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The map label for this gene is cca [H]

Identifier: 78188698

GI number: 78188698

Start: 996712

End: 998142

Strand: Reverse

Name: cca [H]

Synonym: Cag_0722

Alternate gene names: 78188698

Gene position: 998142-996712 (Counterclockwise)

Preceding gene: 78188699

Following gene: 78188697

Centisome position: 38.81

GC content: 49.13

Gene sequence:

>1431_bases
ATGAAAGCACTTCCCGCCATTACACCTTTTGCAATTCCTGATATTCTCTGCCGTATTGGCGATATAGCCGATCGTGAAGA
TGCCGCCTGCTACCTTGTGGGCGGCTACGTGCGCGACCTCATGATGAAACGCCCTTGCACCGATGTTGATATTATGATTA
CGGGCGATCCCGTACCGTTTGCCCACATGGTGCAAGAAGAGCTACAAGGGCGTAACTTTGTACTCTTTGAACGCTTTCGT
ACCGCACAATTGGAGCTTGATGATCCCAACTTAGGAACGTTTAAAGTAGAATTAGTGGGCGCTCGAAAAGAGTCGTACAA
TAGCGATTCCCGCAAACCCATCACCCTTGTTGGCACGCTTGAGGACGATTTAATGCGGCGCGATTTTACCATTAACGCCC
TCGCTATGCGCCTCAATCGCGAAGAACGCTATACGGTAACAGACCTCTTTAACGGCATGGAGGATATGGAGGCAAAAATC
CTCCGCACACCGTTAGAGCCATTACAAACCTTCTCAGACGATCCGTTGCGCATGATGCGTGCGGCTCGCTTTGCCGCCCA
GCTTGATTTTACCCTTCTGCCCAATGCGCTTGAGGCAATTCGAGCCATGCACGAGCGCATTCATATTGTCTCAATGGAAC
GGGTAAGCCACGAATTTCTTAAAATTATGCGCACCCATCGCCCCTCTGTTGGCTTGCTTATTTTGCATGAAACAGGATTA
TTGAAAGAACTGCTGCCCGAAGTATCGGCTATGGCTGGTATTGAGCAAGTTGATGGCATGGGGCATAAGGATACGCTATT
CCACACCATGCAAGTGGTTGATACCGTAGCAACCTATTCCGATAACCTTTGGCTACGCATTGCCGCCCTGCTGCACGATG
TTGCCAAACCCGTTACCAAACGCTTTTCCAAACAGAGCGGCTGGACATTCCACGGGCACGATGCCGTAGGCATTAAAATG
GTAGCAAAAATTTTTCGCACCATGCGCTGGTCGCACGAGCATCTTGAGTATGTGCAAAAATTAGTACGCCTGCACCATCG
CCCCATTCCCCTTTCCAAAGAGGAAATTACCGACTCAGCCGTGCGCCGCCTTATGTTTGATGCAGGTGAAGATTTACGCG
ACTTAATGACGCTCTGCCGCTCCGATGTTACCAGCAAAAATCCCCGCAAAGTGGCACGCATTATGAGCAACTTCAACCAC
GTTGAAGCTAAAATTGCCGAAGTGGGCGAAAAAGATCAGCTTGCAAAATGGCGCCCACCCATTAGCGGACAAGATATTAT
GGAGGCACTTGGTTTAGCAGAAGGCAAAGCGGTGGGAAAGATTAAAAAGCAGATGGAAAATGCGGTGATTGATGGCATTA
TTGCTTATGATCGTGAAGCTGCGTTAGCGTATGTAAGGAAAGTGTATGAGGAGATGAAGGGAGAGACTTGA

Upstream 100 bases:

>100_bases
ACGGGCGCCGTGCATTGTTTGTTGCAGGCAAAATTACCGATGAAATTAATGCCTCCACTGCCCTGCTTCACGATTAAGCC
CTCTCTTTCTTGATTGCCTT

Downstream 100 bases:

>100_bases
GGGATGAGAAAATAGTGATGCGGGGATACATTGTATCAAGAAAACCTCTGCAATAGCCATTTATTGTCATTCCCGCGATG
GTTACTGAGCTTGTCGAAGT

Product: hypothetical protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKALPAITPFAIPDILCRIGDIADREDAACYLVGGYVRDLMMKRPCTDVDIMITGDPVPFAHMVQEELQGRNFVLFERFR
TAQLELDDPNLGTFKVELVGARKESYNSDSRKPITLVGTLEDDLMRRDFTINALAMRLNREERYTVTDLFNGMEDMEAKI
LRTPLEPLQTFSDDPLRMMRAARFAAQLDFTLLPNALEAIRAMHERIHIVSMERVSHEFLKIMRTHRPSVGLLILHETGL
LKELLPEVSAMAGIEQVDGMGHKDTLFHTMQVVDTVATYSDNLWLRIAALLHDVAKPVTKRFSKQSGWTFHGHDAVGIKM
VAKIFRTMRWSHEHLEYVQKLVRLHHRPIPLSKEEITDSAVRRLMFDAGEDLRDLMTLCRSDVTSKNPRKVARIMSNFNH
VEAKIAEVGEKDQLAKWRPPISGQDIMEALGLAEGKAVGKIKKQMENAVIDGIIAYDREAALAYVRKVYEEMKGET

Sequences:

>Translated_476_residues
MKALPAITPFAIPDILCRIGDIADREDAACYLVGGYVRDLMMKRPCTDVDIMITGDPVPFAHMVQEELQGRNFVLFERFR
TAQLELDDPNLGTFKVELVGARKESYNSDSRKPITLVGTLEDDLMRRDFTINALAMRLNREERYTVTDLFNGMEDMEAKI
LRTPLEPLQTFSDDPLRMMRAARFAAQLDFTLLPNALEAIRAMHERIHIVSMERVSHEFLKIMRTHRPSVGLLILHETGL
LKELLPEVSAMAGIEQVDGMGHKDTLFHTMQVVDTVATYSDNLWLRIAALLHDVAKPVTKRFSKQSGWTFHGHDAVGIKM
VAKIFRTMRWSHEHLEYVQKLVRLHHRPIPLSKEEITDSAVRRLMFDAGEDLRDLMTLCRSDVTSKNPRKVARIMSNFNH
VEAKIAEVGEKDQLAKWRPPISGQDIMEALGLAEGKAVGKIKKQMENAVIDGIIAYDREAALAYVRKVYEEMKGET
>Mature_476_residues
MKALPAITPFAIPDILCRIGDIADREDAACYLVGGYVRDLMMKRPCTDVDIMITGDPVPFAHMVQEELQGRNFVLFERFR
TAQLELDDPNLGTFKVELVGARKESYNSDSRKPITLVGTLEDDLMRRDFTINALAMRLNREERYTVTDLFNGMEDMEAKI
LRTPLEPLQTFSDDPLRMMRAARFAAQLDFTLLPNALEAIRAMHERIHIVSMERVSHEFLKIMRTHRPSVGLLILHETGL
LKELLPEVSAMAGIEQVDGMGHKDTLFHTMQVVDTVATYSDNLWLRIAALLHDVAKPVTKRFSKQSGWTFHGHDAVGIKM
VAKIFRTMRWSHEHLEYVQKLVRLHHRPIPLSKEEITDSAVRRLMFDAGEDLRDLMTLCRSDVTSKNPRKVARIMSNFNH
VEAKIAEVGEKDQLAKWRPPISGQDIMEALGLAEGKAVGKIKKQMENAVIDGIIAYDREAALAYVRKVYEEMKGET

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=328, Percent_Identity=32.3170731707317, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI87081691, Length=309, Percent_Identity=26.537216828479, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321016, Length=230, Percent_Identity=30.8695652173913, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54209; Mature: 54209

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALPAITPFAIPDILCRIGDIADREDAACYLVGGYVRDLMMKRPCTDVDIMITGDPVPF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCH
AHMVQEELQGRNFVLFERFRTAQLELDDPNLGTFKVELVGARKESYNSDSRKPITLVGTL
HHHHHHHHCCCCEEEEEHHCCCEEEECCCCCCEEEEEEEECHHHCCCCCCCCCEEEEECC
EDDLMRRDFTINALAMRLNREERYTVTDLFNGMEDMEAKILRTPLEPLQTFSDDPLRMMR
HHHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHCCHHHHHCCCCCHHHHHH
AARFAAQLDFTLLPNALEAIRAMHERIHIVSMERVSHEFLKIMRTHRPSVGLLILHETGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
LKELLPEVSAMAGIEQVDGMGHKDTLFHTMQVVDTVATYSDNLWLRIAALLHDVAKPVTK
HHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RFSKQSGWTFHGHDAVGIKMVAKIFRTMRWSHEHLEYVQKLVRLHHRPIPLSKEEITDSA
HHHHCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VRRLMFDAGEDLRDLMTLCRSDVTSKNPRKVARIMSNFNHVEAKIAEVGEKDQLAKWRPP
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHCHHHCCCC
ISGQDIMEALGLAEGKAVGKIKKQMENAVIDGIIAYDREAALAYVRKVYEEMKGET
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKALPAITPFAIPDILCRIGDIADREDAACYLVGGYVRDLMMKRPCTDVDIMITGDPVPF
CCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEECCCCCH
AHMVQEELQGRNFVLFERFRTAQLELDDPNLGTFKVELVGARKESYNSDSRKPITLVGTL
HHHHHHHHCCCCEEEEEHHCCCEEEECCCCCCEEEEEEEECHHHCCCCCCCCCEEEEECC
EDDLMRRDFTINALAMRLNREERYTVTDLFNGMEDMEAKILRTPLEPLQTFSDDPLRMMR
HHHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCHHHHHHHHHHCCHHHHHCCCCCHHHHHH
AARFAAQLDFTLLPNALEAIRAMHERIHIVSMERVSHEFLKIMRTHRPSVGLLILHETGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCH
LKELLPEVSAMAGIEQVDGMGHKDTLFHTMQVVDTVATYSDNLWLRIAALLHDVAKPVTK
HHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RFSKQSGWTFHGHDAVGIKMVAKIFRTMRWSHEHLEYVQKLVRLHHRPIPLSKEEITDSA
HHHHCCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
VRRLMFDAGEDLRDLMTLCRSDVTSKNPRKVARIMSNFNHVEAKIAEVGEKDQLAKWRPP
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHCHHHCCCC
ISGQDIMEALGLAEGKAVGKIKKQMENAVIDGIIAYDREAALAYVRKVYEEMKGET
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA