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The map label for this gene is 78221328

Identifier: 78221328

GI number: 78221328

Start: 125888

End: 127762

Strand: Reverse

Name: 78221328

Synonym: Gmet_0101

Alternate gene names: NA

Gene position: 127762-125888 (Counterclockwise)

Preceding gene: 78221329

Following gene: 78221327

Centisome position: 3.2

GC content: 64.21

Gene sequence:

>1875_bases
GTGAAGGTCGTCCTTGCCGCCATCCACCCAGTTCCCTCTCCCCAGGCCGTCCCTCTCGCCAACGCGTTCCTGAAGGCATA
CCTCGGCACCGACGAGGAGCTTGCCGCCCGCGTTGCTGTCACGCTCTGCGATTTTTTGCGTTCAACGCCAGCCGACGTCT
GTGCTGCCGAGATTCTTGCCCACGAGCCCGATGCGGTCGGCTTTTCCCTCTATCTCTGGAACACCGAGGAGTCGGGGCGG
ATTGCGGCGGAGCTTCGGCGGAACCGTCCCGGCATCACCATCTTCGCCGGCGGCCCCCAGGCAACCGCAGACCCCCTCGG
AACGCTCACCGGGGGGGGGTACGATTTTCTCATCCTCGGCGAGGGGGAGGTGCCGTTTCTCGAGGTGACGGCCCGGCTTG
CCGACGGGCGTCCTTTCCGTGGCGCGCCGGGGACGGCCTGGCTTGACGATGGAAAACTGGCAAGCCGGCCCCAGAAGCCG
GTGGCGCTCCTTGACACCATTCCGTCCCCCTTTTTGTCGGGAATCATCGAGCCCGGGCGCTACGGGGGGCTTCTCTGGCA
ACTCTCCCGCGGGTGCGACTTCGCCTGCGAGTACTGCTTCGACTACAAGGGGACCAAGGGGGTGCGGCGGTTCTCTCTGG
AGCGGGTCGACGCGGAGCTCGATTTTCTCGTCCGCCACAACGTCCCCCAGGTGTTTGTCCTCGACTCCACCTTCAACGTC
GATGCGAAGCGGGCCAAGACGATCCTGCGGATGATCCGCAAGCGCGCCCCCCATATCCACTTCCACTTCGAGGTGCGGAG
CGAGTTTCTGGACCGGGAGATGGCCCGGCTTTTTGCCGGCATTACCTGCTCTCTCCAGATCGGCCTCCAGAGTGCCGACC
CGGCCATCCTCAAGGGGATACGGCGGCATTTCGATCCGGACGACTTTCGAAAACGAGTCGCGCTTCTCAACGAAAGCGGG
GCCGTCTTCGGCTTTGATCTCATCTACGGACTCCCCGGCGACTCCCTCGCAGGCTTCAGGCAATCCCTCGACTTCGCCCT
CGCCCTCTACCCGAACCATCTGGACATCTTCCCCCTTGCCGTTCTCCCCGGCACCCCCCTTGCCGCCCGCGCGGCTCAGG
CAGGGCTCGACCACCTTCCGGCGCCCCCCTACGCGCTTACCGCCACCCCGACGTTTCCGGCCCCGGACATGAAACGTGCC
GCCCGCCTTGCCGGAGCCTGCGACATCTTCTATAGCCGGGGAAAAGCGGTCGCCTGGTTCAACGGCGTCATTGCCCCCCT
TTCCCTGACGCCGGCAGAGTTTCTCGACGCCTTCGGCGGATGGCTCGCACGCCAAGGGATTGAAGCCCGGGAGGAGGCCT
TGGGCGACGAGGCGATCTGGTCGCTGCAGCGCTCGTTTCTTGCCCACATCTTTGCCGACCGGCGGGTGCGTCGTCTTCTC
CCCGTGGCTCTCGACCTGGTCGATTATCACTATCATTACGCGGCGGCGCTTCTGGCGCCGCCGCCGGAGCTTCCCACCGA
CCGCAGCCTCGCCGGCTTGAATATTCTCGATCAGGTAGTGTGTCTTGCTCCATCCACGAGGCTTGCCCGCTTCTCCTATG
AAATCACCGATCTGCTGGAAGCGGGCAACGCAGACCTTCGGGAAATCTGTGCATGTTTCTCGCCCAGTCCGTCATGGGCC
GTCATTTATCCCCGCGGCAACGACATATTTACCGAATCTCTCCTGGAGTCCTATTTCCTTCTTCTCGAGGGGCTCGACGG
CTGCTGCCGGGCCGGTGATGTCGTTGCGCGACTCGCCCTTTCCGGAGAGGGGGCAGCGGAATTCCTCAAATTTGCCGCCG
CGGAGGGGATTGTCACGGTACGGAACTGCCCCTGA

Upstream 100 bases:

>100_bases
CGGCGTCCCCAAGATCAAGGCCAGGCCCCGTCGGTCATCCTAACCATCACCGTCATGCCATTCCTGGCGGAGTGGTCTCA
TTTTACCCCCGGAGGAGTCC

Downstream 100 bases:

>100_bases
CGCAGCAGGGATAATGTTATGTCGTGTTTAAGCCGCTGCGATTGCCTCTATGTATAAAAAAAGTATACTATTTTGTTGCA
TTTTCCCCCCTTGGGTATTA

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MKVVLAAIHPVPSPQAVPLANAFLKAYLGTDEELAARVAVTLCDFLRSTPADVCAAEILAHEPDAVGFSLYLWNTEESGR
IAAELRRNRPGITIFAGGPQATADPLGTLTGGGYDFLILGEGEVPFLEVTARLADGRPFRGAPGTAWLDDGKLASRPQKP
VALLDTIPSPFLSGIIEPGRYGGLLWQLSRGCDFACEYCFDYKGTKGVRRFSLERVDAELDFLVRHNVPQVFVLDSTFNV
DAKRAKTILRMIRKRAPHIHFHFEVRSEFLDREMARLFAGITCSLQIGLQSADPAILKGIRRHFDPDDFRKRVALLNESG
AVFGFDLIYGLPGDSLAGFRQSLDFALALYPNHLDIFPLAVLPGTPLAARAAQAGLDHLPAPPYALTATPTFPAPDMKRA
ARLAGACDIFYSRGKAVAWFNGVIAPLSLTPAEFLDAFGGWLARQGIEAREEALGDEAIWSLQRSFLAHIFADRRVRRLL
PVALDLVDYHYHYAAALLAPPPELPTDRSLAGLNILDQVVCLAPSTRLARFSYEITDLLEAGNADLREICACFSPSPSWA
VIYPRGNDIFTESLLESYFLLLEGLDGCCRAGDVVARLALSGEGAAEFLKFAAAEGIVTVRNCP

Sequences:

>Translated_624_residues
MKVVLAAIHPVPSPQAVPLANAFLKAYLGTDEELAARVAVTLCDFLRSTPADVCAAEILAHEPDAVGFSLYLWNTEESGR
IAAELRRNRPGITIFAGGPQATADPLGTLTGGGYDFLILGEGEVPFLEVTARLADGRPFRGAPGTAWLDDGKLASRPQKP
VALLDTIPSPFLSGIIEPGRYGGLLWQLSRGCDFACEYCFDYKGTKGVRRFSLERVDAELDFLVRHNVPQVFVLDSTFNV
DAKRAKTILRMIRKRAPHIHFHFEVRSEFLDREMARLFAGITCSLQIGLQSADPAILKGIRRHFDPDDFRKRVALLNESG
AVFGFDLIYGLPGDSLAGFRQSLDFALALYPNHLDIFPLAVLPGTPLAARAAQAGLDHLPAPPYALTATPTFPAPDMKRA
ARLAGACDIFYSRGKAVAWFNGVIAPLSLTPAEFLDAFGGWLARQGIEAREEALGDEAIWSLQRSFLAHIFADRRVRRLL
PVALDLVDYHYHYAAALLAPPPELPTDRSLAGLNILDQVVCLAPSTRLARFSYEITDLLEAGNADLREICACFSPSPSWA
VIYPRGNDIFTESLLESYFLLLEGLDGCCRAGDVVARLALSGEGAAEFLKFAAAEGIVTVRNCP
>Mature_624_residues
MKVVLAAIHPVPSPQAVPLANAFLKAYLGTDEELAARVAVTLCDFLRSTPADVCAAEILAHEPDAVGFSLYLWNTEESGR
IAAELRRNRPGITIFAGGPQATADPLGTLTGGGYDFLILGEGEVPFLEVTARLADGRPFRGAPGTAWLDDGKLASRPQKP
VALLDTIPSPFLSGIIEPGRYGGLLWQLSRGCDFACEYCFDYKGTKGVRRFSLERVDAELDFLVRHNVPQVFVLDSTFNV
DAKRAKTILRMIRKRAPHIHFHFEVRSEFLDREMARLFAGITCSLQIGLQSADPAILKGIRRHFDPDDFRKRVALLNESG
AVFGFDLIYGLPGDSLAGFRQSLDFALALYPNHLDIFPLAVLPGTPLAARAAQAGLDHLPAPPYALTATPTFPAPDMKRA
ARLAGACDIFYSRGKAVAWFNGVIAPLSLTPAEFLDAFGGWLARQGIEAREEALGDEAIWSLQRSFLAHIFADRRVRRLL
PVALDLVDYHYHYAAALLAPPPELPTDRSLAGLNILDQVVCLAPSTRLARFSYEITDLLEAGNADLREICACFSPSPSWA
VIYPRGNDIFTESLLESYFLLLEGLDGCCRAGDVVARLALSGEGAAEFLKFAAAEGIVTVRNCP

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 67967; Mature: 67967

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVLAAIHPVPSPQAVPLANAFLKAYLGTDEELAARVAVTLCDFLRSTPADVCAAEILA
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HEPDAVGFSLYLWNTEESGRIAAELRRNRPGITIFAGGPQATADPLGTLTGGGYDFLILG
CCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEC
EGEVPFLEVTARLADGRPFRGAPGTAWLDDGKLASRPQKPVALLDTIPSPFLSGIIEPGR
CCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCC
YGGLLWQLSRGCDFACEYCFDYKGTKGVRRFSLERVDAELDFLVRHNVPQVFVLDSTFNV
CCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHCCCCEEEEECCCCCC
DAKRAKTILRMIRKRAPHIHFHFEVRSEFLDREMARLFAGITCSLQIGLQSADPAILKGI
CHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
RRHFDPDDFRKRVALLNESGAVFGFDLIYGLPGDSLAGFRQSLDFALALYPNHLDIFPLA
HHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCEEEEECCCCCCEEEEE
VLPGTPLAARAAQAGLDHLPAPPYALTATPTFPAPDMKRAARLAGACDIFYSRGKAVAWF
ECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
NGVIAPLSLTPAEFLDAFGGWLARQGIEAREEALGDEAIWSLQRSFLAHIFADRRVRRLL
CCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
PVALDLVDYHYHYAAALLAPPPELPTDRSLAGLNILDQVVCLAPSTRLARFSYEITDLLE
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGNADLREICACFSPSPSWAVIYPRGNDIFTESLLESYFLLLEGLDGCCRAGDVVARLAL
CCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGEGAAEFLKFAAAEGIVTVRNCP
CCCCHHHHHHHHHHCCEEEECCCC
>Mature Secondary Structure
MKVVLAAIHPVPSPQAVPLANAFLKAYLGTDEELAARVAVTLCDFLRSTPADVCAAEILA
CEEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
HEPDAVGFSLYLWNTEESGRIAAELRRNRPGITIFAGGPQATADPLGTLTGGGYDFLILG
CCCCCCEEEEEEECCCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEC
EGEVPFLEVTARLADGRPFRGAPGTAWLDDGKLASRPQKPVALLDTIPSPFLSGIIEPGR
CCCCCHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHCCCCHHHHHCCCCCC
YGGLLWQLSRGCDFACEYCFDYKGTKGVRRFSLERVDAELDFLVRHNVPQVFVLDSTFNV
CCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHCCCCEEEEECCCCCC
DAKRAKTILRMIRKRAPHIHFHFEVRSEFLDREMARLFAGITCSLQIGLQSADPAILKGI
CHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHH
RRHFDPDDFRKRVALLNESGAVFGFDLIYGLPGDSLAGFRQSLDFALALYPNHLDIFPLA
HHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCEEEEECCCCCCEEEEE
VLPGTPLAARAAQAGLDHLPAPPYALTATPTFPAPDMKRAARLAGACDIFYSRGKAVAWF
ECCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
NGVIAPLSLTPAEFLDAFGGWLARQGIEAREEALGDEAIWSLQRSFLAHIFADRRVRRLL
CCEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
PVALDLVDYHYHYAAALLAPPPELPTDRSLAGLNILDQVVCLAPSTRLARFSYEITDLLE
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGNADLREICACFSPSPSWAVIYPRGNDIFTESLLESYFLLLEGLDGCCRAGDVVARLAL
CCCCCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SGEGAAEFLKFAAAEGIVTVRNCP
CCCCHHHHHHHHHHCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]