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The map label for this gene is pleD [H]

Identifier: 78221271

GI number: 78221271

Start: 55674

End: 57416

Strand: Direct

Name: pleD [H]

Synonym: Gmet_0043

Alternate gene names: 78221271

Gene position: 55674-57416 (Clockwise)

Preceding gene: 78221270

Following gene: 78221272

Centisome position: 1.39

GC content: 65.23

Gene sequence:

>1743_bases
ATGGCAGGATCACGGAAGATCGTCAGCGCCCGGGAGCTGGACCTCCTGCGCCGCCTTCTCCGGGAACGGACCGAGGAGCT
GGAGCGGGCCAACGAGCGCCTCTTCATGGCCACCCAGGTGAAATCCGAATTCCTCTCCCACATGTCCCGGGAGTTCCAGC
GACCCCTGGACCGCATCGTCGACTTCGCCTCCTGCCTCCGGGACGGCGCCATGGGGGAGGTGAACCCCGAGCAGCGGAGT
GGCCTCGACGCCATCGTCATACGGGGGATGCGGCTCCAGCGGATGCTGCAGCGGGTCCTGGAACTCTGCAGCAGCGATCT
GGGGATGGCGGTCTTTCTTCCCAAGGAGTTTCCCGTCGAGGATGCCCTTGAGCGGGTGGTGAAGCGACTGCGGGAGAGGG
CCGAGAAACAGGGTGTGGCCATCGATGCCGGCTGCGTTGATGGGGGAACCCTCCTGGCCGACGAAGGGAAGTTCTCCTTC
ATTATTGAAGAGTTGGCCACAAATGCCCTCAAGTTTTCACCCCGGGGGTCACGGATCACGGTGCGCCTGCGGAACGTGAC
CGGAGGCCGGGAGGAGCGGTTTCTGGAAGTGGCGGTTTCCGACCAGGGGCCGGGTATCCGGGAGGAGGAGCTGGGGCAGA
TCTTCAAGAGCTTCGAGATGGGGAGCGGCGCGATTGCCAGCCCCGGAAGCCTCGGGCTCGGGCTCGCCCTGGTGAAGCAC
TTCGTGGATCTTCACGGGGGGAGGATATCCGTCGAGAGTCAGGCCGGGGAGGGGAGTTCTTTCACCGTTCTCCTCCCCAA
GGGTGGTCCCTCGGTTCGCATGGTCCGCACGCCCCGGGTCCTCGTGGCCGGTCTGGAGGAGGACTTCGCGGGGCCTCTTC
TTTCGCGGCTACGGGACGAAGGGTATGAAACCATCACTGCCGGCGGCGGTCAGGACGCCCTGGACAAGGGGATTTCCATG
GCCCCGGACCTCTGCATTCTGAGCCTCTCCCTCCCCGAAGTGGGAGGGATCGACGTCTGCCTGCGACTCAAGGCGCACGA
ACGGAGCAAGCAGATCCCGGTCATTATCACCGCCCCCTATCCTGACCGCTCGGCCAAGGTGGGAAGCGCCCAGGCGGGGG
CCGACGGTTTTTTCGCCCTCCCGGCCGAAACGGAGGAGCTCTTCCTCAAGGCCCGCAGCCTCATCGCCCAGAAGCTCAAT
TACGATTTCCTCAAGCGCAATTACGAGATAGCCGCCAGCGAGGCGTTCACCGATCCCCTTACCGGCCTCTTCAACCTGCG
CCAGTTCTGGTTGAGCCTCGACCACGAGCTGGCCCGGGCCCGCCGCTACGGCAGGCACTGTTCCCTCGCCATGATCGACA
TCGATTTCTTCAAGCAGTACAACGACCGGCACGGGCACCTGCGCGGCGACGAGGTGCTGAAGGAGATCAGCAAGCTTTTC
GTTGCCAGCATCAGGAACTCCGACATCGCGGCCCGCTACGGTGGAGAGGAGTTCGTGGTCATCATGCCTGAAACCGGCCG
CGACCTGGCGCTCATCGCCGGGGAAAAGCTGAGGCGGGCCGTGGCCGAGCACCCCTTTTCCCTCCAGGAGACCCAGCCGG
GCGGTCTCCTCACGGTAAGCGTCGGCATCGCCACCTTCCCCGACGACGCCGCGACGGCCCGGGAGATGGTGGATGCGGCG
GACCAGGCCCTCTACCGGGCCAAGGGGCTGGGAAGAGACCGGGTGGAGAGCCGTTCCCCGTAG

Upstream 100 bases:

>100_bases
CTCCGGCCCGGGCCATCCTCCGGGGGAGGGAACCGAAGGGGGGAGAAACCCCCTTCGACCCCCACACGACCCGTTGGCCC
GTGGCCTGACAGGGGGCGTC

Downstream 100 bases:

>100_bases
TCTGTGGCGTCCCGGACGGCGGGTGGTATACTGGAAAGTGTAAGAATACTGCAGGCGATTGGGCGGAAGGCAGGAGGCCT
TCCGGGAAAGGGGTGTGACG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MAGSRKIVSARELDLLRRLLRERTEELERANERLFMATQVKSEFLSHMSREFQRPLDRIVDFASCLRDGAMGEVNPEQRS
GLDAIVIRGMRLQRMLQRVLELCSSDLGMAVFLPKEFPVEDALERVVKRLRERAEKQGVAIDAGCVDGGTLLADEGKFSF
IIEELATNALKFSPRGSRITVRLRNVTGGREERFLEVAVSDQGPGIREEELGQIFKSFEMGSGAIASPGSLGLGLALVKH
FVDLHGGRISVESQAGEGSSFTVLLPKGGPSVRMVRTPRVLVAGLEEDFAGPLLSRLRDEGYETITAGGGQDALDKGISM
APDLCILSLSLPEVGGIDVCLRLKAHERSKQIPVIITAPYPDRSAKVGSAQAGADGFFALPAETEELFLKARSLIAQKLN
YDFLKRNYEIAASEAFTDPLTGLFNLRQFWLSLDHELARARRYGRHCSLAMIDIDFFKQYNDRHGHLRGDEVLKEISKLF
VASIRNSDIAARYGGEEFVVIMPETGRDLALIAGEKLRRAVAEHPFSLQETQPGGLLTVSVGIATFPDDAATAREMVDAA
DQALYRAKGLGRDRVESRSP

Sequences:

>Translated_580_residues
MAGSRKIVSARELDLLRRLLRERTEELERANERLFMATQVKSEFLSHMSREFQRPLDRIVDFASCLRDGAMGEVNPEQRS
GLDAIVIRGMRLQRMLQRVLELCSSDLGMAVFLPKEFPVEDALERVVKRLRERAEKQGVAIDAGCVDGGTLLADEGKFSF
IIEELATNALKFSPRGSRITVRLRNVTGGREERFLEVAVSDQGPGIREEELGQIFKSFEMGSGAIASPGSLGLGLALVKH
FVDLHGGRISVESQAGEGSSFTVLLPKGGPSVRMVRTPRVLVAGLEEDFAGPLLSRLRDEGYETITAGGGQDALDKGISM
APDLCILSLSLPEVGGIDVCLRLKAHERSKQIPVIITAPYPDRSAKVGSAQAGADGFFALPAETEELFLKARSLIAQKLN
YDFLKRNYEIAASEAFTDPLTGLFNLRQFWLSLDHELARARRYGRHCSLAMIDIDFFKQYNDRHGHLRGDEVLKEISKLF
VASIRNSDIAARYGGEEFVVIMPETGRDLALIAGEKLRRAVAEHPFSLQETQPGGLLTVSVGIATFPDDAATAREMVDAA
DQALYRAKGLGRDRVESRSP
>Mature_579_residues
AGSRKIVSARELDLLRRLLRERTEELERANERLFMATQVKSEFLSHMSREFQRPLDRIVDFASCLRDGAMGEVNPEQRSG
LDAIVIRGMRLQRMLQRVLELCSSDLGMAVFLPKEFPVEDALERVVKRLRERAEKQGVAIDAGCVDGGTLLADEGKFSFI
IEELATNALKFSPRGSRITVRLRNVTGGREERFLEVAVSDQGPGIREEELGQIFKSFEMGSGAIASPGSLGLGLALVKHF
VDLHGGRISVESQAGEGSSFTVLLPKGGPSVRMVRTPRVLVAGLEEDFAGPLLSRLRDEGYETITAGGGQDALDKGISMA
PDLCILSLSLPEVGGIDVCLRLKAHERSKQIPVIITAPYPDRSAKVGSAQAGADGFFALPAETEELFLKARSLIAQKLNY
DFLKRNYEIAASEAFTDPLTGLFNLRQFWLSLDHELARARRYGRHCSLAMIDIDFFKQYNDRHGHLRGDEVLKEISKLFV
ASIRNSDIAARYGGEEFVVIMPETGRDLALIAGEKLRRAVAEHPFSLQETQPGGLLTVSVGIATFPDDAATAREMVDAAD
QALYRAKGLGRDRVESRSP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=273, Percent_Identity=29.6703296703297, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693134, Length=168, Percent_Identity=36.3095238095238, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=39.5480225988701, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789149, Length=253, Percent_Identity=29.2490118577075, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788713, Length=352, Percent_Identity=24.4318181818182, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=35.7575757575758, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=30.8900523560209, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI48994928, Length=464, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=345, Percent_Identity=29.8550724637681, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=34.1772151898734, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=26.7241379310345, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788381, Length=165, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787541, Length=163, Percent_Identity=33.1288343558282, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=33.5365853658537, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=261, Percent_Identity=25.2873563218391, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=29.4478527607362, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=31.547619047619, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=29.559748427673, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=257, Percent_Identity=27.6264591439689, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 63858; Mature: 63727

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSRKIVSARELDLLRRLLRERTEELERANERLFMATQVKSEFLSHMSREFQRPLDRIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
DFASCLRDGAMGEVNPEQRSGLDAIVIRGMRLQRMLQRVLELCSSDLGMAVFLPKEFPVE
HHHHHHHCCCCCCCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
DALERVVKRLRERAEKQGVAIDAGCVDGGTLLADEGKFSFIIEELATNALKFSPRGSRIT
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCEEE
VRLRNVTGGREERFLEVAVSDQGPGIREEELGQIFKSFEMGSGAIASPGSLGLGLALVKH
EEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
FVDLHGGRISVESQAGEGSSFTVLLPKGGPSVRMVRTPRVLVAGLEEDFAGPLLSRLRDE
HHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCHHEEECCHHHHHHHHHHHHHHC
GYETITAGGGQDALDKGISMAPDLCILSLSLPEVGGIDVCLRLKAHERSKQIPVIITAPY
CCEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEEHHHCCCCCCEEEECCC
PDRSAKVGSAQAGADGFFALPAETEELFLKARSLIAQKLNYDFLKRNYEIAASEAFTDPL
CCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEHHHHHHHHH
TGLFNLRQFWLSLDHELARARRYGRHCSLAMIDIDFFKQYNDRHGHLRGDEVLKEISKLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHH
VASIRNSDIAARYGGEEFVVIMPETGRDLALIAGEKLRRAVAEHPFSLQETQPGGLLTVS
HHHHCCCCEEEEECCCEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEE
VGIATFPDDAATAREMVDAADQALYRAKGLGRDRVESRSP
ECEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
>Mature Secondary Structure 
AGSRKIVSARELDLLRRLLRERTEELERANERLFMATQVKSEFLSHMSREFQRPLDRIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHH
DFASCLRDGAMGEVNPEQRSGLDAIVIRGMRLQRMLQRVLELCSSDLGMAVFLPKEFPVE
HHHHHHHCCCCCCCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHH
DALERVVKRLRERAEKQGVAIDAGCVDGGTLLADEGKFSFIIEELATNALKFSPRGSRIT
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHEEECCCCCEEE
VRLRNVTGGREERFLEVAVSDQGPGIREEELGQIFKSFEMGSGAIASPGSLGLGLALVKH
EEEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
FVDLHGGRISVESQAGEGSSFTVLLPKGGPSVRMVRTPRVLVAGLEEDFAGPLLSRLRDE
HHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCHHEEECCHHHHHHHHHHHHHHC
GYETITAGGGQDALDKGISMAPDLCILSLSLPEVGGIDVCLRLKAHERSKQIPVIITAPY
CCEEEECCCCHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEEHHHCCCCCCEEEECCC
PDRSAKVGSAQAGADGFFALPAETEELFLKARSLIAQKLNYDFLKRNYEIAASEAFTDPL
CCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCHHHHHCCCCEEHHHHHHHHH
TGLFNLRQFWLSLDHELARARRYGRHCSLAMIDIDFFKQYNDRHGHLRGDEVLKEISKLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHH
VASIRNSDIAARYGGEEFVVIMPETGRDLALIAGEKLRRAVAEHPFSLQETQPGGLLTVS
HHHHCCCCEEEEECCCEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCCEEEEE
VGIATFPDDAATAREMVDAADQALYRAKGLGRDRVESRSP
ECEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA