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The map label for this gene is clpB2 [H]

Identifier: 78189831

GI number: 78189831

Start: 2384981

End: 2386330

Strand: Reverse

Name: clpB2 [H]

Synonym: Cag_1876

Alternate gene names: 78189831

Gene position: 2386330-2384981 (Counterclockwise)

Preceding gene: 78189832

Following gene: 78189830

Centisome position: 92.78

GC content: 47.63

Gene sequence:

>1350_bases
ATGAGGGCGCAAAACCTCGCTAATACTATGCAATTCGATCCTAATAAATTTACCCTGAAAGCTCAGGAGGCTTTGCAAGC
TGCCGCAACGCTTGCCAGCAGTAATCAGCATCAGCAAATTGAGCCACTCCATTTGTTGCTTGCTATGTTTCAAGATAAGC
AGAGTATTGCGGTGCAAATTGCTCAAAAGCTTGAAGCTTCGCCCGATATGTTGCTTGCGGCGCTTGAGCGAGAGCTTGAG
CGGTTGCCTCGTGTAACGGGGGCTTCGGCAAGTGGGCAATATATTTCGCAACCTCTTGGTAAGGTGTTTGATGTTGCCTT
AAAAGAGGCGGAAAATCTTAAAGATGACTACATCAGTTCCGAGCATTTACTGATTGCTTTAAGTGAAGCAGGTGTGGCAA
TTTCGCCCATATTGCGAGATGCAGGGTTTAATCGCGATGCTATGCTCAAGGTGCTTGCCACTATTCGTGGTACGCAGCGG
GTGAGTAGCCAAAATGCTGAAGAAACCTACAATTCGCTGAAAAAATATTCTCGTAACCTTAACGACCAAGCTCGACGTGG
TGGGCTTGATCCTGTTATTGGGCGCGATGATGAAATTCGTCGGGTGTTGCAAATTCTTAGCCGTCGCACCAAAAACAATC
CTGTGCTGATTGGTGAGCCGGGTGTGGGCAAAACGGCGATTGTAGAAGGGATTGCGCAGCGCATTGTTGCGGGCGATGTG
CCTGAAAATCTTAAAAGCAAACATATTGCGGCGCTTGATATTGCACAACTTGTTGCGGGTGCCAAGTTTCGTGGCGAGTT
TGAAGAGCGCTTAAAAGCGGTGGTTAAAGAGGTGCAATCTTCTGAGGGTGAAATCATTCTTTTTATTGATGAATTGCATC
TGTTAGTTGGAGCTGGTTCGGCTGAAGGTTCAATGGATGCTGCCAACATTTTAAAGCCCGCGCTTGCACGCGGTGAGTTG
CGCTGTATTGGTGCAACCACGCTTGATGAGTATCGCAAGCATATTGAAAAAGATGCAGCCCTTGAACGTCGCTTTCAAAC
GGTGGTGGTTGATCAGCCCAACGTGCAAGATACTGTTTCAATTTTGCGCGGTTTAAAAGAGAAGTATGAAATTCACCACG
GGGTGCGCATTAAAGATGCGGCATTGGTGGCGGCGGCAGAGCTGTCAAATCGTTACATTGCCGATCGCTTTTTGCCCGAT
AAGGCAATTGATTTAATTGATGAAGCCTCGTCGCGTTTGCGGCTTGAAATTGATAGCAGCCCCGAAGAACTTGATCGCTT
AAATCGCGAAATTCGTCGTCTTGAGGTTGAGCGTGAGGCACTCAAACGCGAGTTGGAACCGGGTAGTTAA

Upstream 100 bases:

>100_bases
AAACATGCCTTCGCCATTGGATGATGATAAATCAACTTCTCGTGAATAAAATTCATTGCGATTGATTAAGTAGGGGAAGT
GATTGTTAAACGATGGCTTA

Downstream 100 bases:

>100_bases
TTGCTGTTCGATTTTTTTGCACAAGAAGTCCCCATTTATGGAGAGCATCATCGCATGAGCATTCAGTTTGTAAAACATAA
CCCCGCTTTTCTTGATGCTG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRAQNLANTMQFDPNKFTLKAQEALQAAATLASSNQHQQIEPLHLLLAMFQDKQSIAVQIAQKLEASPDMLLAALERELE
RLPRVTGASASGQYISQPLGKVFDVALKEAENLKDDYISSEHLLIALSEAGVAISPILRDAGFNRDAMLKVLATIRGTQR
VSSQNAEETYNSLKKYSRNLNDQARRGGLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVAGDV
PENLKSKHIAALDIAQLVAGAKFRGEFEERLKAVVKEVQSSEGEIILFIDELHLLVGAGSAEGSMDAANILKPALARGEL
RCIGATTLDEYRKHIEKDAALERRFQTVVVDQPNVQDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPD
KAIDLIDEASSRLRLEIDSSPEELDRLNREIRRLEVEREALKRELEPGS

Sequences:

>Translated_449_residues
MRAQNLANTMQFDPNKFTLKAQEALQAAATLASSNQHQQIEPLHLLLAMFQDKQSIAVQIAQKLEASPDMLLAALERELE
RLPRVTGASASGQYISQPLGKVFDVALKEAENLKDDYISSEHLLIALSEAGVAISPILRDAGFNRDAMLKVLATIRGTQR
VSSQNAEETYNSLKKYSRNLNDQARRGGLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVAGDV
PENLKSKHIAALDIAQLVAGAKFRGEFEERLKAVVKEVQSSEGEIILFIDELHLLVGAGSAEGSMDAANILKPALARGEL
RCIGATTLDEYRKHIEKDAALERRFQTVVVDQPNVQDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPD
KAIDLIDEASSRLRLEIDSSPEELDRLNREIRRLEVEREALKRELEPGS
>Mature_449_residues
MRAQNLANTMQFDPNKFTLKAQEALQAAATLASSNQHQQIEPLHLLLAMFQDKQSIAVQIAQKLEASPDMLLAALERELE
RLPRVTGASASGQYISQPLGKVFDVALKEAENLKDDYISSEHLLIALSEAGVAISPILRDAGFNRDAMLKVLATIRGTQR
VSSQNAEETYNSLKKYSRNLNDQARRGGLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVAGDV
PENLKSKHIAALDIAQLVAGAKFRGEFEERLKAVVKEVQSSEGEIILFIDELHLLVGAGSAEGSMDAANILKPALARGEL
RCIGATTLDEYRKHIEKDAALERRFQTVVVDQPNVQDTVSILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPD
KAIDLIDEASSRLRLEIDSSPEELDRLNREIRRLEVEREALKRELEPGS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Escherichia coli, GI1788943, Length=435, Percent_Identity=54.4827586206897, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1787109, Length=412, Percent_Identity=40.5339805825243, Blast_Score=275, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=278, Percent_Identity=63.3093525179856, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323002, Length=438, Percent_Identity=45.8904109589041, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF02861 Clp_N [H]

EC number: NA

Molecular weight: Translated: 49659; Mature: 49659

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQNLANTMQFDPNKFTLKAQEALQAAATLASSNQHQQIEPLHLLLAMFQDKQSIAVQI
CCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHH
AQKLEASPDMLLAALERELERLPRVTGASASGQYISQPLGKVFDVALKEAENLKDDYISS
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCC
EHLLIALSEAGVAISPILRDAGFNRDAMLKVLATIRGTQRVSSQNAEETYNSLKKYSRNL
CCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
NDQARRGGLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVAGDV
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
PENLKSKHIAALDIAQLVAGAKFRGEFEERLKAVVKEVQSSEGEIILFIDELHLLVGAGS
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCC
AEGSMDAANILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVVVDQPNVQDTVS
CCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
ILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEASSRLRLEIDSS
HHHHHHHHHHHHCCCEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCEEEEECCC
PEELDRLNREIRRLEVEREALKRELEPGS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRAQNLANTMQFDPNKFTLKAQEALQAAATLASSNQHQQIEPLHLLLAMFQDKQSIAVQI
CCCHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHH
AQKLEASPDMLLAALERELERLPRVTGASASGQYISQPLGKVFDVALKEAENLKDDYISS
HHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCC
EHLLIALSEAGVAISPILRDAGFNRDAMLKVLATIRGTQRVSSQNAEETYNSLKKYSRNL
CCEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
NDQARRGGLDPVIGRDDEIRRVLQILSRRTKNNPVLIGEPGVGKTAIVEGIAQRIVAGDV
CHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
PENLKSKHIAALDIAQLVAGAKFRGEFEERLKAVVKEVQSSEGEIILFIDELHLLVGAGS
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCC
AEGSMDAANILKPALARGELRCIGATTLDEYRKHIEKDAALERRFQTVVVDQPNVQDTVS
CCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
ILRGLKEKYEIHHGVRIKDAALVAAAELSNRYIADRFLPDKAIDLIDEASSRLRLEIDSS
HHHHHHHHHHHHCCCEEHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHCCCCEEEEECCC
PEELDRLNREIRRLEVEREALKRELEPGS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]