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The map label for this gene is rfaF [H]

Identifier: 78067433

GI number: 78067433

Start: 3093607

End: 3094596

Strand: Direct

Name: rfaF [H]

Synonym: Bcep18194_A5964

Alternate gene names: 78067433

Gene position: 3093607-3094596 (Clockwise)

Preceding gene: 78067432

Following gene: 78067434

Centisome position: 83.74

GC content: 67.88

Gene sequence:

>990_bases
ATGGCGCAGCCACTTTTTGCGCTGCTGAAGAAAAACCATCCCCGCATCGCGATCGATGCCGTCGCACCCTCGTGGGTCGC
GCCCGTGCTCGAACGGATGCCCGAGATCCACGATGTCTACGCGACCGATCTCGCGCACGGCAAGCTGCAGTTGCTGCGCC
GCTGGCAACTCGCGAGCGATCTGCGCGACGTCGGCTACGACGCGGCGTACGTGCTGCCGAATTCGCTGAAATCCGCGGTG
ATCCCGTGGCTCGCGAACATCCCGCTGCGGGTCGGCTACACGGGCGAGCACCGCTACGGGCTGCTGAACGTGCGGCACGC
GAACCCCGACAAGTCGGGCGAGCGCCCGCCGATGACGACGCACTACGCGGCGCTCGCGTACGCGCCGGGCGCGAAGCTGC
CCGAGTCGATGAAGACGCTGCCCGCGCCGCGCCTCGACGCCGATCTCAACGAGACGGCGCGCGTGTCCGCGCGTTTCAAT
CTCGATACACGCAAGCCGCTCGTCGTGTTCTGCCCCGGCGCGGAATACGGCCCGGCCAAGCGCTGGCCGCCCGAACACTT
CGCGTCGCTCGCCACCATCGTCCATCAGTCGTTCCCGTACACGCAGATCGTCGCGCTCGGTTCGCAGAAGGATGCCGCGG
CTGCGCAGGCGATCGCCGACCACGCACCGAACGTGCGCAACCTGTGCGGGCAGACGTCGCTGTCGGAGGCCTGCGCACTG
ATCGCGCGCGCGAACGCGGTGGTCACCAACGATTCGGGGCTGATGCACGTTGCCGCCGCGCTACGCCGGCCGCTCGTCGC
GCTGTACGGATCGACCGATCCGCGCCACACCCCTCCGCTGTCGGACCTGGCGAAGGTACAATGGCTTCATCTCGAATGCA
GTCCCTGCTTCGAACGCGAGTGCCCGCTCGGCCACCTGAAGTGCCTGCGCGAACTCGGTCCCGAGCAGGTATTCGGCGAT
TTGCGCGGCATGCTCGTCGGGCAACGCTGA

Upstream 100 bases:

>100_bases
CGAACGCGGCGCCTGGGCGCCGTGCTTCATTACGACATCGGAAGTCGACCTGATGCGTCGAGCGCTGGTTATCGCACCGA
ACTGGATCGGTGACGCATTG

Downstream 100 bases:

>100_bases
CCCTGGCCGGCAGCGTCGCACGGCGCGCCGGGCAATCCGATTCACCGGTGTACGGCGGCTCCATGCGGGCCGCCGTGCTG
AATACGGCGCGCGACGCGCG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAQPLFALLKKNHPRIAIDAVAPSWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAV
IPWLANIPLRVGYTGEHRYGLLNVRHANPDKSGERPPMTTHYAALAYAPGAKLPESMKTLPAPRLDADLNETARVSARFN
LDTRKPLVVFCPGAEYGPAKRWPPEHFASLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGD
LRGMLVGQR

Sequences:

>Translated_329_residues
MAQPLFALLKKNHPRIAIDAVAPSWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAV
IPWLANIPLRVGYTGEHRYGLLNVRHANPDKSGERPPMTTHYAALAYAPGAKLPESMKTLPAPRLDADLNETARVSARFN
LDTRKPLVVFCPGAEYGPAKRWPPEHFASLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGD
LRGMLVGQR
>Mature_328_residues
AQPLFALLKKNHPRIAIDAVAPSWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASDLRDVGYDAAYVLPNSLKSAVI
PWLANIPLRVGYTGEHRYGLLNVRHANPDKSGERPPMTTHYAALAYAPGAKLPESMKTLPAPRLDADLNETARVSARFNL
DTRKPLVVFCPGAEYGPAKRWPPEHFASLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACALI
ARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERECPLGHLKCLRELGPEQVFGDL
RGMLVGQR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=339, Percent_Identity=41.5929203539823, Blast_Score=242, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 36049; Mature: 35918

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPLFALLKKNHPRIAIDAVAPSWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASD
CCCHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDVGYDAAYVLPNSLKSAVIPWLANIPLRVGYTGEHRYGLLNVRHANPDKSGERPPMTT
HHHCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCH
HYAALAYAPGAKLPESMKTLPAPRLDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAK
HHHHEEECCCCCCCHHHHHCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCCCC
RWPPEHFASLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERE
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCCC
CPLGHLKCLRELGPEQVFGDLRGMLVGQR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQPLFALLKKNHPRIAIDAVAPSWVAPVLERMPEIHDVYATDLAHGKLQLLRRWQLASD
CCHHHHHHHCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDVGYDAAYVLPNSLKSAVIPWLANIPLRVGYTGEHRYGLLNVRHANPDKSGERPPMTT
HHHCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCH
HYAALAYAPGAKLPESMKTLPAPRLDADLNETARVSARFNLDTRKPLVVFCPGAEYGPAK
HHHHEEECCCCCCCHHHHHCCCCCCCCCCCCHHEEEEEECCCCCCCEEEEECCCCCCCCC
RWPPEHFASLATIVHQSFPYTQIVALGSQKDAAAAQAIADHAPNVRNLCGQTSLSEACAL
CCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHH
IARANAVVTNDSGLMHVAAALRRPLVALYGSTDPRHTPPLSDLAKVQWLHLECSPCFERE
HHHCCEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHEEEEEEECCCCCCCC
CPLGHLKCLRELGPEQVFGDLRGMLVGQR
CCHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]