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The map label for this gene is nadE

Identifier: 78067208

GI number: 78067208

Start: 2843696

End: 2845402

Strand: Reverse

Name: nadE

Synonym: Bcep18194_A5739

Alternate gene names: 78067208

Gene position: 2845402-2843696 (Counterclockwise)

Preceding gene: 78067210

Following gene: 78067207

Centisome position: 77.03

GC content: 67.49

Gene sequence:

>1707_bases
ATGAAGACCCGACTCGCTCTCGCCCAGATCAACGTCACCGTCGGCGACTTCGCCGGCAACGTCGCGCGGATCGTCGCGGC
CGCACGCGCCGCGCACAACGATGGTGCGCAGCTGATGGTCGCGCCGGAACTCGCGCTGTCCGGCTATCCGCCGGAAGACC
TGCTGCTGCGGCCCGCGTTCTATGCAGCGGCGGCCTCGGCACTCGACGCGCTCGCCGATGCGCTGAAGGCATTCGACGGG
CTCGCGGTGCTGGTCGGCCATCCGCTACGCGGCGCAGGCGAAGGCGCGCACGCGCCAGCCGTCGATGGTAATGCAAACCG
CCCGATCGAGCGCGGCGTGCCGCCCACCGATACCTACAACGCGGTATCGCTGATCGTCGGCGGCGAGATCGTCGGCACTT
ATCGCAAGCAGGATCTGCCGAACGCAGAAGTGTTCGACGAGAAGCGCTACTTCGCGACGGACGCCGAGCCGCTCGTGTTC
GAGCTGAACGGCGTGAAGTACGGCGTGATCATCTGCGAGGATGCGTGGCACGCATCGGCCGCGCAGATCGCGAAGGCGGC
CGGCGCGCAGGTGCTGCTGATCCCGAACGGCTCGCCGTACCACATGAACAAGGAAGCGGTGCGCTTCGACATCCTGCGCG
CGCGGATTCGCGAAACCGGGCTGCCGATGGTGTACATCAACCTCGTCGGCGGGCAGGACGAGCTCGTGTTCGACGGCGGC
TCGTTCGTGCTCGATGCGCAGGGCGCGCTCGTCGTGAAGATGCCGCAGTTCGACGAAGGGCACGCGATCGTCGAATTCGA
CGGCGCGCGGCCGCTGCCGGGCGCGATTGCGCCCGAGCTGTCGACGGACGCGCAGGTGTACCGTGCGCTCGTGACCGGCG
TGCGCGACTACATTGGCAAGAACGGGTTTCCGGGTGCGCTGATCGGCTTGTCGGGCGGTGTCGACTCGGCGCTGGTGCTG
GCCGTCGCCTGCGATGCGCTCGGGCCCGAGCGTGTGCGCGCGGTGATGATGCCGTCGCGCTTCACGGCCGACATCTCGAC
GACCGACGCGGCGGAGATGGCGCGGCGCGTCGGCGTGCGCTACGACGAGATCGCGATCGCGCCGATGTTCGATGCATTCC
GCGCATCGCTCGCGAGTGAGTTCGCGGGCCGCGCGGAAGACGCGACGGAGGAAAACATCCAGGCGCGCATCCGCGGCACG
CTGCTGATGGCGCTGTCGAACAAGTTCGGCTCGATCGTGCTGACGACCGGCAACAAGAGCGAGATGGCGGTCGGCTACTG
CACGCTATACGGCGACATGGCTGGCGGGTTCGCGGTGATCAAGGACATCGCGAAGACGCTCGTGTACCGGCTCTGCCGCT
ATCGCAACGAAACCACCGCGTACCCGCTGCGCGACGTGATCCCCGAACGGATCCTCACGCGCGCGCCGTCGGCCGAGCTG
CGCGAGAACCAGACCGACCAGGACAGCCTGCCGCCGTACGACGTGCTCGACGCGATCATGCGGATGTACATGGAAGAGGA
CCGGCCGCTCGCCGAGATCGTCGCGGCCGGTTATGCGCAGACCGACGTCGAACGCGTGACGCGGCTCATCAAGATCAACG
AATACAAGCGCCGCCAGGCGCCGATCGGCATTCGCGTCACGCATCGCGCGTTCGGGCGCGACTGGCGCTACCCGATCACG
TCACGTTTTACCGAACGCCTCGATTGA

Upstream 100 bases:

>100_bases
GTACCGTTCTGTGTCGCCGGATGCGAGGGCCTGCGCGGCCCGCACCGGGACGATTTCGTCAGCCGTTCAGGGCGCGCCGC
CGGGCTGCGCCGATCCCGCC

Downstream 100 bases:

>100_bases
GCGGTGCTGGGCCGCATGCGGCTCGCAGGCTTACAATCGGGGTCGCGATTTTTCATCCAAACGAGCATTGGGGGACAACC
ACCATGAAACGCATCACTGC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAASALDALADALKAFDG
LAVLVGHPLRGAGEGAHAPAVDGNANRPIERGVPPTDTYNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDAEPLVF
ELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRFDILRARIRETGLPMVYINLVGGQDELVFDGG
SFVLDAQGALVVKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGALIGLSGGVDSALVL
AVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRASLASEFAGRAEDATEENIQARIRGT
LLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNETTAYPLRDVIPERILTRAPSAEL
RENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQTDVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPIT
SRFTERLD

Sequences:

>Translated_568_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAASALDALADALKAFDG
LAVLVGHPLRGAGEGAHAPAVDGNANRPIERGVPPTDTYNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDAEPLVF
ELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRFDILRARIRETGLPMVYINLVGGQDELVFDGG
SFVLDAQGALVVKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGALIGLSGGVDSALVL
AVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRASLASEFAGRAEDATEENIQARIRGT
LLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNETTAYPLRDVIPERILTRAPSAEL
RENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQTDVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPIT
SRFTERLD
>Mature_568_residues
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAFYAAAASALDALADALKAFDG
LAVLVGHPLRGAGEGAHAPAVDGNANRPIERGVPPTDTYNAVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDAEPLVF
ELNGVKYGVIICEDAWHASAAQIAKAAGAQVLLIPNGSPYHMNKEAVRFDILRARIRETGLPMVYINLVGGQDELVFDGG
SFVLDAQGALVVKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGKNGFPGALIGLSGGVDSALVL
AVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVRYDEIAIAPMFDAFRASLASEFAGRAEDATEENIQARIRGT
LLMALSNKFGSIVLTTGNKSEMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNETTAYPLRDVIPERILTRAPSAEL
RENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQTDVERVTRLIKINEYKRRQAPIGIRVTHRAFGRDWRYPIT
SRFTERLD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61467; Mature: 61467

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHEECHHH
YAAAASALDALADALKAFDGLAVLVGHPLRGAGEGAHAPAVDGNANRPIERGVPPTDTYN
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
AVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVKYGVIICEDAWHASA
EEEEEECCCEEECHHHCCCCCCHHHCCCCEEECCCCCEEEEECCEEEEEEEECCCCCCHH
AQIAKAAGAQVLLIPNGSPYHMNKEAVRFDILRARIRETGLPMVYINLVGGQDELVFDGG
HHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
SFVLDAQGALVVKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGK
CEEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NGFPGALIGLSGGVDSALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVR
CCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCC
YDEIAIAPMFDAFRASLASEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKS
CHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEECCCC
EMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNETTAYPLRDVIPERILTRAPSAEL
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHH
RENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQTDVERVTRLIKINEYKRRQA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PIGIRVTHRAFGRDWRYPITSRFTERLD
CCCEEEEHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKTRLALAQINVTVGDFAGNVARIVAAARAAHNDGAQLMVAPELALSGYPPEDLLLRPAF
CCCEEEEEEEEEEEHHHCCHHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCHHHEECHHH
YAAAASALDALADALKAFDGLAVLVGHPLRGAGEGAHAPAVDGNANRPIERGVPPTDTYN
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
AVSLIVGGEIVGTYRKQDLPNAEVFDEKRYFATDAEPLVFELNGVKYGVIICEDAWHASA
EEEEEECCCEEECHHHCCCCCCHHHCCCCEEECCCCCEEEEECCEEEEEEEECCCCCCHH
AQIAKAAGAQVLLIPNGSPYHMNKEAVRFDILRARIRETGLPMVYINLVGGQDELVFDGG
HHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECC
SFVLDAQGALVVKMPQFDEGHAIVEFDGARPLPGAIAPELSTDAQVYRALVTGVRDYIGK
CEEEECCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
NGFPGALIGLSGGVDSALVLAVACDALGPERVRAVMMPSRFTADISTTDAAEMARRVGVR
CCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHEEECCCCEECCCCCCCHHHHHHHHCCC
YDEIAIAPMFDAFRASLASEFAGRAEDATEENIQARIRGTLLMALSNKFGSIVLTTGNKS
CHHEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEECCCC
EMAVGYCTLYGDMAGGFAVIKDIAKTLVYRLCRYRNETTAYPLRDVIPERILTRAPSAEL
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHH
RENQTDQDSLPPYDVLDAIMRMYMEEDRPLAEIVAAGYAQTDVERVTRLIKINEYKRRQA
HCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
PIGIRVTHRAFGRDWRYPITSRFTERLD
CCCEEEEHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]