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The map label for this gene is braE [H]

Identifier: 78067297

GI number: 78067297

Start: 2942133

End: 2943302

Strand: Reverse

Name: braE [H]

Synonym: Bcep18194_A5828

Alternate gene names: 78067297

Gene position: 2943302-2942133 (Counterclockwise)

Preceding gene: 78067298

Following gene: 78067296

Centisome position: 79.68

GC content: 62.99

Gene sequence:

>1170_bases
ATGACATCCATTCAACCGATCGAATCCTCGACGTCGCTCGTCGAAGAGCGCAACACCACCAAGACCATCATCATCGGTGT
CCTGACCGCGGTCTTCGTGATCGCGGCGCCGATCATCATCGGCTCGGCCGGCGGCAACTACTGGGTCCGTGTGCTCGACT
TCGCGATGCTGTACGTGATGCTCGCGCTGGGTCTTAACGTGGTGGTCGGCTTCGCCGGCCTGCTCGACCTGGGCTACATC
GCGTTCTACGCGGTGGGCGCGTACACGGCGGCGTTGTTGAGCTCGCCGCACCTGAGCTCGCAATTCGAGTGGATCGCGGC
GCTGGCGCCGAACGGATTGCATTTTCCGTTCTTCGTCATCGTGCCGATCGCGATGGCACTGGCGGCGACCTTCGGGATCC
TGCTCGGCGCGCCGACGTTGCGCCTGCGCGGCGACTACCTGGCGATCGTGACGCTCGGCTTCGGTGAAATCGTCCGGATC
TTCATGAACAACCTCGACCGTCCGGTGAACATCACCAACGGCCCGAAGGGGATCACGGGCATCGATCCGGTGCACATCGG
CGACTTCAGCCTCGCGCAGACCCATTCGCTGTTCGGGATCCAGCTGCCGTCGGTCTACATGTATTACTACCTGTTCGTGC
TGTGCTCGCTGCTGGTGATTTGGGTGTGTACGCGCCTGCAGCACTCGCGTATCGGCCGCGCATGGGCGGCGATCCGCGAA
GACGAAATCGCGGCGAAGGCGATGGGCATCAACACCCGTAACGTGAAGCTGCTCGCATTCGCGATGGGCGCGTCGTTCGG
CGGCCTGTCGGGTGCGATGTTCGGTTCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGTTCTGGGAGTCGATCGTCG
TGCTCGCCTGCGTGGTGCTCGGCGGCATGGGCCACATCCCGGGCGTGATCCTGGGTGCGGTGCTGCTCGCGATCTTCCCG
GAATTCCTGCGCTCGACGATGAGCCCGCTGCAGCACGCGATCTTCGGCCATGACGTCGTCGATACCGAAGTGATCCGTCA
GGCGCTGTACGGCCTCGCGATGGTGGTCATCATGCTGTACCGCTCGGAAGGCCTGTGGCCCGCACCGAAGCATGAGGACA
AGATCGCGAAACTGGCGAAGCGCAACAGCAAGAAGCCGGTTCGTGCTTAA

Upstream 100 bases:

>100_bases
GTTCGCCTTCATCGTGCTGATCATCGTGCTGGTGTTCCGTCCGTCCGGCCTGCTGGGCGAACGTGTCGCGGATCGCGCGT
AACGGAAGGGAGCAAACAAC

Downstream 100 bases:

>100_bases
TCGACGGACACAGGGGATATACACATGAGCGACAAACAAATCCGACTGTCCGTCAAAGGCGTGAACAAGCGCTTCGGTGG
CCTGCAGGCGCTGTCCGACG

Product: branched chain amino acid ABC transporter inner membrane protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIESSTSLVEERNTTKTIIIGVLTAVFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLSSQFEWIAALAPNGLHFPFFVIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHIGDFSLAQTHSLFGIQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFP
EFLRSTMSPLQHAIFGHDVVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIESSTSLVEERNTTKTIIIGVLTAVFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLSSQFEWIAALAPNGLHFPFFVIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHIGDFSLAQTHSLFGIQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFP
EFLRSTMSPLQHAIFGHDVVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA
>Mature_388_residues
TSIQPIESSTSLVEERNTTKTIIIGVLTAVFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLSSQFEWIAALAPNGLHFPFFVIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNITNGPKGITGIDPVHIGDFSLAQTHSLFGIQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFPE
FLRSTMSPLQHAIFGHDVVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=348, Percent_Identity=41.9540229885057, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42098; Mature: 41967

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQPIESSTSLVEERNTTKTIIIGVLTAVFVIAAPIIIGSAGGNYWVRVLDFAMLYVM
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLSSQFEWIAALAPNGLHFPFFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHH
VPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHIGDFSLAQTHSLFGIQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAIFPEFLRSTMSPLQHAIFGHDVVDTEVIRQALYGLAMVVIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNSKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSIQPIESSTSLVEERNTTKTIIIGVLTAVFVIAAPIIIGSAGGNYWVRVLDFAMLYVM
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLSSQFEWIAALAPNGLHFPFFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHH
VPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHIGDFSLAQTHSLFGIQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAIFPEFLRSTMSPLQHAIFGHDVVDTEVIRQALYGLAMVVIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNSKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]