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The map label for this gene is cca

Identifier: 78067847

GI number: 78067847

Start: 3544918

End: 3546159

Strand: Reverse

Name: cca

Synonym: Bcep18194_A6378

Alternate gene names: 78067847

Gene position: 3546159-3544918 (Counterclockwise)

Preceding gene: 78067848

Following gene: 78067845

Centisome position: 95.99

GC content: 70.45

Gene sequence:

>1242_bases
ATGAATATCTACGCAGTAGGCGGCGCGATCCGCGACGAATTGCTCGGCGTGCCCGTGCAGGACCGCGACTACGTGGTGGT
GGGCGCGACCCCCGAGCAGATGGCCGCGCAGGGTTTCCGGCCGGTCGGCAAGGATTTCCCGGTGTTCCTGCATCCGCGCA
CGCAGGAGGAGTACGCGCTGGCGCGCACCGAGCGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCCATTACGCGCCG
GACGTGACGCTCGACGAGGATCTCGCGCGGCGCGACCTGACGGTCAACGCGATGGCGCGCGAGGTGAGCCCGGAGGGCGA
GCTGGTCGGGCCGGTGATCGATCCGTTCGACGGGCAGGCCGATTTGCGCGCGCGCGTGTTCCGGCATGTGAGCGATGCGT
TCGTCGAGGACCCGGTGCGGATCCTGCGTATCGCGCGCTTCGCCGCGCGGTTCGCGGATTTCACGGTCGCGGACGAAACG
CTCGCGCTGATGCGGCGGATGGTCGACGCGGGCGAGGTCGACGCGCTCGTGCCCGAGCGCGTGTGGCAGGAAATCGCGCG
CGGGCTGATGGAAGCGAAGCCGTCGCGGATGTTCGCGGTGCTGCGCGAGTGCGGTGCGCTCGCGCGCATCCTGCCGGAAG
TCGATGCGTTGTGGGGCGTGCCGCAGCGCGCCGACTACCACCCGGAGGTCGACACGGGCGTGCACGTGATGATGGTCGTC
GACTATGCGGCGAAGCAGGGGTATTCGCTGCCGGTGCGCTTCGCGGCGCTCACGCACGATCTCGGCAAGGCGACGACGCC
GGCCGACGTGCTGCCGCGCCACGTCGGCCACGAGGGGCGCAGCGTCGACCTGCTCAAGCCGTTGTGCGAGCGGTTGCGCG
TGCCGAACGAATGCCGCGATCTCGCGCTGGTGGTCGCGCGCGAGCACGGCAACCTGCATCGCGTGATGGAGATGGGCGCG
GCCGCGCTGGTGCGGCTGTTCGAGCGCAGCGACGCGCTGCGCAAGCCGGCGCGTTTCGCCGAGATGCTGCAGGCGTGCGA
ATCGGATGCGCGCGGGCGGCTTGGGCTCGACGCGCAGCCGTATCCGCAGGCCGAGCGGCTGCGCGTCGCGCTCGCGGCCG
CGCGCAGCGTCGATGCCGGTGCAATCGCGCGCGGCATCGGCAACGATACGGAGAAGATCAAGGAAGCCGTGCATCGCGCA
CGGATCGAGGCCGTCGCGCAGGCGCTCGAGATCGGCGAATAG

Upstream 100 bases:

>100_bases
CCGGCTATGCGGCCCGCGTGACGGCGCTGCCGGCCGTGCAGCGCTGGATCGACGCCGCGCGCCGCGAAACGAACGTGATC
GCCGAGTACGAGCCGACGCC

Downstream 100 bases:

>100_bases
GGGGAAGGGCGCGCATCCCGCGCCTGCCTCCCGATAACCGTTACGACTTCTTCTTGCGCGCCGGCTTGGCGGCCGCCGGA
CGTTTGCCGGCGGATGCGCG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMAAQGFRPVGKDFPVFLHPRTQEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTVNAMAREVSPEGELVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET
LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRECGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV
DYAAKQGYSLPVRFAALTHDLGKATTPADVLPRHVGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLFERSDALRKPARFAEMLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA
RIEAVAQALEIGE

Sequences:

>Translated_413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMAAQGFRPVGKDFPVFLHPRTQEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTVNAMAREVSPEGELVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET
LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRECGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV
DYAAKQGYSLPVRFAALTHDLGKATTPADVLPRHVGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLFERSDALRKPARFAEMLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA
RIEAVAQALEIGE
>Mature_413_residues
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMAAQGFRPVGKDFPVFLHPRTQEEYALARTERKTAAGYHGFQFHYAP
DVTLDEDLARRDLTVNAMAREVSPEGELVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET
LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRECGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV
DYAAKQGYSLPVRFAALTHDLGKATTPADVLPRHVGHEGRSVDLLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA
AALVRLFERSDALRKPARFAEMLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA
RIEAVAQALEIGE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=52.1844660194175, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45645; Mature: 45645

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMAAQGFRPVGKDFPVFLHPRTQEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTVNAMAREVSPEGELVGPVIDPFDGQA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH
DLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADETLALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH
VWQEIARGLMEAKPSRMFAVLRECGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHEE
DYAAKQGYSLPVRFAALTHDLGKATTPADVLPRHVGHEGRSVDLLKPLCERLRVPNECRD
EHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRLFERSDALRKPARFAEMLQACESDARGRLGLDAQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC
YPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRARIEAVAQALEIGE
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMAAQGFRPVGKDFPVFLHPRTQEEYAL
CEEEEECCHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTVNAMAREVSPEGELVGPVIDPFDGQA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH
DLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADETLALMRRMVDAGEVDALVPER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH
VWQEIARGLMEAKPSRMFAVLRECGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHEE
DYAAKQGYSLPVRFAALTHDLGKATTPADVLPRHVGHEGRSVDLLKPLCERLRVPNECRD
EHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
LALVVAREHGNLHRVMEMGAAALVRLFERSDALRKPARFAEMLQACESDARGRLGLDAQP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC
YPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRARIEAVAQALEIGE
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA