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The map label for this gene is lipB

Identifier: 78067700

GI number: 78067700

Start: 3389768

End: 3390526

Strand: Reverse

Name: lipB

Synonym: Bcep18194_A6231

Alternate gene names: 78067700

Gene position: 3390526-3389768 (Counterclockwise)

Preceding gene: 78067701

Following gene: 78067699

Centisome position: 91.78

GC content: 67.46

Gene sequence:

>759_bases
ATGTCCGTTTCGCCGGTTTCCATCGTGTCTACGCCTGTTGCCGTTTCCGCATCGCCCGCCGGGTCCCCGGATCAGCCGGC
CCCGCCGGTCACCGTGCGCTGGCGGGGTGTCGAGACCTACGAGGCGAGCTTCGACGCGATGCGCGCGTTCACCGACGCGC
GCACGGCCGACACCGGCGACGAGATCTGGCTGGTCGAGCACCCGCCCGTCTACACGCTCGGCCAGGCCGGCGACCCGTCC
CACCTGCTGGTGGCCGACAGCGGCGTGCCGCTCGTGAAGGTCGACCGCGGGGGGCAAATCACTTATCACGGCCCCGGCCA
GATCGTCGCGTACCTGCTGCTGGACCTGCGCCGGCGCAAGCTGATGGTGCGCACGCTGGTGACGAAGATCGAGGAGGCCG
TGATCGAAACCCTCGCGGCGTATAATCTCGCTTCGGTCCGCAAGGCGGGTGCGCCCGGGATCTACGTGGCATCCGGCGTG
CACGAGGGCGCGAAAATCGCGGCCCTCGGCCTGAAGATCCGCAACGGCTGCAGCTATCACGGGCTGAGCCTGAACGTGAA
GATGGATCTGCGCCCGTTTCTTGCGATCAACCCGTGCGGCTATGCCGGACTGGAAACTGTCGACATGGCGAGCCTCGAGG
TTGCCGCCGACTGGAACGACGTCGCCCACACGCTGGTGCGCCGTCTGATCGCCAACCTCGACGGCGCATCCGCGGCCGCC
GACAAGCCGCAGGCACTGGAACAACATTCGAATGACTGA

Upstream 100 bases:

>100_bases
ATGGCGCACCGTCTGACCACTATTTCCTCCGACGGATACCGGCGATCCGTCCCCTCTCCAAGGCCGGCGAGACCCACGGG
TTTTCGGTAAACTACCGCCC

Downstream 100 bases:

>100_bases
CGTTACCGCTTCCCCCGCACTGGCCGATTCGGCCGCGACCCCTGCCTACGATCCGACCGCCAAGCAGAAGGCGCAGGCGA
AGACGGCGCGCATCCCGATC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSVSPVSIVSTPVAVSASPAGSPDQPAPPVTVRWRGVETYEASFDAMRAFTDARTADTGDEIWLVEHPPVYTLGQAGDPS
HLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGV
HEGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVRRLIANLDGASAAA
DKPQALEQHSND

Sequences:

>Translated_252_residues
MSVSPVSIVSTPVAVSASPAGSPDQPAPPVTVRWRGVETYEASFDAMRAFTDARTADTGDEIWLVEHPPVYTLGQAGDPS
HLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGV
HEGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVRRLIANLDGASAAA
DKPQALEQHSND
>Mature_251_residues
SVSPVSIVSTPVAVSASPAGSPDQPAPPVTVRWRGVETYEASFDAMRAFTDARTADTGDEIWLVEHPPVYTLGQAGDPSH
LLVADSGVPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVH
EGAKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVRRLIANLDGASAAAD
KPQALEQHSND

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Escherichia coli, GI87081767, Length=205, Percent_Identity=50.7317073170732, Blast_Score=209, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323268, Length=163, Percent_Identity=30.6748466257669, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=187, Percent_Identity=32.620320855615, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BURS3 (Q39CJ1)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_370469.1
- ProteinModelPortal:   Q39CJ1
- SMR:   Q39CJ1
- STRING:   Q39CJ1
- GeneID:   3751464
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A6231
- NMPDR:   fig|269483.3.peg.7712
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- ProtClustDB:   PRK14343
- BioCyc:   BSP36773:BCEP18194_A6231-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26745; Mature: 26614

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPVSIVSTPVAVSASPAGSPDQPAPPVTVRWRGVETYEASFDAMRAFTDARTADTGD
CCCCCCEEEECCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
EIWLVEHPPVYTLGQAGDPSHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLLDLRRRK
EEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHH
LMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCC
GLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVRRLIANLDGASAAA
CEEEEEEECCCCEEEECCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DKPQALEQHSND
CCCHHHHHCCCC
>Mature Secondary Structure 
SVSPVSIVSTPVAVSASPAGSPDQPAPPVTVRWRGVETYEASFDAMRAFTDARTADTGD
CCCCCEEEECCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCC
EIWLVEHPPVYTLGQAGDPSHLLVADSGVPLVKVDRGGQITYHGPGQIVAYLLLDLRRRK
EEEEEECCCEEEECCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHH
LMVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCC
GLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVAHTLVRRLIANLDGASAAA
CEEEEEEECCCCEEEECCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
DKPQALEQHSND
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA