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The map label for this gene is ispF

Identifier: 78189738

GI number: 78189738

Start: 2300836

End: 2301309

Strand: Reverse

Name: ispF

Synonym: Cag_1782

Alternate gene names: 78189738

Gene position: 2301309-2300836 (Counterclockwise)

Preceding gene: 78189739

Following gene: 78189736

Centisome position: 89.47

GC content: 47.47

Gene sequence:

>474_bases
ATGCGAATTGGAATAGGAATTGATGTACACCCCTTTGCCGCTGGACGTAAATTAGTAATTGGCGGCGTCTATATTCCCGG
ACACGATGGTTTAGATGGGCATAGCGATGCCGATGTATTACTCCATGCCATTAGCGATGCACTGCTTGGCGCAGCCGCAC
TTGGCGATATTGGGCTTCACTTTCCCAACACAAGTGCAGAGTTTAAGGATATTGATAGCATGATTTTGCTCAAGCATGTA
GGAAAGTTGCTTGCAGAGCATGGTTATGCGCCAGTAAACGTTGATGCCATGCTTTTGCTTGAAGCGCCTAAAATTGCGCC
TTACATTCAGCAAATGCGTCAAAACATTGCAGATGCTCTTGGGCTTCAAGTCGGCGATGTTGCCGTAAAAGCCACCACCA
ACGAAAAGCTTGGCTACATTGGTCGCAAAGAGGGAGCTGAAGCACACGCGGTATGTTTGATTCAGGGGAAGTAA

Upstream 100 bases:

>100_bases
CGCCATGCCACTCCGCGATTATTGCGGAAGTTTACAGGTAGAATAAATTATTCAGAATAGGATAAATCCTTATTGAACAA
CTCTTTAACAGCTATTATAT

Downstream 100 bases:

>100_bases
GATAGGGGAGTGCTGCGTATGTGCAGTAGAGAAAAAAGAAGGTTCTTCTTATTCGCTACTGCACTTTTTTTGTTATTACA
CTAATTTTAAAACACCGATA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGIGIDVHPFAAGRKLVIGGVYIPGHDGLDGHSDADVLLHAISDALLGAAALGDIGLHFPNTSAEFKDIDSMILLKHV
GKLLAEHGYAPVNVDAMLLLEAPKIAPYIQQMRQNIADALGLQVGDVAVKATTNEKLGYIGRKEGAEAHAVCLIQGK

Sequences:

>Translated_157_residues
MRIGIGIDVHPFAAGRKLVIGGVYIPGHDGLDGHSDADVLLHAISDALLGAAALGDIGLHFPNTSAEFKDIDSMILLKHV
GKLLAEHGYAPVNVDAMLLLEAPKIAPYIQQMRQNIADALGLQVGDVAVKATTNEKLGYIGRKEGAEAHAVCLIQGK
>Mature_157_residues
MRIGIGIDVHPFAAGRKLVIGGVYIPGHDGLDGHSDADVLLHAISDALLGAAALGDIGLHFPNTSAEFKDIDSMILLKHV
GKLLAEHGYAPVNVDAMLLLEAPKIAPYIQQMRQNIADALGLQVGDVAVKATTNEKLGYIGRKEGAEAHAVCLIQGK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=53.2467532467532, Blast_Score=142, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLCH (Q3APP2)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_380076.1
- ProteinModelPortal:   Q3APP2
- SMR:   Q3APP2
- STRING:   Q3APP2
- GeneID:   3747202
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1782
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   CCHL340177:CAG_1782-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16504; Mature: 16504

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIGIDVHPFAAGRKLVIGGVYIPGHDGLDGHSDADVLLHAISDALLGAAALGDIGLH
CEEECCCEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FPNTSAEFKDIDSMILLKHVGKLLAEHGYAPVNVDAMLLLEAPKIAPYIQQMRQNIADAL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GLQVGDVAVKATTNEKLGYIGRKEGAEAHAVCLIQGK
CCEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRIGIGIDVHPFAAGRKLVIGGVYIPGHDGLDGHSDADVLLHAISDALLGAAALGDIGLH
CEEECCCEECCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FPNTSAEFKDIDSMILLKHVGKLLAEHGYAPVNVDAMLLLEAPKIAPYIQQMRQNIADAL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
GLQVGDVAVKATTNEKLGYIGRKEGAEAHAVCLIQGK
CCEECCEEEEEECCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA