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The map label for this gene is topB [H]

Identifier: 78067950

GI number: 78067950

Start: 3648872

End: 3651469

Strand: Direct

Name: topB [H]

Synonym: Bcep18194_A6481

Alternate gene names: 78067950

Gene position: 3648872-3651469 (Clockwise)

Preceding gene: 78067949

Following gene: 78067952

Centisome position: 98.77

GC content: 63.9

Gene sequence:

>2598_bases
ATGTCCAAAGCACTGATCATTGCGGAAAAGCCTTCTGTCGCGAACGACATCGCACGCGCTTTGGGCGGCTTTACCAAGCA
TGACGAGTATTACGAAAGCGACGAGTTCGTCCTTTCGTCCGCTGTCGGCCACCTGCTGGAAATCGCCGCCCCGGAAGAGT
ACGAGGTCAAGCGCGGGAAATGGAGCTTCGCGCATCTGCCCGTCATCCCCCCGCATTTCGACCTGAACCCGATCGCAAAA
AGCGAGTCGCGCCTCAAGGTGCTCACGAAGCTGTTGAAGCGCAAGGACGTCGACCGCCTGATCAACGCATGTGACGCGGG
GCGCGAGGGCGAGCTGATTTTCCGCCTGATCGCGCAGCACGCGAAGGCGAAGCAGCCGGTCCAGCGCCTGTGGCTGCAGT
CGATGACCCCGCAGGCGATCCGCGACGGCTTCGCGAACCTGCGCAGCGACGCGGACATGCTGCCGCTCGCCGACGCCGCA
CGCTGCCGCTCGGAAGCCGACTGGCTCGTCGGGATCAACGGCACCCGCGCGATGACCGCGTTCAACAGCAAGGGTGGCGG
CTTCTTCCTGACGACAGTTGGCCGGGTTCAGACGCCAACGTTGTCGATCGTCGTCGAACGCGAAGAGAAAATCCGCCGCT
TCATCCCGCGCGACTACTGGGAAGTGAAGGCCGAATTCGCGTGCGCGGGCGGCTTCTACGAAGGCAAGTGGTACGACCCG
AAATTCAAGCGCGACGAATTCGATCCGGAAAAGCGCGACTCCCGCCTGTGGAGCCTGCCGGCTGCCGAAACGATCGTCGC
CGCGTGTCGCGACCAGGTCGGCACGGTCTCCGAGGAATCGAAGCCGTCGACGCAGCTGTCGCCGCTGCTGTTCGACCTGA
CGAGCCTGCAGCGTGAAGCGAATAGCCGCTTCGGCTTCTCCGCGAAGAACACGCTCGGCCTCGCGCAGGCGCTGTATGAA
AAGCACAAGGTGCTGACCTACCCGCGTACCGACGCGCGCGCACTGCCGGAAGACTACCTGTCGACGGTCCAGTCCACGCT
CGAGATGCTCAAGGAGAGCAACAACTACCTGCCGCATGCGAAGCAGGTGCTCGACAAGGGCTGGGTGAAGCCGAACAAGC
GGATCTTCGACAACTCGAAGATCAGCGACCACTTTGCAATCATCCCGACGCTGCAGGCGCCGAAGTCGCTGTCCGAGCCG
GAGCAGAAGCTGTACGACATGGTCGTGAAGCGCTTCCTCGCCGTGTTCTTCCCGGCCGCCGAATTCCGCGTCACGACGCG
AATCACCGAAGTCGCCGGCCATCACTTCAAGACCGAAGGCAAGGTGCTCGTCGAGCCGGGCTGGCTGCAGGTGTACGGCC
GCGACGCCGAAGGCGCGGATGCGAACCTGGTGCCGGTGCAGAAGGACGAGAAGGTGAAGACGGACGAAATCGCCGCCGTC
GCGCTCGTCACGAAGCCGCCCGCACGCTACTCGGAAGCGACGCTGCTGTCCGCGATGGAAGGCGCGGGCAAGCTCGTCGA
GGACGACGAACTGCGCGAGGCGATGGCCGCAAAGGGCCTCGGTACGCCGGCCACGCGCGCGGCGATCATCGAAGGCCTGC
TCGGCGAGAAATACCTCGTGCGCGAAGGCCGCGAGCTGATCCCGACCGCGAAGGCATTCCAGCTGATGACGCTGCTGCGC
GGGCTCGGCGTGAAGGAACTCACCGCGCCCGAGCTCACCGGCGAATGGGAATACAAGCTGTCGCAGATGGAGCGCGGCAA
CCTAGGGCGCGAAGCGTTCATGCAGGAAATTGCCCGCATGACGCAGCAGATCGTCAAGCGCGCGAAGGAATACGATTCCG
ACACGATCCCCGGCGATTACGCGACGCTCGAGACGCCGTGCCCGAACTGCGGCGGCCAGGTGAAGGAAAACTACCGCCGT
TTCGCGTGCACGAAGTGCGAGTTCTCGATCTCGAAGATTCCGGGCAGCCGGCAGTTCGAGATCGCGGAAGTCGAGGAGCT
GCTGCGCGAGAAGACGATCGGCCCGCTGTCCGGCTTCCGCAGCAAGATGGGCCGCCCGTTCTCGGCGATCCTCAAGCTGT
CCTTCGACGACGAGACGAAGAACTACAAGCTCGAGTTCGATTTCGGCCAGGATTCCGGTGGTGAAGAAGGCGAGGCGCCC
GACTTCTCCGCGCAGGAGCCGGTCGGCGCATGCCCGAAGTGCAAGGGCCGCGTGTTCGAGCACGGGATGAGCTACGTGTG
CGAGCACTCGGTGGCCAACCCGAAGACCTGCGACTTCCGCTCAGGCAAGGTGATTCTGCAGCAGGAAATCACGCGCGAGC
AGATGGGCAAGCTGCTTGCCGACGGCCGCACGGATCTGCTGCCGAACTTCAAGTCGTCACGCACGGGCCGCAACTTCAAG
GCCTTCCTCGTGAAGCAGCCCGACGGCAAGATCGGCTTCGAGTTCGAGAAGAAGGAGCCGAAGGCCGCTGCCGCGAAGAA
GACGGCAAAATCGGCGACGAAGGATGCCGAAACCGTGACGGAAGGCGCCGAAGAGAAACCGGCACCGGCTCGCAAGACCG
CCGCCCGCAAGACGACGGCGCGCAAGACGGGCTCGTAA

Upstream 100 bases:

>100_bases
CTTTTTACACGCTGAGCCGCCATCGCTGCGGCGTGTGCTATAAAGCGGTCGTAAAAGGGACCCACAACCGGCGAACCCGG
TCTACCAACACACACCTGTC

Downstream 100 bases:

>100_bases
CGGCCTGACGGCCGGGGTTCTCCCGGCCACGCCGGATTCCGGCAGGCAGCGATAAAAAAACGCGGATCGATTGCTCGATC
CGCGTTTTTCTTTGTGCCGG

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDADMLPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACAGGFYEGKWYDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGREAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCEFSISKIPGSRQFEIAEVEELLREKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDSGGEEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS

Sequences:

>Translated_865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDADMLPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACAGGFYEGKWYDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEP
EQKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGREAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCEFSISKIPGSRQFEIAEVEELLREKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDSGGEEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS
>Mature_864_residues
SKALIIAEKPSVANDIARALGGFTKHDEYYESDEFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAKS
ESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDADMLPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACAGGFYEGKWYDPK
FKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREANSRFGFSAKNTLGLAQALYEK
HKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPE
QKLYDMVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAVA
LVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLVREGRELIPTAKAFQLMTLLRG
LGVKELTAPELTGEWEYKLSQMERGNLGREAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRF
ACTKCEFSISKIPGSRQFEIAEVEELLREKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDSGGEEGEAPD
FSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFKA
FLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=647, Percent_Identity=25.8114374034003, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI4507635, Length=656, Percent_Identity=24.2378048780488, Blast_Score=120, Evalue=6e-27,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=34.248788368336, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1787529, Length=665, Percent_Identity=25.7142857142857, Blast_Score=152, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=626, Percent_Identity=25.8785942492013, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=23.8170347003155, Blast_Score=105, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323263, Length=663, Percent_Identity=25.6410256410256, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24585251, Length=652, Percent_Identity=25.3067484662577, Blast_Score=113, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24640096, Length=640, Percent_Identity=22.5, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96400; Mature: 96269

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDEFVLSSAVGHLLEIAAPEEYEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFANLRSDADMLPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACAGGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGREAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCEFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCCEE
IAEVEELLREKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDSGGEEGEAP
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGAEEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKALIIAEKPSVANDIARALGGFTKHDEYYESDEFVLSSAVGHLLEIAAPEEYEVKRGK
CCEEEEECCCCHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLLKRKDVDRLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFANLRSDADMLPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACAGGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHEEECCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQVGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NSRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKSLSEPEQKLYDMVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLV
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGREAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCEFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCEEHHHCCCCCCEE
IAEVEELLREKTIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDSGGEEGEAP
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGAEEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA