Click here to switch to the map view.

The map label for this gene is rpfC [H]

Identifier: 78221304

GI number: 78221304

Start: 93589

End: 95574

Strand: Reverse

Name: rpfC [H]

Synonym: Gmet_0077

Alternate gene names: 78221304

Gene position: 95574-93589 (Counterclockwise)

Preceding gene: 78221305

Following gene: 78221303

Centisome position: 2.39

GC content: 63.09

Gene sequence:

>1986_bases
ATGAATCTCGAAACCTGGCGGATACGCAACTGGCGACTCTTCACGAAAATATTCCTCATGTCGACGGTGGCGGTGTTCGT
CATCATAGCGGGGCTGCTGGGGTTCTTCCTGCCGCTTCTGGAATCGAAGCTGATCCACGAGCGGACGGATAACGCGAGCC
ATATCGTGGACGCGGCACGCTCCATAGCCGCCCACTACCATTCCCTCCAACTCCGGGGGGAGCTCTCCGAGCCAGAGGCC
CGCAGACTGGCCGTGAATACCCTTCGTGCATTGCGCTACAATGTCAACGGCTACGTCTGGGTCCATGACCTGGAGAACAG
GATGGTGATGCACCCCCTGCAACCCGAATTGGAGGGGCACTATCTCACCCGCTTGGCCGACGCCCGGGGGAAGCCCCTCT
TCCTGACCATGAACAACCTTGTGCGCCGGGAGGGGAAGGGAGAAGTCGACTACCTCTGGCCCCGCCCGGGCGAACAAGAG
CCGAGCCGCAAAGTGTCCTATGTTTCCCTCTTTGAGCCGTGGCAGTGGGTTCTGGGGAGCGGCGTATACCTGGACGACGT
GCAGGACGAAATTTCCATGCTGGAGGCGCGAGTCATAACGGGGGCACTGGTGACGCTGCTTCTCTCCGTGGTCTTTTCCG
CCTATGCCGCCTACCGCATCAACCGCCCCCTCCGGCAGGCGCTCTTCTGTGCGGCCGCCGCCATGGGGCACGACACGTCG
GTGGTGGAGGACAAAAGCCATGACGAGACACACCAGGTGCTCCATACCATCGAGCGGATGGTCAACGACCTGAAGCGTTC
CCGAGACGAAGCGGAGTCCGCCAGCAGGATGAAGGGGGAGTTCCTGGCCAACATGAGCCACGAGATCCGGACCCCCATGA
ACGCCATCATCGGCATGACCGAACTGACCCTGGAAACGCCCCTTTCACCCTCCCAGCGCGAAAACCTGGAGATCATCGGT
TCATCGGCCGAGAGCCTGCTGGGGCTGCTGAACGATATCCTGGATTTCTCGAGGATCGAGGCCGGGCGCCTCTTGCTGGA
GAAGGTCCCCTTCGACCTCCGGAACACCGTGGAGGGAGCCCTGGACGTGGTAGCCCCCCAGGCCCACGGCAAGGGGCTGG
AGCTGATCCTCGACGTGGCACGGGACCTCCCGGAGGCATTCATCGGCGATCCAACCCGCCTGCGGCAGGTGCTGATCAAC
CTCCTCAGCAATGCCGTCAAGTTCACCGCCAAGGGAGAAGTGGGCCTGAAGGTGGAGCGCGCCCCCTCTTCCGGCACGGA
CGACGGCGTGATGCTGCTCTTCACGGTTTCGGACACCGGCATCGGCATCCCCGCCGACAAGCTCTCCCTCATATTCGAGT
CGTTCACCCAGGCCGACGGCTCCACCACCAGGAAATACGGGGGGTCGGGGCTGGGGCTCGCCATTTGCCGCGCCCTGGTG
TCGATGATGGGAGGGGATATCTCCGTAAAGAGCGCCGTCGGAGTTGGGAGCACCTTCCGGTTCACGGCCCGGTTGGGGAC
CTGCGCTCTCCAGAACCGGGAAATCAGGCAGGCAGGGATACCCCGGTTTGGCCGGGAGGTGAAGATCCTTCTCGCCGAGG
ATAATCCCATGAATCTCAAGGTTTCCCGGCTCCTCCTGGAGATGATGGGGTGCCAGGTTGTTGCGGTGGAGAACGGCGGC
GATGTCGTGAGCGCCATGAAGGGGGAGCGATTCGACGTGGTGCTGATGGATATCCAGATGCCGGTGCTGGACGGCCTGGA
GGCGACCCGCGTGATACGGAGCGGCTCAGAGAGGGGCATCGACCAGACCACGCCAGTCATCGCCATGACCGCCCACGCCC
TCAAGGGGTACGAGAAAGTCTGCCACGAGGCGGGGATGAACGATTTCATCTCCAAGCCGGTCCGGGCAGGGGAGCTGTAC
AACGTCATCCTCCGCAATCTCCCCCCGGCCCCCGGCGGTGGCGGGGTCACCCCTTCCGCGCTCTGA

Upstream 100 bases:

>100_bases
TTTTTTGCCGAAAAGAGAGTAATGATGCGCGGTGCCGGAACCCGGCACGACTCCCACCTTTGATGCCTTCCATCGTTCGG
CAGAAGGACTGCTGCGGGCC

Downstream 100 bases:

>100_bases
ATTTCCCGACCAGCCCACAGCCACAAGAGAGAAGCGAACGCATCCCGGCCGTTTGAACGATGAGCCGCGGCATTCATGGC
CGCGTCGCCTTGTCATTTTC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MNLETWRIRNWRLFTKIFLMSTVAVFVIIAGLLGFFLPLLESKLIHERTDNASHIVDAARSIAAHYHSLQLRGELSEPEA
RRLAVNTLRALRYNVNGYVWVHDLENRMVMHPLQPELEGHYLTRLADARGKPLFLTMNNLVRREGKGEVDYLWPRPGEQE
PSRKVSYVSLFEPWQWVLGSGVYLDDVQDEISMLEARVITGALVTLLLSVVFSAYAAYRINRPLRQALFCAAAAMGHDTS
VVEDKSHDETHQVLHTIERMVNDLKRSRDEAESASRMKGEFLANMSHEIRTPMNAIIGMTELTLETPLSPSQRENLEIIG
SSAESLLGLLNDILDFSRIEAGRLLLEKVPFDLRNTVEGALDVVAPQAHGKGLELILDVARDLPEAFIGDPTRLRQVLIN
LLSNAVKFTAKGEVGLKVERAPSSGTDDGVMLLFTVSDTGIGIPADKLSLIFESFTQADGSTTRKYGGSGLGLAICRALV
SMMGGDISVKSAVGVGSTFRFTARLGTCALQNREIRQAGIPRFGREVKILLAEDNPMNLKVSRLLLEMMGCQVVAVENGG
DVVSAMKGERFDVVLMDIQMPVLDGLEATRVIRSGSERGIDQTTPVIAMTAHALKGYEKVCHEAGMNDFISKPVRAGELY
NVILRNLPPAPGGGGVTPSAL

Sequences:

>Translated_661_residues
MNLETWRIRNWRLFTKIFLMSTVAVFVIIAGLLGFFLPLLESKLIHERTDNASHIVDAARSIAAHYHSLQLRGELSEPEA
RRLAVNTLRALRYNVNGYVWVHDLENRMVMHPLQPELEGHYLTRLADARGKPLFLTMNNLVRREGKGEVDYLWPRPGEQE
PSRKVSYVSLFEPWQWVLGSGVYLDDVQDEISMLEARVITGALVTLLLSVVFSAYAAYRINRPLRQALFCAAAAMGHDTS
VVEDKSHDETHQVLHTIERMVNDLKRSRDEAESASRMKGEFLANMSHEIRTPMNAIIGMTELTLETPLSPSQRENLEIIG
SSAESLLGLLNDILDFSRIEAGRLLLEKVPFDLRNTVEGALDVVAPQAHGKGLELILDVARDLPEAFIGDPTRLRQVLIN
LLSNAVKFTAKGEVGLKVERAPSSGTDDGVMLLFTVSDTGIGIPADKLSLIFESFTQADGSTTRKYGGSGLGLAICRALV
SMMGGDISVKSAVGVGSTFRFTARLGTCALQNREIRQAGIPRFGREVKILLAEDNPMNLKVSRLLLEMMGCQVVAVENGG
DVVSAMKGERFDVVLMDIQMPVLDGLEATRVIRSGSERGIDQTTPVIAMTAHALKGYEKVCHEAGMNDFISKPVRAGELY
NVILRNLPPAPGGGGVTPSAL
>Mature_661_residues
MNLETWRIRNWRLFTKIFLMSTVAVFVIIAGLLGFFLPLLESKLIHERTDNASHIVDAARSIAAHYHSLQLRGELSEPEA
RRLAVNTLRALRYNVNGYVWVHDLENRMVMHPLQPELEGHYLTRLADARGKPLFLTMNNLVRREGKGEVDYLWPRPGEQE
PSRKVSYVSLFEPWQWVLGSGVYLDDVQDEISMLEARVITGALVTLLLSVVFSAYAAYRINRPLRQALFCAAAAMGHDTS
VVEDKSHDETHQVLHTIERMVNDLKRSRDEAESASRMKGEFLANMSHEIRTPMNAIIGMTELTLETPLSPSQRENLEIIG
SSAESLLGLLNDILDFSRIEAGRLLLEKVPFDLRNTVEGALDVVAPQAHGKGLELILDVARDLPEAFIGDPTRLRQVLIN
LLSNAVKFTAKGEVGLKVERAPSSGTDDGVMLLFTVSDTGIGIPADKLSLIFESFTQADGSTTRKYGGSGLGLAICRALV
SMMGGDISVKSAVGVGSTFRFTARLGTCALQNREIRQAGIPRFGREVKILLAEDNPMNLKVSRLLLEMMGCQVVAVENGG
DVVSAMKGERFDVVLMDIQMPVLDGLEATRVIRSGSERGIDQTTPVIAMTAHALKGYEKVCHEAGMNDFISKPVRAGELY
NVILRNLPPAPGGGGVTPSAL

Specific function: Involved in the positive regulation of synthesis of extracellular enzymes and polysaccharide, and hence in pathogenicity. Can restore function to the non-pathogenic mutant 8237 [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=262, Percent_Identity=46.5648854961832, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI48994928, Length=389, Percent_Identity=37.0179948586118, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI87081816, Length=342, Percent_Identity=38.3040935672515, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1788713, Length=379, Percent_Identity=31.3984168865435, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=42.741935483871, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786600, Length=258, Percent_Identity=31.7829457364341, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788393, Length=256, Percent_Identity=30.078125, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=32.4675324675325, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1786783, Length=312, Percent_Identity=29.4871794871795, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=27.7310924369748, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=246, Percent_Identity=27.6422764227642, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322044, Length=123, Percent_Identity=37.3983739837398, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322000, Length=119, Percent_Identity=36.1344537815126, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 72760; Mature: 72760

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLETWRIRNWRLFTKIFLMSTVAVFVIIAGLLGFFLPLLESKLIHERTDNASHIVDAAR
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SIAAHYHSLQLRGELSEPEARRLAVNTLRALRYNVNGYVWVHDLENRMVMHPLQPELEGH
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCH
YLTRLADARGKPLFLTMNNLVRREGKGEVDYLWPRPGEQEPSRKVSYVSLFEPWQWVLGS
HHHHHHHCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEHHCHHHHHHCC
GVYLDDVQDEISMLEARVITGALVTLLLSVVFSAYAAYRINRPLRQALFCAAAAMGHDTS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
VVEDKSHDETHQVLHTIERMVNDLKRSRDEAESASRMKGEFLANMSHEIRTPMNAIIGMT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHCHH
ELTLETPLSPSQRENLEIIGSSAESLLGLLNDILDFSRIEAGRLLLEKVPFDLRNTVEGA
HHEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LDVVAPQAHGKGLELILDVARDLPEAFIGDPTRLRQVLINLLSNAVKFTAKGEVGLKVER
HHHHCCCCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
APSSGTDDGVMLLFTVSDTGIGIPADKLSLIFESFTQADGSTTRKYGGSGLGLAICRALV
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHH
SMMGGDISVKSAVGVGSTFRFTARLGTCALQNREIRQAGIPRFGREVKILLAEDNPMNLK
HHHCCCEEEEHHCCCCCCEEEHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCCCHH
VSRLLLEMMGCQVVAVENGGDVVSAMKGERFDVVLMDIQMPVLDGLEATRVIRSGSERGI
HHHHHHHHHCCEEEEECCCCCCHHHCCCCCEEEEEEEECCHHHCCHHHHHHHHCCCCCCC
DQTTPVIAMTAHALKGYEKVCHEAGMNDFISKPVRAGELYNVILRNLPPAPGGGGVTPSA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
L
C
>Mature Secondary Structure
MNLETWRIRNWRLFTKIFLMSTVAVFVIIAGLLGFFLPLLESKLIHERTDNASHIVDAAR
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SIAAHYHSLQLRGELSEPEARRLAVNTLRALRYNVNGYVWVHDLENRMVMHPLQPELEGH
HHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCH
YLTRLADARGKPLFLTMNNLVRREGKGEVDYLWPRPGEQEPSRKVSYVSLFEPWQWVLGS
HHHHHHHCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEHHCHHHHHHCC
GVYLDDVQDEISMLEARVITGALVTLLLSVVFSAYAAYRINRPLRQALFCAAAAMGHDTS
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
VVEDKSHDETHQVLHTIERMVNDLKRSRDEAESASRMKGEFLANMSHEIRTPMNAIIGMT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCHHHHHHCHH
ELTLETPLSPSQRENLEIIGSSAESLLGLLNDILDFSRIEAGRLLLEKVPFDLRNTVEGA
HHEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LDVVAPQAHGKGLELILDVARDLPEAFIGDPTRLRQVLINLLSNAVKFTAKGEVGLKVER
HHHHCCCCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
APSSGTDDGVMLLFTVSDTGIGIPADKLSLIFESFTQADGSTTRKYGGSGLGLAICRALV
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHH
SMMGGDISVKSAVGVGSTFRFTARLGTCALQNREIRQAGIPRFGREVKILLAEDNPMNLK
HHHCCCEEEEHHCCCCCCEEEHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCCCHH
VSRLLLEMMGCQVVAVENGGDVVSAMKGERFDVVLMDIQMPVLDGLEATRVIRSGSERGI
HHHHHHHHHCCEEEEECCCCCCHHHCCCCCEEEEEEEECCHHHCCHHHHHHHHCCCCCCC
DQTTPVIAMTAHALKGYEKVCHEAGMNDFISKPVRAGELYNVILRNLPPAPGGGGVTPSA
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1645442 [H]