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The map label for this gene is ltaA [H]

Identifier: 78221493

GI number: 78221493

Start: 299885

End: 300904

Strand: Direct

Name: ltaA [H]

Synonym: Gmet_0270

Alternate gene names: 78221493

Gene position: 299885-300904 (Clockwise)

Preceding gene: 78221492

Following gene: 78221494

Centisome position: 7.5

GC content: 67.94

Gene sequence:

>1020_bases
ATGAAGATCATCGACCTGCGGAGCGATACCGTCACCAGGCCCTGCCCGGCCATGCGGGCCGCTATGGCTGTAGCCGAGGT
GGGTGACGACGTCTATGGCGAGGACCCCACCGTGAACCGCCTCGAAGCCCTGGCCGCCGGGATGCTCGGCACCGAGGCGG
CCCTCTTCGTGACGAGCGGCACCCAGTCCAACCTCCTTGCCATCATGACCCACTGCGGGCGGGGTGACGAGTACATCGCC
GGCCAGACGGCCCACTGCTACCGTTTCGAGGGGGGCGGCGCCGCGGTCCTCGGCGGGGTCCAGCCCCAGCCCGTGGACGT
GGAACCCGACGGAACCCTTGACCTCGTGAAAGTGGCCGCGGCCATCAAGCCCAACGATCCCCACTTTGCCCGGACACGGC
TCCTCTGTCTGGAGAACACCCAGGCGGGGAGGGTTCTCCCCCTCGATTACCTGGCTCGCGCCCGTACCCTTGCCCGGGAG
CGTGGCCTCGCCCTCCACCTGGACGGGGCGCGGCTCTTCAATGCCGCCGTGGCCCTCGACGTGGAAGCCCGGGAGATAAC
GGATCATTTCGAATCGGTGTCGGTCTGTCTCTCCAAGGGGCTCGGCGCGCCGGTGGGGTCGCTTTTGTGCGGGAGTCGGG
GGTTTGTGGCCGAGGCCCGACGCTGGCGCAAGGTCCTCGGCGGCGGCATGCGCCAGGCAGGTGTCCTGGCCGCCGCGGGA
ATTGTGGCCCTCACGGAGAACGTGGCGCGCCTCGCCGAGGACCACGCCAATGCGCGGCGTCTGGCCGAGGGGCTGGCTGC
CGTCGGCGGTCTCGATGGTGACTCCGTCTCGGTTCAGACGAACATGGTGTTCCTCTCGCTTCCCCCTGATCATGCCGAGG
CACTGGCAACCTTTCTCTGCGACAGGGGAGTTCTCATCTCCGGTCGGGAGCGCATCCGCCTTGTCACCCATCGGGATGTG
ACCTCTGCCGACATCGATCGGGTAGTGGGAGCCTGCGGGGAGTTTTTCAGGCGACCCTGA

Upstream 100 bases:

>100_bases
GAGAGCGTCATGATCGGCGCCCGGTCGGGCCAGGGAATCTCCTTCGTGAGCCCGGCCCGGGAGATCCGGCCCCAGGCCAA
ATGAGGATTGAGGTGCCACG

Downstream 100 bases:

>100_bases
CGCCTCATTCCTTCCATTGCAAAAGCTGCCTCCTATGGTTTACTTGAACGTGTCGCCTCCCCGAAAAACGCCTTCTGGAG
TCAGATCATGCCCATGAAAA

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MKIIDLRSDTVTRPCPAMRAAMAVAEVGDDVYGEDPTVNRLEALAAGMLGTEAALFVTSGTQSNLLAIMTHCGRGDEYIA
GQTAHCYRFEGGGAAVLGGVQPQPVDVEPDGTLDLVKVAAAIKPNDPHFARTRLLCLENTQAGRVLPLDYLARARTLARE
RGLALHLDGARLFNAAVALDVEAREITDHFESVSVCLSKGLGAPVGSLLCGSRGFVAEARRWRKVLGGGMRQAGVLAAAG
IVALTENVARLAEDHANARRLAEGLAAVGGLDGDSVSVQTNMVFLSLPPDHAEALATFLCDRGVLISGRERIRLVTHRDV
TSADIDRVVGACGEFFRRP

Sequences:

>Translated_339_residues
MKIIDLRSDTVTRPCPAMRAAMAVAEVGDDVYGEDPTVNRLEALAAGMLGTEAALFVTSGTQSNLLAIMTHCGRGDEYIA
GQTAHCYRFEGGGAAVLGGVQPQPVDVEPDGTLDLVKVAAAIKPNDPHFARTRLLCLENTQAGRVLPLDYLARARTLARE
RGLALHLDGARLFNAAVALDVEAREITDHFESVSVCLSKGLGAPVGSLLCGSRGFVAEARRWRKVLGGGMRQAGVLAAAG
IVALTENVARLAEDHANARRLAEGLAAVGGLDGDSVSVQTNMVFLSLPPDHAEALATFLCDRGVLISGRERIRLVTHRDV
TSADIDRVVGACGEFFRRP
>Mature_339_residues
MKIIDLRSDTVTRPCPAMRAAMAVAEVGDDVYGEDPTVNRLEALAAGMLGTEAALFVTSGTQSNLLAIMTHCGRGDEYIA
GQTAHCYRFEGGGAAVLGGVQPQPVDVEPDGTLDLVKVAAAIKPNDPHFARTRLLCLENTQAGRVLPLDYLARARTLARE
RGLALHLDGARLFNAAVALDVEAREITDHFESVSVCLSKGLGAPVGSLLCGSRGFVAEARRWRKVLGGGMRQAGVLAAAG
IVALTENVARLAEDHANARRLAEGLAAVGGLDGDSVSVQTNMVFLSLPPDHAEALATFLCDRGVLISGRERIRLVTHRDV
TSADIDRVVGACGEFFRRP

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=336, Percent_Identity=56.547619047619, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI193206522, Length=350, Percent_Identity=42.2857142857143, Blast_Score=252, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI193206524, Length=350, Percent_Identity=42.2857142857143, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI193206520, Length=350, Percent_Identity=42.2857142857143, Blast_Score=251, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6320789, Length=342, Percent_Identity=39.4736842105263, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI21355439, Length=298, Percent_Identity=49.3288590604027, Blast_Score=261, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 35858; Mature: 35858

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIDLRSDTVTRPCPAMRAAMAVAEVGDDVYGEDPTVNRLEALAAGMLGTEAALFVTSG
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
TQSNLLAIMTHCGRGDEYIAGQTAHCYRFEGGGAAVLGGVQPQPVDVEPDGTLDLVKVAA
CCCCHHHHHHHCCCCCCEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
AIKPNDPHFARTRLLCLENTQAGRVLPLDYLARARTLARERGLALHLDGARLFNAAVALD
HCCCCCCCHHHEEEEEEECCCCCCEECHHHHHHHHHHHHHCCCEEEECCHHHHHEEEEEE
VEAREITDHFESVSVCLSKGLGAPVGSLLCGSRGFVAEARRWRKVLGGGMRQAGVLAAAG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
IVALTENVARLAEDHANARRLAEGLAAVGGLDGDSVSVQTNMVFLSLPPDHAEALATFLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
DRGVLISGRERIRLVTHRDVTSADIDRVVGACGEFFRRP
CCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIDLRSDTVTRPCPAMRAAMAVAEVGDDVYGEDPTVNRLEALAAGMLGTEAALFVTSG
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
TQSNLLAIMTHCGRGDEYIAGQTAHCYRFEGGGAAVLGGVQPQPVDVEPDGTLDLVKVAA
CCCCHHHHHHHCCCCCCEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHH
AIKPNDPHFARTRLLCLENTQAGRVLPLDYLARARTLARERGLALHLDGARLFNAAVALD
HCCCCCCCHHHEEEEEEECCCCCCEECHHHHHHHHHHHHHCCCEEEECCHHHHHEEEEEE
VEAREITDHFESVSVCLSKGLGAPVGSLLCGSRGFVAEARRWRKVLGGGMRQAGVLAAAG
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
IVALTENVARLAEDHANARRLAEGLAAVGGLDGDSVSVQTNMVFLSLPPDHAEALATFLC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
DRGVLISGRERIRLVTHRDVTSADIDRVVGACGEFFRRP
CCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]