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The map label for this gene is gcvA [H]

Identifier: 78067827

GI number: 78067827

Start: 3523327

End: 3524211

Strand: Direct

Name: gcvA [H]

Synonym: Bcep18194_A6358

Alternate gene names: 78067827

Gene position: 3523327-3524211 (Clockwise)

Preceding gene: 78067820

Following gene: 161702954

Centisome position: 95.38

GC content: 69.38

Gene sequence:

>885_bases
ATGGCACGCGATCTCCCACCCTTTTCCGCGCTTCGGGCCTTTGAAGCCGCGGCACGACATGAAAGCTTCAGCGCCGCCGG
CGACGAGCTGCATGTGACTCACGGTGCCATCAGCCGGCAGATCGCCGGGTTCGAGGCCTGGCTCGGCAAGCCCGTGTTCC
ACCGCTATGGCAAGCGCGTGAAGCTCACCGACGAAGGCCGACGCTACCTGGACACCGTGCGCGCGGCATTCGACAGCATC
GCGCTCGCGACCGAGCAGCTGCGCAACACGGGCGCGGCACGCGTGCTGCGCATCAATGCGCTGCCGACCTTCGCGATGAA
ATGGCTGCTGCCGCGCCTGTCGCGGTTCCAGCGTGACGTGCCGAACGTCGAGCTGAAGCTGTCGACGTCGAATGCGCCGC
TCGACACGCTCGACGGCTTCGATGTCGCGATCCGCCGTGGCCCCGGCCACTGGCCGAACTGCACGAGCGGCCACTTCCTC
GACGAAAGCGTGATCCCCGTCTGCAGCCCCGCGCTGCTCAAGCGCATGCCGATCGCGCGCGCGGACGATCTCGCGCGCCA
TGTGCTGCTGCATTCGGATACGCGGCCGGAAGGCTGGCGCGACTGGTTCGCGGCGGCCGGCGTGACGATGAAGGGGCGCA
AGCGGCAGTCGTTCGACCACTTCTACCTGGCACTGCAGGCGGCCGTCGACGGCCTGGGCGTCGCGCTCGGCCCGTTGCCG
CTGATCGACGATGAACTCGCGAGCGGCCGGCTGGTGATGCCGCTGGAGGGGCCGCGCATCGCGACGCGCAGCTACTGGTG
GATCGCGCCGCGCGCGCCGGCCGACGATCCGCTCGTCGCGCAGTTCTGCGCGTGGCTGCAGACGCAGTCGAACGTCGGCG
CGTAG

Upstream 100 bases:

>100_bases
GCATGGGGTTCCTTATCAAACGGTGACCCGAGGTTACCGCCGTTCGGAATACCGGCAAAACGATTTTATCGGCGCGTCCT
TGTGATCTAGAATCACAAGC

Downstream 100 bases:

>100_bases
CGCCGCGACGTTCGACGCCGAAGATGACACGGGCGGCACGACACCGCCCGCCGGAAGCTCAGACCACCGGGCCCGGTTCC
TTTTCCTTACGCAGGATCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRMPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLEGPRIATRSYWWIAPRAPADDPLVAQFCAWLQTQSNVGA

Sequences:

>Translated_294_residues
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSI
ALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPNCTSGHFL
DESVIPVCSPALLKRMPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
LIDDELASGRLVMPLEGPRIATRSYWWIAPRAPADDPLVAQFCAWLQTQSNVGA
>Mature_293_residues
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRVKLTDEGRRYLDTVRAAFDSIA
LATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDVPNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPNCTSGHFLD
ESVIPVCSPALLKRMPIARADDLARHVLLHSDTRPEGWRDWFAAAGVTMKGRKRQSFDHFYLALQAAVDGLGVALGPLPL
IDDELASGRLVMPLEGPRIATRSYWWIAPRAPADDPLVAQFCAWLQTQSNVGA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=35.6401384083045, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=29.6551724137931, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=30.1369863013699, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787128, Length=143, Percent_Identity=34.2657342657343, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786401, Length=262, Percent_Identity=27.4809160305344, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=30.2469135802469, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=26.2548262548263, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790011, Length=283, Percent_Identity=22.6148409893993, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32550; Mature: 32418

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRMPIAR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLEGPRIATRSYWWIAPRAPADDPLVAQFCAWLQTQSNVGA
CCCHHHCCCEEEEECCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARDLPPFSALRAFEAAARHESFSAAGDELHVTHGAISRQIAGFEAWLGKPVFHRYGKRV
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHCCCEE
KLTDEGRRYLDTVRAAFDSIALATEQLRNTGAARVLRINALPTFAMKWLLPRLSRFQRDV
EECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCC
PNVELKLSTSNAPLDTLDGFDVAIRRGPGHWPNCTSGHFLDESVIPVCSPALLKRMPIAR
CCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
ADDLARHVLLHSDTRPEGWRDWFAAAGVTMKGRKRQSFDHFYLALQAAVDGLGVALGPLP
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHCCCCC
LIDDELASGRLVMPLEGPRIATRSYWWIAPRAPADDPLVAQFCAWLQTQSNVGA
CCCHHHCCCEEEEECCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]