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The map label for this gene is psrB [H]

Identifier: 78188921

GI number: 78188921

Start: 1290738

End: 1291337

Strand: Reverse

Name: psrB [H]

Synonym: Cag_0953

Alternate gene names: 78188921

Gene position: 1291337-1290738 (Counterclockwise)

Preceding gene: 78188922

Following gene: 78188920

Centisome position: 50.21

GC content: 50.67

Gene sequence:

>600_bases
ATGGCTCGTTATGGAATGGTAATGGATATGCGGAGTTGTGTTGGCTGCCAAGCCTGTTTGGCTGCCTGCGCAACCGAAAA
CCACACACCATTTTGGAGTGGTAAGTTCCGCACCCATGTGGAAGACAAGGAAAAAGGGAGTTACCCCAACGTGCGGCGCA
TTTTGCTACCTCGACTTTGTATGCACTGCGAAAACACACCGTGCATGTCAGCCTGCCCAACGGGGGCAACATGGAAAAAC
AAGGATGGCGTTATTTTAGTGAACTACGATCGCTGCATTGGCTGTTATGCCTGCTGCATTGCCTGCCCATACGATGCTCG
CTATGCCTACAACAACCATGATGTTGAAGAGGCTGAAAAGCTTTATGGTAAACTCTCAAGCCACACCATGCCGCATGTTG
ATAAATGCACCTTTTGCGACCATCGCATAGCCGCTGGACGCGAACCTGCGTGCGCCAGCACCTGCCCAACGCACTCACGC
ATTTTTGGCGACCTCGATAACCCTGCAAGCGAAGTACATCAACTTGCTGTAAGCGGTAAAGCTACAGCGTTGAATGCAGG
GCTTGGTACCTCACCCAAAGTATTTTACATCCCATCATAA

Upstream 100 bases:

>100_bases
TTCCTCACATAAAAAGCGTTGAAATAGAAATTGTGAGAGAAATAGCGGACAAGAATGTCCGCTCTCCTTTAATGTTTATC
AAATTTTAAACCCATACACT

Downstream 100 bases:

>100_bases
CGAAAGGAGAATTGCACTTATGACTTTTGTTCAACAAGAAGTTTGGCACTGGCAAATTGCCACTTACCTCTTTTTGGGTG
GGCTTGGCGGCGCCACCTTT

Product: polysulfide reductase, subunit B

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MARYGMVMDMRSCVGCQACLAACATENHTPFWSGKFRTHVEDKEKGSYPNVRRILLPRLCMHCENTPCMSACPTGATWKN
KDGVILVNYDRCIGCYACCIACPYDARYAYNNHDVEEAEKLYGKLSSHTMPHVDKCTFCDHRIAAGREPACASTCPTHSR
IFGDLDNPASEVHQLAVSGKATALNAGLGTSPKVFYIPS

Sequences:

>Translated_199_residues
MARYGMVMDMRSCVGCQACLAACATENHTPFWSGKFRTHVEDKEKGSYPNVRRILLPRLCMHCENTPCMSACPTGATWKN
KDGVILVNYDRCIGCYACCIACPYDARYAYNNHDVEEAEKLYGKLSSHTMPHVDKCTFCDHRIAAGREPACASTCPTHSR
IFGDLDNPASEVHQLAVSGKATALNAGLGTSPKVFYIPS
>Mature_198_residues
ARYGMVMDMRSCVGCQACLAACATENHTPFWSGKFRTHVEDKEKGSYPNVRRILLPRLCMHCENTPCMSACPTGATWKNK
DGVILVNYDRCIGCYACCIACPYDARYAYNNHDVEEAEKLYGKLSSHTMPHVDKCTFCDHRIAAGREPACASTCPTHSRI
FGDLDNPASEVHQLAVSGKATALNAGLGTSPKVFYIPS

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=199, Percent_Identity=36.1809045226131, Blast_Score=134, Evalue=3e-33,
Organism=Escherichia coli, GI226510944, Length=171, Percent_Identity=35.672514619883, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1787122, Length=182, Percent_Identity=34.0659340659341, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1787872, Length=206, Percent_Identity=33.0097087378641, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787740, Length=183, Percent_Identity=27.8688524590164, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1787749, Length=177, Percent_Identity=28.8135593220339, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1787478, Length=131, Percent_Identity=33.587786259542, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789370, Length=200, Percent_Identity=28.5, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1790326, Length=206, Percent_Identity=29.126213592233, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789067, Length=177, Percent_Identity=29.9435028248588, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082180, Length=157, Percent_Identity=31.8471337579618, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082179, Length=156, Percent_Identity=28.8461538461538, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI87082114, Length=201, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1789079, Length=106, Percent_Identity=34.9056603773585, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 21882; Mature: 21751

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
12.1 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
11.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYGMVMDMRSCVGCQACLAACATENHTPFWSGKFRTHVEDKEKGSYPNVRRILLPRLC
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
MHCENTPCMSACPTGATWKNKDGVILVNYDRCIGCYACCIACPYDARYAYNNHDVEEAEK
HHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LYGKLSSHTMPHVDKCTFCDHRIAAGREPACASTCPTHSRIFGDLDNPASEVHQLAVSGK
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCC
ATALNAGLGTSPKVFYIPS
CEEEECCCCCCCEEEEECC
>Mature Secondary Structure 
ARYGMVMDMRSCVGCQACLAACATENHTPFWSGKFRTHVEDKEKGSYPNVRRILLPRLC
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
MHCENTPCMSACPTGATWKNKDGVILVNYDRCIGCYACCIACPYDARYAYNNHDVEEAEK
HHCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LYGKLSSHTMPHVDKCTFCDHRIAAGREPACASTCPTHSRIFGDLDNPASEVHQLAVSGK
HHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCC
ATALNAGLGTSPKVFYIPS
CEEEECCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]