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The map label for this gene is livK [C]

Identifier: 78188823

GI number: 78188823

Start: 1183878

End: 1185023

Strand: Reverse

Name: livK [C]

Synonym: Cag_0853

Alternate gene names: 78188823

Gene position: 1185023-1183878 (Counterclockwise)

Preceding gene: 78188824

Following gene: 78188822

Centisome position: 46.07

GC content: 45.11

Gene sequence:

>1146_bases
ATGAAAATCAGCTTTATCTCACTTGTTGCTGGCGCTATGCTGGCTGTGCCAATGGTCGCTCAAGCGGCTCCCATTAAAAT
TGGCTTTATTAACTCCATTACTGGGAAAGAAGCCCAAATTGGTGAAAACCTTACCAACGGTGCTACCATGGCTGTTGAGG
ATATGAAAGCCAAAGGTTATCAATTTGAAGTGATTCGTGAAGATGATGGTGGCGATCCCAAAAATGCTTTAGCGGCTTAC
GAAAAGCTTGTAACGCGCGATGGCGTTATTGGTGTGGTGGGACCTTACACGTCGGGTAGTGCTAACGTGGTTGCTTCGCG
TGCTGACCAGTATCAAGTGCCGCTTTTGGTGCCTGCTGCTGCTAAGGAAGAAATTACCATGAAAGGTTACAAAAATGTGT
TCCGCCTTAATGCTCCTGCCAACGTATATTCTAAAGTGTTGATGGATGCAGTAAAAGCTTACAAGCCAAAAACCATTGCT
TACATTTATGCGGCTACCGATTTTGGTGTGTCAACGGTAAAAACTGCTCAGGAAAATGCTGCAAAAATGGGACTTACTGA
GGTTGCTAACGAAAAGTATCAGCAAGGGCAGCCTGATTACCGTCCATCACTTTCCAAAGTAAAAGCGGCTAACCCCGACC
TTGTATTCCTTGTTTCCTACGATGCCGATGCCATTTTGTTAATGCGTCAAGCAAAAGAACTTGGGTTAAAGCCAAAAGCC
TTCCTTGGTGCTGGTGCTGGTTACACCACCGCTCAATTTGCCCAGCAAACTGAAATTTCAAACCTTGTTGTTTCTGCAAC
GCAGTGGACGGATGATGTAAATTGGGCTGGTGCGGCTGATTTTTCTCGCCGTTACAAAGCTAAATTTGCTAAAGAGCCTT
CGTACCATGCGGCTTGCGCTTACGAGGCAATGCGCATTATGATTGATACGGCTACTCGTCAAAAGAGTGCGCCAAAAGTT
CGCACGGCATTGAAAGCTGGTAGTTGGAATGGCATTATGGGTCCCGTAAAGTTTGCGAACTATGCAGGCTTTACCAACCA
GAACAACCATCCTATGCTTGTACAGCAGATTCAAGCAGGCAAATATGAAACGGTTTATCCTGCACAATTCAGCAAAAAGA
AATTGGTTTATCCATTTACTCGCTAA

Upstream 100 bases:

>100_bases
AATCCATTGCAGAGTTTTGTAAAAGCTTTTTTCTAAACCAATAGCATCCAATCCCGTGCTCATTACGGGATTTCACTTAA
CCAAAAGGAGAGGCGATTCC

Downstream 100 bases:

>100_bases
TTGTTAGCTAAGCAGTTTTTTTTCAGCACACTGCTTTTTTTGAATTGCTCTCCTTACTTGGCTTAAACAGATTATTTGTA
TCGGTTACCAATAGCAGTAA

Product: branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKISFISLVAGAMLAVPMVAQAAPIKIGFINSITGKEAQIGENLTNGATMAVEDMKAKGYQFEVIREDDGGDPKNALAAY
EKLVTRDGVIGVVGPYTSGSANVVASRADQYQVPLLVPAAAKEEITMKGYKNVFRLNAPANVYSKVLMDAVKAYKPKTIA
YIYAATDFGVSTVKTAQENAAKMGLTEVANEKYQQGQPDYRPSLSKVKAANPDLVFLVSYDADAILLMRQAKELGLKPKA
FLGAGAGYTTAQFAQQTEISNLVVSATQWTDDVNWAGAADFSRRYKAKFAKEPSYHAACAYEAMRIMIDTATRQKSAPKV
RTALKAGSWNGIMGPVKFANYAGFTNQNNHPMLVQQIQAGKYETVYPAQFSKKKLVYPFTR

Sequences:

>Translated_381_residues
MKISFISLVAGAMLAVPMVAQAAPIKIGFINSITGKEAQIGENLTNGATMAVEDMKAKGYQFEVIREDDGGDPKNALAAY
EKLVTRDGVIGVVGPYTSGSANVVASRADQYQVPLLVPAAAKEEITMKGYKNVFRLNAPANVYSKVLMDAVKAYKPKTIA
YIYAATDFGVSTVKTAQENAAKMGLTEVANEKYQQGQPDYRPSLSKVKAANPDLVFLVSYDADAILLMRQAKELGLKPKA
FLGAGAGYTTAQFAQQTEISNLVVSATQWTDDVNWAGAADFSRRYKAKFAKEPSYHAACAYEAMRIMIDTATRQKSAPKV
RTALKAGSWNGIMGPVKFANYAGFTNQNNHPMLVQQIQAGKYETVYPAQFSKKKLVYPFTR
>Mature_381_residues
MKISFISLVAGAMLAVPMVAQAAPIKIGFINSITGKEAQIGENLTNGATMAVEDMKAKGYQFEVIREDDGGDPKNALAAY
EKLVTRDGVIGVVGPYTSGSANVVASRADQYQVPLLVPAAAKEEITMKGYKNVFRLNAPANVYSKVLMDAVKAYKPKTIA
YIYAATDFGVSTVKTAQENAAKMGLTEVANEKYQQGQPDYRPSLSKVKAANPDLVFLVSYDADAILLMRQAKELGLKPKA
FLGAGAGYTTAQFAQQTEISNLVVSATQWTDDVNWAGAADFSRRYKAKFAKEPSYHAACAYEAMRIMIDTATRQKSAPKV
RTALKAGSWNGIMGPVKFANYAGFTNQNNHPMLVQQIQAGKYETVYPAQFSKKKLVYPFTR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=252, Percent_Identity=28.1746031746032, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789867, Length=352, Percent_Identity=25.8522727272727, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41357; Mature: 41357

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISFISLVAGAMLAVPMVAQAAPIKIGFINSITGKEAQIGENLTNGATMAVEDMKAKGY
CEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEEHHHCCCE
QFEVIREDDGGDPKNALAAYEKLVTRDGVIGVVGPYTSGSANVVASRADQYQVPLLVPAA
EEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEECCEEECCC
AKEEITMKGYKNVFRLNAPANVYSKVLMDAVKAYKPKTIAYIYAATDFGVSTVKTAQENA
CCCCEEHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
AKMGLTEVANEKYQQGQPDYRPSLSKVKAANPDLVFLVSYDADAILLMRQAKELGLKPKA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHH
FLGAGAGYTTAQFAQQTEISNLVVSATQWTDDVNWAGAADFSRRYKAKFAKEPSYHAACA
HEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
YEAMRIMIDTATRQKSAPKVRTALKAGSWNGIMGPVKFANYAGFTNQNNHPMLVQQIQAG
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHCC
KYETVYPAQFSKKKLVYPFTR
CCCEECCCCCCCCEEECCCCC
>Mature Secondary Structure
MKISFISLVAGAMLAVPMVAQAAPIKIGFINSITGKEAQIGENLTNGATMAVEDMKAKGY
CEEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCEEEEEHHHCCCE
QFEVIREDDGGDPKNALAAYEKLVTRDGVIGVVGPYTSGSANVVASRADQYQVPLLVPAA
EEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCEECCEEECCC
AKEEITMKGYKNVFRLNAPANVYSKVLMDAVKAYKPKTIAYIYAATDFGVSTVKTAQENA
CCCCEEHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHH
AKMGLTEVANEKYQQGQPDYRPSLSKVKAANPDLVFLVSYDADAILLMRQAKELGLKPKA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCHH
FLGAGAGYTTAQFAQQTEISNLVVSATQWTDDVNWAGAADFSRRYKAKFAKEPSYHAACA
HEECCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
YEAMRIMIDTATRQKSAPKVRTALKAGSWNGIMGPVKFANYAGFTNQNNHPMLVQQIQAG
HHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHCC
KYETVYPAQFSKKKLVYPFTR
CCCEECCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA