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The map label for this gene is gcvA [H]

Identifier: 78067773

GI number: 78067773

Start: 3469406

End: 3470326

Strand: Direct

Name: gcvA [H]

Synonym: Bcep18194_A6304

Alternate gene names: 78067773

Gene position: 3469406-3470326 (Clockwise)

Preceding gene: 78067771

Following gene: 78067774

Centisome position: 93.92

GC content: 72.31

Gene sequence:

>921_bases
ATGCGACGCATCCACCTTCCTCCGCTGCAGACGCTGCGCGCGTTCGAGACCGCCGTGCGGCTGCAGAGCTTCACGCGTGC
GGCCGACGAACTCGCGCTCACGCAAGGCGCCGTCAGCCAGCACATCCGCGCACTCGAGGCGCAGCTCGGCTATCCGCTCT
TCACGCGCGAGCGTAACGGCGCGACGCCGACGCACGCCGCGCACGCGCTCGCACTGCAGGTGCGCCAGGGCCTCAGCGTG
CTCGAGCGCGCGTTCGAGCCGGCACTCTCCGTACGCAGCCGCCCGCGCACCGGCGACGTCACGCTGAACGTCAGCGTGCT
GCCGAGCGTCGCCGAGCGCTGGCTTGCGCCGCGCCTGCCGCGCTTCTCGGCCGCGCATCCGCACATCGCGATCGCGCTGC
ACCCGGACGTGGCGCTTGCGCCGCTGCGCAAGCGCGACCGCATCGACGTCGCGCTGCGCTACGGGCCCGGCACGTGGCCG
GGCGTCGTCGCCGAGAAGCTGATGAACGAGACGATCTTTCCGGTGGCGAGCCCCACGTACCGCAATCGCGACGGCGTCGC
GCCGCGCGCGCCGGCGGATCTCGTGCGCGCGACGCTGTTGCGCCATCCCGCGCAACCGTGGGAACCGTGGCTCCAGGCCG
CGCGGCTCGACCTCACGGAATCCGCACGCGCGCCGCGTTTCACCGATGCAAATGCGCTGATCGACGCGACGCTGAAGGGG
CGCGGCGTCGCACTCGCGCGCCGCTCGCTGATCGAGCCCGAACTCGCGAACGGCACGCTCGTGCGCGTATCGACGGTGCG
CGTGACCGACGTGTATGCGCACTACGTCGTGTGGCGCCCGGGCCATCCACATGAAGCGGCGATCCGCACCTGGCTGGACT
GGCTGCGCAGCGAGGTGCGGCGACGCACACCGCGTCGCTGA

Upstream 100 bases:

>100_bases
CGACGGGGCTGTGGAAGACGGAACCGAGTGTAGGAACGCGTGCATGCCCGCCGCAAACGATGAATTGTCGTGCTCGGGCA
TTAGTGTTACTAATACCCGC

Downstream 100 bases:

>100_bases
GGCCACGCGATCGGCCGCACACGTCCATCATGAACGGTCAAGCCACCGTGGACGCGCGCGTGCATTGCATCGCGCACCCG
GGTAAAAGTGCCCGGCCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRRIHLPPLQTLRAFETAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPALSVRSRPRTGDVTLNVSVLPSVAERWLAPRLPRFSAAHPHIAIALHPDVALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETIFPVASPTYRNRDGVAPRAPADLVRATLLRHPAQPWEPWLQAARLDLTESARAPRFTDANALIDATLKG
RGVALARRSLIEPELANGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR

Sequences:

>Translated_306_residues
MRRIHLPPLQTLRAFETAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPALSVRSRPRTGDVTLNVSVLPSVAERWLAPRLPRFSAAHPHIAIALHPDVALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETIFPVASPTYRNRDGVAPRAPADLVRATLLRHPAQPWEPWLQAARLDLTESARAPRFTDANALIDATLKG
RGVALARRSLIEPELANGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR
>Mature_306_residues
MRRIHLPPLQTLRAFETAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPALSVRSRPRTGDVTLNVSVLPSVAERWLAPRLPRFSAAHPHIAIALHPDVALAPLRKRDRIDVALRYGPGTWP
GVVAEKLMNETIFPVASPTYRNRDGVAPRAPADLVRATLLRHPAQPWEPWLQAARLDLTESARAPRFTDANALIDATLKG
RGVALARRSLIEPELANGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVRRRTPRR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=33.9041095890411, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=28.3828382838284, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=308, Percent_Identity=30.5194805194805, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693193, Length=207, Percent_Identity=33.8164251207729, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=305, Percent_Identity=24.9180327868852, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787589, Length=300, Percent_Identity=26, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34216; Mature: 34216

Theoretical pI: Translated: 11.90; Mature: 11.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIHLPPLQTLRAFETAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPTHAAHALALQVRQGLSVLERAFEPALSVRSRPRTGDVTLNVSVLPSVAERWLAPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCC
RFSAAHPHIAIALHPDVALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETIFPVASPTY
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGVAPRAPADLVRATLLRHPAQPWEPWLQAARLDLTESARAPRFTDANALIDATLKG
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHEEECCCC
RGVALARRSLIEPELANGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVR
CCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC
>Mature Secondary Structure
MRRIHLPPLQTLRAFETAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPTHAAHALALQVRQGLSVLERAFEPALSVRSRPRTGDVTLNVSVLPSVAERWLAPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCC
RFSAAHPHIAIALHPDVALAPLRKRDRIDVALRYGPGTWPGVVAEKLMNETIFPVASPTY
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGVAPRAPADLVRATLLRHPAQPWEPWLQAARLDLTESARAPRFTDANALIDATLKG
CCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCCCCCHHEEECCCC
RGVALARRSLIEPELANGTLVRVSTVRVTDVYAHYVVWRPGHPHEAAIRTWLDWLRSEVR
CCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]