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The map label for this gene is phoA [H]

Identifier: 78188758

GI number: 78188758

Start: 1104851

End: 1106185

Strand: Reverse

Name: phoA [H]

Synonym: Cag_0787

Alternate gene names: 78188758

Gene position: 1106185-1104851 (Counterclockwise)

Preceding gene: 78188759

Following gene: 78188757

Centisome position: 43.01

GC content: 44.72

Gene sequence:

>1335_bases
ATGTTCTCTAAGAAAATAATTCGTGACGCTGGTATTGGCTTATTTTTATTGGGGTTAAGTAATGGTGCTCATGCCGTTAC
CCATTTAGCTTCTGTACCAAAACCAAAAATTGGAAATGTAATTTTTATGCATCCTGATGGTACCAGTGTTTCGCATTGGA
CGGCTGCTCGTATGGCTATTGCTGGACCTGATGGCACCATCAATTGGGATCGCTTACCTGCAATGGCTGTGTATCGTGGA
CACATGAAAAATTCGACTGGCGCAACATCACATGGTGGAGCAACAACCCATGCTTTTGGGGTAAAAGTAGATGCTGATTC
GTATGGCATGGATCGTGATCGCGCGCTTACTGCTCTTTCGGGTAAACCAATGAGCATTATGCGCGAGGCTCTCGCTGCTG
GATTAGCGGTTGGAGTGGTTAATTCAGGTAATATTGATGAGCCCGGTACAGGCGTATTTTTAGCTTCATCGCGTAGCCGT
AAGGATGGGCAAGCAATTGCTGAGCAAATAATTTACAGTGGTGCGCAGGTTATTATGTCGGGAGGTGAGCGTTGGCTTTT
GCCGAAAGGGGTTAAAGGGCGTCATGGAGAGGGTGAGCGCAGTGATGGCAAAAATCTTATTGATGTAGCACAGACTCTTG
GTTATACTATTGTGTATAACAGAGATGAATTAGCAAATCTTCCTAAAGATACGAAGCGGGTACTTGGTGTATTTGCACAT
CATCATACTTTTTTTGATCACCCTGAAGAGGAATTACAAAAGCAAAAATTACCACTCTATGTTGCCAATGCTCCAACAGT
TGGTGAAATGACCGATGCTGCGTTGCGTATTTTATCGAATCAGGGGAAACGGTTTTTATTAGTGGTTGAAGAAGAGGGAA
CGGATAATTTTGGGAATGTGAATAATGCCGTAGGTACCCTTGAGGCGCTTCGTCGTGCTGATGCTGCTTACGGGGTTGCG
CGTTCTTATGTACAACGCAATCCTGCAACATTGTTGCTTACGGCTGCTGATAGTGATGCAAGTGGTTTAGAGGTTGTGTC
GGTTGCTCCTGATGTAGCAATGAAGCCACTGAATAAAACCACGCATAATGGTGCACCTTTAGATGGACGTACAGGTACAC
AATCGTTGCCATTTATTGCCGAAGCCGATCAATTTGGTAATCGCCTACCTTTTGGTATTGCTTGGACTGATATGAACGAT
GGGGCTGGTGGTATTCTTGCGCGAGCTGAGGGCATAAATTCTGAAGCTTTACGAAGTGGTAGTTGCGATAATAGCGATGT
GTATCGCCTTATGTACCTAACACTTTTTGGACGTTCAGCCGATAGCAAGAAGTAA

Upstream 100 bases:

>100_bases
TCAAACAATTAACAATCAACATCGTAAAGTCTTTGTAAGTTAGTTGCTGGTATTCAATAAATACGCATAAAACACATTAA
TGCAAATTGGAGGGTTATGT

Downstream 100 bases:

>100_bases
CAGGTAAAAGGGGCATTTACCCCCTTCGCTGCAACTACGGTAATGCCGAGTACCTAAAACTTAGTTAAACATCCCGTTTA
GTGGTATGGTGGTGCTCTAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MFSKKIIRDAGIGLFLLGLSNGAHAVTHLASVPKPKIGNVIFMHPDGTSVSHWTAARMAIAGPDGTINWDRLPAMAVYRG
HMKNSTGATSHGGATTHAFGVKVDADSYGMDRDRALTALSGKPMSIMREALAAGLAVGVVNSGNIDEPGTGVFLASSRSR
KDGQAIAEQIIYSGAQVIMSGGERWLLPKGVKGRHGEGERSDGKNLIDVAQTLGYTIVYNRDELANLPKDTKRVLGVFAH
HHTFFDHPEEELQKQKLPLYVANAPTVGEMTDAALRILSNQGKRFLLVVEEEGTDNFGNVNNAVGTLEALRRADAAYGVA
RSYVQRNPATLLLTAADSDASGLEVVSVAPDVAMKPLNKTTHNGAPLDGRTGTQSLPFIAEADQFGNRLPFGIAWTDMND
GAGGILARAEGINSEALRSGSCDNSDVYRLMYLTLFGRSADSKK

Sequences:

>Translated_444_residues
MFSKKIIRDAGIGLFLLGLSNGAHAVTHLASVPKPKIGNVIFMHPDGTSVSHWTAARMAIAGPDGTINWDRLPAMAVYRG
HMKNSTGATSHGGATTHAFGVKVDADSYGMDRDRALTALSGKPMSIMREALAAGLAVGVVNSGNIDEPGTGVFLASSRSR
KDGQAIAEQIIYSGAQVIMSGGERWLLPKGVKGRHGEGERSDGKNLIDVAQTLGYTIVYNRDELANLPKDTKRVLGVFAH
HHTFFDHPEEELQKQKLPLYVANAPTVGEMTDAALRILSNQGKRFLLVVEEEGTDNFGNVNNAVGTLEALRRADAAYGVA
RSYVQRNPATLLLTAADSDASGLEVVSVAPDVAMKPLNKTTHNGAPLDGRTGTQSLPFIAEADQFGNRLPFGIAWTDMND
GAGGILARAEGINSEALRSGSCDNSDVYRLMYLTLFGRSADSKK
>Mature_444_residues
MFSKKIIRDAGIGLFLLGLSNGAHAVTHLASVPKPKIGNVIFMHPDGTSVSHWTAARMAIAGPDGTINWDRLPAMAVYRG
HMKNSTGATSHGGATTHAFGVKVDADSYGMDRDRALTALSGKPMSIMREALAAGLAVGVVNSGNIDEPGTGVFLASSRSR
KDGQAIAEQIIYSGAQVIMSGGERWLLPKGVKGRHGEGERSDGKNLIDVAQTLGYTIVYNRDELANLPKDTKRVLGVFAH
HHTFFDHPEEELQKQKLPLYVANAPTVGEMTDAALRILSNQGKRFLLVVEEEGTDNFGNVNNAVGTLEALRRADAAYGVA
RSYVQRNPATLLLTAADSDASGLEVVSVAPDVAMKPLNKTTHNGAPLDGRTGTQSLPFIAEADQFGNRLPFGIAWTDMND
GAGGILARAEGINSEALRSGSCDNSDVYRLMYLTLFGRSADSKK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=377, Percent_Identity=25.4641909814324, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157266292, Length=288, Percent_Identity=27.4305555555556, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI294660770, Length=370, Percent_Identity=25.1351351351351, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI157266296, Length=288, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI94721246, Length=288, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI294660772, Length=344, Percent_Identity=24.4186046511628, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI48994877, Length=359, Percent_Identity=27.0194986072423, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI85725200, Length=330, Percent_Identity=29.0909090909091, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI85815833, Length=330, Percent_Identity=29.0909090909091, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585213, Length=343, Percent_Identity=27.4052478134111, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355151, Length=285, Percent_Identity=24.9122807017544, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 47296; Mature: 47296

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKKIIRDAGIGLFLLGLSNGAHAVTHLASVPKPKIGNVIFMHPDGTSVSHWTAARMAI
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHEEEEEE
AGPDGTINWDRLPAMAVYRGHMKNSTGATSHGGATTHAFGVKVDADSYGMDRDRALTALS
ECCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHEEEECC
GKPMSIMREALAAGLAVGVVNSGNIDEPGTGVFLASSRSRKDGQAIAEQIIYSGAQVIMS
CCCHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEC
GGERWLLPKGVKGRHGEGERSDGKNLIDVAQTLGYTIVYNRDELANLPKDTKRVLGVFAH
CCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHCCCHHHHHHHHHHHH
HHTFFDHPEEELQKQKLPLYVANAPTVGEMTDAALRILSNQGKRFLLVVEEEGTDNFGNV
HCHHCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NNAVGTLEALRRADAAYGVARSYVQRNPATLLLTAADSDASGLEVVSVAPDVAMKPLNKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHCCCCCC
THNGAPLDGRTGTQSLPFIAEADQFGNRLPFGIAWTDMNDGAGGILARAEGINSEALRSG
CCCCCCCCCCCCCCCCCCEECHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHCC
SCDNSDVYRLMYLTLFGRSADSKK
CCCCCHHHHHEEHHHHCCCCCCCC
>Mature Secondary Structure
MFSKKIIRDAGIGLFLLGLSNGAHAVTHLASVPKPKIGNVIFMHPDGTSVSHWTAARMAI
CCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHEEEEEE
AGPDGTINWDRLPAMAVYRGHMKNSTGATSHGGATTHAFGVKVDADSYGMDRDRALTALS
ECCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHEEEECC
GKPMSIMREALAAGLAVGVVNSGNIDEPGTGVFLASSRSRKDGQAIAEQIIYSGAQVIMS
CCCHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEC
GGERWLLPKGVKGRHGEGERSDGKNLIDVAQTLGYTIVYNRDELANLPKDTKRVLGVFAH
CCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCHHHHCCCHHHHHHHHHHHH
HHTFFDHPEEELQKQKLPLYVANAPTVGEMTDAALRILSNQGKRFLLVVEEEGTDNFGNV
HCHHCCCCHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
NNAVGTLEALRRADAAYGVARSYVQRNPATLLLTAADSDASGLEVVSVAPDVAMKPLNKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCHHHCCCCCC
THNGAPLDGRTGTQSLPFIAEADQFGNRLPFGIAWTDMNDGAGGILARAEGINSEALRSG
CCCCCCCCCCCCCCCCCCEECHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCHHHHHCC
SCDNSDVYRLMYLTLFGRSADSKK
CCCCCHHHHHEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]