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The map label for this gene is 78188099

Identifier: 78188099

GI number: 78188099

Start: 130592

End: 131977

Strand: Reverse

Name: 78188099

Synonym: Cag_0116

Alternate gene names: NA

Gene position: 131977-130592 (Counterclockwise)

Preceding gene: 78188100

Following gene: 78188098

Centisome position: 5.13

GC content: 41.13

Gene sequence:

>1386_bases
ATGGGTAATTCTGTCACTCAAAACGGAAAGTCACTTAATATTCTCCTTATTGCCCCAAAAGGAAAAAAAGATTCAAAATC
GAACCAGAAGCCATTGTTTAATATGGCAATTGGGGTGCTGGTGAGTATTACTCCACCACAGCATCATCTTGAAATTATTG
ACGAACATTTTGGTGACGAAATTAACTACGATGGTAAGTATGACCTCGTTGGCATAACCTCTCGCACCATTGATGCTACA
CGCGCCTATGAGATTGCCGATACATTTCGCGCTAAAGGCAAAAAGGTCATTTTAGGTGGTTTGCATGTATCGTTTAACAA
AGAGGAAGCACGAGCACATGCCGACTGTATTGTGTGTGGCGAAGCTGAAAATTTGTGGTCAACGCTGCTTGATGATGCTG
CGAACAACGCTTTAAAACCCGATTATGACTCAAAGGATTTCCCCTCTGTAACAGAAATTGTGCCTATTGATTATGCCAAA
ATCGCAAAAGTTTCAAAACGCGAAAAGGTAGATGGCACAAAATCCATCCCCATTTATATTACACGCGGCTGCCCGTTTGA
GTGTTCATTTTGCGTAACCCCCAATTTTACAGGGAAGCTTTATCGTGCCCAAAAGCCCGATGATTTAAAGCGTCAAATTG
AAGAGGCAAAACGCGTCTTTTTTAAAGCAAACAGTAAAACCGCAAAACCGTGGTTTATGCTGTGCGACGAAAACCTTGGC
GTCAGCAAAAAACGCTTATGGGAAACACTTGATCTCATTAAAGAGTGCGACATTAACTTTAGCGTATTCTTTAGCATTAA
CTTTTTGGAAGACAAGCAAACCGTTAAAAAACTTGTTGACGCTGGTTGCATTATGGTGCTTGTAGGCTTTGAATCCATCA
AGCAAAGCACCCTTGAGGCGTACAACAAAGGGCATGTTAATTCAGCCGATAAATTTTCGCGTCTTATTGAGGAGTGCCGT
CAAGCAGGCTTAAACGTACAAGGCAATTTTCTTGTCAATCCCGCCCTTGACAGCTACGAGGATATGGATGATTTGGCACA
GTTTGTGAGCAAGAACAATATTTTCATGCCCATCTTCCAAATTATTACCCCCTACCCTGGAACAACCATGTACTGGGAGT
ACAAAAATAAAGGGTTGATAACCGATGAGGATTGGGAAAAATATAACGCCATGAATTTGGTGATTCGCTCCGAAAAATAT
GACCCGATAGAGTTTCAGCACAAGTTTATGACAACCTACTATAAGGCATATTCATGGAAAAATATTGTGAATCGCGTTCG
TGTTAACCCCTACAAATTGCTCAATTTGGTTACCAGCATGGCGTTTCGCAAAAACATTCGAGAGCAACTACAAACGTTCC
GACAAGATCATCACATAAAAAAATAA

Upstream 100 bases:

>100_bases
CATTTGATTTAAACCAGCACGTAAATAAAGCATCTCAAGAGGCATAGTTAGAAGCAACGAGGGCACAATAGCAGCAACCA
TTTTTTAATAACGCCGCAAC

Downstream 100 bases:

>100_bases
GCGGGGTGGCTCTTCTGCGCAATGATACCTCTCAAGCGGAACACTTCGATCCATTTTTCGTTCACGGCATAACTTTTACT
AACCTCTCAGCATTTTTTTG

Product: Elongator protein 3/MiaB/NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MGNSVTQNGKSLNILLIAPKGKKDSKSNQKPLFNMAIGVLVSITPPQHHLEIIDEHFGDEINYDGKYDLVGITSRTIDAT
RAYEIADTFRAKGKKVILGGLHVSFNKEEARAHADCIVCGEAENLWSTLLDDAANNALKPDYDSKDFPSVTEIVPIDYAK
IAKVSKREKVDGTKSIPIYITRGCPFECSFCVTPNFTGKLYRAQKPDDLKRQIEEAKRVFFKANSKTAKPWFMLCDENLG
VSKKRLWETLDLIKECDINFSVFFSINFLEDKQTVKKLVDAGCIMVLVGFESIKQSTLEAYNKGHVNSADKFSRLIEECR
QAGLNVQGNFLVNPALDSYEDMDDLAQFVSKNNIFMPIFQIITPYPGTTMYWEYKNKGLITDEDWEKYNAMNLVIRSEKY
DPIEFQHKFMTTYYKAYSWKNIVNRVRVNPYKLLNLVTSMAFRKNIREQLQTFRQDHHIKK

Sequences:

>Translated_461_residues
MGNSVTQNGKSLNILLIAPKGKKDSKSNQKPLFNMAIGVLVSITPPQHHLEIIDEHFGDEINYDGKYDLVGITSRTIDAT
RAYEIADTFRAKGKKVILGGLHVSFNKEEARAHADCIVCGEAENLWSTLLDDAANNALKPDYDSKDFPSVTEIVPIDYAK
IAKVSKREKVDGTKSIPIYITRGCPFECSFCVTPNFTGKLYRAQKPDDLKRQIEEAKRVFFKANSKTAKPWFMLCDENLG
VSKKRLWETLDLIKECDINFSVFFSINFLEDKQTVKKLVDAGCIMVLVGFESIKQSTLEAYNKGHVNSADKFSRLIEECR
QAGLNVQGNFLVNPALDSYEDMDDLAQFVSKNNIFMPIFQIITPYPGTTMYWEYKNKGLITDEDWEKYNAMNLVIRSEKY
DPIEFQHKFMTTYYKAYSWKNIVNRVRVNPYKLLNLVTSMAFRKNIREQLQTFRQDHHIKK
>Mature_460_residues
GNSVTQNGKSLNILLIAPKGKKDSKSNQKPLFNMAIGVLVSITPPQHHLEIIDEHFGDEINYDGKYDLVGITSRTIDATR
AYEIADTFRAKGKKVILGGLHVSFNKEEARAHADCIVCGEAENLWSTLLDDAANNALKPDYDSKDFPSVTEIVPIDYAKI
AKVSKREKVDGTKSIPIYITRGCPFECSFCVTPNFTGKLYRAQKPDDLKRQIEEAKRVFFKANSKTAKPWFMLCDENLGV
SKKRLWETLDLIKECDINFSVFFSINFLEDKQTVKKLVDAGCIMVLVGFESIKQSTLEAYNKGHVNSADKFSRLIEECRQ
AGLNVQGNFLVNPALDSYEDMDDLAQFVSKNNIFMPIFQIITPYPGTTMYWEYKNKGLITDEDWEKYNAMNLVIRSEKYD
PIEFQHKFMTTYYKAYSWKNIVNRVRVNPYKLLNLVTSMAFRKNIREQLQTFRQDHHIKK

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52843; Mature: 52712

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNSVTQNGKSLNILLIAPKGKKDSKSNQKPLFNMAIGVLVSITPPQHHLEIIDEHFGDE
CCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHCCCC
INYDGKYDLVGITSRTIDATRAYEIADTFRAKGKKVILGGLHVSFNKEEARAHADCIVCG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHCCCCEEEEC
EAENLWSTLLDDAANNALKPDYDSKDFPSVTEIVPIDYAKIAKVSKREKVDGTKSIPIYI
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCEEE
TRGCPFECSFCVTPNFTGKLYRAQKPDDLKRQIEEAKRVFFKANSKTAKPWFMLCDENLG
ECCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCC
VSKKRLWETLDLIKECDINFSVFFSINFLEDKQTVKKLVDAGCIMVLVGFESIKQSTLEA
CCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCEEEEECHHHHHHHHHHH
YNKGHVNSADKFSRLIEECRQAGLNVQGNFLVNPALDSYEDMDDLAQFVSKNNIFMPIFQ
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEHHH
IITPYPGTTMYWEYKNKGLITDEDWEKYNAMNLVIRSEKYDPIEFQHKFMTTYYKAYSWK
HHCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
NIVNRVRVNPYKLLNLVTSMAFRKNIREQLQTFRQDHHIKK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GNSVTQNGKSLNILLIAPKGKKDSKSNQKPLFNMAIGVLVSITPPQHHLEIIDEHFGDE
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHHHHCCCC
INYDGKYDLVGITSRTIDATRAYEIADTFRAKGKKVILGGLHVSFNKEEARAHADCIVCG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEECHHHHHCCCCEEEEC
EAENLWSTLLDDAANNALKPDYDSKDFPSVTEIVPIDYAKIAKVSKREKVDGTKSIPIYI
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHHHHHCCCCCCCCEEE
TRGCPFECSFCVTPNFTGKLYRAQKPDDLKRQIEEAKRVFFKANSKTAKPWFMLCDENLG
ECCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCC
VSKKRLWETLDLIKECDINFSVFFSINFLEDKQTVKKLVDAGCIMVLVGFESIKQSTLEA
CCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCEEEEECHHHHHHHHHHH
YNKGHVNSADKFSRLIEECRQAGLNVQGNFLVNPALDSYEDMDDLAQFVSKNNIFMPIFQ
HCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEEHHH
IITPYPGTTMYWEYKNKGLITDEDWEKYNAMNLVIRSEKYDPIEFQHKFMTTYYKAYSWK
HHCCCCCCEEEEEECCCCCCCCCCHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
NIVNRVRVNPYKLLNLVTSMAFRKNIREQLQTFRQDHHIKK
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]