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The map label for this gene is trmJ [H]

Identifier: 78066859

GI number: 78066859

Start: 2456077

End: 2456946

Strand: Reverse

Name: trmJ [H]

Synonym: Bcep18194_A5390

Alternate gene names: 78066859

Gene position: 2456946-2456077 (Counterclockwise)

Preceding gene: 78066862

Following gene: 78066858

Centisome position: 66.51

GC content: 69.31

Gene sequence:

>870_bases
ATGGCGATACCATACTGCCATTCTGATGAACCGAGTCGTATCGTAGTGGAAACCCCGCAGATCACCGCCGCGCCGTCCGA
GCCGGGCGCGGCCTCGTCCCAGCCGCCGTCCGGCGGCTTCACGTCCACCCGTTTCGTGCTCGTCGAGCCGAGCCATCCCG
GCAATGTCGGGGCGGCCGCCCGCGCGCTGAAGACGATGGGCTTCTCGCGTCTCGTGCTGGTCGCGCCGCGCGTGCCGCAC
GTGCAGAGCGATCCCGAGGCGATCGCGATGGCCAGCGGCGCGGACGATGTCCTCGCGTCCGCGCATGTCGTGCCGACGCT
CGGCGACGCGCTGTCCGGTGTTCACTGGTCGATCGCGCTGACCGCGCGCACGCGCGAATACGGGCCGCCGCGCCTGGCGC
CGCGTGCCGCTGCCACGCAGGCATGCGCACAGGTCGGCACCGGCGACATCGCGCTCGTGTTCGGCAACGAGCGCACAGGC
CTCGCGAACGAGCATGTCGAGCAGTGCAGCGCGCTGGCGCATATCCCCGCGAACCCGGCCTACAGTTCGCTGAATCTCGC
GCAGGCCGTGCAGGTGCTCGCGTACGAGCTGCGTGTCGCGTTTCTCGAGCACGCGAGCGAGCCGTCGGCGCAACCGTCGG
GCGAAGCCGGCACGCTTGCGCAGAGCGACGAGATCGAGCGGATGTACGTGCATCTCGAGAACGCGCTGATCGCGCTCGAT
TTCCTCGATCCGCGCAACCCGAAGAAGCTGATGCCGCGACTGCGGCGCCTGTTCGCGCGCACGGGTCTCGAGCGCGAGGA
GGTCAACATCCTGCGCGGCGTCGCGAAGCACATCCTGCTGAAATCGGGCAAGCCCGACGGCGACGCGTAA

Upstream 100 bases:

>100_bases
CGCGTAAAGGCATGATTTTACCCGATTTCCGGCGGTTTCAGGCGACCGGAGTACGGGGAAACCCCCACTATCCGTCCGGA
CGAGTGGGTTTGCCGCGGCG

Downstream 100 bases:

>100_bases
GCGGTCGGCGCACGGGGTGCCGAATGTCCCTGCACGCGGGGCAATCGCCCCGTCGGCAGGTGCCGGGTCGGCCGCCGGAA
GCGCCGGGGCCGATCGCGCC

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAIPYCHSDEPSRIVVETPQITAAPSEPGAASSQPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAIAMASGADDVLASAHVVPTLGDALSGVHWSIALTARTREYGPPRLAPRAAATQACAQVGTGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEHASEPSAQPSGEAGTLAQSDEIERMYVHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGVAKHILLKSGKPDGDA

Sequences:

>Translated_289_residues
MAIPYCHSDEPSRIVVETPQITAAPSEPGAASSQPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPH
VQSDPEAIAMASGADDVLASAHVVPTLGDALSGVHWSIALTARTREYGPPRLAPRAAATQACAQVGTGDIALVFGNERTG
LANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEHASEPSAQPSGEAGTLAQSDEIERMYVHLENALIALD
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGVAKHILLKSGKPDGDA
>Mature_288_residues
AIPYCHSDEPSRIVVETPQITAAPSEPGAASSQPPSGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVAPRVPHV
QSDPEAIAMASGADDVLASAHVVPTLGDALSGVHWSIALTARTREYGPPRLAPRAAATQACAQVGTGDIALVFGNERTGL
ANEHVEQCSALAHIPANPAYSSLNLAQAVQVLAYELRVAFLEHASEPSAQPSGEAGTLAQSDEIERMYVHLENALIALDF
LDPRNPKKLMPRLRRLFARTGLEREEVNILRGVAKHILLKSGKPDGDA

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=239, Percent_Identity=45.6066945606695, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=38.6075949367089, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30574; Mature: 30443

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPYCHSDEPSRIVVETPQITAAPSEPGAASSQPPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAIAMASGADDVLASAHVVPTLGDALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAATQACAQVGTGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEHASEPSAQPSGEAGTLAQSDEIERMYVHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGVAKHILLKSGKPDGDA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AIPYCHSDEPSRIVVETPQITAAPSEPGAASSQPPSGGFTSTRFVLVEPSHPGNVGAAA
CCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHH
RALKTMGFSRLVLVAPRVPHVQSDPEAIAMASGADDVLASAHVVPTLGDALSGVHWSIAL
HHHHHCCCCEEEEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TARTREYGPPRLAPRAAATQACAQVGTGDIALVFGNERTGLANEHVEQCSALAHIPANPA
EEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
YSSLNLAQAVQVLAYELRVAFLEHASEPSAQPSGEAGTLAQSDEIERMYVHLENALIALD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEEE
FLDPRNPKKLMPRLRRLFARTGLEREEVNILRGVAKHILLKSGKPDGDA
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA