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The map label for this gene is pilT [H]

Identifier: 78221483

GI number: 78221483

Start: 291318

End: 292388

Strand: Reverse

Name: pilT [H]

Synonym: Gmet_0260

Alternate gene names: 78221483

Gene position: 292388-291318 (Counterclockwise)

Preceding gene: 78221484

Following gene: 78221482

Centisome position: 7.31

GC content: 63.03

Gene sequence:

>1071_bases
ATGGATATGAACCTTCTGAACCAGATACTGGGTATCGCCTTCGAGAAGCGGGTGTCGGATCTCCATTTCGAGGTGGACAA
TCCCCCCTTCTTCCGGGCCAAGGGGCAACTGCTCCGCTCCAAGCTCCCCCGGCTGACCCCCCAGGACACGGAATTCATCG
CCAAAACCGTCCTGGAGCAGAACCGGCGCACTTTCCCCGACAATCTGCGGGAATTGGACGCCTCTTATTCCCTCCCCAAC
GGCGGCCGCTTCCGGGTCAGCATCTTCCGCCAGCGGGGGGCCATCGGCATCGTCATGCGGGTGATCCCTCCCCATGTGGG
GAGCTTCGAGGAACTGAACCTGCCGCCGGTCCTAGGGGAGATCGCCAAGGCCCCCAACGGGCTCGTGCTGGTCACCGGTC
CCACCGGCAACGGCAAGTCCACGACCCTTGCCTCCATGCTCCGCCACCTGAACGAGACGTGCTCCTTCAACATCATCACC
ATCGAGGATCCCATCGAATTCCTCTTCACCTCTGACCGGAGCTGCATCATCCAGCGGGAGGTGGGGATCGACACGGAAAA
CTTCAGCGCGGCGCTTCGCTCGGCGCTGCGGATGGACCCCGACGTCATCATGGTGGGGGAGATGCGGGACCTGGAGACCA
TCGACTCCTGCATCAAGGCGGCGGAAACGGGGCACCTGGTCTTCTCCACCCTCCACACCCAGAGCGCCGTCTCCACCATC
AACCGGCTCATCGGCCACTTCCCCCCCGACGCCCAGGAGGTCCTGCGCCAGCGCCTGGCCGACATCCTGGTGGCCACGGT
GTCGTTGCGGCTCATCAAGGACAAGAGCGGCGAGAACATCCTCCCGGTGGTGGAGATCATGCGGGCCACCACCACCATCC
AGGCATGCATCCGCGAGGGGCGCCTCGACGAGATCGAGAAGCACATCGAGAACGGCCGTTCCCTCTACCACATGCAGACC
CTGGACCAGCACCTCCTGGAGCTCTGCGAGAAGGAGATCATCACCTTCGATCAGGCCAAGCAGATCACCCGCTCCATGGA
CCTGGAACGGAAACTGGCCTTCACCGAATGA

Upstream 100 bases:

>100_bases
CTGGCCGGGGAGGCAGGGATCGTGCTGGAAAACGCCGTCTACCGCCGAAAGCGTGAAAAGCCGCACCCCTGATGGAACGA
GACGTTAGCGAGGTACTTTC

Downstream 100 bases:

>100_bases
TGGGAGAGGGATAAAGATATTTTCACTGGACTGCAGGGCGCAAGACCGCGTACGATGGCGGAATCGGCCACGGGCCGGAT
ATATCATAAGGGTACGGAAG

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDMNLLNQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPRLTPQDTEFIAKTVLEQNRRTFPDNLRELDASYSLPN
GGRFRVSIFRQRGAIGIVMRVIPPHVGSFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMLRHLNETCSFNIIT
IEDPIEFLFTSDRSCIIQREVGIDTENFSAALRSALRMDPDVIMVGEMRDLETIDSCIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEIMRATTTIQACIREGRLDEIEKHIENGRSLYHMQT
LDQHLLELCEKEIITFDQAKQITRSMDLERKLAFTE

Sequences:

>Translated_356_residues
MDMNLLNQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPRLTPQDTEFIAKTVLEQNRRTFPDNLRELDASYSLPN
GGRFRVSIFRQRGAIGIVMRVIPPHVGSFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMLRHLNETCSFNIIT
IEDPIEFLFTSDRSCIIQREVGIDTENFSAALRSALRMDPDVIMVGEMRDLETIDSCIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEIMRATTTIQACIREGRLDEIEKHIENGRSLYHMQT
LDQHLLELCEKEIITFDQAKQITRSMDLERKLAFTE
>Mature_356_residues
MDMNLLNQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPRLTPQDTEFIAKTVLEQNRRTFPDNLRELDASYSLPN
GGRFRVSIFRQRGAIGIVMRVIPPHVGSFEELNLPPVLGEIAKAPNGLVLVTGPTGNGKSTTLASMLRHLNETCSFNIIT
IEDPIEFLFTSDRSCIIQREVGIDTENFSAALRSALRMDPDVIMVGEMRDLETIDSCIKAAETGHLVFSTLHTQSAVSTI
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEIMRATTTIQACIREGRLDEIEKHIENGRSLYHMQT
LDQHLLELCEKEIITFDQAKQITRSMDLERKLAFTE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=313, Percent_Identity=39.9361022364217, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1786296, Length=289, Percent_Identity=37.0242214532872, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789723, Length=271, Percent_Identity=33.5793357933579, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40085; Mature: 40085

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMNLLNQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPRLTPQDTEFIAKTVLEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NRRTFPDNLRELDASYSLPNGGRFRVSIFRQRGAIGIVMRVIPPHVGSFEELNLPPVLGE
CCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHH
IAKAPNGLVLVTGPTGNGKSTTLASMLRHLNETCSFNIITIEDPIEFLFTSDRSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHCCCCCEEEEEC
VGIDTENFSAALRSALRMDPDVIMVGEMRDLETIDSCIKAAETGHLVFSTLHTQSAVSTI
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEIMRATTTIQACIREG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RLDEIEKHIENGRSLYHMQTLDQHLLELCEKEIITFDQAKQITRSMDLERKLAFTE
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC
>Mature Secondary Structure
MDMNLLNQILGIAFEKRVSDLHFEVDNPPFFRAKGQLLRSKLPRLTPQDTEFIAKTVLEQ
CCHHHHHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NRRTFPDNLRELDASYSLPNGGRFRVSIFRQRGAIGIVMRVIPPHVGSFEELNLPPVLGE
CCCCCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHHHH
IAKAPNGLVLVTGPTGNGKSTTLASMLRHLNETCSFNIITIEDPIEFLFTSDRSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHHHHCCCCCEEEEEC
VGIDTENFSAALRSALRMDPDVIMVGEMRDLETIDSCIKAAETGHLVFSTLHTQSAVSTI
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NRLIGHFPPDAQEVLRQRLADILVATVSLRLIKDKSGENILPVVEIMRATTTIQACIREG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHCC
RLDEIEKHIENGRSLYHMQTLDQHLLELCEKEIITFDQAKQITRSMDLERKLAFTE
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]