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The map label for this gene is ogt [H]

Identifier: 78067361

GI number: 78067361

Start: 3012514

End: 3013017

Strand: Reverse

Name: ogt [H]

Synonym: Bcep18194_A5892

Alternate gene names: 78067361

Gene position: 3013017-3012514 (Counterclockwise)

Preceding gene: 78067362

Following gene: 78067360

Centisome position: 81.56

GC content: 67.06

Gene sequence:

>504_bases
GTGGTGCCGCGCGCATCCATCGGTCAGGAGAGGGCCATGTTCAATGCAGTCATCGACGCGCCGTTCGGCAAAGTCGGCAT
CCGCACGGACGCCGCGGTGGTGCGCGAGATCGTCTATCTGCCCGAATCGGTGAAGTCGGTCGATCCGGATTCGCCGCTCG
CGAAGCGCGCGGTCAAGCAGATCGAACGTTATTTCGAGCGCGCATCGGCGCGCTTCGACCTGCCGCTCGCCGAAGTCGGC
AGCGCGTTCCAGCACCGCGTGTGGGACGTGATCAGCGATATCCCGCCCGGCACGGTGCTGACCTACGGGCAGGTCGCGAA
GCGGATCGGCAGCGCGCCGCGCGCGGTCGGCCAGGCGTGCGGCGCGAACTACTTCCCGCTCGTGATTCCATGCCATCGCG
TCGTCGCGGCGGGCGGCCTCGGCGGCTTCGCGAACCACGACGACAACGGTTATTACCAGAAAGTGAAGCGCTGGCTGCTG
GCGCACGAAGGCGTGCCGTACTGA

Upstream 100 bases:

>100_bases
TTGCGCGCGCGGGCCGACGATCCGTCGCCGGTCGTGCGCGAGCATGTCGAATGGGCGCTGCGTGCCGTGTGAAGGCGGCG
TAAAGTGTCGTCGGCGCGCG

Downstream 100 bases:

>100_bases
TGAGTGAATCGCTGATTTCTCCCGAAGCCGACGGCGATGCCGCCGCGGCGTCACCCGCGCTGCTCGCGAGCCGTGCGTCG
ATCGACGTGTTTTGCGATGC

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MVPRASIGQERAMFNAVIDAPFGKVGIRTDAAVVREIVYLPESVKSVDPDSPLAKRAVKQIERYFERASARFDLPLAEVG
SAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQKVKRWLL
AHEGVPY

Sequences:

>Translated_167_residues
MVPRASIGQERAMFNAVIDAPFGKVGIRTDAAVVREIVYLPESVKSVDPDSPLAKRAVKQIERYFERASARFDLPLAEVG
SAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQKVKRWLL
AHEGVPY
>Mature_167_residues
MVPRASIGQERAMFNAVIDAPFGKVGIRTDAAVVREIVYLPESVKSVDPDSPLAKRAVKQIERYFERASARFDLPLAEVG
SAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQKVKRWLL
AHEGVPY

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=133, Percent_Identity=37.593984962406, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788542, Length=117, Percent_Identity=34.1880341880342, Blast_Score=70, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=114, Percent_Identity=36.8421052631579, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18206; Mature: 18206

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRASIGQERAMFNAVIDAPFGKVGIRTDAAVVREIVYLPESVKSVDPDSPLAKRAVKQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
IERYFERASARFDLPLAEVGSAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQAC
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHH
GANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQKVKRWLLAHEGVPY
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVPRASIGQERAMFNAVIDAPFGKVGIRTDAAVVREIVYLPESVKSVDPDSPLAKRAVKQ
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHH
IERYFERASARFDLPLAEVGSAFQHRVWDVISDIPPGTVLTYGQVAKRIGSAPRAVGQAC
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHH
GANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQKVKRWLLAHEGVPY
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]