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The map label for this gene is amtB [H]

Identifier: 78067663

GI number: 78067663

Start: 3341542

End: 3343056

Strand: Reverse

Name: amtB [H]

Synonym: Bcep18194_A6194

Alternate gene names: 78067663

Gene position: 3343056-3341542 (Counterclockwise)

Preceding gene: 78067664

Following gene: 78067662

Centisome position: 90.5

GC content: 68.05

Gene sequence:

>1515_bases
ATGCGCAAACTTCTGATGTCCCTGCTGATGGCCGGCTCGCTGATCGCGGCCGGCGTCGGCTCCGCGCTCGCCGACGACGC
GGCGTCCGCCGCGGCCGCGTCCGCCCCCGCCGCAATGGCTTCCGACGCTTCGGCTGCCGCCGCTCCGGCCGCTTCGGCAC
CGGCTGCCACCGACGCATCCGCCACGGCCGCTGCCGCCGCACCGGCCTCGGGCGCCACCGATGCATCGGCCGCCGCTGCC
GCTTCGGCCCCGGCCGCACCGGCCGCGCCGACCGAGCCGTTCACGGTCGATTCGTCGAAGATCAGTGCGGGCGACACCGC
GTGGATGCTGACCTCCACCGCGCTCGTGCTGTTCATGACGATCCCCGGCCTCGCGCTGTTCTACGCCGGCATGGTCCGCA
AGAAGAACGTGCTCGCGACGGTGATGCAGAGCTTCGCGATCACCGCGGTGATCACGGTGCTGTGGACGGTGGTCGGCTAC
AGCCTCGCGTTCACGCCGGGCAACGGCTTCATTGGCGGCCTGTCGCGTGCGTTCCTGCACGGGATGACCTACGTCAAGGG
CGACAAGGCGACGACGCTGACCGTCAGCCACCTGGCGACGACGATTCCGGAATCGGTCTACTTCGTCTACCAGATGACGT
TCGCGATCATCACACCGGCCCTGATCTGCGGCGCGTTCGCCGACCGGATGAAGTTTTCGGCGATGCTCGTGTTCATGACG
CTCTGGTCGCTGATCGTCTATGTGCCGATCGCGCACATGGTGTGGGAGCCGACCGGCTGGCTGTCGTCGGATGGCGTGCT
CGACTTCGCGGGCGGCACGGTGGTGCACATCAACGCCGGTATCGCGGGCCTCGTGTCATGCCTGATGCTCGGCAAGCGCA
TCGGCTTCGGTCGTGAATCGATGGCGCCGCACAACCTCGTGCTGACGATGATCGGCGGCTCGATGCTGTGGGTCGGCTGG
TTCGGCTTCAACGCGGGTTCCGCAGTCGCGGCCGACGGCCGTGCCGGTTTCGCGATGCTGACGACGCAAGTCGCGACCGC
CTGCGCGGCGCTCGGCTGGATGTTCGCCGAGTGGATCTCGCACGGCAAGCCGTCGGTGCTCGGCATCGTGTCGGGTGCGG
TCGCGGGTCTCGTCGCGATCACGCCGGCAGCCGGCTTCGTCGGCGTGACGGGTGCACTCGTGATCGGCATCGCAGCCGGC
GTCGTGTGCTTCTGGTCGGCGACGTGGCTCAAGTCGAAGCTCGGCTACGACGATTCGCTCGACGCATTCGGCGTGCACGG
CGTGGGCGGGATTCTCGGCGCGCTGCTGACAGGCGTGTTCGCGGTCAAGGACATCGGCGGCGCCGACGGCAGCCTGCTGC
TGCAGGCCAAGGGCGTGCTGATCACGCTGGTCTACAGCGGCGTGCTGAGCTTCGTGCTGCTGAAGCTGATCGATCTCACG
ATCGGCCTGCGCGTGTCGGAAGAAGAAGAGCGCGAAGGCCTCGACGTGGTCCTGCACGGCGAGCATGTCGAATAA

Upstream 100 bases:

>100_bases
GTCACGGCGATCGAACAGGTAATCCGGATTCGCACCGGGGAGACCGGCGCAGACGCGCTGTAACAGAACAGACAAGACAA
GCGACAAGAGGAAACCCAAG

Downstream 100 bases:

>100_bases
GTCACAAGAATGACCGGCGGGCCGCCGCGACGACGGCGCCCGCCATGAATGAGCGCAGCGACTTCGGCCCGCCCCACCCG
GCGGGCTTTTTTTCTCCTGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRKLLMSLLMAGSLIAAGVGSALADDAASAAAASAPAAMASDASAAAAPAASAPAATDASATAAAAAPASGATDASAAAA
ASAPAAPAAPTEPFTVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGY
SLAFTPGNGFIGGLSRAFLHGMTYVKGDKATTLTVSHLATTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMT
LWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVHINAGIAGLVSCLMLGKRIGFGRESMAPHNLVLTMIGGSMLWVGW
FGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWISHGKPSVLGIVSGAVAGLVAITPAAGFVGVTGALVIGIAAG
VVCFWSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLLLQAKGVLITLVYSGVLSFVLLKLIDLT
IGLRVSEEEEREGLDVVLHGEHVE

Sequences:

>Translated_504_residues
MRKLLMSLLMAGSLIAAGVGSALADDAASAAAASAPAAMASDASAAAAPAASAPAATDASATAAAAAPASGATDASAAAA
ASAPAAPAAPTEPFTVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGY
SLAFTPGNGFIGGLSRAFLHGMTYVKGDKATTLTVSHLATTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMT
LWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVHINAGIAGLVSCLMLGKRIGFGRESMAPHNLVLTMIGGSMLWVGW
FGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWISHGKPSVLGIVSGAVAGLVAITPAAGFVGVTGALVIGIAAG
VVCFWSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLLLQAKGVLITLVYSGVLSFVLLKLIDLT
IGLRVSEEEEREGLDVVLHGEHVE
>Mature_504_residues
MRKLLMSLLMAGSLIAAGVGSALADDAASAAAASAPAAMASDASAAAAPAASAPAATDASATAAAAAPASGATDASAAAA
ASAPAAPAAPTEPFTVDSSKISAGDTAWMLTSTALVLFMTIPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGY
SLAFTPGNGFIGGLSRAFLHGMTYVKGDKATTLTVSHLATTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMT
LWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVHINAGIAGLVSCLMLGKRIGFGRESMAPHNLVLTMIGGSMLWVGW
FGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWISHGKPSVLGIVSGAVAGLVAITPAAGFVGVTGALVIGIAAG
VVCFWSATWLKSKLGYDDSLDAFGVHGVGGILGALLTGVFAVKDIGGADGSLLLQAKGVLITLVYSGVLSFVLLKLIDLT
IGLRVSEEEEREGLDVVLHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=406, Percent_Identity=51.2315270935961, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=29.490022172949, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=464, Percent_Identity=26.7241379310345, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32564042, Length=438, Percent_Identity=27.6255707762557, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=449, Percent_Identity=24.7216035634744, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=36.7441860465116, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6325396, Length=428, Percent_Identity=35.2803738317757, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=33.4928229665072, Blast_Score=210, Evalue=5e-55,
Organism=Drosophila melanogaster, GI161078314, Length=421, Percent_Identity=27.3159144893112, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51079; Mature: 51079

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLMSLLMAGSLIAAGVGSALADDAASAAAASAPAAMASDASAAAAPAASAPAATDAS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCHHCCCCCCCCCCCCCC
ATAAAAAPASGATDASAAAAASAPAAPAAPTEPFTVDSSKISAGDTAWMLTSTALVLFMT
CHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
IPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAFTPGNGFIGGLSRAFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
GMTYVKGDKATTLTVSHLATTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMT
CCCEEECCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVHINAGIAGLVSCLMLGKRIGFGRES
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
MAPHNLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIS
CCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HGKPSVLGIVSGAVAGLVAITPAAGFVGVTGALVIGIAAGVVCFWSATWLKSKLGYDDSL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAFGVHGVGGILGALLTGVFAVKDIGGADGSLLLQAKGVLITLVYSGVLSFVLLKLIDLT
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IGLRVSEEEEREGLDVVLHGEHVE
HCCEECCHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MRKLLMSLLMAGSLIAAGVGSALADDAASAAAASAPAAMASDASAAAAPAASAPAATDAS
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHCCCCHHCCCCCCCCCCCCCC
ATAAAAAPASGATDASAAAAASAPAAPAAPTEPFTVDSSKISAGDTAWMLTSTALVLFMT
CHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHH
IPGLALFYAGMVRKKNVLATVMQSFAITAVITVLWTVVGYSLAFTPGNGFIGGLSRAFLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHH
GMTYVKGDKATTLTVSHLATTIPESVYFVYQMTFAIITPALICGAFADRMKFSAMLVFMT
CCCEEECCCCEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWSLIVYVPIAHMVWEPTGWLSSDGVLDFAGGTVVHINAGIAGLVSCLMLGKRIGFGRES
HHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
MAPHNLVLTMIGGSMLWVGWFGFNAGSAVAADGRAGFAMLTTQVATACAALGWMFAEWIS
CCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HGKPSVLGIVSGAVAGLVAITPAAGFVGVTGALVIGIAAGVVCFWSATWLKSKLGYDDSL
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DAFGVHGVGGILGALLTGVFAVKDIGGADGSLLLQAKGVLITLVYSGVLSFVLLKLIDLT
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
IGLRVSEEEEREGLDVVLHGEHVE
HCCEECCHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]