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The map label for this gene is waaA [H]

Identifier: 78189082

GI number: 78189082

Start: 1503996

End: 1505306

Strand: Reverse

Name: waaA [H]

Synonym: Cag_1115

Alternate gene names: 78189082

Gene position: 1505306-1503996 (Counterclockwise)

Preceding gene: 78189085

Following gene: 78189081

Centisome position: 58.52

GC content: 49.73

Gene sequence:

>1311_bases
ATGCCCATTAGTTGCCACCACATGCAATCATTTCCCCTCTATCAAACCCTTTTTCCTTTACTTGCTGGCGTAGCGCAAGG
AGTAAAAGCACTTCATCCCCAAATAGCAGCATTTTTTGAGGTGCGTCAACATCTCTTTACAACGCTTCAGCAACAGTTGG
CAACCATGCCGAACAATGGTTTTCGGCTCTGGGTTCATGCAGCATCGGTAGGTGAATTTGAGCAAGCGCGTCCCATTATT
GCAGCCTTGCAAGCAAGGCATCCCAACTTACGGCTCTTTATTTCATTTCTTTCACCTTCGGGATATAATGCTCGCAAAAA
CTTTCCAAATGCCGCTGCTGTTTTTTACCTCCCGCTTGATACTGCCGCAAATGCTCGCAAGCTCGTGGCGCTTTTGAAGC
CTGATGCGCTCCTGCTCATGCGCTACGACTTTTGGCCAAATCACTTGCTTGCCGCAAAAAAGTATGGGACTACATTGGTG
CTTGCTGCTGCGGTGTTGCAACCCCAGAGCGCCTATTTTAATCCGTTACTGCGCCGCTTTTATAAAAAGCTTTTTCACCT
TTTTAATGCCATATACACCGTTGCGGAGCGCGATACACAAGCCTTTAAAGAGCATTTTGGTTACCGCAACGCTATAACTG
CTGGTGATCCTCGCTTTGACCAAGTGGTTGCTCGAAGTCGCAATAGAGCGGCTGTTGCAAATTTGCGGGCACACTACGAG
GGGCGCAAAGTGCTTGTGGCTGGCAGTGTGTGGGAAGCCGATGAGCAACTCCTTATTGCGGCATGGCAGGAACTCAATCC
ACGCCCATCACTTATTGTGGTGCCTCACCAAACTGAGCCTGAAAAAATTGCCCACTTGTGCTCTTTGCTTGATGAGCGCA
ACCTTTCCTACGCTCGTATTTCCACCTTTCCCGAAAGCTTTCAGCCTGAGCAGCAGATTCTCATTATTGATCAAATTGGC
TATCTTGCCGAGCTTTACTCAATAGCTTCAATTGCCTACGTTGGTGGTGGTTTTGGGGTGAATGTGCATAACACGTTGGA
GCCGGCCGTTTATGCTATTCCCGTGCTGTTTGGTCCAAACCACCACAACTCTCCAGAAGCGGCAGCTTTACTTGAAGCAG
GCGGCGCAACGGTGGTGCAACAGCAAAGCGAACTTCATGCAGCTTTGCAATGCCTTTGCTCTAACGAAAGCGAACGGCAA
CGCCAAGGCTCTGCGGCTGGCACTTTTGTACAAGCGCGCACCGGGGCAACGGCTATGGTGGTGGAGTATTTGGAGGGAGT
GGCGAATGTGGTTAAGTGGCAAGGGAGTTAA

Upstream 100 bases:

>100_bases
ATAGTACTATTTTTCCTCCTAAAAGAAGAAGGTTATTTTAAATATACTCTTTAGTTTGAAGGAATTGTGCGTTACCAGCT
TTCCCAACAACGCCTTACCA

Downstream 100 bases:

>100_bases
AAAGTGCAGGGAGTTGTCATTGTGCTGTGCAAAAGCATAATGCATTATTAAAAAAGATTTTAACAAACAATCAAAACAAA
CAAACCGAACCTATGGCAGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPISCHHMQSFPLYQTLFPLLAGVAQGVKALHPQIAAFFEVRQHLFTTLQQQLATMPNNGFRLWVHAASVGEFEQARPII
AALQARHPNLRLFISFLSPSGYNARKNFPNAAAVFYLPLDTAANARKLVALLKPDALLLMRYDFWPNHLLAAKKYGTTLV
LAAAVLQPQSAYFNPLLRRFYKKLFHLFNAIYTVAERDTQAFKEHFGYRNAITAGDPRFDQVVARSRNRAAVANLRAHYE
GRKVLVAGSVWEADEQLLIAAWQELNPRPSLIVVPHQTEPEKIAHLCSLLDERNLSYARISTFPESFQPEQQILIIDQIG
YLAELYSIASIAYVGGGFGVNVHNTLEPAVYAIPVLFGPNHHNSPEAAALLEAGGATVVQQQSELHAALQCLCSNESERQ
RQGSAAGTFVQARTGATAMVVEYLEGVANVVKWQGS

Sequences:

>Translated_436_residues
MPISCHHMQSFPLYQTLFPLLAGVAQGVKALHPQIAAFFEVRQHLFTTLQQQLATMPNNGFRLWVHAASVGEFEQARPII
AALQARHPNLRLFISFLSPSGYNARKNFPNAAAVFYLPLDTAANARKLVALLKPDALLLMRYDFWPNHLLAAKKYGTTLV
LAAAVLQPQSAYFNPLLRRFYKKLFHLFNAIYTVAERDTQAFKEHFGYRNAITAGDPRFDQVVARSRNRAAVANLRAHYE
GRKVLVAGSVWEADEQLLIAAWQELNPRPSLIVVPHQTEPEKIAHLCSLLDERNLSYARISTFPESFQPEQQILIIDQIG
YLAELYSIASIAYVGGGFGVNVHNTLEPAVYAIPVLFGPNHHNSPEAAALLEAGGATVVQQQSELHAALQCLCSNESERQ
RQGSAAGTFVQARTGATAMVVEYLEGVANVVKWQGS
>Mature_435_residues
PISCHHMQSFPLYQTLFPLLAGVAQGVKALHPQIAAFFEVRQHLFTTLQQQLATMPNNGFRLWVHAASVGEFEQARPIIA
ALQARHPNLRLFISFLSPSGYNARKNFPNAAAVFYLPLDTAANARKLVALLKPDALLLMRYDFWPNHLLAAKKYGTTLVL
AAAVLQPQSAYFNPLLRRFYKKLFHLFNAIYTVAERDTQAFKEHFGYRNAITAGDPRFDQVVARSRNRAAVANLRAHYEG
RKVLVAGSVWEADEQLLIAAWQELNPRPSLIVVPHQTEPEKIAHLCSLLDERNLSYARISTFPESFQPEQQILIIDQIGY
LAELYSIASIAYVGGGFGVNVHNTLEPAVYAIPVLFGPNHHNSPEAAALLEAGGATVVQQQSELHAALQCLCSNESERQR
QGSAAGTFVQARTGATAMVVEYLEGVANVVKWQGS

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=370, Percent_Identity=30.8108108108108, Blast_Score=135, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48351; Mature: 48220

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISCHHMQSFPLYQTLFPLLAGVAQGVKALHPQIAAFFEVRQHLFTTLQQQLATMPNNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRLWVHAASVGEFEQARPIIAALQARHPNLRLFISFLSPSGYNARKNFPNAAAVFYLPLD
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECC
TAANARKLVALLKPDALLLMRYDFWPNHLLAAKKYGTTLVLAAAVLQPQSAYFNPLLRRF
CCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
YKKLFHLFNAIYTVAERDTQAFKEHFGYRNAITAGDPRFDQVVARSRNRAAVANLRAHYE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCC
GRKVLVAGSVWEADEQLLIAAWQELNPRPSLIVVPHQTEPEKIAHLCSLLDERNLSYARI
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
STFPESFQPEQQILIIDQIGYLAELYSIASIAYVGGGFGVNVHNTLEPAVYAIPVLFGPN
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC
HHNSPEAAALLEAGGATVVQQQSELHAALQCLCSNESERQRQGSAAGTFVQARTGATAMV
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCHHHH
VEYLEGVANVVKWQGS
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PISCHHMQSFPLYQTLFPLLAGVAQGVKALHPQIAAFFEVRQHLFTTLQQQLATMPNNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FRLWVHAASVGEFEQARPIIAALQARHPNLRLFISFLSPSGYNARKNFPNAAAVFYLPLD
EEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEECC
TAANARKLVALLKPDALLLMRYDFWPNHLLAAKKYGTTLVLAAAVLQPQSAYFNPLLRRF
CCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHH
YKKLFHLFNAIYTVAERDTQAFKEHFGYRNAITAGDPRFDQVVARSRNRAAVANLRAHYE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCC
GRKVLVAGSVWEADEQLLIAAWQELNPRPSLIVVPHQTEPEKIAHLCSLLDERNLSYARI
CCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
STFPESFQPEQQILIIDQIGYLAELYSIASIAYVGGGFGVNVHNTLEPAVYAIPVLFGPN
CCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCC
HHNSPEAAALLEAGGATVVQQQSELHAALQCLCSNESERQRQGSAAGTFVQARTGATAMV
CCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCHHHH
VEYLEGVANVVKWQGS
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]