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The map label for this gene is menE [H]

Identifier: 78189629

GI number: 78189629

Start: 2171327

End: 2172796

Strand: Reverse

Name: menE [H]

Synonym: Cag_1672

Alternate gene names: 78189629

Gene position: 2172796-2171327 (Counterclockwise)

Preceding gene: 78189634

Following gene: 78189628

Centisome position: 84.48

GC content: 48.5

Gene sequence:

>1470_bases
ATGGATATTCTTGCCCATGCGGCAGCGCGTTTTGGTAATGCACCAATGCTGTATTTGCCCAATAGAGTCCTCTCTTTTCA
CCAGTGCAATGAGCAGGCAGCGGCAATTGCTGCACGTTTGCAAAGCAAAGGCGTGCGTGCGGGGGCGATTGTGGCAATTC
TTTCGCCGAACACACCTGAGTTGGTGCTCTTGTTGCTTGCTTTGTTGAAAAGTGGAATAATTGCAGCTCCACTGAATCAT
CGGTTGCCGCAGCCGTTGCTTACGCGTATGGTTGAGCGCTTGCAGCCTTGCTTACTTATTTCCGACATTGATGCTCCTCA
TATCTCTAATATAAACAACCATCTTTCGTTCAGTAGCTTGCTTGACGGTATTACATACAGTAACACAAGCCAAAGCGGTT
GCCCATTATTGTCGGCTGAGAGCGAGGTTACCCGCAACAATTTCCCAGCCGATGCCATGCAGCAACCTGTAACCATTATT
CACACGTCGGCAAGTTCAGGTGAAGCAAAAGCTGCATTGCATAGTTTGGCAAACCATTGGTACAGTGCACTTGGCTCCAA
CCACAATCTTCCATTTGCTTCAGGCGATTGTTGGTTGCTCTCTCTGCCACTCTGCCATATTGGTGGCTATTCGCTCCTTT
TTCGTGCCTTGCTTTCTGGTGGCGCCCTTGCAATCAGCGCTCCCCATGCAAGCCTTAGCAATGCGCTGAGCAATTTTCCC
CTAACCCATCTCTCGTTAGTGCCAACACAACTCTATCGCTTACTGGCAGATAGTGAAAATGGTGAGCAGTTCCGTTCAAT
TAAAGCTATCTTATTGGGGGGAAGTGCTGCACCCGCATCGCTGATTGAGGAGGCATTGCGCCGCCAATTACCGCTCTACC
TAACCTATGGTTCAACGGAAATGAGCTCGCAAATTGCAACCTCTTTCAAGCCATTAACCACCTTGCAATTGGATAGCGGT
ACAGTGCTACCATATCGGCAGGTTGCCGTGTCGGATGATGGTGAGTTGCTCGTTAAAGGCGAATGTTTGTTTCTTGGCTA
TTTGCGAGATGGTGAGATTGCACCGCAACGCGATGGCGATGGTTGGTTTCACACGGCTGATGTTGGCACCCTTGCAGCGG
ATGGCACCCTTACGGTGTTGGGGAGAAAAGACAACATGTTTATTGTAGGGGGGGAAAATATTCATCCCGAAGAGATTGAG
CGAGCGCTTTTACAAATTGATAGCATTCATGAGGCAATTGTGGTACCTGCACCCGATGCCGAGTATGGTCAGCGCCCCGT
AGCCTTTATTGCCACCACTCACCTCAACGAGCCTACCGATGCGGCATTGATGCAGCAAATGCGCTTGTTTGTAGGAACGC
TTAAAACGCCACAACGCTTTTATCGAGTTACCGAGTGGGAATTGCTTTCTGGTTCACAAAAAATTAATCGTCGTTTTTAT
AAAGAATTCGTTTTACACAGTATTACTTGA

Upstream 100 bases:

>100_bases
TTCAACTAATTGTGTAATGTTTCAACGTGATTGTTAAAAGCAATTGCCATAAAGATAATAAAAGAAGCTCTCTCTACAAA
TCTTCACTGCAAGCTCATTT

Downstream 100 bases:

>100_bases
CCAACTGTTGTGTCATACTTTTTCTGTCATGTTTCACTTCGCTGCGGCAATGACAATAAAAAATCAATCATCCGCAATTC
TTGATCTATTATTCATCATT

Product: O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MDILAHAAARFGNAPMLYLPNRVLSFHQCNEQAAAIAARLQSKGVRAGAIVAILSPNTPELVLLLLALLKSGIIAAPLNH
RLPQPLLTRMVERLQPCLLISDIDAPHISNINNHLSFSSLLDGITYSNTSQSGCPLLSAESEVTRNNFPADAMQQPVTII
HTSASSGEAKAALHSLANHWYSALGSNHNLPFASGDCWLLSLPLCHIGGYSLLFRALLSGGALAISAPHASLSNALSNFP
LTHLSLVPTQLYRLLADSENGEQFRSIKAILLGGSAAPASLIEEALRRQLPLYLTYGSTEMSSQIATSFKPLTTLQLDSG
TVLPYRQVAVSDDGELLVKGECLFLGYLRDGEIAPQRDGDGWFHTADVGTLAADGTLTVLGRKDNMFIVGGENIHPEEIE
RALLQIDSIHEAIVVPAPDAEYGQRPVAFIATTHLNEPTDAALMQQMRLFVGTLKTPQRFYRVTEWELLSGSQKINRRFY
KEFVLHSIT

Sequences:

>Translated_489_residues
MDILAHAAARFGNAPMLYLPNRVLSFHQCNEQAAAIAARLQSKGVRAGAIVAILSPNTPELVLLLLALLKSGIIAAPLNH
RLPQPLLTRMVERLQPCLLISDIDAPHISNINNHLSFSSLLDGITYSNTSQSGCPLLSAESEVTRNNFPADAMQQPVTII
HTSASSGEAKAALHSLANHWYSALGSNHNLPFASGDCWLLSLPLCHIGGYSLLFRALLSGGALAISAPHASLSNALSNFP
LTHLSLVPTQLYRLLADSENGEQFRSIKAILLGGSAAPASLIEEALRRQLPLYLTYGSTEMSSQIATSFKPLTTLQLDSG
TVLPYRQVAVSDDGELLVKGECLFLGYLRDGEIAPQRDGDGWFHTADVGTLAADGTLTVLGRKDNMFIVGGENIHPEEIE
RALLQIDSIHEAIVVPAPDAEYGQRPVAFIATTHLNEPTDAALMQQMRLFVGTLKTPQRFYRVTEWELLSGSQKINRRFY
KEFVLHSIT
>Mature_489_residues
MDILAHAAARFGNAPMLYLPNRVLSFHQCNEQAAAIAARLQSKGVRAGAIVAILSPNTPELVLLLLALLKSGIIAAPLNH
RLPQPLLTRMVERLQPCLLISDIDAPHISNINNHLSFSSLLDGITYSNTSQSGCPLLSAESEVTRNNFPADAMQQPVTII
HTSASSGEAKAALHSLANHWYSALGSNHNLPFASGDCWLLSLPLCHIGGYSLLFRALLSGGALAISAPHASLSNALSNFP
LTHLSLVPTQLYRLLADSENGEQFRSIKAILLGGSAAPASLIEEALRRQLPLYLTYGSTEMSSQIATSFKPLTTLQLDSG
TVLPYRQVAVSDDGELLVKGECLFLGYLRDGEIAPQRDGDGWFHTADVGTLAADGTLTVLGRKDNMFIVGGENIHPEEIE
RALLQIDSIHEAIVVPAPDAEYGQRPVAFIATTHLNEPTDAALMQQMRLFVGTLKTPQRFYRVTEWELLSGSQKINRRFY
KEFVLHSIT

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=471, Percent_Identity=25.0530785562633, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI122937307, Length=424, Percent_Identity=25.9433962264151, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI156151445, Length=519, Percent_Identity=24.8554913294798, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI115511026, Length=456, Percent_Identity=23.9035087719298, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI42794760, Length=495, Percent_Identity=23.6363636363636, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI42794758, Length=495, Percent_Identity=23.6363636363636, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI42794756, Length=495, Percent_Identity=23.6363636363636, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI42544132, Length=474, Percent_Identity=23.2067510548523, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40807491, Length=517, Percent_Identity=21.4700193423598, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788595, Length=474, Percent_Identity=31.6455696202532, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI145693145, Length=498, Percent_Identity=23.6947791164659, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788107, Length=485, Percent_Identity=22.4742268041237, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1786801, Length=452, Percent_Identity=24.3362831858407, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786810, Length=517, Percent_Identity=23.5976789168279, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=486, Percent_Identity=27.3662551440329, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=480, Percent_Identity=24.7916666666667, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=506, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=469, Percent_Identity=24.9466950959488, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=467, Percent_Identity=21.8415417558887, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=467, Percent_Identity=21.8415417558887, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=467, Percent_Identity=21.8415417558887, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=332, Percent_Identity=23.1927710843373, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=502, Percent_Identity=21.5139442231076, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538037, Length=276, Percent_Identity=26.4492753623188, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=357, Percent_Identity=25.4901960784314, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581924, Length=496, Percent_Identity=25.8064516129032, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922652, Length=432, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21355181, Length=456, Percent_Identity=23.2456140350877, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20130357, Length=271, Percent_Identity=25.4612546125461, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356947, Length=434, Percent_Identity=22.3502304147465, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53048; Mature: 53048

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILAHAAARFGNAPMLYLPNRVLSFHQCNEQAAAIAARLQSKGVRAGAIVAILSPNTPE
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
LVLLLLALLKSGIIAAPLNHRLPQPLLTRMVERLQPCLLISDIDAPHISNINNHLSFSSL
HHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHCCCHHHH
LDGITYSNTSQSGCPLLSAESEVTRNNFPADAMQQPVTIIHTSASSGEAKAALHSLANHW
HCCCEECCCCCCCCCEECCCCHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
YSALGSNHNLPFASGDCWLLSLPLCHIGGYSLLFRALLSGGALAISAPHASLSNALSNFP
HHHHCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCC
LTHLSLVPTQLYRLLADSENGEQFRSIKAILLGGSAAPASLIEEALRRQLPLYLTYGSTE
CHHHHHHHHHHHHHHHCCCCCHHHHHEEHHEECCCCCCHHHHHHHHHCCCCEEEEECCHH
MSSQIATSFKPLTTLQLDSGTVLPYRQVAVSDDGELLVKGECLFLGYLRDGEIAPQRDGD
HHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCEEEEEEEECCCCCCCCCCC
GWFHTADVGTLAADGTLTVLGRKDNMFIVGGENIHPEEIERALLQIDSIHEAIVVPAPDA
CCEEECCCCCEECCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCH
EYGQRPVAFIATTHLNEPTDAALMQQMRLFVGTLKTPQRFYRVTEWELLSGSQKINRRFY
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCHHHHHHHHH
KEFVLHSIT
HHHHHHCCC
>Mature Secondary Structure
MDILAHAAARFGNAPMLYLPNRVLSFHQCNEQAAAIAARLQSKGVRAGAIVAILSPNTPE
CCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHH
LVLLLLALLKSGIIAAPLNHRLPQPLLTRMVERLQPCLLISDIDAPHISNINNHLSFSSL
HHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCHHCCCHHHH
LDGITYSNTSQSGCPLLSAESEVTRNNFPADAMQQPVTIIHTSASSGEAKAALHSLANHW
HCCCEECCCCCCCCCEECCCCHHHCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHHHHHH
YSALGSNHNLPFASGDCWLLSLPLCHIGGYSLLFRALLSGGALAISAPHASLSNALSNFP
HHHHCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHHHHCCCEEEEECCCHHHHHHHHCCC
LTHLSLVPTQLYRLLADSENGEQFRSIKAILLGGSAAPASLIEEALRRQLPLYLTYGSTE
CHHHHHHHHHHHHHHHCCCCCHHHHHEEHHEECCCCCCHHHHHHHHHCCCCEEEEECCHH
MSSQIATSFKPLTTLQLDSGTVLPYRQVAVSDDGELLVKGECLFLGYLRDGEIAPQRDGD
HHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCEEEEEEEECCCCCCCCCCC
GWFHTADVGTLAADGTLTVLGRKDNMFIVGGENIHPEEIERALLQIDSIHEAIVVPAPDA
CCEEECCCCCEECCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCH
EYGQRPVAFIATTHLNEPTDAALMQQMRLFVGTLKTPQRFYRVTEWELLSGSQKINRRFY
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHCCHHHHHHHHH
KEFVLHSIT
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]