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The map label for this gene is moaA [H]

Identifier: 78189894

GI number: 78189894

Start: 2472805

End: 2473806

Strand: Reverse

Name: moaA [H]

Synonym: Cag_1941

Alternate gene names: 78189894

Gene position: 2473806-2472805 (Counterclockwise)

Preceding gene: 78189895

Following gene: 78189892

Centisome position: 96.18

GC content: 49.7

Gene sequence:

>1002_bases
GTGGATGAGCCGTATGCCACAAGCCAACCGCTTTTTGATACGTTTCAACGCCAAATAACGTATGCGCGCTTAGCGGTTAC
CTCAGCTTGTAACTTGCGCTGCGGCTACTGTTTAAGCGAAGCGCACGAACCCGCTACACTGCACCAACCACTTCTTTCAA
CGGCTGAACTTTGCACCATTATTGAGTTGCTTGCCAAGCATGGCATTCAAAAGCTACGCTTCACGGGTGGAGAACCCTTA
CTGCGTAGCGATATTGTAGCGCTTATTGCTATGGCACGGCAGCACTCATCCATTCGCACCATTGGCTTAACGACGAACGG
CTTGTTGCTTCTTCCCCTTCTCCCTCGTTTACTTGACGCTGGGCTTGACTCGGTAAATCTTAGCCTCGATACACTCAATC
GCCATCGTTACTTTCAAATTACTCGGCGCGACCTTTTTCCGCAAGCTGAAGCGGCGTTGCATGCGCTACTGGCTACACCC
TCGCTTTCAGTAAAATTGAACGTGGTTATGCTGCGTGGCATTAATAGCGATGAACTTACTGGCTTTGTAGAGCTTACCAA
AGAGCATAACATCACTGTGCGCTTTTTAGAGCTGCAACCCTTTGACGACCATCAAATTTGGAAGACAGGGCGCTTTTTGC
GAGCTGATCGGCTTGAAGAGATGTTGCTGCACGCTTATCCCGCTTTGCAGCGCGTGCAAGGCGAAGCAACCCAGCACTTT
AGCTATTGTTTGTCCAACTACAAAGGCGCACTTGCAATTATTCCCGCTTACACAAGAGCATTTTGCGAGCAATGCAACCG
CTTACGTATTACCTCAAGCGGCAAGCTGATAAGTTGCTTGTATGAAAAGGATGGATTAGAACTTTTACCCTTGTTGCGAA
ATGGTGCAAAACCCGAAGAGTTTGCGGCGTTGTTGCAGCAAGCCGTGCTTCGTAAACCAGCCAACGGGCATCAGCGCCAC
ACAGGTGCTGTGCGTACCAGTATGTCGGAGATTGGGGGGTAA

Upstream 100 bases:

>100_bases
GATGTAGAGGCATTTGTTGAGCATTATCCCTCACTGCCATCAACCCTTCGCGTTACTTGTGCCACTGATCAAGTAATGGC
GCTTTATCGGCAATACTACG

Downstream 100 bases:

>100_bases
AATTTCGCTAAAAACGCTTATGAATGGTTGGTGTGCCTAAAATATGCGTAAATTTAGGGACGATAAGAGCAAGTGGTAAT
TATTTGTTCAGCACTTCAAT

Product: Elongator protein 3/MiaB/NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDEPYATSQPLFDTFQRQITYARLAVTSACNLRCGYCLSEAHEPATLHQPLLSTAELCTIIELLAKHGIQKLRFTGGEPL
LRSDIVALIAMARQHSSIRTIGLTTNGLLLLPLLPRLLDAGLDSVNLSLDTLNRHRYFQITRRDLFPQAEAALHALLATP
SLSVKLNVVMLRGINSDELTGFVELTKEHNITVRFLELQPFDDHQIWKTGRFLRADRLEEMLLHAYPALQRVQGEATQHF
SYCLSNYKGALAIIPAYTRAFCEQCNRLRITSSGKLISCLYEKDGLELLPLLRNGAKPEEFAALLQQAVLRKPANGHQRH
TGAVRTSMSEIGG

Sequences:

>Translated_333_residues
MDEPYATSQPLFDTFQRQITYARLAVTSACNLRCGYCLSEAHEPATLHQPLLSTAELCTIIELLAKHGIQKLRFTGGEPL
LRSDIVALIAMARQHSSIRTIGLTTNGLLLLPLLPRLLDAGLDSVNLSLDTLNRHRYFQITRRDLFPQAEAALHALLATP
SLSVKLNVVMLRGINSDELTGFVELTKEHNITVRFLELQPFDDHQIWKTGRFLRADRLEEMLLHAYPALQRVQGEATQHF
SYCLSNYKGALAIIPAYTRAFCEQCNRLRITSSGKLISCLYEKDGLELLPLLRNGAKPEEFAALLQQAVLRKPANGHQRH
TGAVRTSMSEIGG
>Mature_333_residues
MDEPYATSQPLFDTFQRQITYARLAVTSACNLRCGYCLSEAHEPATLHQPLLSTAELCTIIELLAKHGIQKLRFTGGEPL
LRSDIVALIAMARQHSSIRTIGLTTNGLLLLPLLPRLLDAGLDSVNLSLDTLNRHRYFQITRRDLFPQAEAALHALLATP
SLSVKLNVVMLRGINSDELTGFVELTKEHNITVRFLELQPFDDHQIWKTGRFLRADRLEEMLLHAYPALQRVQGEATQHF
SYCLSNYKGALAIIPAYTRAFCEQCNRLRITSSGKLISCLYEKDGLELLPLLRNGAKPEEFAALLQQAVLRKPANGHQRH
TGAVRTSMSEIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=318, Percent_Identity=37.4213836477987, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI22027536, Length=318, Percent_Identity=37.4213836477987, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786999, Length=333, Percent_Identity=34.2342342342342, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32566329, Length=317, Percent_Identity=36.2776025236593, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI32566331, Length=309, Percent_Identity=36.2459546925566, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17561210, Length=318, Percent_Identity=35.5345911949685, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28574488, Length=334, Percent_Identity=35.0299401197605, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI28574490, Length=307, Percent_Identity=35.8306188925081, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37225; Mature: 37225

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEPYATSQPLFDTFQRQITYARLAVTSACNLRCGYCLSEAHEPATLHQPLLSTAELCTI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
IELLAKHGIQKLRFTGGEPLLRSDIVALIAMARQHSSIRTIGLTTNGLLLLPLLPRLLDA
HHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHC
GLDSVNLSLDTLNRHRYFQITRRDLFPQAEAALHALLATPSLSVKLNVVMLRGINSDELT
CCCCEEEEHHHCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHH
GFVELTKEHNITVRFLELQPFDDHQIWKTGRFLRADRLEEMLLHAYPALQRVQGEATQHF
HHEEECCCCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
SYCLSNYKGALAIIPAYTRAFCEQCNRLRITSSGKLISCLYEKDGLELLPLLRNGAKPEE
HHHHHCCCCCEEEEHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCCHHH
FAALLQQAVLRKPANGHQRHTGAVRTSMSEIGG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDEPYATSQPLFDTFQRQITYARLAVTSACNLRCGYCLSEAHEPATLHQPLLSTAELCTI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHH
IELLAKHGIQKLRFTGGEPLLRSDIVALIAMARQHSSIRTIGLTTNGLLLLPLLPRLLDA
HHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHHC
GLDSVNLSLDTLNRHRYFQITRRDLFPQAEAALHALLATPSLSVKLNVVMLRGINSDELT
CCCCEEEEHHHCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHH
GFVELTKEHNITVRFLELQPFDDHQIWKTGRFLRADRLEEMLLHAYPALQRVQGEATQHF
HHEEECCCCCEEEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
SYCLSNYKGALAIIPAYTRAFCEQCNRLRITSSGKLISCLYEKDGLELLPLLRNGAKPEE
HHHHHCCCCCEEEEHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCHHHHHHHCCCCHHH
FAALLQQAVLRKPANGHQRHTGAVRTSMSEIGG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]