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The map label for this gene is ilvI [H]

Identifier: 78189860

GI number: 78189860

Start: 2423177

End: 2424898

Strand: Reverse

Name: ilvI [H]

Synonym: Cag_1905

Alternate gene names: 78189860

Gene position: 2424898-2423177 (Counterclockwise)

Preceding gene: 78189861

Following gene: 78189859

Centisome position: 94.28

GC content: 45.88

Gene sequence:

>1722_bases
ATGCACTCCACTGGTCCTACTCTGACAGGTTCAGAGATATTTTTTGAATGCCTACGACGCGAAAACGTTGAATACATTTT
TGGCTACCCCGGCGGTTCGCTCTTAAAAGTGTATGAAACGCTCCATGACGTAAAAGATATTAAGCACATTCTTGTACGCC
ACGAGCAAGGCGCAACCCATATGGCTGAAGGATATGCTCGCGCAACCGGTAAACCCGGCGTTGTTCTTGTAACCTCAGGT
CCAGGAGCAACCAACACGGTTACCGGCATTACCAATGCTTACATGGACTCTTCGCCCATGATTGTGTTTACAGGACAAGT
TCCAAGCACTCTTATTGGGAACGATGCTTTTCAAGAGGCTGATATTGTTGGTATTACGCGCCCTATCACGAAGCATAATT
TTTTAGTAAAAGATGTTCGTGAACTTGCGCTTACCATTCGCAAAGCCTTTTACTTGGCAACAACAGGGCGACCAGGACCC
GTGCTTATTGACATGCCAAAGGATGTGTTGAATTCATCCTGCCAATTTGAATGGCCTGAAACGGTGGATATTCGTGGCTT
TAAACCAACCGTTAAATGCCATACGCATCAAGTGGAAAAAGCAGCGCAAAAAATTGCCAAAGCGCAACGCCCACTGCTTT
ACATTGGCGGCGGCGTTATTAGCGCTGAAGCCTCAGAAGAGCTTCGCACGCTTGCAACAACCTACAACATTCCTGTAACC
ATGACGTTGCAAGGGCTTGGTGCCTTTCCTGCAACAAATTCACTCAGTATGGGAATGCTTGGAATGCACGGCACCTATTG
GGCTAACCAAGCGGTAAACAAGTGCGACCTCTTAATTGCTATTGGTGCTCGTTTTGATGACCGTGTTACGGGCAAGGTTT
CAACCTTTGCGCCACAAGCCTATAAAATTCATAACGACATTGATCCCACCAATGTTGATAAAAACATTAAGGTTGATTTG
CCTATTGTGGGCGACTCAAAAAACTTCCTCACCACGTTGTTGGAAGTAATGCCTCAGCAACATGAAAATCGTGAGGCGTG
GCTGGCTGAAATTAACGAGTGGCGTAAACAATGTCCGCTTGATTATGGTAAGGATGATGAGCAATTGCGCACAGAATTTG
TGATTGAAGAGGTGTCGAAGCAAACGGCAGGCAATGCGGTTGTGGTTACCGATGTGGGGCAGCACCAAATGTGGACATCA
CAGTTTTACAGCTTTGTAAACCCTCGTTCCATTATTACAAGCGGTGGACTTGGAACTATGGGCTACGGCTTACCTGCCGC
CATTGGTGCCGCATTTGGCATACACAATAAGCCTGTGGTACTTTTTTGTGGCGATGGTGGTTTTATGATGAATATTCAAG
AGTTAGTTACAGCCGTGCATCATAAAATTCCCATTAAAATCTTTTTAATTAACAACTGCTATCTTGGTATGGTTCGCCAA
TGGCAGGAGCTGTTCCATGAAGAAAAATATACCTTTACCGACTTAGCAGAGAGTAATCCTGATTTTGTAAAGGTTGCCGA
AGCTTTTGGCTGCAAAGCGTTGCGTGCCGAAACTCCCGATGCTGCTCGCCAAGCAATTAGCGAAGCTCTTGCATGGAATG
AGGGACCTGTGTTGGTTGAGTGTAAAGTGGTACGTAAAGATATGGTTTTCCCGATGGTTCCAGCAGGCGGCTCCATTTCG
GATATGTTGCTTGCCCGCTTAAACCCTAAAACAATGGTGTAA

Upstream 100 bases:

>100_bases
TGCGCTCCGTCCGCCTTGCTCTCAAAGCGCTCATAACAATTTTTAAAGATGCCCTCTTATGAGCCCAACATCTTGCAAAA
CCAACTAAAACCTTTGACCT

Downstream 100 bases:

>100_bases
GTATTGTTTTGCCTTGTGGGTTCTCCACACTGCTTGCTTGAAAAAGGTTGAAACCTCAAGAATTATTTAAAAGAGAGAGC
CATGCTGAATGTTACAGAGA

Product: acetolactate synthase large subunit biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MHSTGPTLTGSEIFFECLRRENVEYIFGYPGGSLLKVYETLHDVKDIKHILVRHEQGATHMAEGYARATGKPGVVLVTSG
PGATNTVTGITNAYMDSSPMIVFTGQVPSTLIGNDAFQEADIVGITRPITKHNFLVKDVRELALTIRKAFYLATTGRPGP
VLIDMPKDVLNSSCQFEWPETVDIRGFKPTVKCHTHQVEKAAQKIAKAQRPLLYIGGGVISAEASEELRTLATTYNIPVT
MTLQGLGAFPATNSLSMGMLGMHGTYWANQAVNKCDLLIAIGARFDDRVTGKVSTFAPQAYKIHNDIDPTNVDKNIKVDL
PIVGDSKNFLTTLLEVMPQQHENREAWLAEINEWRKQCPLDYGKDDEQLRTEFVIEEVSKQTAGNAVVVTDVGQHQMWTS
QFYSFVNPRSIITSGGLGTMGYGLPAAIGAAFGIHNKPVVLFCGDGGFMMNIQELVTAVHHKIPIKIFLINNCYLGMVRQ
WQELFHEEKYTFTDLAESNPDFVKVAEAFGCKALRAETPDAARQAISEALAWNEGPVLVECKVVRKDMVFPMVPAGGSIS
DMLLARLNPKTMV

Sequences:

>Translated_573_residues
MHSTGPTLTGSEIFFECLRRENVEYIFGYPGGSLLKVYETLHDVKDIKHILVRHEQGATHMAEGYARATGKPGVVLVTSG
PGATNTVTGITNAYMDSSPMIVFTGQVPSTLIGNDAFQEADIVGITRPITKHNFLVKDVRELALTIRKAFYLATTGRPGP
VLIDMPKDVLNSSCQFEWPETVDIRGFKPTVKCHTHQVEKAAQKIAKAQRPLLYIGGGVISAEASEELRTLATTYNIPVT
MTLQGLGAFPATNSLSMGMLGMHGTYWANQAVNKCDLLIAIGARFDDRVTGKVSTFAPQAYKIHNDIDPTNVDKNIKVDL
PIVGDSKNFLTTLLEVMPQQHENREAWLAEINEWRKQCPLDYGKDDEQLRTEFVIEEVSKQTAGNAVVVTDVGQHQMWTS
QFYSFVNPRSIITSGGLGTMGYGLPAAIGAAFGIHNKPVVLFCGDGGFMMNIQELVTAVHHKIPIKIFLINNCYLGMVRQ
WQELFHEEKYTFTDLAESNPDFVKVAEAFGCKALRAETPDAARQAISEALAWNEGPVLVECKVVRKDMVFPMVPAGGSIS
DMLLARLNPKTMV
>Mature_573_residues
MHSTGPTLTGSEIFFECLRRENVEYIFGYPGGSLLKVYETLHDVKDIKHILVRHEQGATHMAEGYARATGKPGVVLVTSG
PGATNTVTGITNAYMDSSPMIVFTGQVPSTLIGNDAFQEADIVGITRPITKHNFLVKDVRELALTIRKAFYLATTGRPGP
VLIDMPKDVLNSSCQFEWPETVDIRGFKPTVKCHTHQVEKAAQKIAKAQRPLLYIGGGVISAEASEELRTLATTYNIPVT
MTLQGLGAFPATNSLSMGMLGMHGTYWANQAVNKCDLLIAIGARFDDRVTGKVSTFAPQAYKIHNDIDPTNVDKNIKVDL
PIVGDSKNFLTTLLEVMPQQHENREAWLAEINEWRKQCPLDYGKDDEQLRTEFVIEEVSKQTAGNAVVVTDVGQHQMWTS
QFYSFVNPRSIITSGGLGTMGYGLPAAIGAAFGIHNKPVVLFCGDGGFMMNIQELVTAVHHKIPIKIFLINNCYLGMVRQ
WQELFHEEKYTFTDLAESNPDFVKVAEAFGCKALRAETPDAARQAISEALAWNEGPVLVECKVVRKDMVFPMVPAGGSIS
DMLLARLNPKTMV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=579, Percent_Identity=26.4248704663212, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI21361361, Length=562, Percent_Identity=24.9110320284698, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=45.2464788732394, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=42.5531914893617, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=33.6996336996337, Blast_Score=307, Evalue=2e-84,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=26.3837638376384, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1788716, Length=580, Percent_Identity=25.1724137931034, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=579, Percent_Identity=25.2158894645941, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=558, Percent_Identity=24.910394265233, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=558, Percent_Identity=24.910394265233, Blast_Score=133, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=45.0777202072539, Blast_Score=490, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320816, Length=523, Percent_Identity=25.8126195028681, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321524, Length=493, Percent_Identity=23.3265720081136, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=489, Percent_Identity=23.5173824130879, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=528, Percent_Identity=21.5909090909091, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=482, Percent_Identity=27.1784232365145, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63041; Mature: 63041

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSTGPTLTGSEIFFECLRRENVEYIFGYPGGSLLKVYETLHDVKDIKHILVRHEQGATH
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGVVLVTSGPGATNTVTGITNAYMDSSPMIVFTGQVPSTLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCHHHCCCCCCCC
DIVGITRPITKHNFLVKDVRELALTIRKAFYLATTGRPGPVLIDMPKDVLNSSCQFEWPE
CEEEEECCCHHCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCHHHCCCCCCCCCCC
TVDIRGFKPTVKCHTHQVEKAAQKIAKAQRPLLYIGGGVISAEASEELRTLATTYNIPVT
CEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHHEECCCEE
MTLQGLGAFPATNSLSMGMLGMHGTYWANQAVNKCDLLIAIGARFDDRVTGKVSTFAPQA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCCE
YKIHNDIDPTNVDKNIKVDLPIVGDSKNFLTTLLEVMPQQHENREAWLAEINEWRKQCPL
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
DYGKDDEQLRTEFVIEEVSKQTAGNAVVVTDVGQHQMWTSQFYSFVNPRSIITSGGLGTM
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHEECCCCCCC
GYGLPAAIGAAFGIHNKPVVLFCGDGGFMMNIQELVTAVHHKIPIKIFLINNCYLGMVRQ
CCCHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQELFHEEKYTFTDLAESNPDFVKVAEAFGCKALRAETPDAARQAISEALAWNEGPVLVE
HHHHHHCCCCEEHHHCCCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEE
CKVVRKDMVFPMVPAGGSISDMLLARLNPKTMV
EEEEHHCCCCEECCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHSTGPTLTGSEIFFECLRRENVEYIFGYPGGSLLKVYETLHDVKDIKHILVRHEQGATH
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
MAEGYARATGKPGVVLVTSGPGATNTVTGITNAYMDSSPMIVFTGQVPSTLIGNDAFQEA
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCCHHHCCCCCCCC
DIVGITRPITKHNFLVKDVRELALTIRKAFYLATTGRPGPVLIDMPKDVLNSSCQFEWPE
CEEEEECCCHHCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECCHHHCCCCCCCCCCC
TVDIRGFKPTVKCHTHQVEKAAQKIAKAQRPLLYIGGGVISAEASEELRTLATTYNIPVT
CEECCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEECCEEEECCHHHHHHHHHHEECCCEE
MTLQGLGAFPATNSLSMGMLGMHGTYWANQAVNKCDLLIAIGARFDDRVTGKVSTFAPQA
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCEECCCCCE
YKIHNDIDPTNVDKNIKVDLPIVGDSKNFLTTLLEVMPQQHENREAWLAEINEWRKQCPL
EEECCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
DYGKDDEQLRTEFVIEEVSKQTAGNAVVVTDVGQHQMWTSQFYSFVNPRSIITSGGLGTM
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHEECCCCCCC
GYGLPAAIGAAFGIHNKPVVLFCGDGGFMMNIQELVTAVHHKIPIKIFLINNCYLGMVRQ
CCCHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECCCHHHHHHH
WQELFHEEKYTFTDLAESNPDFVKVAEAFGCKALRAETPDAARQAISEALAWNEGPVLVE
HHHHHHCCCCEEHHHCCCCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHCCCCCCEEEE
CKVVRKDMVFPMVPAGGSISDMLLARLNPKTMV
EEEEHHCCCCEECCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]