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The map label for this gene is truC [H]

Identifier: 78221490

GI number: 78221490

Start: 298034

End: 298846

Strand: Direct

Name: truC [H]

Synonym: Gmet_0267

Alternate gene names: 78221490

Gene position: 298034-298846 (Clockwise)

Preceding gene: 78221488

Following gene: 78221491

Centisome position: 7.46

GC content: 63.59

Gene sequence:

>813_bases
GTGGAAATCCTTTACCGCGACGAGCATCTCGTGGCAGTCCACAAACCGCTGGGACTACTCGTCCACCGCTCGCCCATAGA
CCGGCACGAGACCCGGTTTGCCCTCCAGGAAGTGCGTGACCTTCTGGGGCGGCGCGTCTATCCGGTTCACCGACTCGACA
AACCCACCTCAGGTGTCCTCCTCTTCGCCCTCGATCCAGCTTTCGCCCGGGGCCTCGTCGGCGCCTTCGCGTCCGGGAGC
GTGACCAAGACGTATCTGGCGGTGGTTCGGGGAACCATGCCGGAGGAAGGGGTGATTGACCATCCCCTGGCAGAGGAGCC
CGATCGTTTCGAGCAGGGACGGGGGAATGCCGGAGAGAGGGCGCCCCAGTCGGCAGTGACCGGCTATCGACGCTTGGCAG
ACGTGGAGCTTCCTGTTGCCGTTGGACGCTATCCCACGAGTCGCTATTCGCTGGTGTCGCTCTCGCCGCGCACGGGTCGC
CGTCATCAGCTTCGCCGCCACCTCAAGCACCTCTTCCACCCCATCGTTGGCGATACGAAATACGGCGAAGGGCGCCACAA
CCGGTTCTTTCGGGAAGAGCTTCTGACGGTACGCATGCTTCTGCACGCCGTTGAGCTTACCGTTCCTCACCCGGCAACCG
GTGCGCCGCTGACGGTCTGTGCCCCGGTCGAGGGGGCTTTTGCAGCTCTCCTCGCCCGGTTCGGCTGGCTCTCTGCCGTT
CCTTCCCGTTGGCTCGGCCCCCAGGGAAAAAGCATGGCTGGGGAGGAGATGCAAACGAGACAGCGGATTTCTCCTGTGCT
ACCTTTGTTGTAG

Upstream 100 bases:

>100_bases
CATTTTTGAGAATCAATAAGCCTCTAAACTATAACGCAAATACTTTTCATTTGCAGGTTTTTTTTTCGCGGAAAAAACGA
TGCTATCTTCAGGAGCCACC

Downstream 100 bases:

>100_bases
TATCGATACAGTGTTGACAGGGAGGTTCACATCATGGACGAAACACCAGAACTTGAAAAGGCAACCTTCGGTGCCGGGTG
CTTCTGGCACGTGGAGGCCG

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MEILYRDEHLVAVHKPLGLLVHRSPIDRHETRFALQEVRDLLGRRVYPVHRLDKPTSGVLLFALDPAFARGLVGAFASGS
VTKTYLAVVRGTMPEEGVIDHPLAEEPDRFEQGRGNAGERAPQSAVTGYRRLADVELPVAVGRYPTSRYSLVSLSPRTGR
RHQLRRHLKHLFHPIVGDTKYGEGRHNRFFREELLTVRMLLHAVELTVPHPATGAPLTVCAPVEGAFAALLARFGWLSAV
PSRWLGPQGKSMAGEEMQTRQRISPVLPLL

Sequences:

>Translated_270_residues
MEILYRDEHLVAVHKPLGLLVHRSPIDRHETRFALQEVRDLLGRRVYPVHRLDKPTSGVLLFALDPAFARGLVGAFASGS
VTKTYLAVVRGTMPEEGVIDHPLAEEPDRFEQGRGNAGERAPQSAVTGYRRLADVELPVAVGRYPTSRYSLVSLSPRTGR
RHQLRRHLKHLFHPIVGDTKYGEGRHNRFFREELLTVRMLLHAVELTVPHPATGAPLTVCAPVEGAFAALLARFGWLSAV
PSRWLGPQGKSMAGEEMQTRQRISPVLPLL
>Mature_270_residues
MEILYRDEHLVAVHKPLGLLVHRSPIDRHETRFALQEVRDLLGRRVYPVHRLDKPTSGVLLFALDPAFARGLVGAFASGS
VTKTYLAVVRGTMPEEGVIDHPLAEEPDRFEQGRGNAGERAPQSAVTGYRRLADVELPVAVGRYPTSRYSLVSLSPRTGR
RHQLRRHLKHLFHPIVGDTKYGEGRHNRFFREELLTVRMLLHAVELTVPHPATGAPLTVCAPVEGAFAALLARFGWLSAV
PSRWLGPQGKSMAGEEMQTRQRISPVLPLL

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI221316663, Length=210, Percent_Identity=33.8095238095238, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI221316667, Length=202, Percent_Identity=34.1584158415842, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI17158025, Length=240, Percent_Identity=32.5, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI23308689, Length=186, Percent_Identity=31.1827956989247, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789155, Length=243, Percent_Identity=50.6172839506173, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1786244, Length=230, Percent_Identity=36.5217391304348, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788946, Length=240, Percent_Identity=28.75, Blast_Score=78, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320168, Length=185, Percent_Identity=30.2702702702703, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321608, Length=195, Percent_Identity=31.7948717948718, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324506, Length=187, Percent_Identity=29.9465240641711, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583449, Length=183, Percent_Identity=30.6010928961749, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583447, Length=183, Percent_Identity=30.6010928961749, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583445, Length=183, Percent_Identity=30.6010928961749, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921090, Length=183, Percent_Identity=30.6010928961749, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 30096; Mature: 30096

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILYRDEHLVAVHKPLGLLVHRSPIDRHETRFALQEVRDLLGRRVYPVHRLDKPTSGVL
CCEEEECCEEEEEECCHHHEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEE
LFALDPAFARGLVGAFASGSVTKTYLAVVRGTMPEEGVIDHPLAEEPDRFEQGRGNAGER
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
APQSAVTGYRRLADVELPVAVGRYPTSRYSLVSLSPRTGRRHQLRRHLKHLFHPIVGDTK
CHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
YGEGRHNRFFREELLTVRMLLHAVELTVPHPATGAPLTVCAPVEGAFAALLARFGWLSAV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
PSRWLGPQGKSMAGEEMQTRQRISPVLPLL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEILYRDEHLVAVHKPLGLLVHRSPIDRHETRFALQEVRDLLGRRVYPVHRLDKPTSGVL
CCEEEECCEEEEEECCHHHEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEE
LFALDPAFARGLVGAFASGSVTKTYLAVVRGTMPEEGVIDHPLAEEPDRFEQGRGNAGER
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
APQSAVTGYRRLADVELPVAVGRYPTSRYSLVSLSPRTGRRHQLRRHLKHLFHPIVGDTK
CHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCC
YGEGRHNRFFREELLTVRMLLHAVELTVPHPATGAPLTVCAPVEGAFAALLARFGWLSAV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
PSRWLGPQGKSMAGEEMQTRQRISPVLPLL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]