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The map label for this gene is kefB [H]

Identifier: 78189510

GI number: 78189510

Start: 2031558

End: 2033306

Strand: Reverse

Name: kefB [H]

Synonym: Cag_1550

Alternate gene names: 78189510

Gene position: 2033306-2031558 (Counterclockwise)

Preceding gene: 78189514

Following gene: 78189505

Centisome position: 79.05

GC content: 44.71

Gene sequence:

>1749_bases
ATGCACGACTTCTCGTTTCTTGGTGAGTTAGTATTAATTGGCGCTGCTGCCATAGCAATTATTCTGATTTTTCAGCGGCT
CAAAATCCCGTCAGTTATCGGGTTAATTTTTACTGGCATTCTTATTGGACCCTCAGGCATTGGTGCGGTTTATGATCAAA
AAATGATTTCCACATTAGCTGAGCTTGGTGTGGTGCTGTTGCTTTTTACCATTGGGCTTGAGTTTTCGCTTGAGGAGTTA
AAAAAGCTGAGGAAGGTGGTGTTGGTTGGTGGTGTGGCACAAATTATTGCCACAACCTTGGTTATTAGTGCCCTTGCCTC
TTGGCTTATGCCCATAATGGGGGAGCCAATTAGTGTGCCAACGGCGTTTTTTCTTGGTTCTACTTTTTCGGTAAGTAGTA
CGGCAATTTGTTTAAAAATTTTAGCGGAGCGTGAAGAGCTGTCGCTGCCGTATGGGCGCATTGCACTTGGCATTTTAATT
TTTCAAGATCTTGCCATTGTGCCATTAATGTTGGGCATTAACATGATTGCGCCCGGTGCCTCCTCTTCGTTTTATGCTAT
GATGAAGGAAATTGGCTTGGTGATTGTGTTGGCAGTTGCCATTGTGCTTGGTTTTCGTTTACTGATGCCCAAACTTGTGC
GCTTTATTGTTTCCCTTAAGGCGCGCGAAGTGTTGGTACTTGGTGCGCTTGTTATTTGCTTTGGAGCGGCTTACCTCACC
TCATTGGCAGGGCTTTCGTTAGCGCTTGGCTCTTTTGTGGCTGGTATGGTGATTGCAAGTACCGATGAGAGTCATCAAAT
TAGTGTGATTATTGATCCATTTCGCGAAGCCTTTAGCAGCCTCTTTTTTATTTCGGTGGGATTGTTGCTGGATGTAAACA
TGGAGAATCTGCCGCTTTTTATAGCCATTGCCGTTGTTGTGTTGTTGGTGAAAGGGTTGGTGGTGGCTGGTATTGGGCTG
TTTCTTGGCAATTCGCTTCGTGTAAGCATGATGGCTGGTATGGCGCTTGCTCAAATTGGAGAATTCTCTTTTGTCTTAGC
CGAAACAGGCTTACACAATGGGCTTATTAGTCGCGATATTTTTCAAGCCATGTTGGTCATTATTGTGGTAACCATGATTG
TTACCCCAGCAATGATTGCCGTTGCACCAAAAGTTGCCGATCAAGTGGTGCCTGCTTTTGGCTTTATTCCACTTGTAGGG
CGCTATACCGCTCCACCAGCACCAACGCAAGCCGTTCGTCCACCCAACAGCGCTATTATTTGTGCGGGCGAGATTCATGC
GGCAATTATTGGCTTTGGGGTGAATGGGCAAAATGTGGCAGCCGTACTTCATGCAACCAACATCTCCTATTCAGCGCTTG
AAATAGATCGTTACATTGTAAAAACCATGCGCCGTAAAGGCGAGCCAATTTTTTATGGCGATTGCACCGAAAAAAAGTCG
CTTTTGCGAGCTGGTATTGACCATGCACGAGCGGTGGTGATTGGTATTTCTGATCATACGGCTATTAGCAAAAGTATTGC
GTTAATCCGTGAGTTAAATGAGAAAGCCTACATTGTTGTACGAGCACGAACGTTAGACACGGTGGGTGATTTTTACCGTG
CAGGTGCTGATGTGGTGGTTACTGAAAAATTTGAAACCTCCATCCAAATTTTTTCTCAGTTGCTAAACCATTTTACCGTT
GCCCCCGATCTTATTTTAGAGCAGCAAGAAATTATTCGCCGTGAGTGCGAAAAGATTTTTTTAAAGTAG

Upstream 100 bases:

>100_bases
TACAATAGTTTGGCTTATTTGTAAAGCGCTTGCGTTGCTTAATGGCATAGGGTAAGTTCGCAAGTGCTGTTGAATAACGG
TTCTCTTTTTTACTCTTTTT

Downstream 100 bases:

>100_bases
AAGTTGCTTTTTGATCATCCCTGATGTTGTTTAGGCATTTTGGGCTGCTGTTACAAAGGCTCGCATTTTTGCAACATCTT
TAATGCCCGGTGCTTTTTCT

Product: glutathione-regulated potassium-efflux system protein KefC

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MHDFSFLGELVLIGAAAIAIILIFQRLKIPSVIGLIFTGILIGPSGIGAVYDQKMISTLAELGVVLLLFTIGLEFSLEEL
KKLRKVVLVGGVAQIIATTLVISALASWLMPIMGEPISVPTAFFLGSTFSVSSTAICLKILAEREELSLPYGRIALGILI
FQDLAIVPLMLGINMIAPGASSSFYAMMKEIGLVIVLAVAIVLGFRLLMPKLVRFIVSLKAREVLVLGALVICFGAAYLT
SLAGLSLALGSFVAGMVIASTDESHQISVIIDPFREAFSSLFFISVGLLLDVNMENLPLFIAIAVVVLLVKGLVVAGIGL
FLGNSLRVSMMAGMALAQIGEFSFVLAETGLHNGLISRDIFQAMLVIIVVTMIVTPAMIAVAPKVADQVVPAFGFIPLVG
RYTAPPAPTQAVRPPNSAIICAGEIHAAIIGFGVNGQNVAAVLHATNISYSALEIDRYIVKTMRRKGEPIFYGDCTEKKS
LLRAGIDHARAVVIGISDHTAISKSIALIRELNEKAYIVVRARTLDTVGDFYRAGADVVVTEKFETSIQIFSQLLNHFTV
APDLILEQQEIIRRECEKIFLK

Sequences:

>Translated_582_residues
MHDFSFLGELVLIGAAAIAIILIFQRLKIPSVIGLIFTGILIGPSGIGAVYDQKMISTLAELGVVLLLFTIGLEFSLEEL
KKLRKVVLVGGVAQIIATTLVISALASWLMPIMGEPISVPTAFFLGSTFSVSSTAICLKILAEREELSLPYGRIALGILI
FQDLAIVPLMLGINMIAPGASSSFYAMMKEIGLVIVLAVAIVLGFRLLMPKLVRFIVSLKAREVLVLGALVICFGAAYLT
SLAGLSLALGSFVAGMVIASTDESHQISVIIDPFREAFSSLFFISVGLLLDVNMENLPLFIAIAVVVLLVKGLVVAGIGL
FLGNSLRVSMMAGMALAQIGEFSFVLAETGLHNGLISRDIFQAMLVIIVVTMIVTPAMIAVAPKVADQVVPAFGFIPLVG
RYTAPPAPTQAVRPPNSAIICAGEIHAAIIGFGVNGQNVAAVLHATNISYSALEIDRYIVKTMRRKGEPIFYGDCTEKKS
LLRAGIDHARAVVIGISDHTAISKSIALIRELNEKAYIVVRARTLDTVGDFYRAGADVVVTEKFETSIQIFSQLLNHFTV
APDLILEQQEIIRRECEKIFLK
>Mature_582_residues
MHDFSFLGELVLIGAAAIAIILIFQRLKIPSVIGLIFTGILIGPSGIGAVYDQKMISTLAELGVVLLLFTIGLEFSLEEL
KKLRKVVLVGGVAQIIATTLVISALASWLMPIMGEPISVPTAFFLGSTFSVSSTAICLKILAEREELSLPYGRIALGILI
FQDLAIVPLMLGINMIAPGASSSFYAMMKEIGLVIVLAVAIVLGFRLLMPKLVRFIVSLKAREVLVLGALVICFGAAYLT
SLAGLSLALGSFVAGMVIASTDESHQISVIIDPFREAFSSLFFISVGLLLDVNMENLPLFIAIAVVVLLVKGLVVAGIGL
FLGNSLRVSMMAGMALAQIGEFSFVLAETGLHNGLISRDIFQAMLVIIVVTMIVTPAMIAVAPKVADQVVPAFGFIPLVG
RYTAPPAPTQAVRPPNSAIICAGEIHAAIIGFGVNGQNVAAVLHATNISYSALEIDRYIVKTMRRKGEPIFYGDCTEKKS
LLRAGIDHARAVVIGISDHTAISKSIALIRELNEKAYIVVRARTLDTVGDFYRAGADVVVTEKFETSIQIFSQLLNHFTV
APDLILEQQEIIRRECEKIFLK

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=393, Percent_Identity=27.4809160305344, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1789749, Length=579, Percent_Identity=28.4974093264249, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786232, Length=583, Percent_Identity=28.3018867924528, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1786685, Length=549, Percent_Identity=28.4153005464481, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322367, Length=416, Percent_Identity=24.2788461538462, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 62379; Mature: 62379

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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EHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA