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The map label for this gene is ywiE [H]

Identifier: 78221292

GI number: 78221292

Start: 79368

End: 80801

Strand: Reverse

Name: ywiE [H]

Synonym: Gmet_0065

Alternate gene names: 78221292

Gene position: 80801-79368 (Counterclockwise)

Preceding gene: 78221297

Following gene: 78221289

Centisome position: 2.02

GC content: 64.5

Gene sequence:

>1434_bases
GTGATCGACGAACTGGCCTTAGCACTGGGAACAGCGGCCCTCATTGCCCTCTCGCTCTTTTCGGCAGGGCACGCCTTGAT
CATGAAGCGCGATCCCCGTTCCTCCCTGGGGTGGATCGTCACCTGCCTCACCATCCCCCTGCTCGGCCCCTTTTTTTACT
GGGGGATGGGGGTCAACCGGATCTTCCGCCGCGCCCGTACATGGCTCGAAAGTGGCCGTCGCCTGGCCGACCCCGAGTTC
TTCAAATCGACCCTCGAACAGAACACCGCCACGGCCCTCCCCCCCGGCGCCGAGTACCTGAGCGAGCTCCGCCGTCTGGC
CGACCGGGTGGTGAGCACTCCCCTCGTGACGGGAAATCGGTTCACCCCCCTGGTAAACGGCGAAGGTGCCTATCCTGCCA
TGCTCGAAGCCATCGCCGGCGCCCGCCGGTCGATCCACCTCTCCACCTACATCTTCGACTCGGACGCCGCGGGTCGCCGC
TTCATCGATGCCCTGGCCGACGCCGCGAGGCGGGGGGTCGACGTGCGGGTCATCATCGACGGCCTCGGCGAAAAGTATTC
CTTCCCCCGTGCCCGAACGCTCATGGAGAGGACCGGCATCCGGGTCGCCCGCTTCCTGCCGTTGAGGCAGGGGTTTTACA
TGAATCTGCGCAACCACAGGAAAATGCTGATTATAGACGGGGAACGGGCGTTCACGGGAGGGATGAACATCGGTGACCGC
CACATGGTGGAGCGGCCGCTCCCGACGGTGGTGAAGGATCTGCACTTTGCCGTCGCAGGGCCGGTGGTGGGCGAGCTTCA
ACAGATCTTCCTGGAAGACTGGTACTTCGTTTCCGGAGAGCTCCTGGACAACCGGCACCACTTCCCCCCCCTCCCCGCGG
CGGGGCACGCCATATCCCGGGCCATCGGCGACGGCCCCGACAAGGAGTTCCGCAAGCTCTTCCTGATCCTCATGGGGGCA
CTCTCCTGCGCCACCCGGCAGGTGCAGATCATGACCCCCTATTTCATTCCCGACCGGGCACTGGTGGCAGCCCTTGTCAC
CACTGCCCTGCGTGGCGTGGAAGTGACTCTCGTCCTCCCCGAGAAGAACAACCTTCCCTTTGTCCACTGGGCCTCCCGGG
CCTACCTCTGGGAGCTCCTCCAGCAGGGGATCCGGGTCTTCTACCAACCTCCCCCCTTCGTCCACACCAAGCTCTTCATT
GTGGACGGCCTCTGGAGCCTCATCGGCTCCGCCAATCTCGATCCCCGCAGCCTCCGCCTCAACTTCGAGCTGAACCTGGA
GGTCTATGACCGGGAATTCGCCGGAACCCTGACCAAGCATGTAGCCGGCGTGCTGGCCCGGTCCCGCGAGGTAACCCTCG
AGCAAATGGACAACCGCCCCCTGCCGGAAAAGCTGCGGGACGGGGTGGCCAAGCTCTTCTCCCCCTATCTGTAG

Upstream 100 bases:

>100_bases
GCTTCGGCCTGCTGCTTGACTTGACCGGACGGTGAGCTAACCTTGACGTGTGCCGGCCGACCCGCCGCGGTTTTTGTGCA
TCTTGTTATGGAGAGGTGAT

Downstream 100 bases:

>100_bases
CCCTCCACAGAAATCCATGAAAAAGCCCGCTTGCGCGGGCCATGGTCACGTGCGGTATTTCTCGATGGCGACCAGCGCCC
GGTCGAGGGCGTCGCGGCAA

Product: cardiolipin synthetase 2

Products: NA

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MIDELALALGTAALIALSLFSAGHALIMKRDPRSSLGWIVTCLTIPLLGPFFYWGMGVNRIFRRARTWLESGRRLADPEF
FKSTLEQNTATALPPGAEYLSELRRLADRVVSTPLVTGNRFTPLVNGEGAYPAMLEAIAGARRSIHLSTYIFDSDAAGRR
FIDALADAARRGVDVRVIIDGLGEKYSFPRARTLMERTGIRVARFLPLRQGFYMNLRNHRKMLIIDGERAFTGGMNIGDR
HMVERPLPTVVKDLHFAVAGPVVGELQQIFLEDWYFVSGELLDNRHHFPPLPAAGHAISRAIGDGPDKEFRKLFLILMGA
LSCATRQVQIMTPYFIPDRALVAALVTTALRGVEVTLVLPEKNNLPFVHWASRAYLWELLQQGIRVFYQPPPFVHTKLFI
VDGLWSLIGSANLDPRSLRLNFELNLEVYDREFAGTLTKHVAGVLARSREVTLEQMDNRPLPEKLRDGVAKLFSPYL

Sequences:

>Translated_477_residues
MIDELALALGTAALIALSLFSAGHALIMKRDPRSSLGWIVTCLTIPLLGPFFYWGMGVNRIFRRARTWLESGRRLADPEF
FKSTLEQNTATALPPGAEYLSELRRLADRVVSTPLVTGNRFTPLVNGEGAYPAMLEAIAGARRSIHLSTYIFDSDAAGRR
FIDALADAARRGVDVRVIIDGLGEKYSFPRARTLMERTGIRVARFLPLRQGFYMNLRNHRKMLIIDGERAFTGGMNIGDR
HMVERPLPTVVKDLHFAVAGPVVGELQQIFLEDWYFVSGELLDNRHHFPPLPAAGHAISRAIGDGPDKEFRKLFLILMGA
LSCATRQVQIMTPYFIPDRALVAALVTTALRGVEVTLVLPEKNNLPFVHWASRAYLWELLQQGIRVFYQPPPFVHTKLFI
VDGLWSLIGSANLDPRSLRLNFELNLEVYDREFAGTLTKHVAGVLARSREVTLEQMDNRPLPEKLRDGVAKLFSPYL
>Mature_477_residues
MIDELALALGTAALIALSLFSAGHALIMKRDPRSSLGWIVTCLTIPLLGPFFYWGMGVNRIFRRARTWLESGRRLADPEF
FKSTLEQNTATALPPGAEYLSELRRLADRVVSTPLVTGNRFTPLVNGEGAYPAMLEAIAGARRSIHLSTYIFDSDAAGRR
FIDALADAARRGVDVRVIIDGLGEKYSFPRARTLMERTGIRVARFLPLRQGFYMNLRNHRKMLIIDGERAFTGGMNIGDR
HMVERPLPTVVKDLHFAVAGPVVGELQQIFLEDWYFVSGELLDNRHHFPPLPAAGHAISRAIGDGPDKEFRKLFLILMGA
LSCATRQVQIMTPYFIPDRALVAALVTTALRGVEVTLVLPEKNNLPFVHWASRAYLWELLQQGIRVFYQPPPFVHTKLFI
VDGLWSLIGSANLDPRSLRLNFELNLEVYDREFAGTLTKHVAGVLARSREVTLEQMDNRPLPEKLRDGVAKLFSPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=474, Percent_Identity=31.6455696202532, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787007, Length=361, Percent_Identity=31.3019390581717, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87081826, Length=394, Percent_Identity=26.3959390862944, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53502; Mature: 53502

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDELALALGTAALIALSLFSAGHALIMKRDPRSSLGWIVTCLTIPLLGPFFYWGMGVNR
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
IFRRARTWLESGRRLADPEFFKSTLEQNTATALPPGAEYLSELRRLADRVVSTPLVTGNR
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FTPLVNGEGAYPAMLEAIAGARRSIHLSTYIFDSDAAGRRFIDALADAARRGVDVRVIID
CCCEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
GLGEKYSFPRARTLMERTGIRVARFLPLRQGFYMNLRNHRKMLIIDGERAFTGGMNIGDR
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHEECCCCCEEEEEECCEEEECCCCCCCH
HMVERPLPTVVKDLHFAVAGPVVGELQQIFLEDWYFVSGELLDNRHHFPPLPAAGHAISR
HHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECHHCCCCCCCCCCCCCHHHHHH
AIGDGPDKEFRKLFLILMGALSCATRQVQIMTPYFIPDRALVAALVTTALRGVEVTLVLP
HHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEECCHHHHHHHHHHHHHCCEEEEEEEE
EKNNLPFVHWASRAYLWELLQQGIRVFYQPPPFVHTKLFIVDGLWSLIGSANLDPRSLRL
CCCCCCEEEEHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
NFELNLEVYDREFAGTLTKHVAGVLARSREVTLEQMDNRPLPEKLRDGVAKLFSPYL
EEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDELALALGTAALIALSLFSAGHALIMKRDPRSSLGWIVTCLTIPLLGPFFYWGMGVNR
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
IFRRARTWLESGRRLADPEFFKSTLEQNTATALPPGAEYLSELRRLADRVVSTPLVTGNR
HHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
FTPLVNGEGAYPAMLEAIAGARRSIHLSTYIFDSDAAGRRFIDALADAARRGVDVRVIID
CCCEECCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEE
GLGEKYSFPRARTLMERTGIRVARFLPLRQGFYMNLRNHRKMLIIDGERAFTGGMNIGDR
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHEECCCCCEEEEEECCEEEECCCCCCCH
HMVERPLPTVVKDLHFAVAGPVVGELQQIFLEDWYFVSGELLDNRHHFPPLPAAGHAISR
HHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEECHHCCCCCCCCCCCCCHHHHHH
AIGDGPDKEFRKLFLILMGALSCATRQVQIMTPYFIPDRALVAALVTTALRGVEVTLVLP
HHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEECCHHHHHHHHHHHHHCCEEEEEEEE
EKNNLPFVHWASRAYLWELLQQGIRVFYQPPPFVHTKLFIVDGLWSLIGSANLDPRSLRL
CCCCCCEEEEHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
NFELNLEVYDREFAGTLTKHVAGVLARSREVTLEQMDNRPLPEKLRDGVAKLFSPYL
EEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377 [H]