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The map label for this gene is ghrA [H]

Identifier: 78221333

GI number: 78221333

Start: 132351

End: 133295

Strand: Reverse

Name: ghrA [H]

Synonym: Gmet_0106

Alternate gene names: 78221333

Gene position: 133295-132351 (Counterclockwise)

Preceding gene: 78221334

Following gene: 78221332

Centisome position: 3.33

GC content: 60.95

Gene sequence:

>945_bases
ATGAAGATCAGCAATCTCCTTATCCATCTGCACAACAACATTGACGCCTTCACCCTGAAGCCACGTCACGTCGAGCAGAT
ACGCCAAGCACTCCCGGAAGTGGCCATTACCGTCTCGGCGAGCGACAGCGACTTCATGGACCGGCTTCCCGATGCCGAAT
ATGCCCTTGTGTGGGTCTTCAAACCCGAGTGGTATGCGACCGCTCCCCGGCTTAAGGCACTCTTCACTCCTGCCGCCGGC
CGTGACTGGGTTGCGGCAGACCCGTCAGGGAGGGTGATGACCTGTCATGGCAGCTTCCACGGTCGTATCATGCGCGAGAG
TCTTCTCTCCATGATGCTTTATTTCAACCGGCGCCTCGGCCAGTCGCTTGAAGATCAGGGCCGCCGGATGTGGGGGCGTC
CGGACTACAGCGGTTGTGTCGGCCTCTTCAGCCAGCGGGTCATGATCGTAGGTTTGGGGGCGCTGGGGCAGTCCATGGCG
GAACTGCTCAAAGCCTTCGGTGCGCACGTAACCGGGGTCAAGCGCAACCCGGAATCCTTTGCGGGCAGCCCTTGTGTCGA
CAGGGTGATCTCTTTTGACGCGCTGGAGGAGGCGTTGCCTGAAGCCGATCATGTGGTTCTGCTGCTTCCCGGCGGGACGG
CAACCGATGGTATTTTCACCGCACGACACTTCAATGCCATGAGGCCGGGAGCCTACCTTTACAACCTGGGTCGAGGGAAC
TGTTACCGGGAGGAAGACCTTGTGGCCGCCCTGGAGGATGGCCGCCTGGCAGGTGCCGGTCTGGATGTCTTTGCCGAAGA
GCCGCTGCCGGCCTCCTCGCCTCTCTGGGAGCAACGCAACGTCCTGATCACGCCCCATTCCAGCGCCATCAGCCGGGAGT
ACCTCGACCTCTTTATTCAGGAGTGGCTAGAGACCCTGCGGAAGGGTGCGCCGCAGAACGCGTAA

Upstream 100 bases:

>100_bases
ATTTGCCGCAGCAGATTGACCACGCCTGCCAGAATCGATATAGTCGTTACTCCTGTAACGCATTGATAAGACCGACGGTT
TCTGAGAGCGAAACGCACCC

Downstream 100 bases:

>100_bases
AGCAACACGTTACAAGCAAGAGGAGTAGATTTATTTCAGAGGAGAACTGCCATGCGCGACTTTTGTGATGCAGACCGGAA
GAAAAAGAGCGGCAGGATCA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKISNLLIHLHNNIDAFTLKPRHVEQIRQALPEVAITVSASDSDFMDRLPDAEYALVWVFKPEWYATAPRLKALFTPAAG
RDWVAADPSGRVMTCHGSFHGRIMRESLLSMMLYFNRRLGQSLEDQGRRMWGRPDYSGCVGLFSQRVMIVGLGALGQSMA
ELLKAFGAHVTGVKRNPESFAGSPCVDRVISFDALEEALPEADHVVLLLPGGTATDGIFTARHFNAMRPGAYLYNLGRGN
CYREEDLVAALEDGRLAGAGLDVFAEEPLPASSPLWEQRNVLITPHSSAISREYLDLFIQEWLETLRKGAPQNA

Sequences:

>Translated_314_residues
MKISNLLIHLHNNIDAFTLKPRHVEQIRQALPEVAITVSASDSDFMDRLPDAEYALVWVFKPEWYATAPRLKALFTPAAG
RDWVAADPSGRVMTCHGSFHGRIMRESLLSMMLYFNRRLGQSLEDQGRRMWGRPDYSGCVGLFSQRVMIVGLGALGQSMA
ELLKAFGAHVTGVKRNPESFAGSPCVDRVISFDALEEALPEADHVVLLLPGGTATDGIFTARHFNAMRPGAYLYNLGRGN
CYREEDLVAALEDGRLAGAGLDVFAEEPLPASSPLWEQRNVLITPHSSAISREYLDLFIQEWLETLRKGAPQNA
>Mature_314_residues
MKISNLLIHLHNNIDAFTLKPRHVEQIRQALPEVAITVSASDSDFMDRLPDAEYALVWVFKPEWYATAPRLKALFTPAAG
RDWVAADPSGRVMTCHGSFHGRIMRESLLSMMLYFNRRLGQSLEDQGRRMWGRPDYSGCVGLFSQRVMIVGLGALGQSMA
ELLKAFGAHVTGVKRNPESFAGSPCVDRVISFDALEEALPEADHVVLLLPGGTATDGIFTARHFNAMRPGAYLYNLGRGN
CYREEDLVAALEDGRLAGAGLDVFAEEPLPASSPLWEQRNVLITPHSSAISREYLDLFIQEWLETLRKGAPQNA

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=186, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI23308577, Length=194, Percent_Identity=29.8969072164948, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI61743967, Length=199, Percent_Identity=31.6582914572864, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557497, Length=199, Percent_Identity=31.6582914572864, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87081824, Length=288, Percent_Identity=32.2916666666667, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87082289, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789279, Length=153, Percent_Identity=30.718954248366, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=278, Percent_Identity=24.8201438848921, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=171, Percent_Identity=28.6549707602339, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320925, Length=206, Percent_Identity=26.2135922330097, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=199, Percent_Identity=26.6331658291457, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552429, Length=240, Percent_Identity=27.9166666666667, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574286, Length=253, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585514, Length=240, Percent_Identity=27.9166666666667, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574282, Length=240, Percent_Identity=27.9166666666667, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571528, Length=175, Percent_Identity=33.1428571428571, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45551003, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574284, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24585516, Length=217, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921140, Length=178, Percent_Identity=30.8988764044944, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646446, Length=280, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646448, Length=280, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646452, Length=280, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646450, Length=280, Percent_Identity=25.7142857142857, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI62472511, Length=280, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34812; Mature: 34812

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISNLLIHLHNNIDAFTLKPRHVEQIRQALPEVAITVSASDSDFMDRLPDAEYALVWVF
CCHHHEEEEEECCCCEEEECHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEEE
KPEWYATAPRLKALFTPAAGRDWVAADPSGRVMTCHGSFHGRIMRESLLSMMLYFNRRLG
CCCHHCCCCHHHHEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHC
QSLEDQGRRMWGRPDYSGCVGLFSQRVMIVGLGALGQSMAELLKAFGAHVTGVKRNPESF
CCHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEECCCCCCHHC
AGSPCVDRVISFDALEEALPEADHVVLLLPGGTATDGIFTARHFNAMRPGAYLYNLGRGN
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEHHHHCCCCCCCEEEECCCCC
CYREEDLVAALEDGRLAGAGLDVFAEEPLPASSPLWEQRNVLITPHSSAISREYLDLFIQ
CCCHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
EWLETLRKGAPQNA
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKISNLLIHLHNNIDAFTLKPRHVEQIRQALPEVAITVSASDSDFMDRLPDAEYALVWVF
CCHHHEEEEEECCCCEEEECHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEEE
KPEWYATAPRLKALFTPAAGRDWVAADPSGRVMTCHGSFHGRIMRESLLSMMLYFNRRLG
CCCHHCCCCHHHHEECCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHC
QSLEDQGRRMWGRPDYSGCVGLFSQRVMIVGLGALGQSMAELLKAFGAHVTGVKRNPESF
CCHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEECCCCCCHHC
AGSPCVDRVISFDALEEALPEADHVVLLLPGGTATDGIFTARHFNAMRPGAYLYNLGRGN
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEHHHHCCCCCCCEEEECCCCC
CYREEDLVAALEDGRLAGAGLDVFAEEPLPASSPLWEQRNVLITPHSSAISREYLDLFIQ
CCCHHHHHHHHCCCEEECCCCCEEECCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHH
EWLETLRKGAPQNA
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA