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The map label for this gene is rpoA

Identifier: 78189780

GI number: 78189780

Start: 2345498

End: 2346481

Strand: Reverse

Name: rpoA

Synonym: Cag_1824

Alternate gene names: 78189780

Gene position: 2346481-2345498 (Counterclockwise)

Preceding gene: 78189781

Following gene: 78189779

Centisome position: 91.23

GC content: 39.94

Gene sequence:

>984_bases
ATGATATACCAAATGCAGATGCCTGCAAAAATAGAGCTTGACGAATCTTCTCATAGTGATAGTTTCGGGAAGTTTATCGC
CCAACCGCTTGAGCGTGGTTATGGCGTTACTCTTGGCAACTTGATGAGAAGAGTGTTGCTTGCCTCGTTACCGGGAACTG
CAATTACAGGTATCAAAATAGAGAATGTTTATCATGAGTTCTCTACTATTCAGGGCGTTCGCGAAGATGTTCCTGAAATT
GTGTTAAATCTTAAAAAGGTTCGCTTTCGATCACAATGTAAGCGTAGTTGCAAAACCACGGTAACATTGGTTGGTCCTAT
GGAATTTACCGCAGGTGTTATTCAGCCGCAAGAAGGTGAGTTCGAGGTTCTTAATAAGGATTTACATATTGCGACTATCA
ATGCGGGTACAACCGTTACGCTTGATATTTTTATAGGACGTGGTCGTGGTTATGTGCCTGCTGAAGAAAATCGTGCTGAA
GGAATGCCGCTTGGGTTTATTCCAATTGACTCGATTTTTACCCCTATTCGTAATGTAAAGTTTACGGTTGAAAATACTCG
TGTGGGGCAGCGTACTGATTATGAAAAAATGATTCTTGAGGTTGAGACTGATGGTTCAATTACTCCTGATGATTCCATTA
GTTTAGCAGGAAGAGTTATTTCTGATCATGTTTTACTTTTTGCTGATTTCTCTCCTGCTGAAGAGGAATACACAGAAGAA
GAGTTCAAGCAGCAAGATGATGAGTTTGAAACGATGCGTCGTTTGTTAGCAACAAAAATCGAAGATCTTGATTTATCGGT
TCGCTCACACAATTGCTTGCGTCTTGCTGAAATTGATACGCTTGGAGAGTTAGTTTCGCATAAGGAAGATGAGTTGTTGA
ATTACAAAAACTTTGGTAAGAAGTCGCTTACCGAGCTTAAAGAGCAACTTGATAAGTTTGATCTTAAGTTTGGTATGGAT
ATTACCCGTTACCAAATGAAGTAA

Upstream 100 bases:

>100_bases
TGTGCCTGAAAGAGCCGATATCCAAGAAGAGTTCAACGAGCAGTTAGTTGTTGAGTTGTACTCTAAGTGATTTTATTGAA
AATATTAAGGTATAAGTGAT

Downstream 100 bases:

>100_bases
GAGTAAAAAGTTTTATTGAATTTTTGAGATTAGGATAAATCATGCGTAAAGGAAAGCCTGCACGTAAACTTGGTAGAACC
GCCGCTCATAGAAGAGCAAC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIYQMQMPAKIELDESSHSDSFGKFIAQPLERGYGVTLGNLMRRVLLASLPGTAITGIKIENVYHEFSTIQGVREDVPEI
VLNLKKVRFRSQCKRSCKTTVTLVGPMEFTAGVIQPQEGEFEVLNKDLHIATINAGTTVTLDIFIGRGRGYVPAEENRAE
GMPLGFIPIDSIFTPIRNVKFTVENTRVGQRTDYEKMILEVETDGSITPDDSISLAGRVISDHVLLFADFSPAEEEYTEE
EFKQQDDEFETMRRLLATKIEDLDLSVRSHNCLRLAEIDTLGELVSHKEDELLNYKNFGKKSLTELKEQLDKFDLKFGMD
ITRYQMK

Sequences:

>Translated_327_residues
MIYQMQMPAKIELDESSHSDSFGKFIAQPLERGYGVTLGNLMRRVLLASLPGTAITGIKIENVYHEFSTIQGVREDVPEI
VLNLKKVRFRSQCKRSCKTTVTLVGPMEFTAGVIQPQEGEFEVLNKDLHIATINAGTTVTLDIFIGRGRGYVPAEENRAE
GMPLGFIPIDSIFTPIRNVKFTVENTRVGQRTDYEKMILEVETDGSITPDDSISLAGRVISDHVLLFADFSPAEEEYTEE
EFKQQDDEFETMRRLLATKIEDLDLSVRSHNCLRLAEIDTLGELVSHKEDELLNYKNFGKKSLTELKEQLDKFDLKFGMD
ITRYQMK
>Mature_327_residues
MIYQMQMPAKIELDESSHSDSFGKFIAQPLERGYGVTLGNLMRRVLLASLPGTAITGIKIENVYHEFSTIQGVREDVPEI
VLNLKKVRFRSQCKRSCKTTVTLVGPMEFTAGVIQPQEGEFEVLNKDLHIATINAGTTVTLDIFIGRGRGYVPAEENRAE
GMPLGFIPIDSIFTPIRNVKFTVENTRVGQRTDYEKMILEVETDGSITPDDSISLAGRVISDHVLLFADFSPAEEEYTEE
EFKQQDDEFETMRRLLATKIEDLDLSVRSHNCLRLAEIDTLGELVSHKEDELLNYKNFGKKSLTELKEQLDKFDLKFGMD
ITRYQMK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=312, Percent_Identity=37.5, Blast_Score=210, Evalue=9e-56,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_CHLCH (Q3APK0)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380118.1
- ProteinModelPortal:   Q3APK0
- STRING:   Q3APK0
- GeneID:   3746455
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1824
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   CCHL340177:CAG_1824-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37100; Mature: 37100

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYQMQMPAKIELDESSHSDSFGKFIAQPLERGYGVTLGNLMRRVLLASLPGTAITGIKI
CEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECEEE
ENVYHEFSTIQGVREDVPEIVLNLKKVRFRSQCKRSCKTTVTLVGPMEFTAGVIQPQEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCC
FEVLNKDLHIATINAGTTVTLDIFIGRGRGYVPAEENRAEGMPLGFIPIDSIFTPIRNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCEE
FTVENTRVGQRTDYEKMILEVETDGSITPDDSISLAGRVISDHVLLFADFSPAEEEYTEE
EEEECCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHH
EFKQQDDEFETMRRLLATKIEDLDLSVRSHNCLRLAEIDTLGELVSHKEDELLNYKNFGK
HHHCCCHHHHHHHHHHHHHHHHCEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCH
KSLTELKEQLDKFDLKFGMDITRYQMK
HHHHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MIYQMQMPAKIELDESSHSDSFGKFIAQPLERGYGVTLGNLMRRVLLASLPGTAITGIKI
CEEEECCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECEEE
ENVYHEFSTIQGVREDVPEIVLNLKKVRFRSQCKRSCKTTVTLVGPMEFTAGVIQPQEGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCCC
FEVLNKDLHIATINAGTTVTLDIFIGRGRGYVPAEENRAEGMPLGFIPIDSIFTPIRNVK
CEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHCEE
FTVENTRVGQRTDYEKMILEVETDGSITPDDSISLAGRVISDHVLLFADFSPAEEEYTEE
EEEECCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHH
EFKQQDDEFETMRRLLATKIEDLDLSVRSHNCLRLAEIDTLGELVSHKEDELLNYKNFGK
HHHCCCHHHHHHHHHHHHHHHHCEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCH
KSLTELKEQLDKFDLKFGMDITRYQMK
HHHHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA