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The map label for this gene is btuB [H]

Identifier: 78188981

GI number: 78188981

Start: 1356053

End: 1357996

Strand: Reverse

Name: btuB [H]

Synonym: Cag_1014

Alternate gene names: 78188981

Gene position: 1357996-1356053 (Counterclockwise)

Preceding gene: 78188982

Following gene: 78188980

Centisome position: 52.8

GC content: 41.15

Gene sequence:

>1944_bases
ATGAACAAAAAAGTATTTGTGGTTGTCATGGCGGGTTTGCTATGCAGCAAGGGGCTTATGGCGGCTGATGGGCAGCCGAT
GAGCGTTATGGGTGAGGAGATGGTGGTTACTTCAAGCCGTTTTGAGGAGCCAAAGAAAAATCTCACATCGAACATTACCA
TTATCGGTAAAGATGAGATTGCTCAATCGTCGGCTCAAGATTTGGGGGAATTGTTGGCTGAAAAAAATCTTGGGCAAGTT
CAGAAATATCCCGGCACGATGACTTCGGTTGGTATTCGAGGTTTTAGAAGTGAAACGCATGGCAACGATTTAATGGGTAA
AGTTTTAGTGCTTATTGATGGTCGGCGTGCAGGTACGGGCAATACGGCTAAAATTATGACCGAGAATGTTGAGCGTATTG
AAATTATTCAAGGTCCAGCGGCAGTGCAATATGGTTCAGCCGCAATAGGTGGAGTAGTAAATGTTATTACTAAAAAAGGC
GATAATGTACCTTCATTTTTTGTAGAACAAAAAGGGGGAAGCAATGAATTTGTGAAAACGGCTGCCGGTGTGCAGGGCAA
AATTGGTAAGCTTGATTTTGCAAGTGCCCTTTCGCGCTCTGAAGCTGGTGATTATAAAACGGGTTCTGGTAAAACATATT
TTAATACGGGATATGATGAGCAGGTTTTAGCTAATCTTAATGTTGGCTATGAAATTGCTGATGGGCATCGTATCGGGTTT
GGATATCATTCATTTGATGTAGATAAAGCTGGATCGCCATCATATCTTAGCCTGAATGACTTACAAAGTTATACAAGCAA
TAACAATCATGCTATTGATGTTAGCTATGAAGGTGCTCTTACCAATAAACGTTGGTTATGGTCAACTCGCTATTTTAGTG
GTATGGATAGCTATCAATATGTTGATCCATCAACTTCTTATACAAGTTCGAGTGATGTGGAACAGCAAGGTGCTCAAGCG
CAAATTTCATTTACCGAAAAGAGCTTGCTTATAACGGCTGGAGTTGATTGGCTAAATTATGAATTAACCTCAACGTTAGC
CCCTAAGTGGAGTAAATACAATAACCCAGCCGTTTTTTTGCTTGCTAAATATGGAGTGTTTGAGGATCGGCTTTTGCTTT
CGGCTGGTGTTCGCTATGATGATTATAAGGTTGATTTACAACCTGCGGAAGGCACCTCGCGTAGTACGGATAATTTTGCC
CATCAAGTAGGAGCTGCATGGCAAGTGAATGATGTTGTAAAACTACGCGCTTCTTATGCCGAAGGTTTTAGAATGCCATC
GGCACGTGAACTTGCTGGAAATATTGTGTCGTTTGGTAAAACTTATATTGGAAATCCTAATTTAAACCCTGAAGTTAGCG
AAACATGGGAAACAGGTATTGATGTTGTATGGAGAGAGATTACATCATCACTTACATGGTTTTCAACCGATTACACCGAT
ATGATTGAAACCCAGCTCACTGCTCCTAAAACATATCTCTATAAAAATATTGGTAGTACTTCGTTGTCGGGTATTGAAGC
TGAATTTGCATGGAAAAGTTCTGCAACTACTTGGAACATTGAGCCTTACGTAAACTACAGCTATCTCTTAGAGCATAAAG
ATAATGCTACGGGCGATGATTTGCTTTATACCCCTGAGTGGAATGCCAGCACAGGAGTGCGTCTTCAGCATACGAATGGC
TTAAGTGCTGCACTGAATGTTACCGCAACGGGTAGTAGTAATGTGCAAGATTATGAAAGCAATTCAGGTAAAGTGATTAC
GAAGGGTGGCTTTAGTGTTGTAAATCTTTCAGCATCGAAAAAATTTACGCTTGATAAACAAGAGCGTCGTGCTATTACTA
TAAAAGCTGAGGTTGATAATTTGCTTGATCGCGATTATCAATACGTTAAAGGTTATCCCATGCCCGGACGCACGTTTGTG
ATTGGGTTGCGTGCCGACATCTAA

Upstream 100 bases:

>100_bases
ATTGTTTTTTTATAGAGAAATATTGTTGTGCAGAGTAAGTGCCTTTCTTCGCTATTCCTGTCAGTTATTTCGTTACAACT
GAAAACAGGTTACACAAACA

Downstream 100 bases:

>100_bases
TTAATATCGTTTCATTTTATCGCCGAGAAAGCATGGGGGCTTAGTGTGTGGGCTTCCATGCTTTTTTCGCCTAAATTATT
CATGAGTAACTCGATTTTGT

Product: TonB-dependent receptor-like protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MNKKVFVVVMAGLLCSKGLMAADGQPMSVMGEEMVVTSSRFEEPKKNLTSNITIIGKDEIAQSSAQDLGELLAEKNLGQV
QKYPGTMTSVGIRGFRSETHGNDLMGKVLVLIDGRRAGTGNTAKIMTENVERIEIIQGPAAVQYGSAAIGGVVNVITKKG
DNVPSFFVEQKGGSNEFVKTAAGVQGKIGKLDFASALSRSEAGDYKTGSGKTYFNTGYDEQVLANLNVGYEIADGHRIGF
GYHSFDVDKAGSPSYLSLNDLQSYTSNNNHAIDVSYEGALTNKRWLWSTRYFSGMDSYQYVDPSTSYTSSSDVEQQGAQA
QISFTEKSLLITAGVDWLNYELTSTLAPKWSKYNNPAVFLLAKYGVFEDRLLLSAGVRYDDYKVDLQPAEGTSRSTDNFA
HQVGAAWQVNDVVKLRASYAEGFRMPSARELAGNIVSFGKTYIGNPNLNPEVSETWETGIDVVWREITSSLTWFSTDYTD
MIETQLTAPKTYLYKNIGSTSLSGIEAEFAWKSSATTWNIEPYVNYSYLLEHKDNATGDDLLYTPEWNASTGVRLQHTNG
LSAALNVTATGSSNVQDYESNSGKVITKGGFSVVNLSASKKFTLDKQERRAITIKAEVDNLLDRDYQYVKGYPMPGRTFV
IGLRADI

Sequences:

>Translated_647_residues
MNKKVFVVVMAGLLCSKGLMAADGQPMSVMGEEMVVTSSRFEEPKKNLTSNITIIGKDEIAQSSAQDLGELLAEKNLGQV
QKYPGTMTSVGIRGFRSETHGNDLMGKVLVLIDGRRAGTGNTAKIMTENVERIEIIQGPAAVQYGSAAIGGVVNVITKKG
DNVPSFFVEQKGGSNEFVKTAAGVQGKIGKLDFASALSRSEAGDYKTGSGKTYFNTGYDEQVLANLNVGYEIADGHRIGF
GYHSFDVDKAGSPSYLSLNDLQSYTSNNNHAIDVSYEGALTNKRWLWSTRYFSGMDSYQYVDPSTSYTSSSDVEQQGAQA
QISFTEKSLLITAGVDWLNYELTSTLAPKWSKYNNPAVFLLAKYGVFEDRLLLSAGVRYDDYKVDLQPAEGTSRSTDNFA
HQVGAAWQVNDVVKLRASYAEGFRMPSARELAGNIVSFGKTYIGNPNLNPEVSETWETGIDVVWREITSSLTWFSTDYTD
MIETQLTAPKTYLYKNIGSTSLSGIEAEFAWKSSATTWNIEPYVNYSYLLEHKDNATGDDLLYTPEWNASTGVRLQHTNG
LSAALNVTATGSSNVQDYESNSGKVITKGGFSVVNLSASKKFTLDKQERRAITIKAEVDNLLDRDYQYVKGYPMPGRTFV
IGLRADI
>Mature_647_residues
MNKKVFVVVMAGLLCSKGLMAADGQPMSVMGEEMVVTSSRFEEPKKNLTSNITIIGKDEIAQSSAQDLGELLAEKNLGQV
QKYPGTMTSVGIRGFRSETHGNDLMGKVLVLIDGRRAGTGNTAKIMTENVERIEIIQGPAAVQYGSAAIGGVVNVITKKG
DNVPSFFVEQKGGSNEFVKTAAGVQGKIGKLDFASALSRSEAGDYKTGSGKTYFNTGYDEQVLANLNVGYEIADGHRIGF
GYHSFDVDKAGSPSYLSLNDLQSYTSNNNHAIDVSYEGALTNKRWLWSTRYFSGMDSYQYVDPSTSYTSSSDVEQQGAQA
QISFTEKSLLITAGVDWLNYELTSTLAPKWSKYNNPAVFLLAKYGVFEDRLLLSAGVRYDDYKVDLQPAEGTSRSTDNFA
HQVGAAWQVNDVVKLRASYAEGFRMPSARELAGNIVSFGKTYIGNPNLNPEVSETWETGIDVVWREITSSLTWFSTDYTD
MIETQLTAPKTYLYKNIGSTSLSGIEAEFAWKSSATTWNIEPYVNYSYLLEHKDNATGDDLLYTPEWNASTGVRLQHTNG
LSAALNVTATGSSNVQDYESNSGKVITKGGFSVVNLSASKKFTLDKQERRAITIKAEVDNLLDRDYQYVKGYPMPGRTFV
IGLRADI

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=673, Percent_Identity=24.517087667162, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1788478, Length=694, Percent_Identity=23.4870317002882, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1787723, Length=284, Percent_Identity=26.7605633802817, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786798, Length=185, Percent_Identity=30.8108108108108, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 70859; Mature: 70859

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKVFVVVMAGLLCSKGLMAADGQPMSVMGEEMVVTSSRFEEPKKNLTSNITIIGKDEI
CCCCEEEHEEHHHHHCCCCEECCCCCHHHCCCEEEEECHHHCCHHHHCCCCEEEEECHHH
AQSSAQDLGELLAEKNLGQVQKYPGTMTSVGIRGFRSETHGNDLMGKVLVLIDGRRAGTG
HHHHHHHHHHHHHHCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
NTAKIMTENVERIEIIQGPAAVQYGSAAIGGVVNVITKKGDNVPSFFVEQKGGSNEFVKT
CCEEEEECCCCEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
AAGVQGKIGKLDFASALSRSEAGDYKTGSGKTYFNTGYDEQVLANLNVGYEIADGHRIGF
HCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEECCCCEEEC
GYHSFDVDKAGSPSYLSLNDLQSYTSNNNHAIDVSYEGALTNKRWLWSTRYFSGMDSYQY
EEEECCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCEE
VDPSTSYTSSSDVEQQGAQAQISFTEKSLLITAGVDWLNYELTSTLAPKWSKYNNPAVFL
ECCCCCCCCCCCHHHCCCEEEEEEECCEEEEEECCCCEEEEEHHHCCCCCCCCCCCEEEE
LAKYGVFEDRLLLSAGVRYDDYKVDLQPAEGTSRSTDNFAHQVGAAWQVNDVVKLRASYA
EEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHHHHHHHH
EGFRMPSARELAGNIVSFGKTYIGNPNLNPEVSETWETGIDVVWREITSSLTWFSTDYTD
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHH
MIETQLTAPKTYLYKNIGSTSLSGIEAEFAWKSSATTWNIEPYVNYSYLLEHKDNATGDD
HHHHHCCCCHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEEECCCCCCCC
LLYTPEWNASTGVRLQHTNGLSAALNVTATGSSNVQDYESNSGKVITKGGFSVVNLSASK
EEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCCCCEEEEECCCEEEEECCCC
KFTLDKQERRAITIKAEVDNLLDRDYQYVKGYPMPGRTFVIGLRADI
EECCCCCCCEEEEEEEHHHHHHHCCHHHHCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MNKKVFVVVMAGLLCSKGLMAADGQPMSVMGEEMVVTSSRFEEPKKNLTSNITIIGKDEI
CCCCEEEHEEHHHHHCCCCEECCCCCHHHCCCEEEEECHHHCCHHHHCCCCEEEEECHHH
AQSSAQDLGELLAEKNLGQVQKYPGTMTSVGIRGFRSETHGNDLMGKVLVLIDGRRAGTG
HHHHHHHHHHHHHHCCCCHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCC
NTAKIMTENVERIEIIQGPAAVQYGSAAIGGVVNVITKKGDNVPSFFVEQKGGSNEFVKT
CCEEEEECCCCEEEEEECCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHH
AAGVQGKIGKLDFASALSRSEAGDYKTGSGKTYFNTGYDEQVLANLNVGYEIADGHRIGF
HCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCEEECCCCEEEC
GYHSFDVDKAGSPSYLSLNDLQSYTSNNNHAIDVSYEGALTNKRWLWSTRYFSGMDSYQY
EEEECCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCEE
VDPSTSYTSSSDVEQQGAQAQISFTEKSLLITAGVDWLNYELTSTLAPKWSKYNNPAVFL
ECCCCCCCCCCCHHHCCCEEEEEEECCEEEEEECCCCEEEEEHHHCCCCCCCCCCCEEEE
LAKYGVFEDRLLLSAGVRYDDYKVDLQPAEGTSRSTDNFAHQVGAAWQVNDVVKLRASYA
EEECCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCEEECHHHHHHHHHH
EGFRMPSARELAGNIVSFGKTYIGNPNLNPEVSETWETGIDVVWREITSSLTWFSTDYTD
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCHHH
MIETQLTAPKTYLYKNIGSTSLSGIEAEFAWKSSATTWNIEPYVNYSYLLEHKDNATGDD
HHHHHCCCCHHHHHHCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEEEEEECCCCCCCC
LLYTPEWNASTGVRLQHTNGLSAALNVTATGSSNVQDYESNSGKVITKGGFSVVNLSASK
EEECCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCCCCEEEEECCCEEEEECCCC
KFTLDKQERRAITIKAEVDNLLDRDYQYVKGYPMPGRTFVIGLRADI
EECCCCCCCEEEEEEEHHHHHHHCCHHHHCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]