCCCHHHHHHHHCCCHHHHHHCCCHHEECCCCCCCEEEEEEECCCCHHHHHHHHHHCC
HPYTVRFIEYMPATREENWQRLCIPAQEVLDRIAASHELEPVERGAMAGPSRDFRIRGAV
CCCEEEEEECCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCC
GTIGVISAVSGHFCGECNRIRVTSTGMAKSCLFSDEGLDLKPFLGENAPPVLEDALRRLV
CHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHH
GCKPARHGMDGEKVEHAAFSMAKVGG
CCCCHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA