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The map label for this gene is oprM [H]

Identifier: 78067407

GI number: 78067407

Start: 3063627

End: 3065150

Strand: Reverse

Name: oprM [H]

Synonym: Bcep18194_A5938

Alternate gene names: 78067407

Gene position: 3065150-3063627 (Counterclockwise)

Preceding gene: 78067408

Following gene: 78067405

Centisome position: 82.97

GC content: 67.78

Gene sequence:

>1524_bases
ATGATGCAAAAACACGCTTTGACTGCAATCGCGGTCGCGCTCTTCGCCACGGGCTGCACGATGGCGCCGCATTACAAGCG
GCCCGACGCACCCGTCGCGCAGGCGTACCCGGCCGGCGGCGTCTACGCGACGCAGCCGGGGGCGGCCGGCGCGCGTAGCG
CGAACGGCCAGGCGGCGACCGCCATCGGCTGGCGCGAGTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATCGCG
CTGAAAAACAACCGCGACCTGCGCGTGTCGGTGCTGAACATCGAGGCGGCGCGCGCGCAGTACCAGATCACGCGCGCGGG
GCTGTTCCCGACGCTCGATGGCACCGGTACGGGCAACGTCCAGCGTATCCCGCAGGGCCTGTCGACGACGGGTGCGCCGA
TGATCTCGCGCAGCTACAACGTCGGGCTGTCGGCGTCGTGGGAGCTCGACCTGTTCGGGCGCGTGCAGAGCCTGAAGGAC
CAGGCGCTCGCGCAATACCTGTCGACGTCTTATGCGCGGCAAGCGCAGGAGATCTCGCTGGTATCGCAGGTGGCTGATCA
ATACCTGACGCTGCTGTCGACCGACGACCTGCTGAAGGTCACGGAGGACACGCTGAAGTCCGCGCAGGACTCGTACAACC
TGACGAAGCTGAAGTTCGACAACGGCACGGGCTCCGAGCTCGACCTGCGTCAGGCGCAGACGGTGGTCGAGACGGCGCTC
GCAAGCCAGCAGTCGCAGGCGCGCGACCGTGCGCAGGCGCTGAACTATCTGGTACTGCTGATCGGCGAGCCGCTGCCGGA
CGACCTGCCCGCGGGCATGCCGCTCGACGCGCAGAACCTGCTGACGGACGTGCCGGCAGGCCTGCCGTCCGACCTGCTGA
CGCGTCGTCCCGACGTGATGCAGGCGGAGCAGACGCTGCTGGCCGCGAATGCGAACATCGGCGCGGCCCGCGCGGCGTTC
TTCCCGAAGATCTCGCTGACAGGCGCGTTCGGCACCGGGAGCCCGACGCTGGGCGGTCTGTTCAAGGCCGGCACGGCGGC
GTGGTCGTTCGCGCCGCAGATCACGATGCCGATCTTCGAAGGTGGCCAGAACATCGCGAACCTGAACCTTGCGCACGTGC
AGAAGCGCATCGAGATCGCCAACTACGAGAAGGCGATCCAGTCGGCGTTCCGCGAAGTGGCTGACGGATTGGCGGCACGC
GGCACGTACGACCAGCAGATCGCGGCGCTCGAGCGCAACGAGCACGCGCAGCAGCGACGTTACGACCTGTCGGACCTGCG
TTACAAGAACGGTGTCGACAGCTACCTGTCGGTGCTGACCGCGCAGACGGATCTGTACAACGCACAGCAGTCGCTGATCA
GCGCACGTCTGGCACGCTGGACGAACCTGGTCACCCTGTACCGTGCGCTGGGCGGCGGGTGGGTTCAGCGCGCCGGCGAA
ACGCCGCGTGCGCCGGATGCACCGGTCGACTACGACAAGGCGGCCGCTCCGGCGCCTGCGTCGGCAGCAGCGACGAACGG
GTAA

Upstream 100 bases:

>100_bases
GCGAGAAGGAAGATGCCGACGAAGCACTGCGTCTGGCTCAGGAGCACGCGCGCCATGAAGAGAAGCCGGGCAACGGCGAC
GAAGGCAACAAGGGACAGTG

Downstream 100 bases:

>100_bases
GCAAGCGCAAGGAAGGGCAGGAGCGGTCTACCGCGCTTGCCCTTCGAGCCGGCTGTATGCGGAAACGAAAACGCCACGGA
CGAAAGTCCGTGGCGTTTTC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNVQRIPQGLSTTGAPMISRSYNVGLSASWELDLFGRVQSLKD
QALAQYLSTSYARQAQEISLVSQVADQYLTLLSTDDLLKVTEDTLKSAQDSYNLTKLKFDNGTGSELDLRQAQTVVETAL
ASQQSQARDRAQALNYLVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTGSPTLGGLFKAGTAAWSFAPQITMPIFEGGQNIANLNLAHVQKRIEIANYEKAIQSAFREVADGLAAR
GTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYNAQQSLISARLARWTNLVTLYRALGGGWVQRAGE
TPRAPDAPVDYDKAAAPAPASAAATNG

Sequences:

>Translated_507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNVQRIPQGLSTTGAPMISRSYNVGLSASWELDLFGRVQSLKD
QALAQYLSTSYARQAQEISLVSQVADQYLTLLSTDDLLKVTEDTLKSAQDSYNLTKLKFDNGTGSELDLRQAQTVVETAL
ASQQSQARDRAQALNYLVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTGSPTLGGLFKAGTAAWSFAPQITMPIFEGGQNIANLNLAHVQKRIEIANYEKAIQSAFREVADGLAAR
GTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYNAQQSLISARLARWTNLVTLYRALGGGWVQRAGE
TPRAPDAPVDYDKAAAPAPASAAATNG
>Mature_507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNVQRIPQGLSTTGAPMISRSYNVGLSASWELDLFGRVQSLKD
QALAQYLSTSYARQAQEISLVSQVADQYLTLLSTDDLLKVTEDTLKSAQDSYNLTKLKFDNGTGSELDLRQAQTVVETAL
ASQQSQARDRAQALNYLVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTGSPTLGGLFKAGTAAWSFAPQITMPIFEGGQNIANLNLAHVQKRIEIANYEKAIQSAFREVADGLAAR
GTYDQQIAALERNEHAQQRRYDLSDLRYKNGVDSYLSVLTAQTDLYNAQQSLISARLARWTNLVTLYRALGGGWVQRAGE
TPRAPDAPVDYDKAAAPAPASAAATNG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=473, Percent_Identity=40.5919661733615, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI2367348, Length=425, Percent_Identity=25.4117647058824, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54412; Mature: 54412

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAAT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCEEE
AIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNV
EHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCH
QRIPQGLSTTGAPMISRSYNVGLSASWELDLFGRVQSLKDQALAQYLSTSYARQAQEISL
HHCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQVADQYLTLLSTDDLLKVTEDTLKSAQDSYNLTKLKFDNGTGSELDLRQAQTVVETAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ASQQSQARDRAQALNYLVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVM
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHH
QAEQTLLAANANIGAARAAFFPKISLTGAFGTGSPTLGGLFKAGTAAWSFAPQITMPIFE
HHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHCCCCCEECHHHC
GGQNIANLNLAHVQKRIEIANYEKAIQSAFREVADGLAARGTYDQQIAALERNEHAQQRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YDLSDLRYKNGVDSYLSVLTAQTDLYNAQQSLISARLARWTNLVTLYRALGGGWVQRAGE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
TPRAPDAPVDYDKAAAPAPASAAATNG
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAAT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCEEE
AIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNV
EHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCH
QRIPQGLSTTGAPMISRSYNVGLSASWELDLFGRVQSLKDQALAQYLSTSYARQAQEISL
HHCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQVADQYLTLLSTDDLLKVTEDTLKSAQDSYNLTKLKFDNGTGSELDLRQAQTVVETAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ASQQSQARDRAQALNYLVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVM
HHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHH
QAEQTLLAANANIGAARAAFFPKISLTGAFGTGSPTLGGLFKAGTAAWSFAPQITMPIFE
HHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHCCCCCEECHHHC
GGQNIANLNLAHVQKRIEIANYEKAIQSAFREVADGLAARGTYDQQIAALERNEHAQQRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YDLSDLRYKNGVDSYLSVLTAQTDLYNAQQSLISARLARWTNLVTLYRALGGGWVQRAGE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
TPRAPDAPVDYDKAAAPAPASAAATNG
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]