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The map label for this gene is gatB

Identifier: 78189828

GI number: 78189828

Start: 2380313

End: 2381740

Strand: Reverse

Name: gatB

Synonym: Cag_1873

Alternate gene names: 78189828

Gene position: 2381740-2380313 (Counterclockwise)

Preceding gene: 78189829

Following gene: 78189826

Centisome position: 92.6

GC content: 46.15

Gene sequence:

>1428_bases
ATGGATTATGAATTAGTTGTGGGCCTCGAGGTTCACTGCCAGCTTAATACGGTTACCAAAGCTTTTTGTGGCTGCTCTGC
CCAATTTGGTAAAGCGGCAAATACCAACGTTTGCCCTGTCTGCCTTGCTTTGCCGGGCGCTTTGCCTGTGCTGAATCGCC
AAGTGGTAGAAGATGCTGTAAAAATTGGCTTAGCGCTTGACTGCCGCATTGCTCCCCATTCAGTGCTTGCCCGTAAAAAT
TATTTTTACCCCGATCTTCCTAAGGGTTATCAAATTTCTCAGTTTGAAGAACCAATTTGCAGTGATGGCTCTATTGATGT
TGAGCTTGATGGTGTAAATCGCACCATTCACTTAATTCGTATTCATATTGAGGAAGATGCAGGTAAGTCCATTCACGATA
TTGGCGACGATACCTTTATTGATTTAAATCGTAGTGGTGTGCCGCTGCTTGAAATTGTTAGCTATCCCGATATTCGCTCG
GCAAAAGAAGCCTCAGCCTACCTACAAAAGCTTCGCCAAATTGTAAAATATCTTGGCATTTCCGATGGCAACATGGAGGA
GGGAAGCCTTCGCTGTGATGCAAACGTGTCGCTCCGCCCAGTGGGCGCAACTGAGTATGGGACGCGTACCGAAATTAAGA
ATATGAACTCCTTTAAAAACGTTGAAAAAGCCATTGAATATGAGGCTTTGCGTCATCGGGAGATTCTTGAAAATGGTGGC
GTTATTATCCAAGAAACCCGTTTGTGGGATGCCGATAAAGGTGAAACGCGCTCCATGCGCGGCAAAGAATTTGCGCACGA
TTATCGCTACTTCCCTGATCCCGATTTAGTGCCCGTCTTAGTGGATGAGGCAATGATTGAGCGCTTAAAGCTTGAGTTGC
CCGAATTCCCCGAAATGCGTGCTCGCCGTTTTGCCGCAGACTATGGAATTCCCACTTATGATGCAGGCGTGCTTACCGTA
GAGCGTGAACTTGCTGATTATTTTGAAGAAACGGTAAAGCTATCGGGCGATGCTAAAACATCCTCCAATTGGGTGATGGG
CGAAGTAATGCGCACGTTAAAAGAGAAGTATCTTGATATTGCGGAATTCTCAATTCGTCCCGCACGTTTGGCGGGTTTAA
TTCAGCTTATCCATAACAAAGTTATTAGCAACACCATTGCAAAGCAGGTGTTTGAAGTTATGCTGAACGATGAGGCAGAG
CCAGCCGCAATTGTGGAGCGTGATGGCTTAGCGCAGGTGTCGGATAGCGGAGCGCTTGAAGCAGTTGCGCAAGAGGTGAT
TGATGCCAACCCCAAGCAATTAGCCGAATATCGTGAAGGCAAAACCAAACTGATGGGCTTTTTTGTAGGGCAGTGCATGA
GCCGTATGAAAGGCAAAGCCAATCCTCAGCTTGTGAACGATATTTTATTGAAAAAGTTAGAAGGGTAA

Upstream 100 bases:

>100_bases
CTGCTTTCAACGGAGCAGTTGTTTCGGCGCTTGCTTTGCCCTTTTTCTTTCATAACAAGGGCATGTATCATCTTATTTTT
TAAACACTCCTGTTCTTCCA

Downstream 100 bases:

>100_bases
GCTAACGTTAGCTGTTACCAGCAAAAGCTTGTGTTGTTATGGTTCCCATTCCAGCACAAGCTTTTGCTGTTTAAAAATGT
ATTTTAACCCTCGTTAGTTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MDYELVVGLEVHCQLNTVTKAFCGCSAQFGKAANTNVCPVCLALPGALPVLNRQVVEDAVKIGLALDCRIAPHSVLARKN
YFYPDLPKGYQISQFEEPICSDGSIDVELDGVNRTIHLIRIHIEEDAGKSIHDIGDDTFIDLNRSGVPLLEIVSYPDIRS
AKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSLRPVGATEYGTRTEIKNMNSFKNVEKAIEYEALRHREILENGG
VIIQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDEAMIERLKLELPEFPEMRARRFAADYGIPTYDAGVLTV
ERELADYFEETVKLSGDAKTSSNWVMGEVMRTLKEKYLDIAEFSIRPARLAGLIQLIHNKVISNTIAKQVFEVMLNDEAE
PAAIVERDGLAQVSDSGALEAVAQEVIDANPKQLAEYREGKTKLMGFFVGQCMSRMKGKANPQLVNDILLKKLEG

Sequences:

>Translated_475_residues
MDYELVVGLEVHCQLNTVTKAFCGCSAQFGKAANTNVCPVCLALPGALPVLNRQVVEDAVKIGLALDCRIAPHSVLARKN
YFYPDLPKGYQISQFEEPICSDGSIDVELDGVNRTIHLIRIHIEEDAGKSIHDIGDDTFIDLNRSGVPLLEIVSYPDIRS
AKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSLRPVGATEYGTRTEIKNMNSFKNVEKAIEYEALRHREILENGG
VIIQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDEAMIERLKLELPEFPEMRARRFAADYGIPTYDAGVLTV
ERELADYFEETVKLSGDAKTSSNWVMGEVMRTLKEKYLDIAEFSIRPARLAGLIQLIHNKVISNTIAKQVFEVMLNDEAE
PAAIVERDGLAQVSDSGALEAVAQEVIDANPKQLAEYREGKTKLMGFFVGQCMSRMKGKANPQLVNDILLKKLEG
>Mature_475_residues
MDYELVVGLEVHCQLNTVTKAFCGCSAQFGKAANTNVCPVCLALPGALPVLNRQVVEDAVKIGLALDCRIAPHSVLARKN
YFYPDLPKGYQISQFEEPICSDGSIDVELDGVNRTIHLIRIHIEEDAGKSIHDIGDDTFIDLNRSGVPLLEIVSYPDIRS
AKEASAYLQKLRQIVKYLGISDGNMEEGSLRCDANVSLRPVGATEYGTRTEIKNMNSFKNVEKAIEYEALRHREILENGG
VIIQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDEAMIERLKLELPEFPEMRARRFAADYGIPTYDAGVLTV
ERELADYFEETVKLSGDAKTSSNWVMGEVMRTLKEKYLDIAEFSIRPARLAGLIQLIHNKVISNTIAKQVFEVMLNDEAE
PAAIVERDGLAQVSDSGALEAVAQEVIDANPKQLAEYREGKTKLMGFFVGQCMSRMKGKANPQLVNDILLKKLEG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=36.8421052631579, Blast_Score=311, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=380, Percent_Identity=35.5263157894737, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=31.2252964426877, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI45550808, Length=482, Percent_Identity=34.0248962655602, Blast_Score=288, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CHLCH (Q3APF2)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380166.1
- ProteinModelPortal:   Q3APF2
- SMR:   Q3APF2
- STRING:   Q3APF2
- GeneID:   3747025
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1873
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CCHL340177:CAG_1873-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52993; Mature: 52993

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYELVVGLEVHCQLNTVTKAFCGCSAQFGKAANTNVCPVCLALPGALPVLNRQVVEDAV
CCCEEEEEEEEEEEHHHHHHHHHCCCHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KIGLALDCRIAPHSVLARKNYFYPDLPKGYQISQFEEPICSDGSIDVELDGVNRTIHLIR
HHCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCEEEEEE
IHIEEDAGKSIHDIGDDTFIDLNRSGVPLLEIVSYPDIRSAKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSLRPVGATEYGTRTEIKNMNSFKNVEKAIEYEALRHREILENGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
VIIQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDEAMIERLKLELPEFPEMR
EEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
ARRFAADYGIPTYDAGVLTVERELADYFEETVKLSGDAKTSSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AEFSIRPARLAGLIQLIHNKVISNTIAKQVFEVMLNDEAEPAAIVERDGLAQVSDSGALE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCCCCHHHH
AVAQEVIDANPKQLAEYREGKTKLMGFFVGQCMSRMKGKANPQLVNDILLKKLEG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYELVVGLEVHCQLNTVTKAFCGCSAQFGKAANTNVCPVCLALPGALPVLNRQVVEDAV
CCCEEEEEEEEEEEHHHHHHHHHCCCHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
KIGLALDCRIAPHSVLARKNYFYPDLPKGYQISQFEEPICSDGSIDVELDGVNRTIHLIR
HHCEEEEEEECCHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEECCCCCEEEEEE
IHIEEDAGKSIHDIGDDTFIDLNRSGVPLLEIVSYPDIRSAKEASAYLQKLRQIVKYLGI
EEEECCCCCHHHHCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
SDGNMEEGSLRCDANVSLRPVGATEYGTRTEIKNMNSFKNVEKAIEYEALRHREILENGG
CCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
VIIQETRLWDADKGETRSMRGKEFAHDYRYFPDPDLVPVLVDEAMIERLKLELPEFPEMR
EEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
ARRFAADYGIPTYDAGVLTVERELADYFEETVKLSGDAKTSSNWVMGEVMRTLKEKYLDI
HHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AEFSIRPARLAGLIQLIHNKVISNTIAKQVFEVMLNDEAEPAAIVERDGLAQVSDSGALE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCCCCCHHHH
AVAQEVIDANPKQLAEYREGKTKLMGFFVGQCMSRMKGKANPQLVNDILLKKLEG
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA