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The map label for this gene is rnhB

Identifier: 78189946

GI number: 78189946

Start: 2530005

End: 2530625

Strand: Reverse

Name: rnhB

Synonym: Cag_1993

Alternate gene names: 78189946

Gene position: 2530625-2530005 (Counterclockwise)

Preceding gene: 78189949

Following gene: 78189945

Centisome position: 98.39

GC content: 47.99

Gene sequence:

>621_bases
ATGCACACTCATTACGAAGAGCCGCTGTGGCAACACTACGAATTCATTTGCGGTATTGATGAAGTTGGTCGAGGTCCACT
TGCAGGACCAGTTGTTGCGGCTGCCGTTGTTTTCCCTCGCTGGTTTCAGCCAACAGAGGCATTACTGACGTTGCTGAATG
ACTCCAAAAAGCTCTCGGCAAAAGAGCGCGAAAGCTTAGTGCCTGCCATTAAAGCTCAAGCACTCCATTGGGCACTTGCC
GAAGTGCAGCACAACGTGATTGATGAAGTGAACATTTTGCAAGCCACCATGCTTGCTATGAATAACGCCGTTAAAGCACT
GCCCATTATACCATCGCTGCTACTTGTTGATGGCAATCGCTTTACAACCGATCTTGCAATTCCTTACAAAACCATTGTTA
AGGGCGATAGCCATGTTTTTTCTATTGCCGCCGCTTCCGTGTTAGCAAAAGTCCACCGCGATGCCTTGATGTGCGTTTAT
GCAACTCACTACCCACATTATGGTTTTGAACGCCATGCAGGCTATCCAACAAGCGCACACATTGAAGCCATTCGCCAGCA
TGGACGCTGCCCAATACATCGTCAAAGCTTTAAACTGCGCCAACTTGGCGAAAAAGTATGA

Upstream 100 bases:

>100_bases
TATGGATTTAAAGTTATAATCACGCAACCAATATACGGCTATCGTATTAAATTATAGTATCGTAATATTGCAGCATCAAA
AATAATTCCAACCATCCACT

Downstream 100 bases:

>100_bases
ATCCTCCCAATTCAACCTGTGAGCTTGGTCGCCAAGGTGAAGCCTTGGCTGCAACGTATTTGCAAAACGAGGGATACCAA
ATTCTTGAAAGGAATTATCG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MHTHYEEPLWQHYEFICGIDEVGRGPLAGPVVAAAVVFPRWFQPTEALLTLLNDSKKLSAKERESLVPAIKAQALHWALA
EVQHNVIDEVNILQATMLAMNNAVKALPIIPSLLLVDGNRFTTDLAIPYKTIVKGDSHVFSIAAASVLAKVHRDALMCVY
ATHYPHYGFERHAGYPTSAHIEAIRQHGRCPIHRQSFKLRQLGEKV

Sequences:

>Translated_206_residues
MHTHYEEPLWQHYEFICGIDEVGRGPLAGPVVAAAVVFPRWFQPTEALLTLLNDSKKLSAKERESLVPAIKAQALHWALA
EVQHNVIDEVNILQATMLAMNNAVKALPIIPSLLLVDGNRFTTDLAIPYKTIVKGDSHVFSIAAASVLAKVHRDALMCVY
ATHYPHYGFERHAGYPTSAHIEAIRQHGRCPIHRQSFKLRQLGEKV
>Mature_206_residues
MHTHYEEPLWQHYEFICGIDEVGRGPLAGPVVAAAVVFPRWFQPTEALLTLLNDSKKLSAKERESLVPAIKAQALHWALA
EVQHNVIDEVNILQATMLAMNNAVKALPIIPSLLLVDGNRFTTDLAIPYKTIVKGDSHVFSIAAASVLAKVHRDALMCVY
ATHYPHYGFERHAGYPTSAHIEAIRQHGRCPIHRQSFKLRQLGEKV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=191, Percent_Identity=49.2146596858639, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_CHLCH (Q3AP34)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380284.1
- ProteinModelPortal:   Q3AP34
- SMR:   Q3AP34
- STRING:   Q3AP34
- GeneID:   3747103
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1993
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   CCHL340177:CAG_1993-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23014; Mature: 23014

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTHYEEPLWQHYEFICGIDEVGRGPLAGPVVAAAVVFPRWFQPTEALLTLLNDSKKLSA
CCCCCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCH
KERESLVPAIKAQALHWALAEVQHNVIDEVNILQATMLAMNNAVKALPIIPSLLLVDGNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCC
FTTDLAIPYKTIVKGDSHVFSIAAASVLAKVHRDALMCVYATHYPHYGFERHAGYPTSAH
EEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHCCCCCCHHH
IEAIRQHGRCPIHRQSFKLRQLGEKV
HHHHHHCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MHTHYEEPLWQHYEFICGIDEVGRGPLAGPVVAAAVVFPRWFQPTEALLTLLNDSKKLSA
CCCCCCCHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCH
KERESLVPAIKAQALHWALAEVQHNVIDEVNILQATMLAMNNAVKALPIIPSLLLVDGNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCC
FTTDLAIPYKTIVKGDSHVFSIAAASVLAKVHRDALMCVYATHYPHYGFERHAGYPTSAH
EEEEECCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHCCCCCCHHH
IEAIRQHGRCPIHRQSFKLRQLGEKV
HHHHHHCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA