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The map label for this gene is tilS [H]

Identifier: 78066850

GI number: 78066850

Start: 2447161

End: 2448582

Strand: Reverse

Name: tilS [H]

Synonym: Bcep18194_A5381

Alternate gene names: 78066850

Gene position: 2448582-2447161 (Counterclockwise)

Preceding gene: 78066851

Following gene: 78066849

Centisome position: 66.28

GC content: 71.38

Gene sequence:

>1422_bases
GTGATTCCGCCAACCGAATTCTCCGCCGACCGCGTCGTCCTCGACGCGGTCGGCGTTGCGCTTTCCGGCATGGCGCCCGA
TGCACGCATCTCGATCGCGTACAGCGGCGGCCTCGACTCGACGGTGCTGCTCGACGCGGCCGTGCGCGTCGCGGGCGCGT
CGCGCTGCGTCGCGCTGCACGTGCATCACGGGCTGAGTGCGAATGCCGACGCGTGGGTTGCGCATGCCGAGGCGAGTGCG
GCCCGGCTCGGTGTCGCATTCGAGTCGATGCACGTCGACGTGCCGCGCGACAGCGGCCTCGGTGTCGAGGCGACGGCGCG
CGAGCGACGCTATGCGGCGCTCGACGAGATGTGCGAACGCCATGGCGTGGCCACGCTATGGCTCGCGCAGCATGCGGACG
ACCAGGCCGAGACCGTGTTGCTGCAGTTGTTGCGCGGCGCGGGCATCGCGGGGCTTGCCGCAATGGCGCCGCGTTACCGC
CCTGACGGCGTGAGCGTCGAGCGTGTGCGTCCGCTGCTGCGACTGTTGCGGGCGCAACTCGAGCGCTATGCGACGCAGCA
CGACCTGGCATGGATCGACGACGAATCGAACCATGACACGCGTTACGCGCGCAATGCGCTGCGCATCGACGTGCTGCCGG
CGCTGGCCGTGCATTTCCCGGGCTTTCGCGACGCGCTCGGCCGGGCGGCCCAGCACGCGGCGGCCGCACAGCGACTGCTC
GACGATCTGGCCGAACTCGACTTCGCCGCTGCCGCGCGCGATGACGGGCAGGCGCTGTCGCGCGACGCACTGGTCGCGTT
CGACGATACGCGCGGCGCGAACCTGCTGCGCTTCTGGATGCGCCGGCTCGGTCTGCCGGGTGCGTCGGCCGCCCGGCTCG
CCAACATGATGCGACAGCTACGTGCCACCAATGTCGCGCATGCGCTGCGCGTCGATCACGCGGGGCAATGCCTGCGCCTG
TATCGCGATGTCGTCTACTGGGAAGCGGGCGACAGCAGCGAACCGGCCGATGACGGCACTGGGCGCCCGCATCCGGAATC
GATGCTCGCGTGGGACGGGCAGGAGATCTGGCACCTGCCGGGTTGGCGCGGCACGTTCGTGTTCGCGCCGGCCGATGCGG
GAAGCGCCGACGCGGTGCCGGAGACGCTGCTGCGTGGCGCGCGGCTGGCGGCGCGCGCCCGCGCGGGCGGCGAGCGGATG
CGCACGTCGCCGGGCGGCCCGGGCCGTACGCTGAAGAACCTGTTCCAGGAGCGTGGCGTGCCGGCCTGGCAGCGCGACGT
GCCGCTGCTGTTCGCTGGTGACTCGCTGATCTACGTGCCGCGCCTTGGCGTGAATCGCGATGCCGGCTTGTCCGACGGCG
GGGCGTCCGGTGATGGCGCATGGCGCCGGATCGAGTGGCGTCCGGACCTGCTGATCGCATGA

Upstream 100 bases:

>100_bases
TTCGAGCGCCTGATGGCCTACGGCAAGTTCAAGGAAACCACGCCGGGCGCATGACCGGCTGCGTCCTCCATTTGCGCGCC
GCTGCGCGCGCGTTCCTCTC

Downstream 100 bases:

>100_bases
CGGCGGCAGGCTGACGGCGGCGCGATGGCGCGCGCATTTTCACCATTTGGTAAACAGCGCCGGAACGGCCCCGGACGCCG
CGAGCGGCTTGTCAAGCGGG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MIPPTEFSADRVVLDAVGVALSGMAPDARISIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEASA
ARLGVAFESMHVDVPRDSGLGVEATARERRYAALDEMCERHGVATLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSVERVRPLLRLLRAQLERYATQHDLAWIDDESNHDTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGQALSRDALVAFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRATNVAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGRPHPESMLAWDGQEIWHLPGWRGTFVFAPADAGSADAVPETLLRGARLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDSLIYVPRLGVNRDAGLSDGGASGDGAWRRIEWRPDLLIA

Sequences:

>Translated_473_residues
MIPPTEFSADRVVLDAVGVALSGMAPDARISIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEASA
ARLGVAFESMHVDVPRDSGLGVEATARERRYAALDEMCERHGVATLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSVERVRPLLRLLRAQLERYATQHDLAWIDDESNHDTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGQALSRDALVAFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRATNVAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGRPHPESMLAWDGQEIWHLPGWRGTFVFAPADAGSADAVPETLLRGARLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDSLIYVPRLGVNRDAGLSDGGASGDGAWRRIEWRPDLLIA
>Mature_473_residues
MIPPTEFSADRVVLDAVGVALSGMAPDARISIAYSGGLDSTVLLDAAVRVAGASRCVALHVHHGLSANADAWVAHAEASA
ARLGVAFESMHVDVPRDSGLGVEATARERRYAALDEMCERHGVATLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYR
PDGVSVERVRPLLRLLRAQLERYATQHDLAWIDDESNHDTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
DDLAELDFAAAARDDGQALSRDALVAFDDTRGANLLRFWMRRLGLPGASAARLANMMRQLRATNVAHALRVDHAGQCLRL
YRDVVYWEAGDSSEPADDGTGRPHPESMLAWDGQEIWHLPGWRGTFVFAPADAGSADAVPETLLRGARLAARARAGGERM
RTSPGGPGRTLKNLFQERGVPAWQRDVPLLFAGDSLIYVPRLGVNRDAGLSDGGASGDGAWRRIEWRPDLLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=450, Percent_Identity=36.2222222222222, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51088; Mature: 51088

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPPTEFSADRVVLDAVGVALSGMAPDARISIAYSGGLDSTVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWVAHAEASAARLGVAFESMHVDVPRDSGLGVEATARERRYAALDEMCER
EECCCCCCCCCEEEECCHHHHHHHEEEEHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
HGVATLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGVSVERVRPLLRLLRAQL
CCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYATQHDLAWIDDESNHDTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHCCCEEECCCCCCCHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAAAARDDGQALSRDALVAFDDTRGANLLRFWMRRLGLPGASAARLANMMRQL
HHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
RATNVAHALRVDHAGQCLRLYRDVVYWEAGDSSEPADDGTGRPHPESMLAWDGQEIWHLP
HHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHEECCCCEEECC
GWRGTFVFAPADAGSADAVPETLLRGARLAARARAGGERMRTSPGGPGRTLKNLFQERGV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCC
PAWQRDVPLLFAGDSLIYVPRLGVNRDAGLSDGGASGDGAWRRIEWRPDLLIA
CCHHCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MIPPTEFSADRVVLDAVGVALSGMAPDARISIAYSGGLDSTVLLDAAVRVAGASRCVALH
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWVAHAEASAARLGVAFESMHVDVPRDSGLGVEATARERRYAALDEMCER
EECCCCCCCCCEEEECCHHHHHHHEEEEHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
HGVATLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPRYRPDGVSVERVRPLLRLLRAQL
CCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
ERYATQHDLAWIDDESNHDTRYARNALRIDVLPALAVHFPGFRDALGRAAQHAAAAQRLL
HHHHHHCCCEEECCCCCCCHHHHHHHHEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DDLAELDFAAAARDDGQALSRDALVAFDDTRGANLLRFWMRRLGLPGASAARLANMMRQL
HHHHHHHHHHHCCCCCHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
RATNVAHALRVDHAGQCLRLYRDVVYWEAGDSSEPADDGTGRPHPESMLAWDGQEIWHLP
HHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHEECCCCEEECC
GWRGTFVFAPADAGSADAVPETLLRGARLAARARAGGERMRTSPGGPGRTLKNLFQERGV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCC
PAWQRDVPLLFAGDSLIYVPRLGVNRDAGLSDGGASGDGAWRRIEWRPDLLIA
CCHHCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA