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The map label for this gene is flgJ

Identifier: 78067832

GI number: 78067832

Start: 3530287

End: 3531270

Strand: Reverse

Name: flgJ

Synonym: Bcep18194_A6363

Alternate gene names: 78067832

Gene position: 3531270-3530287 (Counterclockwise)

Preceding gene: 78067833

Following gene: 78067831

Centisome position: 95.59

GC content: 69.51

Gene sequence:

>984_bases
ATGGCAGCCAGTCTCCCGAACGCGAACGACCTCACGCAGCGCTTTGCGCTCGACGTGCAGGGTTTCGACGCCCTGCGCGC
GCAGGCGAAGCAGTCGCCGCAGGCCGGCGCGAAGGCGGTCGCCGGCCAGTTCGACGCGATGTTCACGCAGATGATGCTCA
AGAGCATGCGCGACGCGTCGCCCGACGGCGGGCTGTTCGATTCGCATACGTCGAAGATGTACACGTCGATGCTCGACCAG
CAGCTCGCGCAGCAGATGTCGACGCGCGGGATCGGTGTCGCCGACGCGCTGATGAAGCAGCTGCTGCGCAATGCGGGGCA
GGGCGCGGGCAGCGACACGGCGGCCGACGTCGGCGCGGCCGGCCTGGGCGCGGACGGCTTCGGCGCGTCCGGTAACGAAG
GTGGCCTCGCCGCGATGAACGCGATGGCGAAGGCCTACGCGAACTCGGCCAACAACGGCGGGCTCGCCGGCACGCGCGGC
TACTCGGCCGGCAGCGCGCTGACGCCGCCGCTGAAGGGCGCGAGCGGCGTGCAGGACGCCGACGCGTTCGTCGACCGGCT
CGCGGGCCCCGCGCAGGCGGCTAGCGCGTCGACCGGCATTCCGGCGCGCTTCATCGTCGGCCAGGCCGCGCTCGAATCGG
GCTGGGGCAAGCGCGAGATCCGCGCGGGCGACGGTTCGACGAGCTACAACGTGTTCGGCATCAAGGCGACCAAGGGCTGG
ACCGGTCGCACGGTGTCGGCGCTGACCACGGAGTACGTGAACGGCACGCCGCGCCGCGTGGTCGCGAAATTCCGTGCGTA
CGATTCGTACGAGCACGCGATGACCGATTACGCGAACCTGCTGAAGAACAACCCGCGCTACTCGGGCGTGCTGAGCGCGA
GCCGCAGCGTCGAAGGCTTCGCGCACGGGATGCAGAAGGCCGGCTACGCGACCGACCCGAACTACGCGAAGAAGCTGATT
TCGATCATGCAGCAGATCGGCTGA

Upstream 100 bases:

>100_bases
GGCGCTGAACACGCTCGGCGCAACGCCCGCGGACCTGATGTCGATCCTGCAGGCGATGAAGGCCGCCGGCGCGCTGCGCG
CCGACCTGGAGATCATCTAA

Downstream 100 bases:

>100_bases
CCGGCCCGCCGGCGACCGCCGTTGTACCCTCTGGACCGCGCCCGGCAGCCCGCCGCGCGCGGTTTCAACGTTTGCGCGAA
AAAAATCCCCAATTCGATCT

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAASLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQ
QLAQQMSTRGIGVADALMKQLLRNAGQGAGSDTAADVGAAGLGADGFGASGNEGGLAAMNAMAKAYANSANNGGLAGTRG
YSAGSALTPPLKGASGVQDADAFVDRLAGPAQAASASTGIPARFIVGQAALESGWGKREIRAGDGSTSYNVFGIKATKGW
TGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYSGVLSASRSVEGFAHGMQKAGYATDPNYAKKLI
SIMQQIG

Sequences:

>Translated_327_residues
MAASLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQ
QLAQQMSTRGIGVADALMKQLLRNAGQGAGSDTAADVGAAGLGADGFGASGNEGGLAAMNAMAKAYANSANNGGLAGTRG
YSAGSALTPPLKGASGVQDADAFVDRLAGPAQAASASTGIPARFIVGQAALESGWGKREIRAGDGSTSYNVFGIKATKGW
TGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYSGVLSASRSVEGFAHGMQKAGYATDPNYAKKLI
SIMQQIG
>Mature_326_residues
AASLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDASPDGGLFDSHTSKMYTSMLDQQ
LAQQMSTRGIGVADALMKQLLRNAGQGAGSDTAADVGAAGLGADGFGASGNEGGLAAMNAMAKAYANSANNGGLAGTRGY
SAGSALTPPLKGASGVQDADAFVDRLAGPAQAASASTGIPARFIVGQAALESGWGKREIRAGDGSTSYNVFGIKATKGWT
GRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYSGVLSASRSVEGFAHGMQKAGYATDPNYAKKLIS
IMQQIG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=312, Percent_Identity=40.3846153846154, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33809; Mature: 33678

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDAS
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDGGLFDSHTSKMYTSMLDQQLAQQMSTRGIGVADALMKQLLRNAGQGAGSDTAADVGAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCC
GLGADGFGASGNEGGLAAMNAMAKAYANSANNGGLAGTRGYSAGSALTPPLKGASGVQDA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DAFVDRLAGPAQAASASTGIPARFIVGQAALESGWGKREIRAGDGSTSYNVFGIKATKGW
HHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCCC
TGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYSGVLSASRSVEGF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHGMQKAGYATDPNYAKKLISIMQQIG
HHHHHHCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AASLPNANDLTQRFALDVQGFDALRAQAKQSPQAGAKAVAGQFDAMFTQMMLKSMRDAS
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDGGLFDSHTSKMYTSMLDQQLAQQMSTRGIGVADALMKQLLRNAGQGAGSDTAADVGAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCC
GLGADGFGASGNEGGLAAMNAMAKAYANSANNGGLAGTRGYSAGSALTPPLKGASGVQDA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
DAFVDRLAGPAQAASASTGIPARFIVGQAALESGWGKREIRAGDGSTSYNVFGIKATKGW
HHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEECCCC
TGRTVSALTTEYVNGTPRRVVAKFRAYDSYEHAMTDYANLLKNNPRYSGVLSASRSVEGF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHGMQKAGYATDPNYAKKLISIMQQIG
HHHHHHCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]