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The map label for this gene is relA [H]

Identifier: 78189731

GI number: 78189731

Start: 2291651

End: 2293858

Strand: Reverse

Name: relA [H]

Synonym: Cag_1775

Alternate gene names: 78189731

Gene position: 2293858-2291651 (Counterclockwise)

Preceding gene: 78189733

Following gene: 78189730

Centisome position: 89.18

GC content: 44.79

Gene sequence:

>2208_bases
ATGATAGCCCAAATAGACAAAGAGCATTACCAAAAAATCCATGAGATTCTTGCCCTTGCGCGTCGGAACTTAAAAAATGT
TGATGAGTCGCTGATTCAGCGTGCCTTTTTTATGTGCTATCGGGCGCATGAAGGCGAAAAGCGTGCGTCGGGCGAACCCT
TTTTTTATCACCCTGTGGAGGTGGCAACTATTTTGCTTAACGAGCTGCCGCTTGATGGCGTGTCGGTAGCGGCGGCGTTG
CTGCACGATGTTATTGAGGATAGCGGTTACACGTATGACGATATTGTGGCTGAGCTTGGCGTTGAGGTTGCCGACATTGT
GGAGGGGCTTACCAAAATTTCGGGCATAACCATTAATCGTGAAACCACGCAAGCTGAAGGGTTCCGCAAAATGTTGCTTT
CAATGGTGAAGGATATTCGTGTGATCCTCATCAAGTTTTGCGACCGCTTGCACAACATGCGCACTTTGGAGTCGCTGCCA
GAGCATCGGCGCCTCAAAATTGCTTTAGAGACGCGCGATATATTTGCGCCTCTTGCCCACCGTTTTGGTCTCGGCAAAAT
GAAGGTGGAGTTTGAAAATTTAGCGCTTAAATACATTGATCCCGAAATGTATCGCTTTTTGGAGCAGAAGTTGCGCATGA
ATAAGGGCGACCGTGTAAGCTACCTGAGCAAAATGATTCAGCCCATTAAAAGCGATTTAGAGCAGCAAGGGTTTAAGGTA
GAAGTGCAAGGGCGAGCGAAGCATCTTTTTTCTATATACAATAAAATGCGCAATAAAAACAAGAGCTTTGAGGACATTCA
CGACCTCTATGGCATTCGCATTATTATTGATACCGAGCGCATTGCCGATTGCTTTTCGGTGTATGGATTTATTACACAGC
AATATCCACCACTGCCGCAATACTTTAAGGATTACATCTCCATTCCAAAACATAACGGCTACCAATCGCTCCATTCAGCC
ATTATTGGACCAAAAGGCAATATGGTTGAAATTCAAATTCGCACCCGACGAATGCACGAGTTTGCTGAGCTTGGTGTGGC
TGCCCATTGGCGTTACAAGGAAAAAATTTCGCAAGATGATGCTCATGTTGATTCTTTTTTGCGTTGGGCGCGTGAGCTTA
TTAAGGATGCTGATTCCGCAACGGAATTTATGGAGGGCTTTAAGCTCAATCTTTACCATGAAGAAATTTATGTGTTTACG
CCTAAGGGCGATATGAAAACACTACCTGCGGGAGCTACACCTATTGATTTTGCTTACGCCATTCATACCGAAGTTGGGCA
TGGCTGCATTGGTGCCAAGGTAAATGGCAAAATTGTGCGGCTTAATGCCATTTTAAAGTCGGGCGATAGGGTTGAAATTA
TTACCTCCAAAACCTCAAAGCCCAAAGCTGATTGGCTGAACATTGTGGTTACGCAGCGGGCACAAATGAAAATTCGCTCG
GCTATTAATGAGGATCGCCGCTTGCAAATTGAGAAGGGGCGAAATATTTGGGATAAGTTGGTGAGCAACACCAAAAAGCT
CTTAACCGATAATGAAATTGTTCAACAGGCGAAAAAATATGGCATTAAAACGCCGAGCGACTTTTTTAGCGCATTAGCAA
ATCAAGAAATTAATGGTGAAGAGGTTTTAGCGCAAGCAAGTGCGGCGAAAAAAGCGGAGCAGCTTACCAAAAGCGTTATG
GATGAGGCGCGTGAGCTTGATGAATATTTGCAAGCCGCACGCCAAGAGGCGGAACCTGAGCAGCAAAAAATAGCCAGCCG
TCGTGATGAAGTGGTTATTGCAGGTATGAGCAACATTGCTTATTCCTACGCAAAATGTTGCCAACCCGTGCCGGGCGACG
ACGTTATTGGTTTTGTGTCAACTGATGGCGTGGTGCGCATTCATCGCAAAAATTGTATTAACGTTAGCAACGACAGCCTT
GTAAAAAGCGAGCGAGTTGTTGCGGTAGCGTGGAATCGTAAAATTGATACGGAGTTTCTTGCAGGTATTCGCATTATTGG
CGAAGATCGGGTAGGCATTATGAACCAAATTACAACGGTGATTTCAAAGTTAGATGTAAACATTCGCACCATTTCGCTTA
ATGCCAACGATGGTATGTTTGTGGGCATTATGACTATTTTTGTGCGCAATGCCGATAAGCTTTCATCGTTAATGGAGAAA
CTTAAAAAGGTGCAAGGAGTGTTTACCGTAGAGCGTTTGATAAGCTAA

Upstream 100 bases:

>100_bases
TTTGCTTCTCCCGCAAAAGCTTTTTTGTATGTTCCTCTAAGAGCATTTTAAAAAAGCCATTCCCCCCTTTTCTTTTTCAT
TTATTTGATTACCTGAAGCC

Downstream 100 bases:

>100_bases
GAGGGGGGGCATTCCCGTGAAGACGCGCAATCCATTAAATTTTTCATTCATCACGAATCATGTACATTTATCATTTAAAT
CAACCACAACAAGGAGAACA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MIAQIDKEHYQKIHEILALARRNLKNVDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDIVAELGVEVADIVEGLTKISGITINRETTQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIFAPLAHRFGLGKMKVEFENLALKYIDPEMYRFLEQKLRMNKGDRVSYLSKMIQPIKSDLEQQGFKV
EVQGRAKHLFSIYNKMRNKNKSFEDIHDLYGIRIIIDTERIADCFSVYGFITQQYPPLPQYFKDYISIPKHNGYQSLHSA
IIGPKGNMVEIQIRTRRMHEFAELGVAAHWRYKEKISQDDAHVDSFLRWARELIKDADSATEFMEGFKLNLYHEEIYVFT
PKGDMKTLPAGATPIDFAYAIHTEVGHGCIGAKVNGKIVRLNAILKSGDRVEIITSKTSKPKADWLNIVVTQRAQMKIRS
AINEDRRLQIEKGRNIWDKLVSNTKKLLTDNEIVQQAKKYGIKTPSDFFSALANQEINGEEVLAQASAAKKAEQLTKSVM
DEARELDEYLQAARQEAEPEQQKIASRRDEVVIAGMSNIAYSYAKCCQPVPGDDVIGFVSTDGVVRIHRKNCINVSNDSL
VKSERVVAVAWNRKIDTEFLAGIRIIGEDRVGIMNQITTVISKLDVNIRTISLNANDGMFVGIMTIFVRNADKLSSLMEK
LKKVQGVFTVERLIS

Sequences:

>Translated_735_residues
MIAQIDKEHYQKIHEILALARRNLKNVDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDIVAELGVEVADIVEGLTKISGITINRETTQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIFAPLAHRFGLGKMKVEFENLALKYIDPEMYRFLEQKLRMNKGDRVSYLSKMIQPIKSDLEQQGFKV
EVQGRAKHLFSIYNKMRNKNKSFEDIHDLYGIRIIIDTERIADCFSVYGFITQQYPPLPQYFKDYISIPKHNGYQSLHSA
IIGPKGNMVEIQIRTRRMHEFAELGVAAHWRYKEKISQDDAHVDSFLRWARELIKDADSATEFMEGFKLNLYHEEIYVFT
PKGDMKTLPAGATPIDFAYAIHTEVGHGCIGAKVNGKIVRLNAILKSGDRVEIITSKTSKPKADWLNIVVTQRAQMKIRS
AINEDRRLQIEKGRNIWDKLVSNTKKLLTDNEIVQQAKKYGIKTPSDFFSALANQEINGEEVLAQASAAKKAEQLTKSVM
DEARELDEYLQAARQEAEPEQQKIASRRDEVVIAGMSNIAYSYAKCCQPVPGDDVIGFVSTDGVVRIHRKNCINVSNDSL
VKSERVVAVAWNRKIDTEFLAGIRIIGEDRVGIMNQITTVISKLDVNIRTISLNANDGMFVGIMTIFVRNADKLSSLMEK
LKKVQGVFTVERLIS
>Mature_735_residues
MIAQIDKEHYQKIHEILALARRNLKNVDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVEVATILLNELPLDGVSVAAAL
LHDVIEDSGYTYDDIVAELGVEVADIVEGLTKISGITINRETTQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLP
EHRRLKIALETRDIFAPLAHRFGLGKMKVEFENLALKYIDPEMYRFLEQKLRMNKGDRVSYLSKMIQPIKSDLEQQGFKV
EVQGRAKHLFSIYNKMRNKNKSFEDIHDLYGIRIIIDTERIADCFSVYGFITQQYPPLPQYFKDYISIPKHNGYQSLHSA
IIGPKGNMVEIQIRTRRMHEFAELGVAAHWRYKEKISQDDAHVDSFLRWARELIKDADSATEFMEGFKLNLYHEEIYVFT
PKGDMKTLPAGATPIDFAYAIHTEVGHGCIGAKVNGKIVRLNAILKSGDRVEIITSKTSKPKADWLNIVVTQRAQMKIRS
AINEDRRLQIEKGRNIWDKLVSNTKKLLTDNEIVQQAKKYGIKTPSDFFSALANQEINGEEVLAQASAAKKAEQLTKSVM
DEARELDEYLQAARQEAEPEQQKIASRRDEVVIAGMSNIAYSYAKCCQPVPGDDVIGFVSTDGVVRIHRKNCINVSNDSL
VKSERVVAVAWNRKIDTEFLAGIRIIGEDRVGIMNQITTVISKLDVNIRTISLNANDGMFVGIMTIFVRNADKLSSLMEK
LKKVQGVFTVERLIS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=38.048090523338, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1789147, Length=720, Percent_Identity=33.1944444444444, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83694; Mature: 83694

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQIDKEHYQKIHEILALARRNLKNVDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VATILLNELPLDGVSVAAALLHDVIEDSGYTYDDIVAELGVEVADIVEGLTKISGITINR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEECC
ETTQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLPEHRRLKIALETRDIFAPLAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHH
RFGLGKMKVEFENLALKYIDPEMYRFLEQKLRMNKGDRVSYLSKMIQPIKSDLEQQGFKV
HCCCCCEEEEHHHHHHEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
EVQGRAKHLFSIYNKMRNKNKSFEDIHDLYGIRIIIDTERIADCFSVYGFITQQYPPLPQ
EECHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
YFKDYISIPKHNGYQSLHSAIIGPKGNMVEIQIRTRRMHEFAELGVAAHWRYKEKISQDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AHVDSFLRWARELIKDADSATEFMEGFKLNLYHEEIYVFTPKGDMKTLPAGATPIDFAYA
HHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEVGHGCIGAKVNGKIVRLNAILKSGDRVEIITSKTSKPKADWLNIVVTQRAQMKIRS
EEHHCCCCEECCEECCEEEEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AINEDRRLQIEKGRNIWDKLVSNTKKLLTDNEIVQQAKKYGIKTPSDFFSALANQEINGE
HCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
EVLAQASAAKKAEQLTKSVMDEARELDEYLQAARQEAEPEQQKIASRRDEVVIAGMSNIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHH
YSYAKCCQPVPGDDVIGFVSTDGVVRIHRKNCINVSNDSLVKSERVVAVAWNRKIDTEFL
HHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHCCCEEEEEECCCCCHHHH
AGIRIIGEDRVGIMNQITTVISKLDVNIRTISLNANDGMFVGIMTIFVRNADKLSSLMEK
HCEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
LKKVQGVFTVERLIS
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAQIDKEHYQKIHEILALARRNLKNVDESLIQRAFFMCYRAHEGEKRASGEPFFYHPVE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECHHH
VATILLNELPLDGVSVAAALLHDVIEDSGYTYDDIVAELGVEVADIVEGLTKISGITINR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEECC
ETTQAEGFRKMLLSMVKDIRVILIKFCDRLHNMRTLESLPEHRRLKIALETRDIFAPLAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHH
RFGLGKMKVEFENLALKYIDPEMYRFLEQKLRMNKGDRVSYLSKMIQPIKSDLEQQGFKV
HCCCCCEEEEHHHHHHEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEE
EVQGRAKHLFSIYNKMRNKNKSFEDIHDLYGIRIIIDTERIADCFSVYGFITQQYPPLPQ
EECHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHCCCCCCHH
YFKDYISIPKHNGYQSLHSAIIGPKGNMVEIQIRTRRMHEFAELGVAAHWRYKEKISQDD
HHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCH
AHVDSFLRWARELIKDADSATEFMEGFKLNLYHEEIYVFTPKGDMKTLPAGATPIDFAYA
HHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHEE
IHTEVGHGCIGAKVNGKIVRLNAILKSGDRVEIITSKTSKPKADWLNIVVTQRAQMKIRS
EEHHCCCCEECCEECCEEEEEEHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AINEDRRLQIEKGRNIWDKLVSNTKKLLTDNEIVQQAKKYGIKTPSDFFSALANQEINGE
HCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCHH
EVLAQASAAKKAEQLTKSVMDEARELDEYLQAARQEAEPEQQKIASRRDEVVIAGMSNIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEHHHHH
YSYAKCCQPVPGDDVIGFVSTDGVVRIHRKNCINVSNDSLVKSERVVAVAWNRKIDTEFL
HHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHCCCEEEEEECCCCCHHHH
AGIRIIGEDRVGIMNQITTVISKLDVNIRTISLNANDGMFVGIMTIFVRNADKLSSLMEK
HCEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHH
LKKVQGVFTVERLIS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9383190; 9384377 [H]