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The map label for this gene is dat [H]

Identifier: 78067704

GI number: 78067704

Start: 3392318

End: 3393244

Strand: Reverse

Name: dat [H]

Synonym: Bcep18194_A6235

Alternate gene names: 78067704

Gene position: 3393244-3392318 (Counterclockwise)

Preceding gene: 78067705

Following gene: 78067703

Centisome position: 91.86

GC content: 65.37

Gene sequence:

>927_bases
ATGAGCCAAGCCGAATTTGAACCGATCGTCTACCTCAGCGTGTCGTCGCAGGAGGAAATGGTGCCGCTGTCGGAAGCCCG
TGTACCGGTGCTCGACCGCGGGTTCATCTTCGGCGACGGCGTGTATGAAGTCGTCCCCGTTTATGCGCACGACGGCGCGC
ACGTGCCGTTCCGGATCGAGCAGCATCTGGCCCGGCTCGAGCGCAGCCTGAAGAAGATCGGCATCGCGAGCCCGCACGAC
GACGCGGGCTGGCGTGCGCTGATCGAGCGCGTCGTCGCGGCGAATGCCGAAGGGCTCGGCGAAGGCAACGCGCTCGTCTA
CCTGCAGGTGACGCGCGGTGTCGCGAAGCGCGGCCACGCGTTCCCGGCGAACGCGGTGCCGACGGTGTTCGTGATGACGA
GCCCGCTGCGCCTGCCGTCCGCCGAAGAACGCGCGCAGGGTGTGCGCTGCGTGACGGCCGAGGATCGTCGCTGGCTGCAT
TGCGACATCAAGTCGATCTCGCTGCTCGGCAACGTGCTGATGGCGCAGCATGCGGCCGAACGCGATGCGTTCGAGACGCT
GCAGCTGCGCGACGGCAACCTGACGGAAGGTTCGTCGTCGAACGTGTGGATCGTGAAGAACGGCGAGCTGCTTGCGCCGC
CGCGCAGCAACAAGATCCTCGAGGGGATCCGCTACGCGCTGATCGAGGAGCTGGCCGACGAATGCAAGATTCCGTTCGTT
GCACGCGAGATCAGCGAAGTGGAGTTGCGCGCAGCCGACGAAATCATGATCACGTCGGCCACGAAGGAAGTGCTGCCCGT
CACGTCGCTCGACGATCTGCCCGTGCAGGGCGGCAAGCCGGGCCCCGTGTTCGCGGCGTTGTATGACGCGTACCAGCGCG
CGAAAGCGCGCGAGTTTGAACAGTTTGACCTAACCCGGAGCAAATGA

Upstream 100 bases:

>100_bases
CTTCGGGCGTATCTGGGACTCGATCCTGCTGATGTTCAGCAAGAAGAAGTAAGCACGACGGGCCTTTGTTTTTACGACTT
CATCCCCATCTGTCATAGCT

Downstream 100 bases:

>100_bases
TGAGCGAACCGACCAAAACCATCGAGGTGACCGGCGCGATCGATACCCGGAAGGAGTCGCTGCTGGAGTTCCCGTGCGAT
TTCCCGATCAAGATCATGGG

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSQAEFEPIVYLSVSSQEEMVPLSEARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIEQHLARLERSLKKIGIASPHD
DAGWRALIERVVAANAEGLGEGNALVYLQVTRGVAKRGHAFPANAVPTVFVMTSPLRLPSAEERAQGVRCVTAEDRRWLH
CDIKSISLLGNVLMAQHAAERDAFETLQLRDGNLTEGSSSNVWIVKNGELLAPPRSNKILEGIRYALIEELADECKIPFV
AREISEVELRAADEIMITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLTRSK

Sequences:

>Translated_308_residues
MSQAEFEPIVYLSVSSQEEMVPLSEARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIEQHLARLERSLKKIGIASPHD
DAGWRALIERVVAANAEGLGEGNALVYLQVTRGVAKRGHAFPANAVPTVFVMTSPLRLPSAEERAQGVRCVTAEDRRWLH
CDIKSISLLGNVLMAQHAAERDAFETLQLRDGNLTEGSSSNVWIVKNGELLAPPRSNKILEGIRYALIEELADECKIPFV
AREISEVELRAADEIMITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLTRSK
>Mature_307_residues
SQAEFEPIVYLSVSSQEEMVPLSEARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIEQHLARLERSLKKIGIASPHDD
AGWRALIERVVAANAEGLGEGNALVYLQVTRGVAKRGHAFPANAVPTVFVMTSPLRLPSAEERAQGVRCVTAEDRRWLHC
DIKSISLLGNVLMAQHAAERDAFETLQLRDGNLTEGSSSNVWIVKNGELLAPPRSNKILEGIRYALIEELADECKIPFVA
REISEVELRAADEIMITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFEQFDLTRSK

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=283, Percent_Identity=25.7950530035336, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33956; Mature: 33825

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAEFEPIVYLSVSSQEEMVPLSEARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIE
CCCCCCCCEEEEEECCCCCCCCHHHCCCCEEECCEEECCCCEEEEEEEECCCCCCCHHHH
QHLARLERSLKKIGIASPHDDAGWRALIERVVAANAEGLGEGNALVYLQVTRGVAKRGHA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHCCCC
FPANAVPTVFVMTSPLRLPSAEERAQGVRCVTAEDRRWLHCDIKSISLLGNVLMAQHAAE
CCCCCCCEEEEEECCCCCCCHHHHHCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHH
RDAFETLQLRDGNLTEGSSSNVWIVKNGELLAPPRSNKILEGIRYALIEELADECKIPFV
HHHHHEEEECCCCCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AREISEVELRAADEIMITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFE
HHHHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFDLTRSK
HHCCCCCC
>Mature Secondary Structure 
SQAEFEPIVYLSVSSQEEMVPLSEARVPVLDRGFIFGDGVYEVVPVYAHDGAHVPFRIE
CCCCCCCEEEEEECCCCCCCCHHHCCCCEEECCEEECCCCEEEEEEEECCCCCCCHHHH
QHLARLERSLKKIGIASPHDDAGWRALIERVVAANAEGLGEGNALVYLQVTRGVAKRGHA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHCCCC
FPANAVPTVFVMTSPLRLPSAEERAQGVRCVTAEDRRWLHCDIKSISLLGNVLMAQHAAE
CCCCCCCEEEEEECCCCCCCHHHHHCCEEEEEECCCEEEEECHHHHHHHHHHHHHHHHHH
RDAFETLQLRDGNLTEGSSSNVWIVKNGELLAPPRSNKILEGIRYALIEELADECKIPFV
HHHHHEEEECCCCCCCCCCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHH
AREISEVELRAADEIMITSATKEVLPVTSLDDLPVQGGKPGPVFAALYDAYQRAKAREFE
HHHHHHHHHHCCCEEEEEECCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QFDLTRSK
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]