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The map label for this gene is truB

Identifier: 78189421

GI number: 78189421

Start: 1925459

End: 1926226

Strand: Reverse

Name: truB

Synonym: Cag_1460

Alternate gene names: 78189421

Gene position: 1926226-1925459 (Counterclockwise)

Preceding gene: 78189422

Following gene: 78189420

Centisome position: 74.89

GC content: 50.39

Gene sequence:

>768_bases
ATGTGTAACGTTCACGCACAACAGGGGAGTGCGCAGGAAGCGCCACCGCCCGGAGAGTTGCTGCTGATTGATAAGCCGCT
TGATTGGACGTCGTTTGATGTGGTGGCAAAAGTGCGTAACACCTACCGCAAATGTGGCTCTAAGCGTAAAGTGGGGCATT
GTGGTACGCTTGATCCTAAAGCTACGGGTTTGCTTATTGTTGCCACAGGGCGCAAAACCAAAGAAATTTCACAGCTTGAG
CAACTCGATAAAGTATATGATGGTGTTATCAAGCTGGGAGCCATCACTGCCAGCCACGATACCGAAACACCTGAAGAGCA
GTTGTGTGATGTAGCGCATTTGAGCGAAGCCGAACTTCATGCCGTTGCAGCAACCTTTATTGGCAATCGTTTGCAGCAGC
CACCCATGCACTCCGCCGCTTGGCACAATGGGCGTCGCTTATATGAACATGCCCGCAAAGGTGAGGTTATTCGTGAACGT
AAAGCGCGCGAAATTGTGGTGCATCGCTTTACCATAACCAACGTTGCATTGCCGTTTGTTTCGTTTGAGCTGCATGTTTC
CAAAGGAGCCTACATTCGCGTTATAGCCGATGAGTTTGGCGCAGCGTTGGGCGTTGGCGGCTATCTTGCGGCGCTTCGCC
GTACAGCAATTGGGGAGTGGCAGTTAACCAATGCGCTTTCAGTAAACGATACTATTGAGCAGATTCATAACAACGCCTCA
AAAGGTGCTTTGTGTGGCACTGCCTCCACTATACATCCTGCAACATAA

Upstream 100 bases:

>100_bases
CGTTGATGATGCTGAAACGCCGCTTGACTAATGCTTGTTGTTACTGTTTCAGAGTTATCACTTCCCTTTTCCTTTATTAA
AAAAGTAAGGGGGAACTGTT

Downstream 100 bases:

>100_bases
AGAGCGCTCCCTATCATTCTTGAAAATATACGGGCGGCTCTCTTTTCTAAGTATAAATCTCATTCATGCGTTTAATCACC
TACGATAATCACCACGTTTA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCNVHAQQGSAQEAPPPGELLLIDKPLDWTSFDVVAKVRNTYRKCGSKRKVGHCGTLDPKATGLLIVATGRKTKEISQLE
QLDKVYDGVIKLGAITASHDTETPEEQLCDVAHLSEAELHAVAATFIGNRLQQPPMHSAAWHNGRRLYEHARKGEVIRER
KAREIVVHRFTITNVALPFVSFELHVSKGAYIRVIADEFGAALGVGGYLAALRRTAIGEWQLTNALSVNDTIEQIHNNAS
KGALCGTASTIHPAT

Sequences:

>Translated_255_residues
MCNVHAQQGSAQEAPPPGELLLIDKPLDWTSFDVVAKVRNTYRKCGSKRKVGHCGTLDPKATGLLIVATGRKTKEISQLE
QLDKVYDGVIKLGAITASHDTETPEEQLCDVAHLSEAELHAVAATFIGNRLQQPPMHSAAWHNGRRLYEHARKGEVIRER
KAREIVVHRFTITNVALPFVSFELHVSKGAYIRVIADEFGAALGVGGYLAALRRTAIGEWQLTNALSVNDTIEQIHNNAS
KGALCGTASTIHPAT
>Mature_255_residues
MCNVHAQQGSAQEAPPPGELLLIDKPLDWTSFDVVAKVRNTYRKCGSKRKVGHCGTLDPKATGLLIVATGRKTKEISQLE
QLDKVYDGVIKLGAITASHDTETPEEQLCDVAHLSEAELHAVAATFIGNRLQQPPMHSAAWHNGRRLYEHARKGEVIRER
KAREIVVHRFTITNVALPFVSFELHVSKGAYIRVIADEFGAALGVGGYLAALRRTAIGEWQLTNALSVNDTIEQIHNNAS
KGALCGTASTIHPAT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=175, Percent_Identity=30.2857142857143, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI2367200, Length=212, Percent_Identity=36.3207547169811, Blast_Score=117, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323204, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CHLCH (Q3AQK9)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379759.1
- ProteinModelPortal:   Q3AQK9
- SMR:   Q3AQK9
- STRING:   Q3AQK9
- GeneID:   3746429
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1460
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LNKVEKC
- ProtClustDB:   CLSK2516695
- BioCyc:   CCHL340177:CAG_1460-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 27784; Mature: 27784

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNVHAQQGSAQEAPPPGELLLIDKPLDWTSFDVVAKVRNTYRKCGSKRKVGHCGTLDPK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ATGLLIVATGRKTKEISQLEQLDKVYDGVIKLGAITASHDTETPEEQLCDVAHLSEAELH
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCHHHHH
AVAATFIGNRLQQPPMHSAAWHNGRRLYEHARKGEVIRERKAREIVVHRFTITNVALPFV
HHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHE
SFELHVSKGAYIRVIADEFGAALGVGGYLAALRRTAIGEWQLTNALSVNDTIEQIHNNAS
EEEEEECCCCEEEEEEHHHCCHHCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCC
KGALCGTASTIHPAT
CCCEECCCCCCCCCC
>Mature Secondary Structure
MCNVHAQQGSAQEAPPPGELLLIDKPLDWTSFDVVAKVRNTYRKCGSKRKVGHCGTLDPK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
ATGLLIVATGRKTKEISQLEQLDKVYDGVIKLGAITASHDTETPEEQLCDVAHLSEAELH
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHCCHHHHH
AVAATFIGNRLQQPPMHSAAWHNGRRLYEHARKGEVIRERKAREIVVHRFTITNVALPFV
HHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHE
SFELHVSKGAYIRVIADEFGAALGVGGYLAALRRTAIGEWQLTNALSVNDTIEQIHNNAS
EEEEEECCCCEEEEEEHHHCCHHCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCC
KGALCGTASTIHPAT
CCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA