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The map label for this gene is oprF [H]

Identifier: 78189221

GI number: 78189221

Start: 1720458

End: 1721726

Strand: Reverse

Name: oprF [H]

Synonym: Cag_1255

Alternate gene names: 78189221

Gene position: 1721726-1720458 (Counterclockwise)

Preceding gene: 78189222

Following gene: 78189220

Centisome position: 66.94

GC content: 33.57

Gene sequence:

>1269_bases
ATGACAATTAATCATTTTACTATGAAAAAGAATTTATTGACGTTATCACTATTGCTGAGTGCAACTGTTCCAGCAACAGC
CCAAATCAACTTAAAAAGCATTTTTGATAGTAGCGCTAAAAAAGCAGAAAGAAATGCTGCACAGCGCATAGAAAAAAAGA
TAGACAAAAAAGTTAATAATACTTTTGATAGTGTTGAAAATAATCTTGATAACGTAAAAAACAATTCTGAAAATAATAAC
TACGCATTTCAGGAACCAGTAAGCAATAGCATACCAGTTAAGCAACAATCTACACTTTCATGGAATAAATACGATTTTGT
TCCTGGTACAGAAATCATTTTTGAAGATGATTTTACCGGTGAAAGAAATGGTGAATTTCCTTCACGCTGGGATATTACCA
AAGGTACTGTTGAAATAGCAGAATGGGGTGGAGAAAAAGTGGTATGGTTTAAAAATACTAATACTAATGTACCTGATGCA
ATATTACCCTATCTTAAAAACAGATCAACAGACTATTTGCCCGATGAATTTACCTTTGAAATGGATGTTTATTTTCATGC
AGATTATAGGCTAAACAAAGATTATTATATTTTTTTCTATGATGCAAAAAATCAAACAAAGATATTTTCGCCATCCAAAC
CTATTCGAATAGATTATAATAGCGTTACGTATAATCATATAGGAGATCTTTATCAAGGGCAGAACAAATTGAAACCAAAA
GAAGGGTGGAGACATGTTGCAATTTCTTTTAACAAAAGAGCGTTAAAAGGATATTTGGATGATGCCCGTTTGTTGAATAT
TCCTAATGTTGAATTTAATCCTACAGGCATTATGATTTCTTCGCATAATTCTGGTGGCAAAGGTCAGCCTTTTGTGAAGA
ATATTCGTATTGCTAAGGGGGCTGTTCCTTTATATGATAAATTTTTGGTTGATGGGAAGTTTGTTACTACCGGAATCACA
TTTGATATAAACAAAGCAACCATAAAACCAGAAAGTATGGGAACCATAAACTATGTGGTTAAAATGATGCAAGAGCATCC
CGAACTGAAATTTAGTGTTGAAGGACACACTGATAGTGATGGCGCAGATGCCAATAATCAAACCCTTTCTGAAGCCAGAG
CTCAAGCAATAGTCAATAAGCTCATAGAATCAGGAATAGCTAAAGAAAGGCTTACCTCGAAAGGTTGGGGTGAAAGCAAA
CCAATAACAAATAACGAAACGGCTGAAGGTAAAGCGCAAAATAGAAGAGTAGAATTTATTAAAATTTAA

Upstream 100 bases:

>100_bases
GAAATGTTAAAGAACCGTTGTTGATAACGGTTCTTTAACCAAGAACGGTTTTAGGGAGTTCTGATAGTTTTACCCAAAAA
CCGAGTTGTGAATTTTTTAT

Downstream 100 bases:

>100_bases
CCCCTATGAAAAAATGAAAGGTAAACCAACATTCGTTATTGCTTTGGCATCGTGTTTGATTGTTTCGTGTACCAACAATA
CTCCGAAAACAGATGCTGAT

Product: peptidoglycan-associated (lipo)protein-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTINHFTMKKNLLTLSLLLSATVPATAQINLKSIFDSSAKKAERNAAQRIEKKIDKKVNNTFDSVENNLDNVKNNSENNN
YAFQEPVSNSIPVKQQSTLSWNKYDFVPGTEIIFEDDFTGERNGEFPSRWDITKGTVEIAEWGGEKVVWFKNTNTNVPDA
ILPYLKNRSTDYLPDEFTFEMDVYFHADYRLNKDYYIFFYDAKNQTKIFSPSKPIRIDYNSVTYNHIGDLYQGQNKLKPK
EGWRHVAISFNKRALKGYLDDARLLNIPNVEFNPTGIMISSHNSGGKGQPFVKNIRIAKGAVPLYDKFLVDGKFVTTGIT
FDINKATIKPESMGTINYVVKMMQEHPELKFSVEGHTDSDGADANNQTLSEARAQAIVNKLIESGIAKERLTSKGWGESK
PITNNETAEGKAQNRRVEFIKI

Sequences:

>Translated_422_residues
MTINHFTMKKNLLTLSLLLSATVPATAQINLKSIFDSSAKKAERNAAQRIEKKIDKKVNNTFDSVENNLDNVKNNSENNN
YAFQEPVSNSIPVKQQSTLSWNKYDFVPGTEIIFEDDFTGERNGEFPSRWDITKGTVEIAEWGGEKVVWFKNTNTNVPDA
ILPYLKNRSTDYLPDEFTFEMDVYFHADYRLNKDYYIFFYDAKNQTKIFSPSKPIRIDYNSVTYNHIGDLYQGQNKLKPK
EGWRHVAISFNKRALKGYLDDARLLNIPNVEFNPTGIMISSHNSGGKGQPFVKNIRIAKGAVPLYDKFLVDGKFVTTGIT
FDINKATIKPESMGTINYVVKMMQEHPELKFSVEGHTDSDGADANNQTLSEARAQAIVNKLIESGIAKERLTSKGWGESK
PITNNETAEGKAQNRRVEFIKI
>Mature_421_residues
TINHFTMKKNLLTLSLLLSATVPATAQINLKSIFDSSAKKAERNAAQRIEKKIDKKVNNTFDSVENNLDNVKNNSENNNY
AFQEPVSNSIPVKQQSTLSWNKYDFVPGTEIIFEDDFTGERNGEFPSRWDITKGTVEIAEWGGEKVVWFKNTNTNVPDAI
LPYLKNRSTDYLPDEFTFEMDVYFHADYRLNKDYYIFFYDAKNQTKIFSPSKPIRIDYNSVTYNHIGDLYQGQNKLKPKE
GWRHVAISFNKRALKGYLDDARLLNIPNVEFNPTGIMISSHNSGGKGQPFVKNIRIAKGAVPLYDKFLVDGKFVTTGITF
DINKATIKPESMGTINYVVKMMQEHPELKFSVEGHTDSDGADANNQTLSEARAQAIVNKLIESGIAKERLTSKGWGESKP
ITNNETAEGKAQNRRVEFIKI

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=101, Percent_Identity=42.5742574257426, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787191, Length=112, Percent_Identity=36.6071428571429, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 47886; Mature: 47755

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINHFTMKKNLLTLSLLLSATVPATAQINLKSIFDSSAKKAERNAAQRIEKKIDKKVNN
CCCCCEEHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
TFDSVENNLDNVKNNSENNNYAFQEPVSNSIPVKQQSTLSWNKYDFVPGTEIIFEDDFTG
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCC
ERNGEFPSRWDITKGTVEIAEWGGEKVVWFKNTNTNVPDAILPYLKNRSTDYLPDEFTFE
CCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEE
MDVYFHADYRLNKDYYIFFYDAKNQTKIFSPSKPIRIDYNSVTYNHIGDLYQGQNKLKPK
EEEEEEECEEECCCEEEEEEECCCCEEEECCCCCEEEEECCEEHHHHHHHHCCCCCCCCC
EGWRHVAISFNKRALKGYLDDARLLNIPNVEFNPTGIMISSHNSGGKGQPFVKNIRIAKG
CCCEEEEEEECHHHHHHHCCCCEEEECCCCEECCCEEEEECCCCCCCCCCHHHHEEEECC
AVPLYDKFLVDGKFVTTGITFDINKATIKPESMGTINYVVKMMQEHPELKFSVEGHTDSD
CCCHHHHHEECCEEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
GADANNQTLSEARAQAIVNKLIESGIAKERLTSKGWGESKPITNNETAEGKAQNRRVEFI
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KI
EC
>Mature Secondary Structure 
TINHFTMKKNLLTLSLLLSATVPATAQINLKSIFDSSAKKAERNAAQRIEKKIDKKVNN
CCCCEEHHHHHHHHHHHHHCCCCCEEEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
TFDSVENNLDNVKNNSENNNYAFQEPVSNSIPVKQQSTLSWNKYDFVPGTEIIFEDDFTG
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCC
ERNGEFPSRWDITKGTVEIAEWGGEKVVWFKNTNTNVPDAILPYLKNRSTDYLPDEFTFE
CCCCCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEE
MDVYFHADYRLNKDYYIFFYDAKNQTKIFSPSKPIRIDYNSVTYNHIGDLYQGQNKLKPK
EEEEEEECEEECCCEEEEEEECCCCEEEECCCCCEEEEECCEEHHHHHHHHCCCCCCCCC
EGWRHVAISFNKRALKGYLDDARLLNIPNVEFNPTGIMISSHNSGGKGQPFVKNIRIAKG
CCCEEEEEEECHHHHHHHCCCCEEEECCCCEECCCEEEEECCCCCCCCCCHHHHEEEECC
AVPLYDKFLVDGKFVTTGITFDINKATIKPESMGTINYVVKMMQEHPELKFSVEGHTDSD
CCCHHHHHEECCEEEEEEEEEEECCEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCC
GADANNQTLSEARAQAIVNKLIESGIAKERLTSKGWGESKPITNNETAEGKAQNRRVEFI
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
KI
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]