Click here to switch to the map view.

The map label for this gene is pilT [H]

Identifier: 78221423

GI number: 78221423

Start: 230732

End: 231895

Strand: Direct

Name: pilT [H]

Synonym: Gmet_0199

Alternate gene names: 78221423

Gene position: 230732-231895 (Clockwise)

Preceding gene: 78221422

Following gene: 78221424

Centisome position: 5.77

GC content: 61.86

Gene sequence:

>1164_bases
ATGGAACTCAATGATATTCTTGCCATAGCGGTCAAGGCTAAAGCGTCTGATATCCACATCAAGACGGGGCTCCCCCCGGT
TGTCCGGATCGATGGACGGCTGCGGCCCATTCCCAATGCACAGCGTCTTGCCCCGGACCAGGTTCGCGCCATGGCCTTTG
CCATCATGAATGAGCGACAAAAGGGGATCTTCGAGGAGCACTACGAGTGCGACGTGGCCTACGGGGTGCCGGGACTCGGC
CGCTTCCGGGTGAGTATCTATTCCCAGCGGGGGACCGTGGCCATGGTCTTCCGGTCCATCCCCTTCGGCATCCCCTCCAT
TGAGAACCTGACCCTTCCGCCGGTCATCAAGAAGCTTGCACTGGAGGAACGTGGGCTCATCCTCGTCACCGGCACCACGG
GAAGCGGCAAGTCCACGACCCTGGCCGCCATGATCGACTACATCAACGAGCACCGGACCTGCAACATCATCACCGTCGAA
GACCCGGTGGAGTTCCTGCATCGGGACAAGAAGAGCATCCTCTCCCAGCGGGAGGTGGGGTTCGATACCCTTTCCTTCTC
CACTGCCCTGAAGGGAGCCCTACGCCAGGACCCGGACGTGGTTCTCGTGGGGGAGATGCGGGACCTGGAAACCATCGAGA
CCGCCATGCACGCCGCCGAGACCGGGCACCTGGTCATGTCCACCCTCCATACCCTGGACGCCGCCGAAACCATCAACCGG
ATCATCTCCGTCTTCCCTCCCTTCCACCAGCGCCAGGTCAGGCTCCAACTCTCCGGGGTCATCAAGGGGGTCATATCCCA
GCGGCTGGTCCCCCGGGCCGACGGCAAGGGGCGGGTTCCGGCCGTGGAGGTCATGATCGGCACCGCCCGCATCAAGGAAT
ATATCGACGACAAGGACAAGACGAAGCTCCTCCCCGAGGCCATCGCCCAAGGGTTCACCACCTACGGGATGCAGACATTT
GACCAGTCCCTCATGCAGCTCTACACCGGCAAGCTCATCACCTACGAGGAAGCGCTCCGGCAGTCCACCAACCCGGATGA
TTTCGCCCTCAAGGTGTCGGGCATCTCTTCCACGTCCGACAGCACGTGGGACAACTTTGTCCACGACGAAGCCCCCCCCG
CGGCTGCTGGAGATACGCCCACCGAGGGGATCGAGAAGTTTTAG

Upstream 100 bases:

>100_bases
CCTCAAGGAGCATCCGGAGATGGTGGCCGAAATCGAGGACAAGATTTACGAAACGGCCGGCATTCCCCGCAAAGGGGGGA
AGGAAGAGGCGGCGTAGCCC

Downstream 100 bases:

>100_bases
ATGGGGGAGGAGCGGAGCGGGTTCAGCCGCGCCCTTGATATTCTCTCCCGGCGTGACCACAGCGAGGCGGAGTTGGCCCT
GAAGCTGCGGCGCAAGGGGA

Product: pilus retraction protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MELNDILAIAVKAKASDIHIKTGLPPVVRIDGRLRPIPNAQRLAPDQVRAMAFAIMNERQKGIFEEHYECDVAYGVPGLG
RFRVSIYSQRGTVAMVFRSIPFGIPSIENLTLPPVIKKLALEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTLSFSTALKGALRQDPDVVLVGEMRDLETIETAMHAAETGHLVMSTLHTLDAAETINR
IISVFPPFHQRQVRLQLSGVIKGVISQRLVPRADGKGRVPAVEVMIGTARIKEYIDDKDKTKLLPEAIAQGFTTYGMQTF
DQSLMQLYTGKLITYEEALRQSTNPDDFALKVSGISSTSDSTWDNFVHDEAPPAAAGDTPTEGIEKF

Sequences:

>Translated_387_residues
MELNDILAIAVKAKASDIHIKTGLPPVVRIDGRLRPIPNAQRLAPDQVRAMAFAIMNERQKGIFEEHYECDVAYGVPGLG
RFRVSIYSQRGTVAMVFRSIPFGIPSIENLTLPPVIKKLALEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTLSFSTALKGALRQDPDVVLVGEMRDLETIETAMHAAETGHLVMSTLHTLDAAETINR
IISVFPPFHQRQVRLQLSGVIKGVISQRLVPRADGKGRVPAVEVMIGTARIKEYIDDKDKTKLLPEAIAQGFTTYGMQTF
DQSLMQLYTGKLITYEEALRQSTNPDDFALKVSGISSTSDSTWDNFVHDEAPPAAAGDTPTEGIEKF
>Mature_387_residues
MELNDILAIAVKAKASDIHIKTGLPPVVRIDGRLRPIPNAQRLAPDQVRAMAFAIMNERQKGIFEEHYECDVAYGVPGLG
RFRVSIYSQRGTVAMVFRSIPFGIPSIENLTLPPVIKKLALEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTLSFSTALKGALRQDPDVVLVGEMRDLETIETAMHAAETGHLVMSTLHTLDAAETINR
IISVFPPFHQRQVRLQLSGVIKGVISQRLVPRADGKGRVPAVEVMIGTARIKEYIDDKDKTKLLPEAIAQGFTTYGMQTF
DQSLMQLYTGKLITYEEALRQSTNPDDFALKVSGISSTSDSTWDNFVHDEAPPAAAGDTPTEGIEKF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=323, Percent_Identity=41.1764705882353, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1789723, Length=301, Percent_Identity=35.8803986710963, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786296, Length=283, Percent_Identity=35.6890459363958, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42659; Mature: 42659

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNDILAIAVKAKASDIHIKTGLPPVVRIDGRLRPIPNAQRLAPDQVRAMAFAIMNERQ
CCCHHEEEEEEEECCCEEEEECCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
KGIFEEHYECDVAYGVPGLGRFRVSIYSQRGTVAMVFRSIPFGIPSIENLTLPPVIKKLA
CCHHHHHCCEEEEECCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCHHHHHHH
LEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVEDPVEFLHRDKKSILSQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTLSFSTALKGALRQDPDVVLVGEMRDLETIETAMHAAETGHLVMSTLHTLDAAETINR
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPFHQRQVRLQLSGVIKGVISQRLVPRADGKGRVPAVEVMIGTARIKEYIDDKDK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCHH
TKLLPEAIAQGFTTYGMQTFDQSLMQLYTGKLITYEEALRQSTNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCEEEEEECCCCCCC
STWDNFVHDEAPPAAAGDTPTEGIEKF
CCHHHHHCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MELNDILAIAVKAKASDIHIKTGLPPVVRIDGRLRPIPNAQRLAPDQVRAMAFAIMNERQ
CCCHHEEEEEEEECCCEEEEECCCCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
KGIFEEHYECDVAYGVPGLGRFRVSIYSQRGTVAMVFRSIPFGIPSIENLTLPPVIKKLA
CCHHHHHCCEEEEECCCCCCEEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCHHHHHHH
LEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVEDPVEFLHRDKKSILSQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTLSFSTALKGALRQDPDVVLVGEMRDLETIETAMHAAETGHLVMSTLHTLDAAETINR
CCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPFHQRQVRLQLSGVIKGVISQRLVPRADGKGRVPAVEVMIGTARIKEYIDDKDK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHCCCHH
TKLLPEAIAQGFTTYGMQTFDQSLMQLYTGKLITYEEALRQSTNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHCCCCCCCEEEEEECCCCCCC
STWDNFVHDEAPPAAAGDTPTEGIEKF
CCHHHHHCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]