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The map label for this gene is nifA [H]

Identifier: 78221315

GI number: 78221315

Start: 110601

End: 112076

Strand: Direct

Name: nifA [H]

Synonym: Gmet_0088

Alternate gene names: 78221315

Gene position: 110601-112076 (Clockwise)

Preceding gene: 78221308

Following gene: 78221317

Centisome position: 2.77

GC content: 68.22

Gene sequence:

>1476_bases
ATGAAAGCTATCGAGACAATACTGGCGACGGAGCGGGATTTTTTTGCCAAGGTGGCCCGGGCCGCCTTCCTCAATCCCTT
TGGCGAGAGCCGCGACGAACTGGACCTGCTCCTGGCGGGAACTGGTGCCGGTGACCGGGGCGGAGTGCTTGCCGCCATCA
TTGCCCGGGTGGCCGAACGGACGGCCGTCCTGCAGCGGGAACTGCGGGGCAACCACCACCGCCTGCCGGCTGCCGACCGG
GAAACGGTCCGGACTGTGCTCCTCTTCGATCTCTTTCATCGGTTCCTGGACTCCTTCGACCGCCTGATCCGGGAGCAGGC
CGTCGGCGGCGACTCCCCCTGCCCCGTCCCCTTTGCCGGCGGCTTCCAGGCCGCCTTCGCGGAGGCGGGCTTCTCCGAGA
CGGAAAGCCGCCGCTACCTGGGCCTCTTCTACCAGCTCCGGCGGGCCTTCTACTTCATCCGGGAAGGGCTCGCCGGTTCC
AGCCCGTCGCTGCGCCGGGTGCGGGAACACCTCTGGCAGACCCTCTTCACCCACAACATCCGGTGGTTCGACCAGTACCT
CTGGGACCGGATGGAGGACTTCTCCGTACTCATCCTCGGGGAGACCGGCACCGGCAAGGGGGCCGCAGCCGCGGCCATCG
GCCGGTCGGGCTTCATCCCCTTCGACCCGAAAACCGGCCGGTTCAGCGAAAGCTTCACCCGCAACTTCCTGGCGGTGAAC
CTCTCCCAGTACCCGGAGGGGGTCCTGGAGTCGGAGCTCTTCGGCCACCGGAAGGGGGCCTTCACCGGCGCCATCGATCA
CCATGAGGGGGTCTTCAGCCGCTGCACCCCCCACGGCGCCATCTTCCTGGACGAGATCGGCGAGGTGCCGCTGCCGGTGC
AGGTGAAGCTCCTCCAGGTGCTCCAGGAGCGGAGCTTCTCGCCGGTGGGGAGCCACCAGCGGCTCCGCTTCAGCGGCCGG
GTCATCGCCGCCACCAACCGGGACCTGACCCGCCTGCGGGAGCAGGGGGAGTTCCGGGACGACTTCTACTACCGCCTCTG
TTCCGACGTCATCACCATCCCACCCCTGCGGCAGCGGCTCCAGGAATGCCCCGACGAGCTGGGGGAGCTGGTGGCGGGAA
TCCTCACGCGCCTGACCGGGACCGCGCCGAAGCGGGAGGTGCAGCTATTGCGCAAGGCCATCGAACGGGAAACGGGAAAG
GGATACCACTGGCCGGGAAACGTGCGGGAGCTGGAGCAGGCGGTGCGGCGGGTACTGGTGACGGGACATTACCGGGGGGA
AGAGAAAGGGGCGCCGTCCGGCGTGACGGAGCAGTTCCTGGCGGACGTGGCGGCGGGGGGGCTCGATGCCCAGGGGCTTC
TGGCCGGCTACTGCCGCATCCTGTACGAGCGGCACGACACCTACGAGGAGGTGGCCCGCCGGGCCAACCTGGACCGGCGG
ACGGTGAAGAAGTATCTGCAGCAAGCAGGGGAGTGA

Upstream 100 bases:

>100_bases
CCAATGATTCTGGATTGTTGCAGTCAGACGGCAATCGCTCCTGCTGATTAGCGGCAAATCGTGATTGACATTTTATGAAT
GGCAACTATACATCGAATGT

Downstream 100 bases:

>100_bases
TATCAAACAAAAAAATCAAATCGCGGATGGATTTAGGTCAGATTTGGTCGGGCCGAATGAGTACAACCGCAGGCGTAGCA
GCGCTACGTCGAGGATTGTG

Product: sigma-54 factor, interaction region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKAIETILATERDFFAKVARAAFLNPFGESRDELDLLLAGTGAGDRGGVLAAIIARVAERTAVLQRELRGNHHRLPAADR
ETVRTVLLFDLFHRFLDSFDRLIREQAVGGDSPCPVPFAGGFQAAFAEAGFSETESRRYLGLFYQLRRAFYFIREGLAGS
SPSLRRVREHLWQTLFTHNIRWFDQYLWDRMEDFSVLILGETGTGKGAAAAAIGRSGFIPFDPKTGRFSESFTRNFLAVN
LSQYPEGVLESELFGHRKGAFTGAIDHHEGVFSRCTPHGAIFLDEIGEVPLPVQVKLLQVLQERSFSPVGSHQRLRFSGR
VIAATNRDLTRLREQGEFRDDFYYRLCSDVITIPPLRQRLQECPDELGELVAGILTRLTGTAPKREVQLLRKAIERETGK
GYHWPGNVRELEQAVRRVLVTGHYRGEEKGAPSGVTEQFLADVAAGGLDAQGLLAGYCRILYERHDTYEEVARRANLDRR
TVKKYLQQAGE

Sequences:

>Translated_491_residues
MKAIETILATERDFFAKVARAAFLNPFGESRDELDLLLAGTGAGDRGGVLAAIIARVAERTAVLQRELRGNHHRLPAADR
ETVRTVLLFDLFHRFLDSFDRLIREQAVGGDSPCPVPFAGGFQAAFAEAGFSETESRRYLGLFYQLRRAFYFIREGLAGS
SPSLRRVREHLWQTLFTHNIRWFDQYLWDRMEDFSVLILGETGTGKGAAAAAIGRSGFIPFDPKTGRFSESFTRNFLAVN
LSQYPEGVLESELFGHRKGAFTGAIDHHEGVFSRCTPHGAIFLDEIGEVPLPVQVKLLQVLQERSFSPVGSHQRLRFSGR
VIAATNRDLTRLREQGEFRDDFYYRLCSDVITIPPLRQRLQECPDELGELVAGILTRLTGTAPKREVQLLRKAIERETGK
GYHWPGNVRELEQAVRRVLVTGHYRGEEKGAPSGVTEQFLADVAAGGLDAQGLLAGYCRILYERHDTYEEVARRANLDRR
TVKKYLQQAGE
>Mature_491_residues
MKAIETILATERDFFAKVARAAFLNPFGESRDELDLLLAGTGAGDRGGVLAAIIARVAERTAVLQRELRGNHHRLPAADR
ETVRTVLLFDLFHRFLDSFDRLIREQAVGGDSPCPVPFAGGFQAAFAEAGFSETESRRYLGLFYQLRRAFYFIREGLAGS
SPSLRRVREHLWQTLFTHNIRWFDQYLWDRMEDFSVLILGETGTGKGAAAAAIGRSGFIPFDPKTGRFSESFTRNFLAVN
LSQYPEGVLESELFGHRKGAFTGAIDHHEGVFSRCTPHGAIFLDEIGEVPLPVQVKLLQVLQERSFSPVGSHQRLRFSGR
VIAATNRDLTRLREQGEFRDDFYYRLCSDVITIPPLRQRLQECPDELGELVAGILTRLTGTAPKREVQLLRKAIERETGK
GYHWPGNVRELEQAVRRVLVTGHYRGEEKGAPSGVTEQFLADVAAGGLDAQGLLAGYCRILYERHDTYEEVARRANLDRR
TVKKYLQQAGE

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=244, Percent_Identity=39.7540983606557, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788550, Length=228, Percent_Identity=40.3508771929825, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI87082117, Length=230, Percent_Identity=41.7391304347826, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=41.7391304347826, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1790437, Length=241, Percent_Identity=41.0788381742739, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=33.6477987421384, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786524, Length=269, Percent_Identity=36.8029739776952, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=40.948275862069, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1790299, Length=229, Percent_Identity=37.9912663755458, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=36.7965367965368, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=34.3612334801762, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1789828, Length=267, Percent_Identity=31.8352059925094, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87081858, Length=256, Percent_Identity=30.078125, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55202; Mature: 55202

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIETILATERDFFAKVARAAFLNPFGESRDELDLLLAGTGAGDRGGVLAAIIARVAER
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
TAVLQRELRGNHHRLPAADRETVRTVLLFDLFHRFLDSFDRLIREQAVGGDSPCPVPFAG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFQAAFAEAGFSETESRRYLGLFYQLRRAFYFIREGLAGSSPSLRRVREHLWQTLFTHNI
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RWFDQYLWDRMEDFSVLILGETGTGKGAAAAAIGRSGFIPFDPKTGRFSESFTRNFLAVN
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEEE
LSQYPEGVLESELFGHRKGAFTGAIDHHEGVFSRCTPHGAIFLDEIGEVPLPVQVKLLQV
HHHCCCHHHHHHHHHCCCCCCCCCCCHHCCHHHHCCCCCCEEHHHHCCCCCCHHHHHHHH
LQERSFSPVGSHQRLRFSGRVIAATNRDLTRLREQGEFRDDFYYRLCSDVITIPPLRQRL
HHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHH
QECPDELGELVAGILTRLTGTAPKREVQLLRKAIERETGKGYHWPGNVRELEQAVRRVLV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TGHYRGEEKGAPSGVTEQFLADVAAGGLDAQGLLAGYCRILYERHDTYEEVARRANLDRR
HCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHH
TVKKYLQQAGE
HHHHHHHHCCC
>Mature Secondary Structure
MKAIETILATERDFFAKVARAAFLNPFGESRDELDLLLAGTGAGDRGGVLAAIIARVAER
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
TAVLQRELRGNHHRLPAADRETVRTVLLFDLFHRFLDSFDRLIREQAVGGDSPCPVPFAG
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GFQAAFAEAGFSETESRRYLGLFYQLRRAFYFIREGLAGSSPSLRRVREHLWQTLFTHNI
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RWFDQYLWDRMEDFSVLILGETGTGKGAAAAAIGRSGFIPFDPKTGRFSESFTRNFLAVN
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEEE
LSQYPEGVLESELFGHRKGAFTGAIDHHEGVFSRCTPHGAIFLDEIGEVPLPVQVKLLQV
HHHCCCHHHHHHHHHCCCCCCCCCCCHHCCHHHHCCCCCCEEHHHHCCCCCCHHHHHHHH
LQERSFSPVGSHQRLRFSGRVIAATNRDLTRLREQGEFRDDFYYRLCSDVITIPPLRQRL
HHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHH
QECPDELGELVAGILTRLTGTAPKREVQLLRKAIERETGKGYHWPGNVRELEQAVRRVLV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TGHYRGEEKGAPSGVTEQFLADVAAGGLDAQGLLAGYCRILYERHDTYEEVARRANLDRR
HCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHH
TVKKYLQQAGE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]